Background Hybrid cotton enjoys overwhelming patronage among cotton farmers because of its superior yield capacity and fiber quality.However,various environmental factors affect its yield and fiber quality.This study ...Background Hybrid cotton enjoys overwhelming patronage among cotton farmers because of its superior yield capacity and fiber quality.However,various environmental factors affect its yield and fiber quality.This study aimed to assess 30 cotton hybrids for the stability of four traits(single-plant seed cotton yield,fiber upper half mean length(UHML),fiber strength,and micronaire)across three environments.Recent techniques,including genotype and genotype×environment(GGE)biplot,which provides a visual representation of performance and adaptability;weighted average absolute scores of the best linear unbiased predictions(WAASB),which balances the performance of the trait with stability;and multi-trait stability index(MTSI),which integrates multi-trait performance and stability,were used to analyze the stability of the four traits.Results Analysis of variance revealed significant genotype and environment interactions for all the traits studied,highlighting the need for comprehensive stability analysis.The environment E2 was the most suitable for the evaluation of seed cotton yield,whereas E3 was suitable for the evaluation of UHML and fiber strength.A stable hybrid,H05(TVH002×MCU5),with superior performance for seed cotton yield and UHML,was identified based on the overall results from GGE and WAASB.The which-won-where bioplot showed that H25(SVPR3×MCU5)performed the best for seed cotton yield in E3,and H27(Suraj×Sunantha)in E2 and E1.The hybrid H04(TVH002×CO14)in E1 and H30(Suraj×MCU5)in E2 and E3 performed well for UHML.Similarly,H28(Suraj×Suraksha)for E2 and E3 and H26(Suraj×Subiksha)for E1 were the best performing in the case of fiber strength.Based on the MTSI,four promising hybrids,namely,H24(SVPR3×CO14),H09(TVH2010×CO14),H18(MCU7×Suraksha),and H29(Suraj×CO14),were identified as stable with average performance for all four traits.Conclusions The study identified a stable hybrid,H05(TVH002×MCU5),with superior performance for yield and UHML.The identified hybrids in this study hold significant potential for cultivation across Tamil Nadu,with a scope for further evaluation in diverse environments.展开更多
With globalization there is an increasing need for spoken English for Chinese college students. However, the situation of the teaching system of spoken English in most universities in China is not satisfactory. Influe...With globalization there is an increasing need for spoken English for Chinese college students. However, the situation of the teaching system of spoken English in most universities in China is not satisfactory. Influenced by the exam-oriented teaching system, great importance has been put on English grammar and the skills of reading and writing. As a result, students'enthusiasm in improving their spoken English is diminished. Actually, students need some knowledge about English culture background before they can communicate effectively. It is significant for teachers to find out the students'wants and lacks during their spoken English study. Besides, attentions should be paid to English culture background in spoken English classes by both teachers and students. This paper mainly aimed at finding out the needs of students for English culture background in spoken English classes based on the theory of needs analysis.展开更多
Tolerance analysis of a planetary reducer is conducted. By focusing the serially-arranged parts in the central line and setting an appropriate dimension as a “gap”, worst-case tolerance analysis method and statistic...Tolerance analysis of a planetary reducer is conducted. By focusing the serially-arranged parts in the central line and setting an appropriate dimension as a “gap”, worst-case tolerance analysis method and statistical （RSS） tolerance analysis are used, and then a tolerance-analyzing tool CE/TOL is introduced. The acceptable value range of the “gap” is from 1.5 mm to 3.5 mm. While worst-case analysis rejects the design, RSS accepts it by a more realistic result with the law of probability accounted. With the help of CE/TOL and CAD software Pro/E, all components＇ dimensions and tolerances are assigned and modified, and more detailed and more comprehensible results are gives out.展开更多
The threshold of cyclin E expression at G1/S boundary is a characteristic feature of cell cycle progressing. In this study, we tried to develop a quantitative approach to analyze cyclin E threshold by multiparameter f...The threshold of cyclin E expression at G1/S boundary is a characteristic feature of cell cycle progressing. In this study, we tried to develop a quantitative approach to analyze cyclin E threshold by multiparameter flow cytometry. The expression of cyclin E in exponentially growing MOLT-4 cells was detected under different photomultiplier tube (PMT) voltages by cyclin E/DNA multiparameter flow cytometry. Additionally, cyclin E was detected in cells which were treated with caffeine and cycloheximide (CHX) under the same PMT voltage. Moreover, the expression of cyclin E in MOLT-4 cells was compared with that in JURKAT cells. Cyclin E threshold was quantified by formula B2/A×C (A, B, C indicates the minimum, threshold, and maximum of cyclin E fluorescence intensity, respectively). Results showed that in MOLT-4 cells, cyclin E threshold calculated by formula B2/A×C was invariable under different PMT settings. It was decreased in cells treated with caffeine and remained changeless in cells treated with cycloheximide. Cyclin E threshold in JURKAT cells was much lower than that in MOLT-4 cells. It was suggested that Formula B2/A×C we firstly set up could be used to analyze cyclin E expression threshold quantitatively.展开更多
