期刊文献+
共找到1,165篇文章
< 1 2 59 >
每页显示 20 50 100
Construction of Constant Rank and Orbit Codes over Finite Chain Rings
1
作者 GUO Ye LIU Xiusheng 《Wuhan University Journal of Natural Sciences》 2025年第3期289-301,共13页
In this paper,we first generalize the constant dimension and orbit codes over finite fields to the constant rank and orbit codes over finite chain rings.Then we provide a relationship between constant rank codes over ... In this paper,we first generalize the constant dimension and orbit codes over finite fields to the constant rank and orbit codes over finite chain rings.Then we provide a relationship between constant rank codes over finite chain rings and constant dimension codes over the residue fields.In particular,we prove that an orbit submodule code over a finite chain ring is a constant rank code.Finally,for special finite chain ring F_(q)+γF_(q),we define a Gray mapφfrom(F_(q)+γF_(q))^(n)to F^(2n)_(q),and by using cyclic codes over F_(q)+γF_(q),we obtain a method of constructing an optimum distance constant dimension code over F_(q). 展开更多
关键词 finite chain ring rank of linear codes constant rank codes orbit codes
原文传递
Surface reconstruction of complex contour lines based on chain code matching technique 被引量:1
2
作者 姜晓彤 《Journal of Southeast University(English Edition)》 EI CAS 2005年第4期432-435,共4页
A new method for solving the tiling problem of surface reconstruction is proposed. The proposed method uses a snake algorithm to segment the original images, the contours are then transformed into strings by Freeman'... A new method for solving the tiling problem of surface reconstruction is proposed. The proposed method uses a snake algorithm to segment the original images, the contours are then transformed into strings by Freeman' s code. Symbolic string matching technique is applied to establish a correspondence between the two consecutive contours. The surface is composed of the pieces reconstructed from the correspondence points. Experimental results show that the proposed method exhibits a good behavior for the quality of surface reconstruction and its time complexity is proportional to mn where m and n are the numbers of vertices of the two consecutive slices, respectively. 展开更多
关键词 chain code string matching surface reconstruction local shape feature
在线阅读 下载PDF
Multiple fault diagnosis of down-hole conditions of sucker-rod pumping wells based on Freeman chain code and DCA 被引量:13
3
作者 LI Kun GAO Xian-wen +2 位作者 YANG Wei-bing DAI Ying-long TIAN Zhong-da 《Petroleum Science》 SCIE CAS CSCD 2013年第3期347-360,共14页
It is important to achieve continuous, stable and efficient pumping well operation in actual oilfield operation. Down-hole pumping well working conditions can be monitored in real-time and a reasonable production sche... It is important to achieve continuous, stable and efficient pumping well operation in actual oilfield operation. Down-hole pumping well working conditions can be monitored in real-time and a reasonable production scheme can be designed when computer diagnosis is used. However, it is difficult to make a comprehensive analysis to