Head-splitting is a prevalent physiological disorder in cabbage that causes substantial economic losses.However,the genetic factors and molecular mechanisms underlying head-splitting resistance remain largely unexplor...Head-splitting is a prevalent physiological disorder in cabbage that causes substantial economic losses.However,the genetic factors and molecular mechanisms underlying head-splitting resistance remain largely unexplored.This study identified a genomic region(q NLQ3.1) for head-splitting resistance on chromosome C03 through the combination of QTLseq and GPS analysis in an F_(2) population derived from hybridizing two cabbage inbred lines,‘Dazhengfu'(ZF,susceptible) and ‘103'(resistant).Traditional genetic linkage analysis narrowed q NLQ3.1 to a 74.6 kb region.Furthermore,comparative analysis of the two parental lines using transcriptomic and metabolic profiling demonstrated the crucial role of hormones in regulating head-splitting resistance.Bol028000,encoding a homologue of Arabidopsis Cytokinin Response Factor 3(CRF3),emerged as a promising candidate for head-splitting resistance and was subsequently validated through Sanger sequencing and quantitative RT-PCR(qRT-PCR).Subcellular localisation analysis revealed that Bol028000 was mainly expressed in the nucleus.Additionally,one kompetitive allele-specific PCR(KASP) marker from Bol028000 was developed and utilized to screen 42 inbred lines.These findings enhance the theoretical understanding of head-splitting resistance and provide valuable insights for the molecular breeding of head-splitting resistant cabbages.展开更多
Aquila Optimizer(AO)is a recently proposed population-based optimization technique inspired by Aquila’s behavior in catching prey.AO is applied in various applications and its numerous variants were proposed in the l...Aquila Optimizer(AO)is a recently proposed population-based optimization technique inspired by Aquila’s behavior in catching prey.AO is applied in various applications and its numerous variants were proposed in the literature.However,chaos theory has not been extensively investigated in AO.Moreover,it is still not applied in the parameter estimation of electro-hydraulic systems.In this work,ten well-defined chaotic maps were integrated into a narrowed exploitation of AO for the development of a robust chaotic optimization technique.An extensive investigation of twenty-three mathematical benchmarks and ten IEEE Congress on Evolutionary Computation(CEC)functions shows that chaotic Aquila optimization techniques perform better than the baseline technique.The investigation is further conducted on parameter estimation of an electro-hydraulic control system,which is performed on various noise levels and shows that the proposed chaotic AO with Piecewise map(CAO6)achieves the best fitness values of and at noise levels and respectively.Friedman test 2.873E-05,1.014E-04,8.728E-031.300E-03,1.300E-02,1.300E-01,for repeated measures,computational analysis,and Taguchi test reflect the superiority of CAO6 against the state of the arts,demonstrating its potential for addressing various engineering optimization problems.However,the sensitivity to parameter tuning may limit its direct application to complex optimization scenarios.展开更多
This research presents an advanced study on the modeling and stability analysis of electro-hydraulic control modules used in intelligent chassis systems.Firstly,a comprehensive nonlinear mathematical model of the elec...This research presents an advanced study on the modeling and stability analysis of electro-hydraulic control modules used in intelligent chassis systems.Firstly,a comprehensive nonlinear mathematical model of the electro-hydraulic power-shift system is developed,incorporating pipeline characteristics through impedance analysis and examining coupling effects between the pilot solenoid valve,main valve,and pipeline.Then,the model’s accuracy is validated through experimental testing,demonstrating high precision and minimal model errors.A comparative analysis between simulation data(both with and without pipeline characteristics)and experimental results reveals that the model considering pipeline parameters aligns more closely with experimental data,highlighting its superior accuracy.The research further explores the influence of key factors on system stability,including damping coefficient,feedback cavity orifice diameter,spring stiffness,pipeline length,and pipeline diameter.Significant findings include the critical impact of damping coefficient,orifice diameter,and pipeline length on stability,while spring stiffness has a minimal effect.These findings provide valuable insights for optimizing electro-hydraulic control modules in intelligent chassis systems,with practical implications for automotive and construction machinery applications.展开更多
Hydraulic-electric systems are widely utilized in various applications.However,over time,these systems may encounter random faults such as loose cables,ambient environmental noise,or sensor aging,leading to inaccurate...Hydraulic-electric systems are widely utilized in various applications.However,over time,these systems may encounter random faults such as loose cables,ambient environmental noise,or sensor aging,leading to inaccurate sensor readings.These faults may result in system instability or compromise safety.In this paper,we propose a fault compensation control system to mitigate the effects of sensor faults and ensure system safety.Specifically,we utilize the pressure sensor within the system to implement the control process and evaluate performance based on the piston position.First,we develop a mathematical model to identify optimal parameters for the fault estimation model based on the Lyapunov stability principle.Next,we design an unknown input observer that estimates the state vector and detects pressure sensor faults using a linear matrix inequality optimization algorithm.The estimated pressure faults are incorporated into the fault compensation control system to counteract their effects via a fault residual coefficient.The discrepancy between the feedback state and the estimated state determines this coefficient.We assess the piston position’s performance through pressure control to evaluate the proposed model’s effectiveness.Finally,the system simulation results are analyzed to validate the efficiency of the proposed model.When a pressure sensor fault occurs,the proposed approach effectively minimizes position control errors,enhancing overall system stability.When a pressure sensor fault occurs,the proposed model compensates for the fault to mitigate the impact of pressure problem,thereby enhancing the position control quality of the EHA system.The fault compensation method ensures over 90%system performance,with its effectiveness becoming more evident under pressure sensor faults.展开更多