OBJECTIVE： To investigate the association of E-selectin $128R polymorphisms with ischemic stroke. DATA SOURCES： A computer-based online search was conducted in PubMed, Elsevier, Ovid Database, the China National Kno...OBJECTIVE： To investigate the association of E-selectin $128R polymorphisms with ischemic stroke. DATA SOURCES： A computer-based online search was conducted in PubMed, Elsevier, Ovid Database, the China National Knowledge Infrastructure, and Wanfang Database between January 1998 and December 2010. STUDY SELECTION： Case-controlled studies addressing the association of the E-selectin polymorphism and ischemic stroke were included in this review. The genotype distribution complied with the Hardy-Weinberg genetic equilibrium. The included reports were evaluated by two authors for strict quality screening. Meta-analysis software, REVMAN 5.1, was used to investigate heterogeneity, pooled odds ratio （OR） and 95% confidence interval （CO in individual studies. MAIN OUTCOME MEASURES： Genotype and allele distributions at the E-selectin $128R site. RESULTS： Six case-controlled studies were included after screening and application of inclusion and exclusion criteria. There was no heterogeneity in the genotype and allele frequencies, and no publication bias was found. Meta-analysis of the pooled data showed that the OR value of the （AC＋CC）/AA genotype was 1.93 （95% CI： 1.55 2.41, Z= 5.80, P 〈 0.000 01）, and the ORfor the C/A allele was 1.80 （95% CI： 1.47 2.22, Z= 5.59, P 〈 0.000 01） in the ischemic stroke group, compared with control group. Results of pooled data in Chinese subjects showed that the ORvalue of （AC＋CC）/AA was 2.36 （95% CI： 1.68 3.31, Z = 4.99, P 〈 0.000 01）, and the OR value of the C/A allele was 2.25 （95% CI： 1.63 3.12, Z= 4.89, P 〈 0.000 01）. CONCLUSION： Polymorphism of E-selectin S128R was significantly associated with susceptibility to ischemic stroke; the AC and CC genotypes as well as the C allele may be factors associated with susceptibility to ischemic stroke.展开更多
BACKGROUND Cervical intraepithelial neoplasia(CIN)is an important precursor of cervical cancer.Early detection and treatment can reduce the incidence of cervical cancer.AIM To investigate the detection rate of human p...BACKGROUND Cervical intraepithelial neoplasia(CIN)is an important precursor of cervical cancer.Early detection and treatment can reduce the incidence of cervical cancer.AIM To investigate the detection rate of human papillomavirus(HPV)E6/E7 mRNA in cervical tissue of patients with different types of epithelial cell neoplasia(CIN)and its relationship with CIN progression and diagnosis.METHODS One hundred women with HPV infection detected by cervical exfoliation cytology between January 2022 and January 2023 were retrospectively selected.These patients were graded CIN based on colposcopy and cervical pathology.The positive expression rates of HPV E6/E7 mRNA and HPV[polymerase chain reaction(PCR)-reverse dot crossing]were compared among all groups.Patients with HPV E6/E7 mRNA expression in the grade 1 CIN group were followed up for 1 yr.The relationship between atypical squamous epithelium and high malignant epithelial neoplasia was investigated by univariate and multivariate analysis.RESULTS The diagnostic sensitivity,specificity,and sensitivity of PCR-reverse point hybrid ization technology for secondary CIN were 70.41%,70.66%,and 0.714,respectively.Sensitivity and specificity for secondary CIN were 752%and 7853%,respectively,the area under the curve value was 0.789.Logistic Multifactorial model analysis revealed that the HPV positive rates and the HPV E6/E7 mRNA positive rates were independent risk factors of CIN grade I(P<0.05).In CIN grade I patients with positive for HPV E6/E7 mRNA,in its orientation to grade CIN patients,in its orientation to grade CIN patients,at 69.2%,compared with patients negative for HPV E6/E7 mRNA(30.8%),significant difference(P<0.05).CONCLUSION HPV E6/E7 mRNA and HPV(PCR-reverse dot hybrid)positive expression have a close relationship with CINgrade disease progression and is an independent risk factor for high-grade CIN lesions.展开更多
Background： RING H2 finger E3 ligase （RH2FE3） genes encode cysteine rich proteins that mediate E3 ubiquitin ligase activity and degrade target substrates. The roles of these genes in plant responses to phytohormone...Background： RING H2 finger E3 ligase （RH2FE3） genes encode cysteine rich proteins that mediate E3 ubiquitin ligase activity and degrade target substrates. The roles of these genes in plant responses to phytohormones and abiotic stresses are well documented in various species, but their roles in cotton fiber development are poorly understood. To date, genome wide identification and expression analyses of Gossypium hirsutum RH2FE3 genes have not been reported. Methods： We performed computational identification, structural and phylogenetic analyses, chromosomal distribution analysis and estimated KJKs values of G hirsutum RH2FE3 genes. Orthologous and paralogous gene pairs were identified by all versus all BLASTP searches. We predicted cis regulatory elements and analyzed microarray data sets to generate heatmaps at different development stages. Tissue specific expression in cotton fiber, and hormonal and abiotic stress responses were determined by quantitative real time polymerase chain reaction （qRT PCR） analysis. Results： We investigated 140 G hirsutum, 80 G. orboreum, and evolutionary mechanisms and compared them with orthologs 89 G. roimondii putative RH2FB genes and their in Arobidopsis and rice. A domain based analysis of the G hirsutum RH2FE3 genes predicted conserved signature motifs and gene structures. Chromosomal localization showed the genes were distributed across all G hirsutum chromosomes, and 60 duplication events （4 tandem and 56 segmental duplications） and 98 orthologs were detected, cis elements were detected in the promoter regions of G hirsutum RH2FE3 genes. Microarray data and qRT PCR analyses showed that G hirsutum RH2FE3 genes were strongly correlated with cotton fiber development. Additionally, almost all the （brassinolide, gibberellic acid （GA）, indole 3-acetic acid drought, and salt）. dentified genes were up regulated in response to phytohormones （IAA）, and salicylic acid （SA）） and abiotic stresses （cold, heat, Conclusions： The genome wide identification, comprehensive analysis, and characterization of conserved domains and gene structures, as well as phylogenetic analysis, cis element prediction, and expression profile analysis of G hirsutum RH2FE3 genes and their roles in cotton fiber development and responses to plant hormones and abiotic stresses are reported here for the first time. Our findings will contribute to the genome wide analysis of putative RH2FE3 genes in other species and lay a foundation for future physiological and functional research on G hirsutum RH2FE3 genes.展开更多