supply efficient technical guidance for operation of the pumping well with multiple faults of down-hole conditions, which cannot be effectively dealt with by the common methods. To solve this problem, a method based on designated component analysis (DCA) is used in this paper. Freeman chain code is used to represent the down-hole dynamometer card whose important characteristics are extracted to construct a designated mode set. A control chart is used as a basis for fault detection. The upper and lower control lines on the control chart are determined from standard samples in normal working conditions. In an incompletely orthogonal mode, the designated mode set could be divided into some subsets in which the modes are completely orthogonal. The observed data is projected into each designated mode to realize fault detection according to the upper and lower control lines. The examples show that the proposed method can effectively diagnose multiple faults of down-hole conditions. 展开更多
关键词 Sucker-rod pumping wells multiple faults designated component analysis control chart Freeman chain code dynamometer card
原文传递
Self-Dual Permutation Codes over Finite Chain Rings 被引量:1
4
作者 YUAN Yuan ZHANG Huanguo 《Wuhan University Journal of Natural Sciences》 CAS 2007年第6期992-996,共5页
Permutation codes over finite chain rings are introduced; by using the character of the finite chain rings and the knowledge of representation of group, some conditions for existence or non-existence of self-dual perm... Permutation codes over finite chain rings are introduced; by using the character of the finite chain rings and the knowledge of representation of group, some conditions for existence or non-existence of self-dual permutation codes over finite chain rings are obtained. Specially, when the group is a direct product of a 2-group and a T-group, and the group action is transitive, the sufficient and necessary condition of the existence of permutation codes is given. 展开更多
关键词 finite chain ring group code permutation code self-dual code
在线阅读 下载PDF
Face Recognition Using Chain Codes
5
作者 Nazmeen B. Boodoo-Jahangeer Sunilduth Baichoo 《Journal of Signal and Information Processing》 2013年第3期154-157,共4页
Face recognition is an active area of biometrics. This study investigates the use of Chain Codes as features for recognition purpose. Firstly a segmentation method, based on skin color model was applied, followed by c... Face recognition is an active area of biometrics. This study investigates the use of Chain Codes as features for recognition purpose. Firstly a segmentation method, based on skin color model was applied, followed by contour detection, then the chain codes of the contours were determined. The first difference of chain codes were calculated since the latter is invariant to rotation. The features were calculated and stored in a matrix. Experiments were performed using the University of Essex Face database, and results show a recognition rate of 95% with this method, when compared with Principal Components Analysis (PCA) giving 87.5% recognition rate. 展开更多
关键词 FACE RECOGNITION BIOMETRICS chain codeS PCA