Magnetization convenience is crucial consideration for design of valve magnetic actuators.The existing repulsive-magnetic-coupling of 2D maglev valve is not oriented to the integral-magnetization-processes,resulting i...Magnetization convenience is crucial consideration for design of valve magnetic actuators.The existing repulsive-magnetic-coupling of 2D maglev valve is not oriented to the integral-magnetization-processes,resulting in the high assembly cost.This paper presents a novel tractive-magnetic-coupling(TMC)and its application on a 2D electro-hydraulic proportional flow valve(2D-EHPFV),whose configuration not only fulfill the requirements of 2D valve,but also oriented to integral-magnetization-process.To investigate the output torque of TMC,a detailed analytical model considering leakage flux,edge effect and tooth magnetic saturation is formulated based on the equivalent magnetic circuit method.To facilitate the magnetic saturation calculation,a special magnetic permeability database is established for tooth region of TMC using Ansoft/Maxwell software.Prototype of TMC is machined and an exclusive experimental platform is built.Torque-displacement characteristics under different working airgap and tooth number are measured.The experimental results are in good agreement with the analytical results,which verifies the correctness of the analytical model.Then the TMC is integrated into the 2D-EHPFV by replacing the repulsive-magnetic-coupling.Prototype of 2D-EHPFV is designed and manufactured to test the no-load flow characteristics,load flow characteristics,leakage characteristics,frequency characteristics and step response.Under working pressure of 15 MPa,the maximum no-load flow rate is 82.2 L/min with the hysteresis of 2.6%,and the amplitude and phase frequency width is 21.6 Hz,and 28.9 Hz.The detailed experimental results show that TMC can be applied to 2D valves to form 2D-EHPFV,which can reduce hysteresis and cost,and improve response speed.展开更多
The aim of the study is to investigate the impact of the buffer groove structure on the pressure of continuous rotation electro-hydraulic servo motor.The mathematical model of the motor valve plate with triangular gro...The aim of the study is to investigate the impact of the buffer groove structure on the pressure of continuous rotation electro-hydraulic servo motor.The mathematical model of the motor valve plate with triangular groove and U-groove structure is established firstly,and the structure size of the two buffer grooves with better pressure drop effect is obtained by Matlab.Secondly,an established pressure gradient model is developed for the sealed canisters for electric motors using a combined groove structure.The bird swarm optimization algorithm is used to obtain the optimal dimensions for the combined depth and angle of the pressure groove.The flow field in the motor seal chamber is simulated and calculated by Fluent.This study compared the pressure field distributions in the motors sealing chamber using triangular and combined groove structures.It investigated the combined grooves effect on the pressure impact during the commutation of a continuously rotating electro-hydraulic servo motor.It is found that the combined groove structure has a positive impact on reducing the pressure impact.The results indicate that the combined groove structure significantly enhances the efficiency of mitigating pressure shocks when the motor switches between high-and low-pressure chambers.展开更多
The current study conducted the psychological capital profiles and the relation between profile memberships and learning burnout among undergraduates.Participants were 541 Chinese undergraduates ranging from 18 to 21 ...The current study conducted the psychological capital profiles and the relation between profile memberships and learning burnout among undergraduates.Participants were 541 Chinese undergraduates ranging from 18 to 21 years old(48.2%male;Mean years=19.54,SD=1.09 years).Latent profile analysis revealed three categories of psychological capital profile consistent high psychological capital profile(50.5%),consistent low psychological capital profile(38.1%),and dominate loss-orientated psychological capital profile(11.4%).The undergraduates in consistent high profile reported high self-efficacy,resilience,hope,and optimism.Consistent low profile characterized by a little low(~0.50 SD below the M)self-efficacy,resilience,hope,and optimism.Dominant loss-orientated profile displayed low self-efficacy,hope,optimism,but a little relatively high resilience.Specifically,undergraduates in consistent high profile exhibited low level of learning burnout;while undergraduates with low level of psychological capital(i.e.,consistent low profile and dominate loss-orientated profile)reported high level of learning burnout.The findings have theoretical significance which provides important knowledge about psychological capital profile among Chinese undergraduates.They have practical implications for student counselling and development providing person-centric support to students based on their individual psychological profiles.展开更多
In-depth study of the components of polymyxins is the key to controlling the quality of this class of antibiotics.Similarities and variations of components present significant analytical challenges.A two-dimensional(2...In-depth study of the components of polymyxins is the key to controlling the quality of this class of antibiotics.Similarities and variations of components present significant analytical challenges.A two-dimensional(2D)liquid chromatography-mass spectrometry(LC-MS)method was established for screening and comprehensive profiling of compositions of the antibiotic colistimethate sodium(CMS).A high concentration of phosphate buffer mobile phase was used in the first-dimensional LC system to get the components well separated.For efficient and high-accuracy screening of CMS,a targeted method based on a self-constructed high resolution(HR)mass spectrum database of CMS components was established.The database was built based on the commercial MassHunter Personal Compound Database and Library(PCDL)software and its accuracy of the compound matching result was verified with six known components before being applied to genuine sample screening.On this basis,the unknown peaks in the CMS chromatograms were deduced and assigned.The molecular formula,group composition,and origins of a total of 99 compounds,of which the combined area percentage accounted for more than 95%of CMS components,were deduced by this 2D-LC-MS method combined with the MassHunter PCDL.This profiling method was highly efficient and could distinguish hundreds of components within 3 h,providing reliable results for quality control of this kind of complex drugs.展开更多
Objective:While immunotherapy holds great potential for triple-negative breast cancer(TNBC),the lack of non-invasive biomarkers to identify beneficiaries limits the application.Methods:Paired baseline,on-treatment,and...Objective:While immunotherapy holds great potential for triple-negative breast cancer(TNBC),the lack of non-invasive biomarkers to identify beneficiaries limits the application.Methods:Paired baseline,on-treatment,and post-treatment plasma samples were collected from 195 TNBC patients receiving anti-PD-1 immunotherapy in this retrospective study conducted at the Fudan University Shanghai Cancer Center(FUSCC)for sequential high-precision proteomic profiling.Results:ARG1,NOS3,and CD28 were identified as plasma proteins significantly associated with the response to immunotherapy in neoadjuvant settings or in advanced stages of TNBC.Matched single-cell RNA sequencing data were incorporated to correlate peripheral plasma with the tumor microenvironment.Furthermore,the Plasma Immuno Prediction Score was developed to demonstrate significant predictive power for evaluating the efficacy and prognosis of patients undergoing neoadjuvant immunotherapy.Conclusions:The results underscore the importance of systemic immunity in the immunotherapy response and support the use of plasma protein profiles as a feasible tool for enhancing personalized management of immunotherapy in breast cancer.展开更多