This paper presents a generic procedure to implement a scalable and high performance data analysis framework for large-scale scientific simulation within an in-situ infrastructure. It demonstrates a unique capability ...This paper presents a generic procedure to implement a scalable and high performance data analysis framework for large-scale scientific simulation within an in-situ infrastructure. It demonstrates a unique capability for global Earth system simulations using advanced computing technologies (i.e., automated code analysis and instrumentation), in-situ infrastructure (i.e., ADIOS) and big data analysis engines (i.e., SciKit-learn). This paper also includes a useful case that analyzes a globe Earth System simulations with the integration of scalable in-situ infrastructure and advanced data processing package. The in-situ data analysis framework can provides new insights on scientific discoveries in multiscale modeling paradigms.展开更多
Objective To identify the important risk factors for type 2 Diabetes Mellitus(T2 DM) and develop effective strategies to address the problem of T2 DM. Our study aimed to evaluate the association between apolipoprotein...Objective To identify the important risk factors for type 2 Diabetes Mellitus(T2 DM) and develop effective strategies to address the problem of T2 DM. Our study aimed to evaluate the association between apolipoprotein E(Apo E) genetic polymorphism and type 2 diabetes, and to provide clues for the etiology of T2 DM.Methods Based on the criteria of inclusion and exclusion, we extracted, pooled, analyzed and assessed the case-control studies of Apo E polymorphism and T2 DM published in Pub Med, Web of Science,Medline, Wan Fang, VIP, and CNKI databases by R soft-ware(version 3.4.3). We used Random-effect models when heterogeneity was present in between-study, and fixed-effect models otherwise.Results We had 59 studies covering 6,872 cases with T2 DM and 8,250 controls, and compared the alleles and genotypes of Apo E between cases and controls. When we conducted a comparison between Apo E ε4 and ε3 alleles, we produced a pooled OR of 1.18(95% CI: 1.09-1.28;P < 0.001). Apo E ε2/ε2 genotype displayed a possible association with T2 DM(OR = 1.46;95% CI: 1.11-1.93;P = 0.007), ε3/ε4 genotype showed a 1.11-fold risk(OR = 1.11;95% CI: 1.01-1.22;P = 0.039) and ε4/ε4 genotype had a1.71-fold risk of developing T2 DM(OR = 1.71;95% CI: 1.33-2.19;P < 0.001) when they were compared with ε3/ε3 genotype.Conclusions There is an association between Apo E polymorphism and T2 DM: allele ε4 and genotypes(ε2/ε2, ε3/ε4, and ε4/ε4) are associated with the increased risk for the development of T2 DM, and they may be risk factors for T2 DM.展开更多
Abstract： Change detection is a standard tool to extract and analyze the earth＇s surface features from remotely sensed data. Among the different change detection techniques, change vector analysis （CVA） have an ex...Abstract： Change detection is a standard tool to extract and analyze the earth＇s surface features from remotely sensed data. Among the different change detection techniques, change vector analysis （CVA） have an exceptional advantage of discriminating change in terms of change magnitude and vector direction from multispectral bands. The estimation of precise threshold is one of the most crucial task in CVA to separate the change pixels from unchanged pixels because overall assessment of change detection method is highly dependent on selected threshold value. In recent years, integration of fuzzy clustering and remotely sensed data have become appropriate and realistic choice for change detection applications. The novelty of the proposed model lies within use of fuzzy maximum likelihood classification （FMLC） as fuzzy clustering in CVA. The FMLC based CVA is implemented using diverse threshold determination algorithms such as double-window flexible pace search （DFPS）, interactive trial and error （T＆E）, and 3x3-pixel kernel window （PKW）. Unlike existing CVA techniques, addition of fuzzy clustering in CVA permits each pixel to have multiple class categories and offers ease in threshold determination process. In present work, the comparative analysis has highlighted the performance of FMLC based CVA overimproved SCVA both in terms of accuracy assessment and operational complexity. Among all the examined threshold searching algorithms, FMLC based CVA using DFPS algorithm is found to be the most efficient method.展开更多
That flow is the common feature of substance flow and fluid flow is the viewpoint emphasized in the paper. Some notes on fluid mechanics, including the two approaches of fluid flow description, were given. The concept...That flow is the common feature of substance flow and fluid flow is the viewpoint emphasized in the paper. Some notes on fluid mechanics, including the two approaches of fluid flow description, were given. The concepts of the chain and the chain group of product life cycles, which are essential for understanding the specific features of substance flow, were advanced. Taking the specific feature of substance flow into consideration, on the analogy of the two approaches in fluid mechanics, two approaches of substance flow analysis, i.e. L method and E model, were formulated. Illustrative models of steady and unsteady substance flow were sketched by both methods, and comparison between them was made in general.展开更多
The e-mail network is a type of social network. This study analyzes user behavior in e-mail subject participation in organizations by using social network analysis. First, the Enron dataset and the position-related in...The e-mail network is a type of social network. This study analyzes user behavior in e-mail subject participation in organizations by using social network analysis. First, the Enron dataset and the position-related information of an employee are introduced, and methods for deletion of false data are presented. Next, the three-layer model(User, Subject, Keyword) is proposed for analysis of user behavior. Then, the proposed keyword selection algorithm based on a greedy approach, and the influence and propagation of an e-mail subject are defined. Finally, the e-mail user behavior is analyzed for the Enron organization. This study has considerable significance in subject recommendation and character recognition.展开更多