在线阅读 下载PDF
Auto-selection order of Markov chain for background sequences with chi-square test
6
作者 谢雪英 孙啸 陆祖宏 《Journal of Southeast University(English Edition)》 EI CAS 2003年第4期311-316,共6页
Modeling non coding background sequences appropriately is important for the detection of regulatory elements from DNA sequences. Based on the chi square statistic test, some explanations about why to choose higher ... Modeling non coding background sequences appropriately is important for the detection of regulatory elements from DNA sequences. Based on the chi square statistic test, some explanations about why to choose higher order Markov chain model and how to automatically select the proper order are given in this paper. The chi square test is first run on synthetic data sets to show that it can efficiently find the proper order of Markov chain. Using chi square test, distinct higher order context dependences inherent in ten sets of sequences of yeast S.cerevisiae from other literature have been found. So the Markov chain with higher order would be more suitable for modeling the non coding background sequences than an independent model. 展开更多
关键词 non coding sequences regulatory elements chi square test Markov chain
在线阅读 下载PDF
CYCLIC AND NEGACYCLIC CODES OF LENGTH 2p^s OVER F_(p^m) + uF_(p^m) 被引量:2
7
作者 刘修生 许小芳 《Acta Mathematica Scientia》 SCIE CSCD 2014年第3期829-839,共11页
In this article, we focus on cyclic and negacyclic codes of length 2p^s over the ring R = Fp^m + uFp^m, where p is an odd prime. On the basis of the works of Dinh (in J.Algebra 324,940-950,2010), we use the Chinese... In this article, we focus on cyclic and negacyclic codes of length 2p^s over the ring R = Fp^m + uFp^m, where p is an odd prime. On the basis of the works of Dinh (in J.Algebra 324,940-950,2010), we use the Chinese Remainder Theorem to establish the algebraic structure of cyclic and negacyclic codes of length 2p^s over the ring Fp^m + uFp^m in terms of polynomial generators. Furthermore, we obtain the number of codewords in each of those cyclic and negacyclic codes. 展开更多
关键词 Negacyclic codes cyclic codes repeated-root codes finite chain ring finite local ring
在线阅读 下载PDF
CYCLIC CODES OVER FORMAL POWER SERIES RINGS 被引量:1
8
作者 Dougherty Steven T. 刘宏伟 《Acta Mathematica Scientia》 SCIE CSCD 2011年第1期331-343,共13页
In this article, cyclic codes and negacyclic codes over formal power series rings are studied. The structure of cyclic codes over this class of rings is given, and the relationship between these codes and cyclic codes... In this article, cyclic codes and negacyclic codes over formal power series rings are studied. The structure of cyclic codes over this class of rings is given, and the relationship between these codes and cyclic codes over finite chain rings is obtained. Using an isomorphism between cyclic and negacyclic codes over formal power series rings, the structure of negacyclic codes over the formal power series rings is obtained. 展开更多
关键词 Finite chain rings cyclic codes negacyclic codes γ-adic codes
在线阅读 下载PDF
Adding Sub-chain Method for Structural Synthesis of Planar Closed Kinematic Chains 被引量:2
