A down-scaled operational oceanographic system is developed for the coastal waters of Korea using a re- gional ocean modeling system （ROMS）. The operational oceanographic modeling system consists of at- mospheric an...A down-scaled operational oceanographic system is developed for the coastal waters of Korea using a re- gional ocean modeling system （ROMS）. The operational oceanographic modeling system consists of at- mospheric and hydrodynamic models. The hydrodynamic model, ROMS, is coupled with wave, sediment transport, and water quality modules. The system forecasts the predicted results twice a day on a 72 h basis, including sea surface elevation, currents, temperature, salinity, storm surge height, and wave information for the coastal waters of Korea. The predicted results are exported to the web-GIS-based coastal informa- tion system for real-time dissemination to the public and validation with real-time monitoring data using visualization technologies. The ROMS is two-way coupled with a simulating waves nearshore model, SWAN, for the hydrodynamics and waves, nested with the meteorological model, WRE for the atmospheric surface forcing, and externally nested with the eutrophication model, CE-QUAL-ICM, for the water quality. The op- erational model, ROMS, was calibrated with the tidal surface observed with a tide-gage and verified with current data observed by bottom-mounted ADCP or AWAC near the coastal waters of Korea. To validate the predicted results, we used real-time monitoring data derived from remote buoy system, HF-radar, and geostationary ocean color imager （GOCI）. This down-scaled operational coastal forecasting system will be used as a part of the Korea operational oceanographic system （KOOS） with other operational oceanographic systems.展开更多
Although the coding modes of H.264 coded video would be changed by the transcoding process of spatial resolution reduction, there exists good correlation in prediction modes and prediction directions between input and...Although the coding modes of H.264 coded video would be changed by the transcoding process of spatial resolution reduction, there exists good correlation in prediction modes and prediction directions between input and output video. In this paper, we first introduce a new spatial resolution reduction transcoding architecture of intra coded frames where the distortion can be calculated directly in compression domain. We then propose a fast mode decision algorithm in which only a small part of rate distortion optimization （RDO） calculation is needed for mode decision. For 4×4 luma block, the proposed scheme has average 21.3% computation saving, compared to the cascaded pixel-domain transcoding scheme with the fast intra mode decision algorithm proposed in JVT-G013. For 16×16 luma block, RDO calculation is completely avoided in our scheme while the scheme in JVT-G013 needs 2 RDO calculations. Experimental results show that our scheme outperforms that of JVT-G013 in terms of significantly computasavings with negligible loss of PSNR展开更多
目的通过构建15 L缩小模型验证重组人干扰素(recombinant human interferon,rhIFN)α2b工作代菌种在关键代次的传代稳定性,以确保rhIFNα2b在生产过程中的稳定性和有效性。方法将rhIFNα2b冻干菌种复苏并进行传代培养,分别对第0、10、20...目的通过构建15 L缩小模型验证重组人干扰素(recombinant human interferon,rhIFN)α2b工作代菌种在关键代次的传代稳定性,以确保rhIFNα2b在生产过程中的稳定性和有效性。方法将rhIFNα2b冻干菌种复苏并进行传代培养,分别对第0、10、20、30、40、50代菌液保存甘油菌。选取传代稳定性研究中保存的各代次甘油菌样品,在模拟生产条件的15 L缩小模型发酵系统中进行发酵-表达试验,通过实时监测菌体生长曲线,结合终点检测指标(产物表达量、生物学活性、质粒稳定性、遗传完整性以及微生物纯度等)进行全面比对,以判断限传代次是否可覆盖现有培养工艺。结果rhIFNα2b工作代菌种第0、10、20、30、40和50代甘油菌在连续传代培养后,表现出良好的稳定性和一致性。各代次菌种在LB平板上均呈现典型的大肠埃希菌菌落形态,无杂菌污染;抗生素抗性未发生改变;革兰染色结果均为革兰阴性;生化反应特性符合大肠埃希菌典型特征;目标基因表达量始终高于20%,且无下降趋势;发酵活性维持在1.0×10^(8)IU/L以上;质粒酶切图谱与理论图谱一致,序列未发生变化,质粒丢失率<30%,表现出较好的遗传稳定性。结论rhIFNα2b工作代菌种在缩小模型模拟生产规模发酵时发酵培养的稳定性良好,现有工艺条件下菌种的限传代次能够有效覆盖变更后的生产需求,为工艺优化提供了重要的数据支撑。展开更多