Bigeye tuna is a protein-rich fish that is susceptible to spoilage during cold storage,however,there is limited information on untargeted metabolomic profiling of bigeye tuna concerning spoilage-associated enzymes and...Bigeye tuna is a protein-rich fish that is susceptible to spoilage during cold storage,however,there is limited information on untargeted metabolomic profiling of bigeye tuna concerning spoilage-associated enzymes and metabolites.This study aimed to investigate how cold storage affects enzyme activities,nutrient composition,tissue microstructures and spoilage metabolites of bigeye tuna.The activities of cathepsins B,H,L increased,while Na^(+)/K^(+)-ATPase and Mg^(2+)-ATPase decreased,α-glucosidase,lipase and lipoxygenase first increased and then decreased during cold storage,suggesting that proteins undergo degradation and ATP metabolism occurs at a faster rate during cold storage.Nutrient composition(moisture and lipid content),total amino acids decreased,suggesting that the nutritional value of bigeye tuna was reduced.Besides,a logistic regression equation has been established as a food analysis tool and assesses the dynamics and correlation of the enzyme of bigeye tuna during cold storage.Based on untargeted metabolomic profiling analysis,a total of 524 metabolites were identified in the bigeye tuna contained several spoilage metabolites involved in lipid metabolism(glycerophosphocholine and choline phosphate),amino acid metabolism(L-histidine,5-deoxy-5′-(methylthio)adenosine,5-methylthioadenosine),carbohydrate metabolism(D-gluconic acid,α-D-fructose 1,6-bisphosphate,D-glyceraldehyde 3-phosphate).The results of tissue microstructures of tuna showed a looser network and visible deterioration of tissue fiber during cold storage.Therefore,metabolomic analysis and tissue microstructures provide insight into the spoilage mechanism investigations on bigeye tuna during cold storage.展开更多
Objectives:Despite the considerable regenerative capacity exhibited by adipose-derived mesenchymal stem cells(ASCs),their genetic and molecular mechanisms remain incompletely understood.Methods:In this study,we analyz...Objectives:Despite the considerable regenerative capacity exhibited by adipose-derived mesenchymal stem cells(ASCs),their genetic and molecular mechanisms remain incompletely understood.Methods:In this study,we analyzed the global gene expression profile of adipose-derived mesenchymal stem cells(ASCs)using microarray analysis and compared it with stromal vascular fraction(SVF)cells.Results:Microarray analysis revealed that ASCs express elevated levels of genes related to the extracellular matrix(ECM;extracellular matrix)and collagen,which are critical components of tissue remodeling and wound healing.Additionally,genes associated with cell growth,differentiation,motility,and plasticity were highly expressed.When compared to stromal vascular fraction(SVF)cells,ASCs demonstrated enrichment of genes involved in anti-inflammatory responses,immune modulation,tissue repair,cell adhesion,and migration processes.Gene Set Enrichment Analysis(GSEA;Gene Set Enrichment Analysis)showed activation of pathways related to angiogenesis,such as vascular endothelial growth factor(VEGF),Integrin,Wnt signaling pathways,transforming growth factor-beta(TGF-β),extracellular matrix(ECM),and matrix metalloproteinase(MMP),highlighting the significant angiogenic potential of ASCs.Gene Ontology(GO;Gene Ontology)analysis further linked ASCs to biological processes associated with the regulation of cell proliferation and muscle cell differentiation.Conclusion:These findings collectively underscore the suitability of adipose-derived mesenchymal stem cells(ASCs)as a promising candidate for regenerative medicine,particularly in applications involving tissue repair,immune modulation,and promotion of angiogenesis.展开更多
Pigeons and certain other avian species produce a milklike secretion in their crop sacs to nourish offspring,yet the detailed processes involved are not fully elucidated.This study investigated the crop sacs of 225-da...Pigeons and certain other avian species produce a milklike secretion in their crop sacs to nourish offspring,yet the detailed processes involved are not fully elucidated.This study investigated the crop sacs of 225-day-old unpaired non-lactating male pigeons(MN)and males initiating lactation on the first day after incubation(ML).Using RNA sequencing,ribosomeprofiling,andsingle-cell transcriptome sequencing(scRNA-seq),we identified a significant up-regulation of genes associated with ribosome assembly and protein synthesis in ML compared to MN.Results from scRNA-seq analysis identified 12distinct cell types and 22 clusters,with secretory epithelial cells(SECs)exhibiting marked expression of plasma cell markers,including IGLL1 and MZB1.RNA fluorescence in situ hybridization(RNA FISH)and IgY quantification confirmed the critical role of SECs in producing endogenous IgY during lactation.We propose that fibroblast-derived BAFF signals activate SECs,mimicking B cell transformation and enhancing protein production through the unfolded protein response(UPR).These findings shed light on the cellular dynamics of pigeon milk production and contribute to a broader understanding of avian biology.展开更多
Global warming underscores the urgent need to enhance carbon sinks to mitigate climate change,yet the role of coastal shellfish aquaculture area as either carbon sinks or sources remains unclear.In this study,we condu...Global warming underscores the urgent need to enhance carbon sinks to mitigate climate change,yet the role of coastal shellfish aquaculture area as either carbon sinks or sources remains unclear.In this study,we conducted high-resolution profiling observations of CO_(2)dynamics and net community production(NCP)in a mussel farm located in the Changjiang(Yangtze)River estuary(CRE)during early autumn.Results indicate that the partial pressure of CO_(2)(pCO_(2))in the water column was consistently higher than the atmospheric level,averaging 74.75±2.44 Pa,signaling that the mussel farm acted as a CO_(2)source.The average air-water CO_(2)exchange flux(FCO_(2))averaged-1.91±1.16 mmol/(m^(2)·h),and NCP in the water column(NCPCOL)averaged-6.66±12.81 mmol/(m^(2)·h),revealing a heterotrophic condition.A significant inverse correlation was found between NCPCOLand sea surface pCO_(2)with a lag time of approximately 3.31 h,highlighting the influence of biological activity in modulating CO_(2)dynamics.Physical processes such as freshwater inputs,vertical mixing,and wind patterns,also played a crucial role in shaping pCO_(2)variations.The study emphasizes the complexity of CO_(2)dynamics in costal aquaculture areas and the need for long-term,high-resolution monitoring to better understand their contribution to climate change mitigation.The high-resolution profiling system provided novel insights into the dynamics of NCP and CO_(2)fluxes,showing its potential as an advanced tool for studying aquaculture systems.Future research should focus on expanding the geographic and temporal scope of studies,combining observational and modeling approaches to better predict the responses of aquaculture systems to environmental changes.展开更多
An enhanced least mean square(LMS)error identification algorithm integrated with Kalman filtering is proposed to resolve accuracy degradation induced by nonlinear dynamics and parameter uncertainties in continuous rot...An enhanced least mean square(LMS)error identification algorithm integrated with Kalman filtering is proposed to resolve accuracy degradation induced by nonlinear dynamics and parameter uncertainties in continuous rotary electro-hydraulic servo systems.This enhancement accelerates convergence and improves accuracy compared with traditional LMS.A fifth-order identification mod-el is developed based on valve-controlled hydraulic motors,with parameters identified using Kalman filter state estimation and gradient smoothing.The results indicate that the improved LMS effectively enhances parameter identification.An advanced disturbance rejection controller(ADRC)is de-signed,and its performance is compared with an optimal proportional integral derivative(PID)con-troller through Simulink simulations.The results show that the ADRC fulfills the control specifications and expands the system’s operational bandwidth.展开更多