9
作者 DENG Zongquan YANG Fei TAO Jianguo 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2012年第2期206-213,共8页
For at least the past five decades,structural synthesis has been used as a main means of finding better mechanisms with some predefined function.In structural synthesis,isomorphism identification is still a problem un... For at least the past five decades,structural synthesis has been used as a main means of finding better mechanisms with some predefined function.In structural synthesis,isomorphism identification is still a problem unsolved well,and to solve this problem is very significant to the design of new mechanisms.According to the given degree of freedom(DOF) and link connection property of planar closed chain mechanisms,vertex assortment is obtained.For solving the isomorphism problem,a method of the adding sub-chains is proposed with the detailed steps and algorithms in the synthesizing process.Employing this method,the identification code and formation code of every topological structure are achieved,therefore many isomorphic structures could be eliminated in time during structural synthesis by comparing those codes among different topological graphs,resulting in the improvement of synthesizing efficiency and accuracy,and the approach for eliminating rigid sub-chains in and after the synthesizing process is also presented.Some examples are given,including how to add sub-chains,how to detect simple rigid sub-chains and how to obtain identification codes and formulation codes et al.Using the adding sub-chain method,the relative information of some common topological graphs is given in the form of table.The comparison result is coincident with many literatures,so the correctness of the adding sub-chain method is convinced.This method will greatly improve the synthesizing efficiency and accuracy,and has a good potential for application. 展开更多
关键词 structural synthesis adding sub-chain method identification code formation code rigid sub-chains
在线阅读 下载PDF
ON THE CHARACTERIZATION OF CYCLIC CODES OVER TWO CLASSES OF RINGS
10
作者 刘修生 《Acta Mathematica Scientia》 SCIE CSCD 2013年第2期413-422,共10页
Let R be a finite chain ring with maximal ideal (7) and residue field F,and letγ be of nilpotency index t. To every code C of length n over R, a tower of codes C = (C : γ0) C_ (C: 7) C ... C_ (C: γ2) C_ ... Let R be a finite chain ring with maximal ideal (7) and residue field F,and letγ be of nilpotency index t. To every code C of length n over R, a tower of codes C = (C : γ0) C_ (C: 7) C ... C_ (C: γ2) C_ .-. C_ (C:γ^t-1) can be associated with C, where for any r C R, (C : r) = {e C Rn I re E C}. Using generator elements of the projection of such a tower of codes to the residue field F, we characterize cyclic codes over R. This characterization turns the condition for codes over R to be cyclic into one for codes over the residue field F. Furthermore, we obtain a characterization of cyclic codes over the formal power series ring of a finite chain ring. 展开更多
关键词 Finite chain rings formal power series rings cyclic codes tower of codes Hensel lift
