South African indigenous bucks are raised under extensive conditions and are more likely to reject artificial vagina (AV) due to their limited contact with human beings, as they are less handled. The purpose of the st...South African indigenous bucks are raised under extensive conditions and are more likely to reject artificial vagina (AV) due to their limited contact with human beings, as they are less handled. The purpose of the study was to compare goat semen collection techniques used for goats based on semen traits of South African indigenous bucks during the natural breeding season. A total of eight South African indigenous bucks were used, four bucks per semen collection technique due to their scarcity. Before semen collection with AV group, ten bucks were trained and exposed to AV. Semen was then collected from both groups over a period of six weeks per individual buck. Semen volume, pH and sperm concentration were evaluated immediately. The Computer Aided Sperm Analysis (CASA) assessed the sperm motility rates. Sperm morphology was evaluated using a fluorescence microscope with the aid of eosin-nigrosin staining. Analysis of variance was used to test the differences among the semen collection groups following assessment by CASA. Semen collected with EE resulted in higher semen volume (1.1 mL) compared with AV (0.5 mL) technique. However, AV technique resulted in higher total sperm motility and rapid movement (91.9% and 48.7%), live sperm (72%) and sperm concentration (635.6 × 106 sperm/mL) compared with EE (86.3% and 30.4%;69% and 463.7 × 106 sperm/mL, respectively). There were no significant differences observed in pH and sperm morphology characteristics between two semen collection techniques. Moreover, EE had higher sperm moving in a progressive (53.6%) and medium rate compared with AV (41.1% and 21.9%) technique. It was concluded that the AV yielded higher total sperm motility rate, sperm concentration and rapid sperm movement compared with EE. Therefore, AV was more suitable for semen collection of South African indigenous bucks, even though training was problematic to such indigenous bucks.展开更多
Objective:To evaluate the effects of semen collection methods and different cooling rates on cooled and frozen-thawed Saanen goat semen.Methods:Twenty bucks were divided into two groups of 10 animals per group based o...Objective:To evaluate the effects of semen collection methods and different cooling rates on cooled and frozen-thawed Saanen goat semen.Methods:Twenty bucks were divided into two groups of 10 animals per group based on semen collection methods:artificial vagina and electro-ejaculator.Samples from each collection method were pooled and diluted with Tris-based extender.The pooled semen was divided into two cooling methods:slow cooling and fast cooling.Sperm motility and velocity,sperm plasma membrane integrity,sperm viability,mitochondrial membrane potential and DNA integrity were evaluated before dilution,immediately after cooling at 4℃and at 24 h after freezing by using computer assisted sperm analyser method and epifluorescence microscope.Results:Mean values for total motility,rapid-speed and progressive motile spermatozoa were significantly higher in semen collected with artificial vagina than electro-ejaculator(P<0.001).Slow cooling resulted in higher percentages of total motile,rapid-speed and progressive motile spermatozoa as compared to fast cooling(P<0.05).The post-thaw percentage of rapid-speed spermatozoa collected by artificial vagina in slow cooled sperm was significantly higher as compared to electro-ejaculator method(P<0.05).The mean values of curvilinear velocity and straight-line velocity were significantly higher in slow cooled semen obtained by artificial vagina(P<0.001).Similarly,the combination of artificial vagina and slow cooling resulted in significantly higher post-thaw percentages of straight-line velocity,average-path velocity,sperm plasma membrane integrity,viability and acrosome integrity(P<0.01).No significant differences were observed in slow cooling between artificial vagina and electro-ejaculator in terms of post-thaw sperm mitochondrial membrane potential and DNA integrity(P>0.05).Conclusions:The use of artificial vagina and slow cooling rate in cryopreservation protocol improves the cooled and post-thaw sperm quality of Saanen buck semen.展开更多
文摘South African indigenous bucks are raised under extensive conditions and are more likely to reject artificial vagina (AV) due to their limited contact with human beings, as they are less handled. The purpose of the study was to compare goat semen collection techniques used for goats based on semen traits of South African indigenous bucks during the natural breeding season. A total of eight South African indigenous bucks were used, four bucks per semen collection technique due to their scarcity. Before semen collection with AV group, ten bucks were trained and exposed to AV. Semen was then collected from both groups over a period of six weeks per individual buck. Semen volume, pH and sperm concentration were evaluated immediately. The Computer Aided Sperm Analysis (CASA) assessed the sperm motility rates. Sperm morphology was evaluated using a fluorescence microscope with the aid of eosin-nigrosin staining. Analysis of variance was used to test the differences among the semen collection groups following assessment by CASA. Semen collected with EE resulted in higher semen volume (1.1 mL) compared with AV (0.5 mL) technique. However, AV technique resulted in higher total sperm motility and rapid movement (91.9% and 48.7%), live sperm (72%) and sperm concentration (635.6 × 106 sperm/mL) compared with EE (86.3% and 30.4%;69% and 463.7 × 106 sperm/mL, respectively). There were no significant differences observed in pH and sperm morphology characteristics between two semen collection techniques. Moreover, EE had higher sperm moving in a progressive (53.6%) and medium rate compared with AV (41.1% and 21.9%) technique. It was concluded that the AV yielded higher total sperm motility rate, sperm concentration and rapid sperm movement compared with EE. Therefore, AV was more suitable for semen collection of South African indigenous bucks, even though training was problematic to such indigenous bucks.
文摘Objective:To evaluate the effects of semen collection methods and different cooling rates on cooled and frozen-thawed Saanen goat semen.Methods:Twenty bucks were divided into two groups of 10 animals per group based on semen collection methods:artificial vagina and electro-ejaculator.Samples from each collection method were pooled and diluted with Tris-based extender.The pooled semen was divided into two cooling methods:slow cooling and fast cooling.Sperm motility and velocity,sperm plasma membrane integrity,sperm viability,mitochondrial membrane potential and DNA integrity were evaluated before dilution,immediately after cooling at 4℃and at 24 h after freezing by using computer assisted sperm analyser method and epifluorescence microscope.Results:Mean values for total motility,rapid-speed and progressive motile spermatozoa were significantly higher in semen collected with artificial vagina than electro-ejaculator(P<0.001).Slow cooling resulted in higher percentages of total motile,rapid-speed and progressive motile spermatozoa as compared to fast cooling(P<0.05).The post-thaw percentage of rapid-speed spermatozoa collected by artificial vagina in slow cooled sperm was significantly higher as compared to electro-ejaculator method(P<0.05).The mean values of curvilinear velocity and straight-line velocity were significantly higher in slow cooled semen obtained by artificial vagina(P<0.001).Similarly,the combination of artificial vagina and slow cooling resulted in significantly higher post-thaw percentages of straight-line velocity,average-path velocity,sperm plasma membrane integrity,viability and acrosome integrity(P<0.01).No significant differences were observed in slow cooling between artificial vagina and electro-ejaculator in terms of post-thaw sperm mitochondrial membrane potential and DNA integrity(P>0.05).Conclusions:The use of artificial vagina and slow cooling rate in cryopreservation protocol improves the cooled and post-thaw sperm quality of Saanen buck semen.