The current research of master cylinder pressure estimation mainly relies on hydraulic characteristic or vehicle dynamics.But they are not independently applicable to any environment and have their own scope of applic...The current research of master cylinder pressure estimation mainly relies on hydraulic characteristic or vehicle dynamics.But they are not independently applicable to any environment and have their own scope of application.In addition,about the master cylinder pressure control,there are few studies that can simultaneously balance pressure building accuracy,speed,and prevent pressure overshoot and jitter.In this paper,an adaptative fusion method based on electro-hydraulic characteristic and vehicle mode is proposed to estimate the master cylinder pressure.The fusion strategy is mainly based on the prediction performance of two algorithms under different vehicle speeds,pressures,and ABS states.Apart from this,this article also includes real-time prediction of the friction model based on RLS to improve the accuracy of the electro-hydraulic mode.In order to simultaneously balance pressure control accuracy,response speed,and prevent overshoot and jitter,this article proposes an adaptative LQR controller for MC pressure control which uses fuzzy-logic controller to adjust the weights of LQR controller based on target pressure and difference compared with actual pressure.Through mode-in-loop and hardware-in-loop tests in ramp,step and sinusoidal response,the whole estimation and control system is verified based on real hydraulic system and the performance is satisfactory under these scenes.This research proposes an adaptative pressure estimation and control architecture for integrated electro-hydraulic brake system which could eliminate pressure sensors in typical scenarios and ensure the comprehensive performance of pressure control.展开更多
Chronic diarrhea remains a frequent and debilitating complication among individuals living with HIV,particularly in the context of advanced immunosuppression.Its multifactorial nature often complicates both diagnosis ...Chronic diarrhea remains a frequent and debilitating complication among individuals living with HIV,particularly in the context of advanced immunosuppression.Its multifactorial nature often complicates both diagnosis and treatment.We present insights from our recent study investigating gut microbial alterations using 16S rRNA sequencing in HIV-positive patients with diarrheal syndrome of unclear etiology.展开更多
Objective:To evaluate the quality of Lygodium japonicum(Thunb.)Sw.(L.japonicum,Hai Jin Sha)by comparing its components without stewed(W)and stewed(S)using ultra-high-performance liquid chromatography(UHPLC)and chemome...Objective:To evaluate the quality of Lygodium japonicum(Thunb.)Sw.(L.japonicum,Hai Jin Sha)by comparing its components without stewed(W)and stewed(S)using ultra-high-performance liquid chromatography(UHPLC)and chemometric analysis.Additionally,network pharmacology was employed to investigate the possible mechanisms of action of L.japonicum in the urinary calculi(UC)treatment.Methods:A fingerprinting method was established to identify components through UHPLC-tandem mass spectrometry.Chemometric techniques were used to compare the L.japonicum extraction methods.Furthermore,various network pharmacological approaches were used to identify and analyze the potential targets of the identified components in relation to UC.Results:The W and S extracts were distributed into two distinct clusters.Significant differences in the levels of protocatechuic aldehyde,caffeic acid,and p-coumaric acid were observed between S and W.Network pharmacology analysis revealed that the primary targets of L.japonicum in the UC treatment were serum albumin and epidermal growth factor receptors,with potential active components including protocatechuic acid and caffeic acid.Conclusion:This study comprehensively examined the therapeutic components of L.japonicum before and after boiling,shedding light on its potential mechanisms of action in UC treatment.These findings offer valuable insights into the development and utilization of L.japonicum resources.展开更多
Objectives:This study aimed to explore the effectiveness and advantages of an“Internet+”nursing model based on user profilingin the rehabilitation of postoperative breast cancer patients.Methods:Breast cancer patien...Objectives:This study aimed to explore the effectiveness and advantages of an“Internet+”nursing model based on user profilingin the rehabilitation of postoperative breast cancer patients.Methods:Breast cancer patients admitted to the hospital from July 2023 to September 2024 were enrolled.These patients were randomly assigned to a control group and an intervention group,with 52 patients in each group.The control group received routine nursing care,while the intervention group received an“Internet+”nursing intervention based on user profilingin addition to routine care.The intervention period lasted for one month following discharge.Before and one month after the intervention,the Fear of Progression Questionnaire-Short Form(FOP-Q-SF),the Fear of Cancer Recurrence Inventory-Short Form(FCRI-SF),Chinese Posttraumatic Growth Inventory(C-PTGI),and the Functional Assessment of Cancer Therapy-Breast(FACT-B)were applied to assess the effects of interventions.Results:A total of 104 patients were analyzed.After the intervention,FOP-Q-SF and FCRI-SF scores were significantlylower in the intervention group compared to the control group,with statistical significance(t=3.98,P<0.001;t=-7.59,P<0.001),and Cohen’s d of 0.781 and 1.49,respectively.Additionally,CPTGI and FACT-B scores in the intervention group were significantly higher than those in the control group(t=-6.534,P<0.001;t=-4.579,P<0.001),with Cohen’s d of 0.585 and 0.656.Conclusions:An“Internet+”nursing model based on user profilingcould reduce postoperative breast cancer patients fear of disease progression and cancer recurrence,also enhancing posttraumatic growth and overall quality of life.展开更多
基金funded by the Agricultural Development Program through Science and Technology of Shanghai,China (2022-02-08-00-12-F01099)the National Key R&D Program of China (2023YFD1201501)+1 种基金the Science and Technology Commission of Shanghai Municipality,China (22DX1900100)the Excellent Team Project of Shanghai Academy of Agricultural Sciences,China (2022(007))。
文摘Head-splitting is a prevalent physiological disorder in cabbage that causes substantial economic losses.However,the genetic factors and molecular mechanisms underlying head-splitting resistance remain largely unexplored.This study identified a genomic region(q NLQ3.1) for head-splitting resistance on chromosome C03 through the combination of QTLseq and GPS analysis in an F_(2) population derived from hybridizing two cabbage inbred lines,‘Dazhengfu'(ZF,susceptible) and ‘103'(resistant).Traditional genetic linkage analysis narrowed q NLQ3.1 to a 74.6 kb region.Furthermore,comparative analysis of the two parental lines using transcriptomic and metabolic profiling demonstrated the crucial role of hormones in regulating head-splitting resistance.Bol028000,encoding a homologue of Arabidopsis Cytokinin Response Factor 3(CRF3),emerged as a promising candidate for head-splitting resistance and was subsequently validated through Sanger sequencing and quantitative RT-PCR(qRT-PCR).Subcellular localisation analysis revealed that Bol028000 was mainly expressed in the nucleus.Additionally,one kompetitive allele-specific PCR(KASP) marker from Bol028000 was developed and utilized to screen 42 inbred lines.These findings enhance the theoretical understanding of head-splitting resistance and provide valuable insights for the molecular breeding of head-splitting resistant cabbages.