在线阅读 下载PDF
Network Coding for Wireless Sensor Network Cluster over Rayleigh Fading Channel: Finite State Markov Chain
11
作者 Mohammad Alhihi 《International Journal of Communications, Network and System Sciences》 2017年第1期1-11,共11页
Network Coding (NC) is confirmed to be power and bandwidth efficient technique, because of the less number of transmitted packets over the network. Wireless Sensor Network (WSN) is usually power limited network applic... Network Coding (NC) is confirmed to be power and bandwidth efficient technique, because of the less number of transmitted packets over the network. Wireless Sensor Network (WSN) is usually power limited network application, and in many scenarios it is power and bandwidth limited application. The proposed scenario in this paper applies the advantages of NC over WSN to obtain such power and bandwidth efficient WSN. To take the advantages of NC over the one of the most needed applications i.e., WSN, we come up to what this paper is discussing. We consider a WSN (or its cluster) that consists of M nodes that transmit equal-length information packets to a common destination node D over wireless Rayleigh block-fading channel where the instantaneous SNR is assumed to be constant over a single packet transmission period. Finite-State packet level Markov chain (FSMC) model is applied to give the channel more practical aspect. The simulation results showed that applying NC over the WSN cluster improved the channel bandwidth significantly by decreasing the number of the Automatic Repeat Request (ARQ), resulting in improving the power consumption significantly. The results are collected for different transmission distances to evaluate the behavior to the proposed scenario with regard to the bath losses effect. 展开更多
关键词 RAYLEIGH FADING Channel Network Coding Finite-Stage MARKOV chain
在线阅读 下载PDF
Research progress of long-chain non coding RNA malat1 in cervical cancer
12
作者 Wen Zhou Yue Xia +2 位作者 Bi-Bo Tan Chang-Xian Chen Ling-Zhao Mo 《Journal of Hainan Medical University》 2019年第24期72-75,共4页
Objective:Long non coding RNA (lncrna) is a kind of RNA with a length of more than 200 bp but not directly encoding protein. It is found that lncrna involves many important biological processes, such as cell prolifera... Objective:Long non coding RNA (lncrna) is a kind of RNA with a length of more than 200 bp but not directly encoding protein. It is found that lncrna involves many important biological processes, such as cell proliferation, cell survival, cell differentiation, organogenesis, genomic imprinting, and chromatin remodeling. The metastasis associated lung cancer transcript 1 (malat1) is an important member of the lncrna family. It is located on human chromosome 11 (11p13.1), with a length of 8000 BP. It is an intergenic transcript. Malat1 is highly expressed in cervical cancer, which may be closely related to the occurrence and development of cervical cancer, and may become a new target for diagnosis and treatment of cervical cancer. This article reviews the research status of the relationship between lncrna malat1 and cervical cancer. 展开更多