Supply Chain Management(SCM) is the term used to de sc ribe the management of the flow of materials, information and funds across the e ntire supply chain, from suppliers to component producers to final assemblers to ...Supply Chain Management(SCM) is the term used to de sc ribe the management of the flow of materials, information and funds across the e ntire supply chain, from suppliers to component producers to final assemblers to distribution(warehouses and retailers) and ultimately to the consumers.This pap er studies the coordinated problem for a manufacturing and retailing supply chai n. Differentiating from traditional joint optimization in the literature, the ap proach applies game theory structure as the researching framework to address the problem of coordinating the pricing decisions of the manufacturer and retailer. Three co-op pricing models are developed and discussed which are based on two noncooperative games and one cooperative game. They are a Stakelberg’s two-sta ge game model, a simultaneously move game model and an cooperative game model, r espectively. The results obtained by the sufficient comparisons and discusses te ll us that the noncooperative equilibriums are lower efficiently than joint coop erative equilibrium. In order to get the efficient cooperative mechanism, we int roduce the Pareto efficiency concept and obtain so-called feasible Pareto effic ient set of pricing schemes (contract curve). The cooperative mechanism based on the curve would be solid and stable. These and orther related managerial issues are explored in this paper.展开更多
Summary： A persistent infection model was established after human hepatoma cells infected by Japanese encephalitis viruses were subcultured for several times. Viral titers of mutant viruses in persistently infected c...Summary： A persistent infection model was established after human hepatoma cells infected by Japanese encephalitis viruses were subcultured for several times. Viral titers of mutant viruses in persistently infected cells were examined by plaque methods using BHK cells. Nucleotides of the E coding region of two wild and two mutant viruses were amplified by RT-PCR. PCR products were sequenced by ABI-PRSM^TM310 sequencing system. Compared to JaGAr-01 wild strains, four amino acids were replaced （E61Tyr→Asp, E219His→Tyr, E384Val→Glu, E418Pro→Ala） in the E sequence of JaGAr-01 persistently-infected mutant strains. Eleven amino acid replacement （E51Arg →Ser, E61Tyr→Asp, E83Lys→Glu, E123Ser→Arg, E209Arg→Lys, E227Pro→Ser, E276Asp→er, E290Arg→Lys, E387Lys→Arg, E418Leu→Pro, E454Arg→Gly） was also noted when we compared the E sequence between persistently infected Nakayama and its wild strains. A lot of similarities of amino acid sequence between mutant strains JaGAr-01 and Nakayama were also noted. It was concluded that geno-variation existed in E region of mutant viruses and the mutant protein encoded by E region, especially the mutation of E61 （Tyr→Asp） may contribute to the maintenance of the persistent infection of Japanese encephalitis virus.展开更多
OBJECTIVE： To evaluate the relationship between apolipoprotein E （ApoE） gene polymorphism and susceptibility to intracerebral hemorrhage （ICH） in Chinese population by a meta-analysis. METHODS： Related literatur...OBJECTIVE： To evaluate the relationship between apolipoprotein E （ApoE） gene polymorphism and susceptibility to intracerebral hemorrhage （ICH） in Chinese population by a meta-analysis. METHODS： Related literature regarding control analysis between ICH and control groups was collected. Independent case-control studies published between 1989 and 2007 that had complete data were included; and articles not closely related to the topic were excluded. The meta-analysis software, RevMan 4.2, was applied to analyze the odds ratio （OR） value in those studies included in the analysis to assess the relationship between susceptibility to ICH and ApoE polymorphism. RESULTS： Eight papers which were in accordance with the inclusion criteria were selected, and a total of 1 249 ICH cases and 1 329 controls were involved. Meta-analysis results showed that with the wildtype E3/3 as a reference, the OR values （95% confidence interval） of intracerebral hemorrhage for subjects carrying E2/2, E3/2, E4/2, E4/3, and E4/4 were 1.15 （0.60–2.21）, 1.00 （0.79–1.28）, 3.01 （1.73–5.23）, 1.78 （1.41–2.24） and 1.94 （1.03–3.65）, respectively. The combined OR values （95% confidence interval） of intracerebral hemorrhage for ε4 and ε2 carriers were 1.53 （1.16–2.01）, and 0.93 （0.69–1.25）. CONCLUSION： The results suggest that ApoE polymorphism is significantly associated with susceptibility to intracerebral hemorrhage and that ε4 carriers have a higher risk for intracerebral hemorrhage than others.展开更多
It is crucial to determine the spin and parity （jR） of Zc（3900）＋ for understanding its structure. We perform a helicity amplitude analysis on Zc（3900）＋ in the process e＋e- →π＋π-J/φ＊ with the hypotheses ...It is crucial to determine the spin and parity （jR） of Zc（3900）＋ for understanding its structure. We perform a helicity amplitude analysis on Zc（3900）＋ in the process e＋e- →π＋π-J/φ＊ with the hypotheses JP = 0-, 1＋, 1-, 2＋ and 2-. To estimate the significance of JP = 1＋ over other hypotheses, we perform a Monte Carlo simulation study, and we also discuss the prospect of measuring the spin parity in the future experiment with a large data sample.展开更多
[ Objective] This experiment aimed to find out the origin and genetic evolution relationship of chicken flavivirus （CFV） CJD05 strain in Fujian Province. [Method] A pair of primers were designed and synthesized acco...[ Objective] This experiment aimed to find out the origin and genetic evolution relationship of chicken flavivirus （CFV） CJD05 strain in Fujian Province. [Method] A pair of primers were designed and synthesized according to the sequences of E gene from Duck flavivirus （DFV） iso- late BYD-1. E gene of CFV isolate CJD05 was specially amplified and its sequences were analyzed. [Result] The target bar which was cloned from CFV isolate C, JD05 was I 503 bp. Homology analysis was conducted to compare E gene nucleotide sequence of CFV isolate CJD05 with DFV iso- late BYD-I and goose flavivirus （GFV） isolate JS804. Results indicated that isolate nuclectide homologies were 99.2% and 99.3%, and amino acid homologies were 99.0% and 98.6% respectively. [Conclusion] CFV isolate C, JD05, DFV isolate BYD-1 and GFV isolate JS804 were highly homologous. The homology of CFV isolate CJD05 with Tembusu virus （TMUV） was higher than with other arthropod-borne flaviviruses.展开更多