基金funded by Taif University,Saudi Arabia,Project No.(TU-DSPP-2024-52).
文摘Aquila Optimizer(AO)is a recently proposed population-based optimization technique inspired by Aquila’s behavior in catching prey.AO is applied in various applications and its numerous variants were proposed in the literature.However,chaos theory has not been extensively investigated in AO.Moreover,it is still not applied in the parameter estimation of electro-hydraulic systems.In this work,ten well-defined chaotic maps were integrated into a narrowed exploitation of AO for the development of a robust chaotic optimization technique.An extensive investigation of twenty-three mathematical benchmarks and ten IEEE Congress on Evolutionary Computation(CEC)functions shows that chaotic Aquila optimization techniques perform better than the baseline technique.The investigation is further conducted on parameter estimation of an electro-hydraulic control system,which is performed on various noise levels and shows that the proposed chaotic AO with Piecewise map(CAO6)achieves the best fitness values of and at noise levels and respectively.Friedman test 2.873E-05,1.014E-04,8.728E-031.300E-03,1.300E-02,1.300E-01,for repeated measures,computational analysis,and Taguchi test reflect the superiority of CAO6 against the state of the arts,demonstrating its potential for addressing various engineering optimization problems.However,the sensitivity to parameter tuning may limit its direct application to complex optimization scenarios.
基金Supported by the Basic Product Innovation Plan for Vehicle Power Scientific Research Project(Grant No.JCCPCX201704).
文摘This research presents an advanced study on the modeling and stability analysis of electro-hydraulic control modules used in intelligent chassis systems.Firstly,a comprehensive nonlinear mathematical model of the electro-hydraulic power-shift system is developed,incorporating pipeline characteristics through impedance analysis and examining coupling effects between the pilot solenoid valve,main valve,and pipeline.Then,the model’s accuracy is validated through experimental testing,demonstrating high precision and minimal model errors.A comparative analysis between simulation data(both with and without pipeline characteristics)and experimental results reveals that the model considering pipeline parameters aligns more closely with experimental data,highlighting its superior accuracy.The research further explores the influence of key factors on system stability,including damping coefficient,feedback cavity orifice diameter,spring stiffness,pipeline length,and pipeline diameter.Significant findings include the critical impact of damping coefficient,orifice diameter,and pipeline length on stability,while spring stiffness has a minimal effect.These findings provide valuable insights for optimizing electro-hydraulic control modules in intelligent chassis systems,with practical implications for automotive and construction machinery applications.
基金supported by Nguyen Tat Thanh University,Ho Chi Minh City,Vietnam,provided with the facilities required to carry out this work.
文摘Hydraulic-electric systems are widely utilized in various applications.However,over time,these systems may encounter random faults such as loose cables,ambient environmental noise,or sensor aging,leading to inaccurate sensor readings.These faults may result in system instability or compromise safety.In this paper,we propose a fault compensation control system to mitigate the effects of sensor faults and ensure system safety.Specifically,we utilize the pressure sensor within the system to implement the control process and evaluate performance based on the piston position.First,we develop a mathematical model to identify optimal parameters for the fault estimation model based on the Lyapunov stability principle.Next,we design an unknown input observer that estimates the state vector and detects pressure sensor faults using a linear matrix inequality optimization algorithm.The estimated pressure faults are incorporated into the fault compensation control system to counteract their effects via a fault residual coefficient.The discrepancy between the feedback state and the estimated state determines this coefficient.We assess the piston position’s performance through pressure control to evaluate the proposed model’s effectiveness.Finally,the system simulation results are analyzed to validate the efficiency of the proposed model.When a pressure sensor fault occurs,the proposed approach effectively minimizes position control errors,enhancing overall system stability.When a pressure sensor fault occurs,the proposed model compensates for the fault to mitigate the impact of pressure problem,thereby enhancing the position control quality of the EHA system.The fault compensation method ensures over 90%system performance,with its effectiveness becoming more evident under pressure sensor faults.
基金Supported by National Natural Science Foundation of China(Grant Nos.51975524,52375067)Zhejiang Provincial Natural Science Foundation of China(Grant No.Y23E050014).
文摘Magnetization convenience is crucial consideration for design of valve magnetic actuators.The existing repulsive-magnetic-coupling of 2D maglev valve is not oriented to the integral-magnetization-processes,resulting in the high assembly cost.This paper presents a novel tractive-magnetic-coupling(TMC)and its application on a 2D electro-hydraulic proportional flow valve(2D-EHPFV),whose configuration not only fulfill the requirements of 2D valve,but also oriented to integral-magnetization-process.To investigate the output torque of TMC,a detailed analytical model considering leakage flux,edge effect and tooth magnetic saturation is formulated based on the equivalent magnetic circuit method.To facilitate the magnetic saturation calculation,a special magnetic permeability database is established for tooth region of TMC using Ansoft/Maxwell software.Prototype of TMC is machined and an exclusive experimental platform is built.Torque-displacement characteristics under different working airgap and tooth number are measured.The experimental results are in good agreement with the analytical results,which verifies the correctness of the analytical model.Then the TMC is integrated into the 2D-EHPFV by replacing the repulsive-magnetic-coupling.Prototype of 2D-EHPFV is designed and manufactured to test the no-load flow characteristics,load flow characteristics,leakage characteristics,frequency characteristics and step response.Under working pressure of 15 MPa,the maximum no-load flow rate is 82.2 L/min with the hysteresis of 2.6%,and the amplitude and phase frequency width is 21.6 Hz,and 28.9 Hz.The detailed experimental results show that TMC can be applied to 2D valves to form 2D-EHPFV,which can reduce hysteresis and cost,and improve response speed.