关键词 long chain non CODING RNA malat1 CERVICAL cancer
暂未订购
Construction of Self-dual Codes over F_p+vF_p
13
作者 ZHANG Guang-hui WANG Dong-qing 《Chinese Quarterly Journal of Mathematics》 2018年第4期358-368,共11页
In this paper, we give an explicit construction for self-dual codes over F_p+vF_p(v^2= v) and determine all the self-dual codes over F_p+ vF_p by using self-dual codes over finite field F_p, where p is a prime.
关键词 Linear code SELF-DUAL code codes OVER Fp +vFp non-chain ring
在线阅读 下载PDF
熊果酸对甲状腺乳头状癌细胞LINC01089及其靶基因表达的影响 被引量:1
14
作者 伊琳 何亚丽 +3 位作者 吕红英 王晓科 杨鑫 顾远晖 《中华中医药学刊》 北大核心 2025年第9期11-15,I0001-I0004,共9页
目的分析熊果酸对甲状腺乳头状癌(Papillary thyroid carcinoma,PTC)长链非编码RNA(Long chain non coding RNA,lncRNA)-LINC01089及其靶基因表达的影响。方法LINC01089为前期通过转录组测序筛选并通过验证的差异表达lncRNA,体外常规培... 目的分析熊果酸对甲状腺乳头状癌(Papillary thyroid carcinoma,PTC)长链非编码RNA(Long chain non coding RNA,lncRNA)-LINC01089及其靶基因表达的影响。方法LINC01089为前期通过转录组测序筛选并通过验证的差异表达lncRNA,体外常规培养甲状腺乳头状癌TPC-1细胞、正常人甲状腺细胞(Nthy-Ori3-1)。将细胞分为正常组(正常人甲状腺细胞组)、对照组(TPC-1细胞组)、不同浓度熊果酸(Ursolic acid,UA)干预组(10、20、40μmol·L^(-1))。CCK-8法检测各组甲状腺乳头状癌TPC-1细胞的存活率;划痕实验、Transwell实验检测不同浓度UA对TPC-1细胞水平、垂直迁移以及侵袭的影响作用;利用RT-qPCR法检测TPC-1细胞中LINC01089的相对表达量及熊果酸的干预作用;RT-qPCR及Western blot检测靶基因丝状伪足相关基因(Ras homolog family member F,RHOF)、4-羟基苯丙酮酸双加氧酶(4-Hydroxyphenylpyruvate dioxygenase,HPD)的mRNA及蛋白表达水平以及熊果酸的干预作用。结果不同浓度的UA(10、20、40、80μmol·L^(-1))作用不同时间(12~48 h)后均能抑制TPC-1细胞的增殖,当UA浓度为10~40μmol·L^(-1)时,在12~24 h内随着浓度的增加和作用时间的延长,细胞的抑制效应逐渐增强,差异具有统计学意义(P<0.05),UA处理TPC-1细胞24 h后,细胞增殖缓慢,随着UA(10、20、40μmol·L^(-1))浓度的增高,细胞出现凋亡的形态学改变;与对照组相比,UA(10、20、40μmol·L^(-1))组可抑制TPC-1细胞迁移和侵袭,呈明显浓度依赖性,差异具有统计学意义(P<0.01)。熊果酸能不同程度上调LINC01089的表达量,下调RHOF、HPD的mRNA及蛋白表达水平。结论熊果酸对甲状腺乳头状癌细胞具有一定的抑制作用,其机制可能通过上调LINC01089作用于靶基因RHOF发挥抑癌效应。 展开更多
关键词 长链非编码RNA 甲状腺乳头状癌 熊果酸 基因
原文传递
一种基于链码的农村公路线形比对与筛选算法 被引量:2
15
作者 范文涛 孙翠羽 +2 位作者 崔应寿 刘柳杨 龙佳宁 《交通运输研究》 2025年第1期102-110,共9页
为提升农村公路电子地图数据校核的效率和精确性,提出一种基于链码技术的农村公路线形比对与筛选算法模型。首先,引入链码技术对农村公路电子地图中的路线进行识别和提取,获得路线的起点、拐点及方向变化等线形特征信息,并实现不同地理... 为提升农村公路电子地图数据校核的效率和精确性,提出一种基于链码技术的农村公路线形比对与筛选算法模型。首先,引入链码技术对农村公路电子地图中的路线进行识别和提取,获得路线的起点、拐点及方向变化等线形特征信息,并实现不同地理区域和时间维度下的路线线形链码特征信息的精确匹配。其次,选取我国东部、中部、西部区域中典型区县的2022及2023年农村公路线形数据,对算法模型进行训练,得出路线线形链码差异的不同参数阈值;对于超出阈值的农村公路路线数据,再运用卷积神经网络算法从遥感影像中提取路线线形并获取线形链码特征信息进行比对筛选以及影像评价。最后,将该算法应用于2023年度全国农村公路电子地图中459.9万km的路线数据进行解算验证。结果表明,与传统“全重叠”方法相比,该算法效率提升了72.1%,识别率从64.5%提升至90.6%,准确率从95.7%提升到97.3%。研究证明,该算法显著提高了电子地图线形数据处理的效率和准确率,可为农村公路基础数据入库提供技术支撑,提升农村公路数字化发展水平。 展开更多
关键词 链码算法 线形比对 农村公路 遥感影像 卷积神经网络
在线阅读 下载PDF
从LncRNA Meg3调控细胞自噬途径探讨肝豆灵治疗Wilson病肝纤维化的作用机制 被引量:1
16
作者 汪瀚 花代平 +3 位作者 殷馨 宣巧玉 孙兰婷 杨文明 《中华中医药杂志》 北大核心 2025年第5期2574-2579,共6页
目的:从长链非编码RAN(LncRNA)母系表达基因3(Meg3)调控磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白(PI3K/Akt/mTOR)信号通路探讨肝豆灵(GDL)对Wilson病(WD)肝纤维化的作用机制。方法:将SD大鼠随机分为对照组,模型组,GDL低、中... 目的:从长链非编码RAN(LncRNA)母系表达基因3(Meg3)调控磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白(PI3K/Akt/mTOR)信号通路探讨肝豆灵(GDL)对Wilson病(WD)肝纤维化的作用机制。方法:将SD大鼠随机分为对照组,模型组,GDL低、中、高剂量(GDL-L、GDL-M、GDL-H)组及青霉胺(PCA)组,每组10只。使用五水合硫酸铜灌胃构建铜负荷WD模型。后采用HE和Masson染色观察肝纤维化组织病理学改变;检测血清谷草转氨酶(AST)、谷丙转氨酶(ALT)水平;ELISA法检测肝纤四项指标。