Minipreparation (MiniPrep) analysis is an essential step for obtaining a recombinant plasmid that carries a DNA insert containing a gene of interest. The most commonly used method for this involves cultivation of tran...Minipreparation (MiniPrep) analysis is an essential step for obtaining a recombinant plasmid that carries a DNA insert containing a gene of interest. The most commonly used method for this involves cultivation of transformed Escherichia coli (E. coli) in liquid medium, brief centrifugation for precipitation of bacterial pellets, and subsequent lysis of the pellets. This process is time-consuming and laborious, especially when the sample number is high. Here, we describe a more convenient method for MiniPrep analysis that utilizes solid medium-based cultivation of bacteria.展开更多
基金The TNAU Master’s student fellowship to the senior author。
文摘Background Hybrid cotton enjoys overwhelming patronage among cotton farmers because of its superior yield capacity and fiber quality.However,various environmental factors affect its yield and fiber quality.This study aimed to assess 30 cotton hybrids for the stability of four traits(single-plant seed cotton yield,fiber upper half mean length(UHML),fiber strength,and micronaire)across three environments.Recent techniques,including genotype and genotype×environment(GGE)biplot,which provides a visual representation of performance and adaptability;weighted average absolute scores of the best linear unbiased predictions(WAASB),which balances the performance of the trait with stability;and multi-trait stability index(MTSI),which integrates multi-trait performance and stability,were used to analyze the stability of the four traits.Results Analysis of variance revealed significant genotype and environment interactions for all the traits studied,highlighting the need for comprehensive stability analysis.The environment E2 was the most suitable for the evaluation of seed cotton yield,whereas E3 was suitable for the evaluation of UHML and fiber strength.A stable hybrid,H05(TVH002×MCU5),with superior performance for seed cotton yield and UHML,was identified based on the overall results from GGE and WAASB.The which-won-where bioplot showed that H25(SVPR3×MCU5)performed the best for seed cotton yield in E3,and H27(Suraj×Sunantha)in E2 and E1.The hybrid H04(TVH002×CO14)in E1 and H30(Suraj×MCU5)in E2 and E3 performed well for UHML.Similarly,H28(Suraj×Suraksha)for E2 and E3 and H26(Suraj×Subiksha)for E1 were the best performing in the case of fiber strength.Based on the MTSI,four promising hybrids,namely,H24(SVPR3×CO14),H09(TVH2010×CO14),H18(MCU7×Suraksha),and H29(Suraj×CO14),were identified as stable with average performance for all four traits.Conclusions The study identified a stable hybrid,H05(TVH002×MCU5),with superior performance for yield and UHML.The identified hybrids in this study hold significant potential for cultivation across Tamil Nadu,with a scope for further evaluation in diverse environments.
文摘With globalization there is an increasing need for spoken English for Chinese college students. However, the situation of the teaching system of spoken English in most universities in China is not satisfactory. Influenced by the exam-oriented teaching system, great importance has been put on English grammar and the skills of reading and writing. As a result, students'enthusiasm in improving their spoken English is diminished. Actually, students need some knowledge about English culture background before they can communicate effectively. It is significant for teachers to find out the students'wants and lacks during their spoken English study. Besides, attentions should be paid to English culture background in spoken English classes by both teachers and students. This paper mainly aimed at finding out the needs of students for English culture background in spoken English classes based on the theory of needs analysis.
文摘Tolerance analysis of a planetary reducer is conducted. By focusing the serially-arranged parts in the central line and setting an appropriate dimension as a “gap”, worst-case tolerance analysis method and statistical （RSS） tolerance analysis are used, and then a tolerance-analyzing tool CE/TOL is introduced. The acceptable value range of the “gap” is from 1.5 mm to 3.5 mm. While worst-case analysis rejects the design, RSS accepts it by a more realistic result with the law of probability accounted. With the help of CE/TOL and CAD software Pro/E, all components＇ dimensions and tolerances are assigned and modified, and more detailed and more comprehensible results are gives out.
基金This project was supported by grants from the NationalNatural Sciences Foundation of China ( No . 39672065 ,39730270 ,and 39725027) ,the China State Key Basic Re-search Program ( No . G1998051212) , and the ScienceFoundation of Ministry of Health, P. R. China ( No .20012537) .
文摘The threshold of cyclin E expression at G1/S boundary is a characteristic feature of cell cycle progressing. In this study, we tried to develop a quantitative approach to analyze cyclin E threshold by multiparameter flow cytometry. The expression of cyclin E in exponentially growing MOLT-4 cells was detected under different photomultiplier tube (PMT) voltages by cyclin E/DNA multiparameter flow cytometry. Additionally, cyclin E was detected in cells which were treated with caffeine and cycloheximide (CHX) under the same PMT voltage. Moreover, the expression of cyclin E in MOLT-4 cells was compared with that in JURKAT cells. Cyclin E threshold was quantified by formula B2/A×C (A, B, C indicates the minimum, threshold, and maximum of cyclin E fluorescence intensity, respectively). Results showed that in MOLT-4 cells, cyclin E threshold calculated by formula B2/A×C was invariable under different PMT settings. It was decreased in cells treated with caffeine and remained changeless in cells treated with cycloheximide. Cyclin E threshold in JURKAT cells was much lower than that in MOLT-4 cells. It was suggested that Formula B2/A×C we firstly set up could be used to analyze cyclin E expression threshold quantitatively.