基金Supported by the National Natural Science Foundation of China(No.52375037)the Masters Innovation Project of Beijing University of Civil Engineering and Architecture(No.PG2024142)+1 种基金the Outstanding Youth of Pyramid Talent Training Project of Beijing University of Civil Engineering and Architecture(No.GDRC20220801)the Breeding Program Funding of Beijing University of Civil Engineering and Architecture(No.X24026).
文摘The aim of the study is to investigate the impact of the buffer groove structure on the pressure of continuous rotation electro-hydraulic servo motor.The mathematical model of the motor valve plate with triangular groove and U-groove structure is established firstly,and the structure size of the two buffer grooves with better pressure drop effect is obtained by Matlab.Secondly,an established pressure gradient model is developed for the sealed canisters for electric motors using a combined groove structure.The bird swarm optimization algorithm is used to obtain the optimal dimensions for the combined depth and angle of the pressure groove.The flow field in the motor seal chamber is simulated and calculated by Fluent.This study compared the pressure field distributions in the motors sealing chamber using triangular and combined groove structures.It investigated the combined grooves effect on the pressure impact during the commutation of a continuously rotating electro-hydraulic servo motor.It is found that the combined groove structure has a positive impact on reducing the pressure impact.The results indicate that the combined groove structure significantly enhances the efficiency of mitigating pressure shocks when the motor switches between high-and low-pressure chambers.
基金supported by Hunan Province Education Department Outstanding Youth Project(23B1131)Hunan Province Education Science Fourteenth Five-Year Plan Project(XJK24BXL003).
文摘The current study conducted the psychological capital profiles and the relation between profile memberships and learning burnout among undergraduates.Participants were 541 Chinese undergraduates ranging from 18 to 21 years old(48.2%male;Mean years=19.54,SD=1.09 years).Latent profile analysis revealed three categories of psychological capital profile consistent high psychological capital profile(50.5%),consistent low psychological capital profile(38.1%),and dominate loss-orientated psychological capital profile(11.4%).The undergraduates in consistent high profile reported high self-efficacy,resilience,hope,and optimism.Consistent low profile characterized by a little low(~0.50 SD below the M)self-efficacy,resilience,hope,and optimism.Dominant loss-orientated profile displayed low self-efficacy,hope,optimism,but a little relatively high resilience.Specifically,undergraduates in consistent high profile exhibited low level of learning burnout;while undergraduates with low level of psychological capital(i.e.,consistent low profile and dominate loss-orientated profile)reported high level of learning burnout.The findings have theoretical significance which provides important knowledge about psychological capital profile among Chinese undergraduates.They have practical implications for student counselling and development providing person-centric support to students based on their individual psychological profiles.
基金support from the Science Research Program Project for Drug Regulation,Jiangsu Medical Products Administration,China(Grant No.:202207)the National Drug Standards Revision Project,China(Grant No.:2023Y41)+1 种基金the National Natural Science Foundation of China(Grant No.:22276080)the Foreign Expert Project,China(Grant No.:G2022014096L).
文摘In-depth study of the components of polymyxins is the key to controlling the quality of this class of antibiotics.Similarities and variations of components present significant analytical challenges.A two-dimensional(2D)liquid chromatography-mass spectrometry(LC-MS)method was established for screening and comprehensive profiling of compositions of the antibiotic colistimethate sodium(CMS).A high concentration of phosphate buffer mobile phase was used in the first-dimensional LC system to get the components well separated.For efficient and high-accuracy screening of CMS,a targeted method based on a self-constructed high resolution(HR)mass spectrum database of CMS components was established.The database was built based on the commercial MassHunter Personal Compound Database and Library(PCDL)software and its accuracy of the compound matching result was verified with six known components before being applied to genuine sample screening.On this basis,the unknown peaks in the CMS chromatograms were deduced and assigned.The molecular formula,group composition,and origins of a total of 99 compounds,of which the combined area percentage accounted for more than 95%of CMS components,were deduced by this 2D-LC-MS method combined with the MassHunter PCDL.This profiling method was highly efficient and could distinguish hundreds of components within 3 h,providing reliable results for quality control of this kind of complex drugs.
基金supported by the National Key Research and Development Project of China(Grant No.2021YFF1201300 and 2021YFF1201302)the Shanghai Committee of Science and Technology(Grant No.24DX2800100)the Institutional Projects of SIBPT(Grant No.YZ2024-07)。
文摘Objective:While immunotherapy holds great potential for triple-negative breast cancer(TNBC),the lack of non-invasive biomarkers to identify beneficiaries limits the application.Methods:Paired baseline,on-treatment,and post-treatment plasma samples were collected from 195 TNBC patients receiving anti-PD-1 immunotherapy in this retrospective study conducted at the Fudan University Shanghai Cancer Center(FUSCC)for sequential high-precision proteomic profiling.Results:ARG1,NOS3,and CD28 were identified as plasma proteins significantly associated with the response to immunotherapy in neoadjuvant settings or in advanced stages of TNBC.Matched single-cell RNA sequencing data were incorporated to correlate peripheral plasma with the tumor microenvironment.Furthermore,the Plasma Immuno Prediction Score was developed to demonstrate significant predictive power for evaluating the efficacy and prognosis of patients undergoing neoadjuvant immunotherapy.Conclusions:The results underscore the importance of systemic immunity in the immunotherapy response and support the use of plasma protein profiles as a feasible tool for enhancing personalized management of immunotherapy in breast cancer.
基金supported by the Shanghai Sailing Program(22YF1416300)Youth Fund Project of National Natural Science Foundation of China(32202117)+1 种基金National Key Research and Development Program of China(2022YFD2100104)the China Agriculture Research System(CARS-47).