基金The project entitled Cooperation on the Development of Basic Technologies for the Yellow Sea and East China Sea Operational Oceanographic System funded by the China-Korea Joint Ocean Research Centerthe project entitled"Development of Korea Operational Oceanographic System"funded by the Ministry of Oceans and Fisheries,Koreathe project Functional Improvement of Korea Ocean Satellite Center and Development of the Marine Environment Impact Prediction Program funded by the Korea Institute of Ocean Science and Technology
文摘A down-scaled operational oceanographic system is developed for the coastal waters of Korea using a re- gional ocean modeling system （ROMS）. The operational oceanographic modeling system consists of at- mospheric and hydrodynamic models. The hydrodynamic model, ROMS, is coupled with wave, sediment transport, and water quality modules. The system forecasts the predicted results twice a day on a 72 h basis, including sea surface elevation, currents, temperature, salinity, storm surge height, and wave information for the coastal waters of Korea. The predicted results are exported to the web-GIS-based coastal informa- tion system for real-time dissemination to the public and validation with real-time monitoring data using visualization technologies. The ROMS is two-way coupled with a simulating waves nearshore model, SWAN, for the hydrodynamics and waves, nested with the meteorological model, WRE for the atmospheric surface forcing, and externally nested with the eutrophication model, CE-QUAL-ICM, for the water quality. The op- erational model, ROMS, was calibrated with the tidal surface observed with a tide-gage and verified with current data observed by bottom-mounted ADCP or AWAC near the coastal waters of Korea. To validate the predicted results, we used real-time monitoring data derived from remote buoy system, HF-radar, and geostationary ocean color imager （GOCI）. This down-scaled operational coastal forecasting system will be used as a part of the Korea operational oceanographic system （KOOS） with other operational oceanographic systems.
文摘Although the coding modes of H.264 coded video would be changed by the transcoding process of spatial resolution reduction, there exists good correlation in prediction modes and prediction directions between input and output video. In this paper, we first introduce a new spatial resolution reduction transcoding architecture of intra coded frames where the distortion can be calculated directly in compression domain. We then propose a fast mode decision algorithm in which only a small part of rate distortion optimization （RDO） calculation is needed for mode decision. For 4×4 luma block, the proposed scheme has average 21.3% computation saving, compared to the cascaded pixel-domain transcoding scheme with the fast intra mode decision algorithm proposed in JVT-G013. For 16×16 luma block, RDO calculation is completely avoided in our scheme while the scheme in JVT-G013 needs 2 RDO calculations. Experimental results show that our scheme outperforms that of JVT-G013 in terms of significantly computasavings with negligible loss of PSNR
文摘目的通过构建15 L缩小模型验证重组人干扰素(recombinant human interferon,rhIFN)α2b工作代菌种在关键代次的传代稳定性,以确保rhIFNα2b在生产过程中的稳定性和有效性。方法将rhIFNα2b冻干菌种复苏并进行传代培养,分别对第0、10、20、30、40、50代菌液保存甘油菌。选取传代稳定性研究中保存的各代次甘油菌样品,在模拟生产条件的15 L缩小模型发酵系统中进行发酵-表达试验,通过实时监测菌体生长曲线,结合终点检测指标(产物表达量、生物学活性、质粒稳定性、遗传完整性以及微生物纯度等)进行全面比对,以判断限传代次是否可覆盖现有培养工艺。结果rhIFNα2b工作代菌种第0、10、20、30、40和50代甘油菌在连续传代培养后,表现出良好的稳定性和一致性。各代次菌种在LB平板上均呈现典型的大肠埃希菌菌落形态,无杂菌污染;抗生素抗性未发生改变;革兰染色结果均为革兰阴性;生化反应特性符合大肠埃希菌典型特征;目标基因表达量始终高于20%,且无下降趋势;发酵活性维持在1.0×10^(8)IU/L以上;质粒酶切图谱与理论图谱一致,序列未发生变化,质粒丢失率<30%,表现出较好的遗传稳定性。结论rhIFNα2b工作代菌种在缩小模型模拟生产规模发酵时发酵培养的稳定性良好,现有工艺条件下菌种的限传代次能够有效覆盖变更后的生产需求,为工艺优化提供了重要的数据支撑。