透射电镜观察肝组织超微结构;免疫荧光观察自噬相关蛋白(Beclin-1)、微管结合蛋白1轻链3-Ⅱ(LC3-Ⅱ)蛋白表达;Western Blot和RT-qPCR分别检测α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原蛋白(Collagen Ⅰ)、Beclin-1、LC3-Ⅱ、PI3K、Akt、mTOR相关蛋白和基因表达;RT-qPCR检测LncRNA Meg3表达。结果:与对照组比较,模型组肝组织细胞水肿及坏死,伴有炎症细胞浸润,可见胶原纤维沉积。与模型组比较,GDL各剂量组ALT、AST、肝纤四项指标显著下降(P<0.01,P<0.05),α-SMA、Collagen Ⅰ、Beclin-1、LC3-Ⅱ/LC3-Ⅰ蛋白表达水平显著下降(P<0.01,P<0.05);p-PI3K/PI3K、p-Akt/Akt、p-mTOR/mTOR蛋白表达显著升高(GDL-L组p-PI3K/PI3K除外)(P<0.05,P<0.01);α-SMA、Collagen Ⅰ、Beclin-1基因表达显著下降(P<0.01),LncRNA Meg3、PI3K、Akt、mTOR基因表达显著升高(GDL-L组PI3K、Akt除外)(P<0.01)。结论:GDL可能通过上调LncRNA Meg3的表达,激活PI3K/Akt/mTOR信号通路抑制自噬,改善肝纤维化。 展开更多
关键词 肝豆灵 长链非编码RAN 母系表达基因3 PI3K/Akt/mTOR信号通路 自噬 肝纤维化 WILSON病 机制
原文传递
LncRNA FGD5-AS1通过miR-103a-3p/RNF38轴影响结肠癌细胞的恶性生物学行为 被引量:1
17
作者 石沛 庞雪莹 +4 位作者 邓升华 祝金华 赵士梅 马秀岩 马静 《河北医学》 2025年第1期15-21,共7页
目的:探讨长链非编码RNA FGD5反义RNA1(LncRNA FGD5-AS1)靶向调控miR-103a-3p/环指蛋白38(RNF38)轴对结肠癌细胞恶性生物学行为的影响。方法:使用荧光定量PCR检测LncRNA FGD5-AS1、miR-103a-3p在人结肠黏膜上皮细胞和人结肠癌细胞SW480... 目的:探讨长链非编码RNA FGD5反义RNA1(LncRNA FGD5-AS1)靶向调控miR-103a-3p/环指蛋白38(RNF38)轴对结肠癌细胞恶性生物学行为的影响。方法:使用荧光定量PCR检测LncRNA FGD5-AS1、miR-103a-3p在人结肠黏膜上皮细胞和人结肠癌细胞SW480中的表达水平;通过StarBase和TargetScan数据库分析LncRNA FGD5-AS1、miR-103a-3p、RNF38之间的结合位点,使用双荧光酶报告基因检测进一步验证;将SW480细胞随机分为对照(Control)组、pcDNA-NC组、pcDNA-FGD5-AS1组、si-NC组、si-FGD5-AS1组、si-FGD5-AS1+inhibitor NC组、si-FGD5-AS1+miR-103a-3p inhibitor组,通过CCK-8、流式细胞术、Transwell试验、荧光定量PCR及Western blot法检测细胞增殖、凋亡、侵袭、FGD5-AS1、miR-103a-3p水平及RNF3蛋白表达水平。结果:与人结肠黏膜上皮细胞相比,SW480细胞中LncRNA FGD5-AS1表达水平显著降低(1.00±0.00比0.48±0.10,t=12.737,P<0.05),miR-103a-3p表达水平显著升高(1.00±0.00比1.61±0.18,t=8.301,P<0.05);LncRNA FGD5-AS1可靶向负调控miR-103a-3p表达(P<0.05);miR-103a-3p可靶向正调控RNF38表达(P<0.05);与pcDNA-NC组相比,pcDNA-FGD5-AS1组SW480细胞中FGD5-AS1水平升高(0.98±0.10比2.34±0.45,t=7.227,P<0.05),细胞凋亡率增加(8.64±1.32比16.21±2.75,t=6.079,P<0.05),而miR-103a-3p表达水平(1.01±0.12比0.42±0.07,t=10.403,P<0.05)、RNF38蛋白表达水平(0.59±0.09比0.32±0.17,t=3.438,P=0.006)、细胞增殖活性(0.63±0.09比0.34±0.06,t=6.567,P<0.05)、细胞侵袭能力(112.63±14.94比43.82±5.67,t=10.548,P<0.05)降低。si-FGD5-AS1组细胞变化趋势与pcDNA-FGD5-AS1组相反,且miR-103a-3p inhibitor可逆转si-FGD5-AS1组的变化(P<0.05)。结论:干扰LncRNA FGD5-AS1可能通过靶向上调miR-103a-3p,促进RNF38蛋白表达,从而提高结肠癌细胞增殖和侵袭能力,降低结肠癌细胞凋亡率,而上调LncRNA FGD5-AS1可抑制结肠癌细胞的恶性生物学行为。 展开更多
关键词 结肠癌细胞 长链非编码RNA FGD5反义RNA1 微小RNA-103a-3p 环指蛋白38
暂未订购
LncRNA SNHG14调控miR-223-3p减轻大鼠脓毒症相关性肺损伤的机制探讨 被引量:1
18
作者 李莹 肖文彪 林晓 《中国呼吸与危重监护杂志》 2025年第1期18-25,共8页
目的探讨长链非编码核糖核酸核内小RNA宿主基因14(long chain non-coding ribonucleic acid small nucleolar RNA host gene 14,LncRNA SNHG14)调控微小核糖核酸223-3p(microribonucleic acid-223-3p,miR-223-3p)减轻大鼠脓毒症相关性... 目的探讨长链非编码核糖核酸核内小RNA宿主基因14(long chain non-coding ribonucleic acid small nucleolar RNA host gene 14,LncRNA SNHG14)调控微小核糖核酸223-3p(microribonucleic acid-223-3p,miR-223-3p)减轻大鼠脓毒症相关性肺损伤的作用。方法建立脓毒症大鼠模型,将大鼠随机分为SNHG14上调组、SNHG14上调对照组、SNHG14下调组、SNHG14下调对照组、miR-223-3p上调组、miR-223-3p上调对照组、miR-223-3p下调组、miR-223-3p下调对照组、模型组,另设假手术组。均经尾静脉给药,48 h后处死,取肺组织检测LncRNA SNHG14、miR-223-3p表达;检测大鼠血清肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素(interleukin,IL)-1β、L-18水平、肺组织湿/干重比值(wet weight/dry weight,W/D)、肺泡液体清除率(alveolar fluid clearance rate,AFC);观察肺组织病理改变;检测肺组织人NOD样受体家族蛋白3(NOD like receptor family protein 3,NLPR3)、半胱氨酸天冬氨酸蛋白酶1(cysteine-requiring aspartate protease 1,Caspase-1)、1-氨基环丙烷-1-羧酸合酶(1-aminocyclopropane-1-carboxylate synthase,ACS)基因和蛋白表达;双荧光素酶报告基因实验验证LncRNA SNHG14对miR-223-3p的靶向调控作用。