文摘Bigeye tuna is a protein-rich fish that is susceptible to spoilage during cold storage,however,there is limited information on untargeted metabolomic profiling of bigeye tuna concerning spoilage-associated enzymes and metabolites.This study aimed to investigate how cold storage affects enzyme activities,nutrient composition,tissue microstructures and spoilage metabolites of bigeye tuna.The activities of cathepsins B,H,L increased,while Na^(+)/K^(+)-ATPase and Mg^(2+)-ATPase decreased,α-glucosidase,lipase and lipoxygenase first increased and then decreased during cold storage,suggesting that proteins undergo degradation and ATP metabolism occurs at a faster rate during cold storage.Nutrient composition(moisture and lipid content),total amino acids decreased,suggesting that the nutritional value of bigeye tuna was reduced.Besides,a logistic regression equation has been established as a food analysis tool and assesses the dynamics and correlation of the enzyme of bigeye tuna during cold storage.Based on untargeted metabolomic profiling analysis,a total of 524 metabolites were identified in the bigeye tuna contained several spoilage metabolites involved in lipid metabolism(glycerophosphocholine and choline phosphate),amino acid metabolism(L-histidine,5-deoxy-5′-(methylthio)adenosine,5-methylthioadenosine),carbohydrate metabolism(D-gluconic acid,α-D-fructose 1,6-bisphosphate,D-glyceraldehyde 3-phosphate).The results of tissue microstructures of tuna showed a looser network and visible deterioration of tissue fiber during cold storage.Therefore,metabolomic analysis and tissue microstructures provide insight into the spoilage mechanism investigations on bigeye tuna during cold storage.
基金supported through National Research Foundation(NRF)of Korea grants funded by the Korean Government(No.NRF-2022R1F1A1064405)the research fund of Catholic Kwandong University and Catholic Kwandong University International St.Mary’s Hospital for S.-W Kim.
文摘Objectives:Despite the considerable regenerative capacity exhibited by adipose-derived mesenchymal stem cells(ASCs),their genetic and molecular mechanisms remain incompletely understood.Methods:In this study,we analyzed the global gene expression profile of adipose-derived mesenchymal stem cells(ASCs)using microarray analysis and compared it with stromal vascular fraction(SVF)cells.Results:Microarray analysis revealed that ASCs express elevated levels of genes related to the extracellular matrix(ECM;extracellular matrix)and collagen,which are critical components of tissue remodeling and wound healing.Additionally,genes associated with cell growth,differentiation,motility,and plasticity were highly expressed.When compared to stromal vascular fraction(SVF)cells,ASCs demonstrated enrichment of genes involved in anti-inflammatory responses,immune modulation,tissue repair,cell adhesion,and migration processes.Gene Set Enrichment Analysis(GSEA;Gene Set Enrichment Analysis)showed activation of pathways related to angiogenesis,such as vascular endothelial growth factor(VEGF),Integrin,Wnt signaling pathways,transforming growth factor-beta(TGF-β),extracellular matrix(ECM),and matrix metalloproteinase(MMP),highlighting the significant angiogenic potential of ASCs.Gene Ontology(GO;Gene Ontology)analysis further linked ASCs to biological processes associated with the regulation of cell proliferation and muscle cell differentiation.Conclusion:These findings collectively underscore the suitability of adipose-derived mesenchymal stem cells(ASCs)as a promising candidate for regenerative medicine,particularly in applications involving tissue repair,immune modulation,and promotion of angiogenesis.
基金supported by the Department of Agriculture and Rural Affairs of Jiangxi Province,China (JXARS-09)Science and Technology Program of Guangdong Province,China (2020B1212060060)。
文摘Pigeons and certain other avian species produce a milklike secretion in their crop sacs to nourish offspring,yet the detailed processes involved are not fully elucidated.This study investigated the crop sacs of 225-day-old unpaired non-lactating male pigeons(MN)and males initiating lactation on the first day after incubation(ML).Using RNA sequencing,ribosomeprofiling,andsingle-cell transcriptome sequencing(scRNA-seq),we identified a significant up-regulation of genes associated with ribosome assembly and protein synthesis in ML compared to MN.Results from scRNA-seq analysis identified 12distinct cell types and 22 clusters,with secretory epithelial cells(SECs)exhibiting marked expression of plasma cell markers,including IGLL1 and MZB1.RNA fluorescence in situ hybridization(RNA FISH)and IgY quantification confirmed the critical role of SECs in producing endogenous IgY during lactation.We propose that fibroblast-derived BAFF signals activate SECs,mimicking B cell transformation and enhancing protein production through the unfolded protein response(UPR).These findings shed light on the cellular dynamics of pigeon milk production and contribute to a broader understanding of avian biology.
基金Supported by the National Natural Science Foundation of China(Nos.U23A2033,41976154)the Key R&D Program of Zhejiang Province,China(No.2023C03011)+2 种基金the Key R&D Program of Guangxi,China(No.Guike AB22080099)the Science Foundation of Donghai Laboratory(No.DH-2022KF0205)the Bureau of Science and Technology of Zhoushan(No.2022C81006)。
文摘Global warming underscores the urgent need to enhance carbon sinks to mitigate climate change,yet the role of coastal shellfish aquaculture area as either carbon sinks or sources remains unclear.In this study,we conducted high-resolution profiling observations of CO_(2)dynamics and net community production(NCP)in a mussel farm located in the Changjiang(Yangtze)River estuary(CRE)during early autumn.Results indicate that the partial pressure of CO_(2)(pCO_(2))in the water column was consistently higher than the atmospheric level,averaging 74.75±2.44 Pa,signaling that the mussel farm acted as a CO_(2)source.The average air-water CO_(2)exchange flux(FCO_(2))averaged-1.91±1.16 mmol/(m^(2)·h),and NCP in the water column(NCPCOL)averaged-6.66±12.81 mmol/(m^(2)·h),revealing a heterotrophic condition.A significant inverse correlation was found between NCPCOLand sea surface pCO_(2)with a lag time of approximately 3.31 h,highlighting the influence of biological activity in modulating CO_(2)dynamics.Physical processes such as freshwater inputs,vertical mixing,and wind patterns,also played a crucial role in shaping pCO_(2)variations.The study emphasizes the complexity of CO_(2)dynamics in costal aquaculture areas and the need for long-term,high-resolution monitoring to better understand their contribution to climate change mitigation.The high-resolution profiling system provided novel insights into the dynamics of NCP and CO_(2)fluxes,showing its potential as an advanced tool for studying aquaculture systems.Future research should focus on expanding the geographic and temporal scope of studies,combining observational and modeling approaches to better predict the responses of aquaculture systems to environmental changes.