文摘OBJECTIVE： To investigate the association of E-selectin $128R polymorphisms with ischemic stroke. DATA SOURCES： A computer-based online search was conducted in PubMed, Elsevier, Ovid Database, the China National Knowledge Infrastructure, and Wanfang Database between January 1998 and December 2010. STUDY SELECTION： Case-controlled studies addressing the association of the E-selectin polymorphism and ischemic stroke were included in this review. The genotype distribution complied with the Hardy-Weinberg genetic equilibrium. The included reports were evaluated by two authors for strict quality screening. Meta-analysis software, REVMAN 5.1, was used to investigate heterogeneity, pooled odds ratio （OR） and 95% confidence interval （CO in individual studies. MAIN OUTCOME MEASURES： Genotype and allele distributions at the E-selectin $128R site. RESULTS： Six case-controlled studies were included after screening and application of inclusion and exclusion criteria. There was no heterogeneity in the genotype and allele frequencies, and no publication bias was found. Meta-analysis of the pooled data showed that the OR value of the （AC＋CC）/AA genotype was 1.93 （95% CI： 1.55 2.41, Z= 5.80, P 〈 0.000 01）, and the ORfor the C/A allele was 1.80 （95% CI： 1.47 2.22, Z= 5.59, P 〈 0.000 01） in the ischemic stroke group, compared with control group. Results of pooled data in Chinese subjects showed that the ORvalue of （AC＋CC）/AA was 2.36 （95% CI： 1.68 3.31, Z = 4.99, P 〈 0.000 01）, and the OR value of the C/A allele was 2.25 （95% CI： 1.63 3.12, Z= 4.89, P 〈 0.000 01）. CONCLUSION： Polymorphism of E-selectin S128R was significantly associated with susceptibility to ischemic stroke; the AC and CC genotypes as well as the C allele may be factors associated with susceptibility to ischemic stroke.
基金Scientific Research Project of Hubei Provincial Health Commission,No.WJ2021M189。
文摘BACKGROUND Cervical intraepithelial neoplasia(CIN)is an important precursor of cervical cancer.Early detection and treatment can reduce the incidence of cervical cancer.AIM To investigate the detection rate of human papillomavirus(HPV)E6/E7 mRNA in cervical tissue of patients with different types of epithelial cell neoplasia(CIN)and its relationship with CIN progression and diagnosis.METHODS One hundred women with HPV infection detected by cervical exfoliation cytology between January 2022 and January 2023 were retrospectively selected.These patients were graded CIN based on colposcopy and cervical pathology.The positive expression rates of HPV E6/E7 mRNA and HPV[polymerase chain reaction(PCR)-reverse dot crossing]were compared among all groups.Patients with HPV E6/E7 mRNA expression in the grade 1 CIN group were followed up for 1 yr.The relationship between atypical squamous epithelium and high malignant epithelial neoplasia was investigated by univariate and multivariate analysis.RESULTS The diagnostic sensitivity,specificity,and sensitivity of PCR-reverse point hybrid ization technology for secondary CIN were 70.41%,70.66%,and 0.714,respectively.Sensitivity and specificity for secondary CIN were 752%and 7853%,respectively,the area under the curve value was 0.789.Logistic Multifactorial model analysis revealed that the HPV positive rates and the HPV E6/E7 mRNA positive rates were independent risk factors of CIN grade I(P<0.05).In CIN grade I patients with positive for HPV E6/E7 mRNA,in its orientation to grade CIN patients,in its orientation to grade CIN patients,at 69.2%,compared with patients negative for HPV E6/E7 mRNA(30.8%),significant difference(P<0.05).CONCLUSION HPV E6/E7 mRNA and HPV(PCR-reverse dot hybrid)positive expression have a close relationship with CINgrade disease progression and is an independent risk factor for high-grade CIN lesions.
基金supported by the Major Research Plan of National Natural Science Foundation of China(NO.31690093)Young Elite Scientist Sponsorship Program by CAST(China Association for Science and Technology)
文摘Background： RING H2 finger E3 ligase （RH2FE3） genes encode cysteine rich proteins that mediate E3 ubiquitin ligase activity and degrade target substrates. The roles of these genes in plant responses to phytohormones and abiotic stresses are well documented in various species, but their roles in cotton fiber development are poorly understood. To date, genome wide identification and expression analyses of Gossypium hirsutum RH2FE3 genes have not been reported. Methods： We performed computational identification, structural and phylogenetic analyses, chromosomal distribution analysis and estimated KJKs values of G hirsutum RH2FE3 genes. Orthologous and paralogous gene pairs were identified by all versus all BLASTP searches. We predicted cis regulatory elements and analyzed microarray data sets to generate heatmaps at different development stages. Tissue specific expression in cotton fiber, and hormonal and abiotic stress responses were determined by quantitative real time polymerase chain reaction （qRT PCR） analysis. Results： We investigated 140 G hirsutum, 80 G. orboreum, and evolutionary mechanisms and compared them with orthologs 89 G. roimondii putative RH2FB genes and their in Arobidopsis and rice. A domain based analysis of the G hirsutum RH2FE3 genes predicted conserved signature motifs and gene structures. Chromosomal localization showed the genes were distributed across all G hirsutum chromosomes, and 60 duplication events （4 tandem and 56 segmental duplications） and 98 orthologs were detected, cis elements were detected in the promoter regions of G hirsutum RH2FE3 genes. Microarray data and qRT PCR analyses showed that G hirsutum RH2FE3 genes were strongly correlated with cotton fiber development. Additionally, almost all the （brassinolide, gibberellic acid （GA）, indole 3-acetic acid drought, and salt）. dentified genes were up regulated in response to phytohormones （IAA）, and salicylic acid （SA）） and abiotic stresses （cold, heat, Conclusions： The genome wide identification, comprehensive analysis, and characterization of conserved domains and gene structures, as well as phylogenetic analysis, cis element prediction, and expression profile analysis of G hirsutum RH2FE3 genes and their roles in cotton fiber development and responses to plant hormones and abiotic stresses are reported here for the first time. Our findings will contribute to the genome wide analysis of putative RH2FE3 genes in other species and lay a foundation for future physiological and functional research on G hirsutum RH2FE3 genes.