结果与假手术组比较,模型组肺组织LncRNA SNHG14表达、血清炎症因子水平、W/D、病理改变量化评分、肺组织NLPR3、Caspase-1、ACS表达均升高(P<0.05),而miR-223-3p表达、AFC均下降(P<0.05)。与模型组和相应对照组比较,SNHG14上调组LncRNA SNHG14表达升高(P<0.05),且SNHG14上调组与miR-223-3p下调组miR-223-3p表达、AFC均下降(P<0.05),血清炎症因子水平、W/D、病理改变量化评分及肺组织NLPR3、Caspase-1、ACS表达均升高(P<0.05)。与模型组和相应对照组比较,SNHG14下调组LncRNA SNHG14表达下降(P<0.05),SNHG14下调组与miR-223-3p上调组miR-223-3p表达、AFC均增加(P<0.05),血清炎症因子水平、W/D、病理改变量化评分及肺组织NLPR3、Caspase-1、ACS表达均下降(P<0.05)。LncRNA SNHG14与miR-223-3p存在结合位点,且前者可负反馈靶向调控后者。结论下调LncRNA SNHG14靶向增加了miR-223-3p表达、并抑制了NLRP3通路减轻脓毒症相关性肺损伤,与抑制炎症反应有关;而上调LncRNA SNHG14则负反馈靶向了miR-223-3p、激活了NLRP3通路加重脓毒症相关性肺损伤。 展开更多
关键词 脓毒症 肺损伤 长链非编码核糖核酸 核内小RNA宿主基因14 微小核糖核酸-223-3p
原文传递
基于思维代码的大语言模型多工具协调调用提示方法
19
作者 江志英 黄震宇 +6 位作者 宋晨炜 张泽宇 韩众和 谷伟伟 龚启航 刘思叶 赵妍 《北京化工大学学报(自然科学版)》 北大核心 2025年第3期105-113,共9页
大语言模型因其出色的理解和生成能力被广泛应用于自动化智能助手的开发。然而,它们在处理复杂问题时,常因训练数据庞杂等局限性而难以调用正确的工具和生成准确的函数名称及参数。为了提高在对复杂问题进行任务规划时模型生成工具调用... 大语言模型因其出色的理解和生成能力被广泛应用于自动化智能助手的开发。然而,它们在处理复杂问题时,常因训练数据庞杂等局限性而难以调用正确的工具和生成准确的函数名称及参数。为了提高在对复杂问题进行任务规划时模型生成工具调用的准确性,提出一种基于思维链的提示方法—思维代码(Reasoning to Annotation and Coding,ReACo),充分利用预训练的数据,通过代码和注释结合的任务规划提示方式增强语言模型对复杂任务的理解能力,并基于此提出一种新的大语言模型思维提示框架ReACoGPT。基于ReACoGPT提示的语言模型能够准确调用多个插件,依据事实提供富有逻辑的任务规划能力,从而在保持任务规划逻辑性的同时准确使用需求的真实数据。实验结果表明,相较于现有方法,ReACoGPT在RestBench数据集上的正确率、正确路径率及解决长度等多项指标得到提高,证实了ReACo提示方式能够增强大语言模型的规划和推理能力,有效利用大量训练数据对任务进行有效规划,促进了大语言模型在工具学习方面的进一步发展。 展开更多
关键词 思维代码(ReACo) 工具调用 思维链 大语言模型
在线阅读 下载PDF
LncRNA H19靶向miR-766-3p对缺氧复氧诱导的大鼠心肌细胞株H9c2凋亡的影响
20
作者 邢智 李辉 +3 位作者 邱海棠 迪丽胡玛尔·阿布来提 朱梦柏 高颖 《中西医结合心脑血管病杂志》 2025年第16期2461-2467,共7页
目的:观察长链非编码核糖核酸(Lnc RNA)H19对缺氧复氧诱导的大鼠心肌细胞株H9c2凋亡的影响,并探讨其对微小核糖核酸(miR-766-3p)的靶向调控作用。方法:取大鼠心肌细胞株H9c2培养、分组。除空白组和正常组外,Lnc RNA H19上调组、Lnc RNA ... 目的:观察长链非编码核糖核酸(Lnc RNA)H19对缺氧复氧诱导的大鼠心肌细胞株H9c2凋亡的影响,并探讨其对微小核糖核酸(miR-766-3p)的靶向调控作用。方法:取大鼠心肌细胞株H9c2培养、分组。除空白组和正常组外,Lnc RNA H19上调组、Lnc RNA H19上调对照组、Lnc RNA H19下调组、Lnc RNA H19下调对照组、miR-766-3p上调组、miR-766-3p上调对照组、miR-766-3p下调组、miR-766-3p下调对照组分别转染pc DNA3.1-H19、pc DNA3.1-control、si-H19、si-NC、miR-766-3p mimics、miR mimics-NC、miR-766-3p inhibitor、miR inhibitor-NC。除正常组外均缺氧24 h、复氧6 h。实时荧光定量聚合酶链反应(RT-q PCR)测定各组Lnc RNA H19、miR-766-3p表达;原位末端标记法(TUNEL)检测各组细胞凋亡率;RT-q PCR检测各组人c AMP激活的鸟苷酸交换蛋白(Epac-1)、B淋巴细胞瘤-2(Bcl-2)、胱天蛋白酶3(Capase3)基因表达;蛋白质免疫印迹法(Western Blot)检测各组上述蛋白表达;双荧光素酶报告基因实验探讨Lnc RNA H19与miR-766-3p的关系。结果:与正常组比较,空白组Lnc RNA H19、Epac-1表达均下降(P<0.05),miR-766-3p、Bcl-2、Capase3表达与细胞凋亡率均升高(P<0.05);与空白组比较,Lnc RNA H19上调组Lnc RNA H19、Epac-1表达均升高(P<0.05),miR-766-3p、Bcl-2、Capase3表达与细胞凋亡率均下降(P<0.05);Lnc RNA H19下调组Lnc RNA H19、Epac-1表达均下降(P<0.05),miR-766-3p、Bcl-2、Capase3表达与细胞凋亡率均升高(P<0.05);miR-766-3p上调组Epac-1表达下降(P<0.05),miR-766-3p、Bcl-2、Capase3表达与细胞凋亡率均升高(P<0.05);miR-766-3p下调组Epac-1表达升高(P<0.05),miR-766-3p、Bcl-2、Capase3表达与细胞凋亡率均下降(P<0.05)。Lnc RNA H19组miR-766-3p WT相对荧光素酶活性低于转染对照组(P<0.05),且Lnc RNA H19与miR-766-3p存在结合位点。结论:缺氧复氧导致大鼠心肌细胞株H9c2 LncRNA H19、Epac-1表达下降,而miR-766-3p、Bcl-2、Capase3表达与细胞凋亡率升高,但上调Lnc RNA H19可靶向抑制miR-766-3p减少细胞凋亡。 展开更多
关键词 缺氧复氧 心肌细胞 凋亡 长链非编码核糖核酸H19 微小核糖核酸-766-3p 实验研究
暂未订购
上一页 1 2 59 下一页 到第
使用帮助 返回顶部