基金Supported by the National Natural Science Foundation of China(No.52375037)the Outstanding Youth of Pyramid Talent Training Project of Beijing University of Civil Engineering and Architecture(No.GDRC 20220801)+1 种基金the Graduate Innovation Fund Project of Beijing University of Civil Engineering and Architecture(No.PG2025160)the Special Fund for Cultivation Projects of Beijing University of Civil Engineering and Architecture(No.X24026).
文摘An enhanced least mean square(LMS)error identification algorithm integrated with Kalman filtering is proposed to resolve accuracy degradation induced by nonlinear dynamics and parameter uncertainties in continuous rotary electro-hydraulic servo systems.This enhancement accelerates convergence and improves accuracy compared with traditional LMS.A fifth-order identification mod-el is developed based on valve-controlled hydraulic motors,with parameters identified using Kalman filter state estimation and gradient smoothing.The results indicate that the improved LMS effectively enhances parameter identification.An advanced disturbance rejection controller(ADRC)is de-signed,and its performance is compared with an optimal proportional integral derivative(PID)con-troller through Simulink simulations.The results show that the ADRC fulfills the control specifications and expands the system’s operational bandwidth.
基金Supported by National Natural Science Foundation of China(Grant Nos.52202494,52202495)Chongqing Special Project for Technological Innovation and Application Development(Grant No.CSTB2022TIAD-DEX0014).
文摘The current research of master cylinder pressure estimation mainly relies on hydraulic characteristic or vehicle dynamics.But they are not independently applicable to any environment and have their own scope of application.In addition,about the master cylinder pressure control,there are few studies that can simultaneously balance pressure building accuracy,speed,and prevent pressure overshoot and jitter.In this paper,an adaptative fusion method based on electro-hydraulic characteristic and vehicle mode is proposed to estimate the master cylinder pressure.The fusion strategy is mainly based on the prediction performance of two algorithms under different vehicle speeds,pressures,and ABS states.Apart from this,this article also includes real-time prediction of the friction model based on RLS to improve the accuracy of the electro-hydraulic mode.In order to simultaneously balance pressure control accuracy,response speed,and prevent overshoot and jitter,this article proposes an adaptative LQR controller for MC pressure control which uses fuzzy-logic controller to adjust the weights of LQR controller based on target pressure and difference compared with actual pressure.Through mode-in-loop and hardware-in-loop tests in ramp,step and sinusoidal response,the whole estimation and control system is verified based on real hydraulic system and the performance is satisfactory under these scenes.This research proposes an adaptative pressure estimation and control architecture for integrated electro-hydraulic brake system which could eliminate pressure sensors in typical scenarios and ensure the comprehensive performance of pressure control.
文摘Chronic diarrhea remains a frequent and debilitating complication among individuals living with HIV,particularly in the context of advanced immunosuppression.Its multifactorial nature often complicates both diagnosis and treatment.We present insights from our recent study investigating gut microbial alterations using 16S rRNA sequencing in HIV-positive patients with diarrheal syndrome of unclear etiology.
基金supported by Ministry of Industry and Information Technology of the People's Republic of China 2022 Industrial Technology Basic Public Service Platform Project-Traditional Chinese Medicine Whole Industry Chain Quality and Technology Service Platform(2022-230-221)Foshan Nanhai District Key Area Science and Technology Research Project[Nanke(2023)20-18].
文摘Objective:To evaluate the quality of Lygodium japonicum(Thunb.)Sw.(L.japonicum,Hai Jin Sha)by comparing its components without stewed(W)and stewed(S)using ultra-high-performance liquid chromatography(UHPLC)and chemometric analysis.Additionally,network pharmacology was employed to investigate the possible mechanisms of action of L.japonicum in the urinary calculi(UC)treatment.Methods:A fingerprinting method was established to identify components through UHPLC-tandem mass spectrometry.Chemometric techniques were used to compare the L.japonicum extraction methods.Furthermore,various network pharmacological approaches were used to identify and analyze the potential targets of the identified components in relation to UC.Results:The W and S extracts were distributed into two distinct clusters.Significant differences in the levels of protocatechuic aldehyde,caffeic acid,and p-coumaric acid were observed between S and W.Network pharmacology analysis revealed that the primary targets of L.japonicum in the UC treatment were serum albumin and epidermal growth factor receptors,with potential active components including protocatechuic acid and caffeic acid.Conclusion:This study comprehensively examined the therapeutic components of L.japonicum before and after boiling,shedding light on its potential mechanisms of action in UC treatment.These findings offer valuable insights into the development and utilization of L.japonicum resources.
基金funded by the 2023 Hospital Management Innovation Research Project by the Jiangsu Hospital Association(No.JSYGY-2-2023-551)。
文摘Objectives:This study aimed to explore the effectiveness and advantages of an“Internet+”nursing model based on user profilingin the rehabilitation of postoperative breast cancer patients.Methods:Breast cancer patients admitted to the hospital from July 2023 to September 2024 were enrolled.These patients were randomly assigned to a control group and an intervention group,with 52 patients in each group.The control group received routine nursing care,while the intervention group received an“Internet+”nursing intervention based on user profilingin addition to routine care.The intervention period lasted for one month following discharge.Before and one month after the intervention,the Fear of Progression Questionnaire-Short Form(FOP-Q-SF),the Fear of Cancer Recurrence Inventory-Short Form(FCRI-SF),Chinese Posttraumatic Growth Inventory(C-PTGI),and the Functional Assessment of Cancer Therapy-Breast(FACT-B)were applied to assess the effects of interventions.Results:A total of 104 patients were analyzed.After the intervention,FOP-Q-SF and FCRI-SF scores were significantlylower in the intervention group compared to the control group,with statistical significance(t=3.98,P<0.001;t=-7.59,P<0.001),and Cohen’s d of 0.781 and 1.49,respectively.Additionally,CPTGI and FACT-B scores in the intervention group were significantly higher than those in the control group(t=-6.534,P<0.001;t=-4.579,P<0.001),with Cohen’s d of 0.585 and 0.656.Conclusions:An“Internet+”nursing model based on user profilingcould reduce postoperative breast cancer patients fear of disease progression and cancer recurrence,also enhancing posttraumatic growth and overall quality of life.