文摘This paper presents a generic procedure to implement a scalable and high performance data analysis framework for large-scale scientific simulation within an in-situ infrastructure. It demonstrates a unique capability for global Earth system simulations using advanced computing technologies (i.e., automated code analysis and instrumentation), in-situ infrastructure (i.e., ADIOS) and big data analysis engines (i.e., SciKit-learn). This paper also includes a useful case that analyzes a globe Earth System simulations with the integration of scalable in-situ infrastructure and advanced data processing package. The in-situ data analysis framework can provides new insights on scientific discoveries in multiscale modeling paradigms.
基金supported by the Jipa Ruida Environmental Inspection Corporation Limited,Beijing under Grant Radioactive Diagnosis and Treatment Construction Project-Radiation Protection and Evaluation [Grant No.2016YX137]Jilin Province Pharmacy Operation Corporation,Limited [Grant No.371182093427]
文摘Objective To identify the important risk factors for type 2 Diabetes Mellitus(T2 DM) and develop effective strategies to address the problem of T2 DM. Our study aimed to evaluate the association between apolipoprotein E(Apo E) genetic polymorphism and type 2 diabetes, and to provide clues for the etiology of T2 DM.Methods Based on the criteria of inclusion and exclusion, we extracted, pooled, analyzed and assessed the case-control studies of Apo E polymorphism and T2 DM published in Pub Med, Web of Science,Medline, Wan Fang, VIP, and CNKI databases by R soft-ware(version 3.4.3). We used Random-effect models when heterogeneity was present in between-study, and fixed-effect models otherwise.Results We had 59 studies covering 6,872 cases with T2 DM and 8,250 controls, and compared the alleles and genotypes of Apo E between cases and controls. When we conducted a comparison between Apo E ε4 and ε3 alleles, we produced a pooled OR of 1.18(95% CI: 1.09-1.28;P < 0.001). Apo E ε2/ε2 genotype displayed a possible association with T2 DM(OR = 1.46;95% CI: 1.11-1.93;P = 0.007), ε3/ε4 genotype showed a 1.11-fold risk(OR = 1.11;95% CI: 1.01-1.22;P = 0.039) and ε4/ε4 genotype had a1.71-fold risk of developing T2 DM(OR = 1.71;95% CI: 1.33-2.19;P < 0.001) when they were compared with ε3/ε3 genotype.Conclusions There is an association between Apo E polymorphism and T2 DM: allele ε4 and genotypes(ε2/ε2, ε3/ε4, and ε4/ε4) are associated with the increased risk for the development of T2 DM, and they may be risk factors for T2 DM.
文摘Abstract： Change detection is a standard tool to extract and analyze the earth＇s surface features from remotely sensed data. Among the different change detection techniques, change vector analysis （CVA） have an exceptional advantage of discriminating change in terms of change magnitude and vector direction from multispectral bands. The estimation of precise threshold is one of the most crucial task in CVA to separate the change pixels from unchanged pixels because overall assessment of change detection method is highly dependent on selected threshold value. In recent years, integration of fuzzy clustering and remotely sensed data have become appropriate and realistic choice for change detection applications. The novelty of the proposed model lies within use of fuzzy maximum likelihood classification （FMLC） as fuzzy clustering in CVA. The FMLC based CVA is implemented using diverse threshold determination algorithms such as double-window flexible pace search （DFPS）, interactive trial and error （T＆E）, and 3x3-pixel kernel window （PKW）. Unlike existing CVA techniques, addition of fuzzy clustering in CVA permits each pixel to have multiple class categories and offers ease in threshold determination process. In present work, the comparative analysis has highlighted the performance of FMLC based CVA overimproved SCVA both in terms of accuracy assessment and operational complexity. Among all the examined threshold searching algorithms, FMLC based CVA using DFPS algorithm is found to be the most efficient method.
文摘That flow is the common feature of substance flow and fluid flow is the viewpoint emphasized in the paper. Some notes on fluid mechanics, including the two approaches of fluid flow description, were given. The concepts of the chain and the chain group of product life cycles, which are essential for understanding the specific features of substance flow, were advanced. Taking the specific feature of substance flow into consideration, on the analogy of the two approaches in fluid mechanics, two approaches of substance flow analysis, i.e. L method and E model, were formulated. Illustrative models of steady and unsteady substance flow were sketched by both methods, and comparison between them was made in general.
基金sponsored by the National Natural Science Foundation of China under grant number No.61100008,61201084the China Postdoctoral Science Foundation under Grant No.2013M541346+3 种基金Heilongiiang Postdoctoral Special Fund(Postdoctoral Youth Talent Program)under Grant No.LBH-TZ0504Heilongjiang Postdoctoral Fund under Grant No.LBH-Z13058the Natural Science Foundation of Heilongjiang Province of China under Grant No.QC2015076The Fundamental Research Funds for the Central Universities of China under grant number HEUCF100602
文摘The e-mail network is a type of social network. This study analyzes user behavior in e-mail subject participation in organizations by using social network analysis. First, the Enron dataset and the position-related information of an employee are introduced, and methods for deletion of false data are presented. Next, the three-layer model(User, Subject, Keyword) is proposed for analysis of user behavior. Then, the proposed keyword selection algorithm based on a greedy approach, and the influence and propagation of an e-mail subject are defined. Finally, the e-mail user behavior is analyzed for the Enron organization. This study has considerable significance in subject recommendation and character recognition.
文摘Supply Chain Management(SCM) is the term used to de sc ribe the management of the flow of materials, information and funds across the e ntire supply chain, from suppliers to component producers to final assemblers to distribution(warehouses and retailers) and ultimately to the consumers.This pap er studies the coordinated problem for a manufacturing and retailing supply chai n. Differentiating from traditional joint optimization in the literature, the ap proach applies game theory structure as the researching framework to address the problem of coordinating the pricing decisions of the manufacturer and retailer. Three co-op pricing models are developed and discussed which are based on two noncooperative games and one cooperative game. They are a Stakelberg’s two-sta ge game model, a simultaneously move game model and an cooperative game model, r espectively. The results obtained by the sufficient comparisons and discusses te ll us that the noncooperative equilibriums are lower efficiently than joint coop erative equilibrium. In order to get the efficient cooperative mechanism, we int roduce the Pareto efficiency concept and obtain so-called feasible Pareto effic ient set of pricing schemes (contract curve). The cooperative mechanism based on the curve would be solid and stable. These and orther related managerial issues are explored in this paper.
文摘Summary： A persistent infection model was established after human hepatoma cells infected by Japanese encephalitis viruses were subcultured for several times. Viral titers of mutant viruses in persistently infected cells were examined by plaque methods using BHK cells. Nucleotides of the E coding region of two wild and two mutant viruses were amplified by RT-PCR. PCR products were sequenced by ABI-PRSM^TM310 sequencing system. Compared to JaGAr-01 wild strains, four amino acids were replaced （E61Tyr→Asp, E219His→Tyr, E384Val→Glu, E418Pro→Ala） in the E sequence of JaGAr-01 persistently-infected mutant strains. Eleven amino acid replacement （E51Arg →Ser, E61Tyr→Asp, E83Lys→Glu, E123Ser→Arg, E209Arg→Lys, E227Pro→Ser, E276Asp→er, E290Arg→Lys, E387Lys→Arg, E418Leu→Pro, E454Arg→Gly） was also noted when we compared the E sequence between persistently infected Nakayama and its wild strains. A lot of similarities of amino acid sequence between mutant strains JaGAr-01 and Nakayama were also noted. It was concluded that geno-variation existed in E region of mutant viruses and the mutant protein encoded by E region, especially the mutation of E61 （Tyr→Asp） may contribute to the maintenance of the persistent infection of Japanese encephalitis virus.
文摘OBJECTIVE： To evaluate the relationship between apolipoprotein E （ApoE） gene polymorphism and susceptibility to intracerebral hemorrhage （ICH） in Chinese population by a meta-analysis. METHODS： Related literature regarding control analysis between ICH and control groups was collected. Independent case-control studies published between 1989 and 2007 that had complete data were included; and articles not closely related to the topic were excluded. The meta-analysis software, RevMan 4.2, was applied to analyze the odds ratio （OR） value in those studies included in the analysis to assess the relationship between susceptibility to ICH and ApoE polymorphism. RESULTS： Eight papers which were in accordance with the inclusion criteria were selected, and a total of 1 249 ICH cases and 1 329 controls were involved. Meta-analysis results showed that with the wildtype E3/3 as a reference, the OR values （95% confidence interval） of intracerebral hemorrhage for subjects carrying E2/2, E3/2, E4/2, E4/3, and E4/4 were 1.15 （0.60–2.21）, 1.00 （0.79–1.28）, 3.01 （1.73–5.23）, 1.78 （1.41–2.24） and 1.94 （1.03–3.65）, respectively. The combined OR values （95% confidence interval） of intracerebral hemorrhage for ε4 and ε2 carriers were 1.53 （1.16–2.01）, and 0.93 （0.69–1.25）. CONCLUSION： The results suggest that ApoE polymorphism is significantly associated with susceptibility to intracerebral hemorrhage and that ε4 carriers have a higher risk for intracerebral hemorrhage than others.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11175146 and 11375205the Netherlands Project CEP under Grant No 530-4CDP03
文摘It is crucial to determine the spin and parity （jR） of Zc（3900）＋ for understanding its structure. We perform a helicity amplitude analysis on Zc（3900）＋ in the process e＋e- →π＋π-J/φ＊ with the hypotheses JP = 0-, 1＋, 1-, 2＋ and 2-. To estimate the significance of JP = 1＋ over other hypotheses, we perform a Monte Carlo simulation study, and we also discuss the prospect of measuring the spin parity in the future experiment with a large data sample.
基金Innovation Team project(STIF-Y02),FuJian Academy of Agriculture SciencesFujian Scientific Research Institutes of Public Welfare Special Fund(2011R1025-2)
文摘[ Objective] This experiment aimed to find out the origin and genetic evolution relationship of chicken flavivirus （CFV） CJD05 strain in Fujian Province. [Method] A pair of primers were designed and synthesized according to the sequences of E gene from Duck flavivirus （DFV） iso- late BYD-1. E gene of CFV isolate CJD05 was specially amplified and its sequences were analyzed. [Result] The target bar which was cloned from CFV isolate C, JD05 was I 503 bp. Homology analysis was conducted to compare E gene nucleotide sequence of CFV isolate CJD05 with DFV iso- late BYD-I and goose flavivirus （GFV） isolate JS804. Results indicated that isolate nuclectide homologies were 99.2% and 99.3%, and amino acid homologies were 99.0% and 98.6% respectively. [Conclusion] CFV isolate C, JD05, DFV isolate BYD-1 and GFV isolate JS804 were highly homologous. The homology of CFV isolate CJD05 with Tembusu virus （TMUV） was higher than with other arthropod-borne flaviviruses.
文摘Minipreparation (MiniPrep) analysis is an essential step for obtaining a recombinant plasmid that carries a DNA insert containing a gene of interest. The most commonly used method for this involves cultivation of transformed Escherichia coli (E. coli) in liquid medium, brief centrifugation for precipitation of bacterial pellets, and subsequent lysis of the pellets. This process is time-consuming and laborious, especially when the sample number is high. Here, we describe a more convenient method for MiniPrep analysis that utilizes solid medium-based cultivation of bacteria.
