Digital microfluidic(DMF)technology is widely used in bioanalysis and chemical reactions due to its accuracy and flexibility in manipulating droplets.However,most DMF systems usually rely on complex electrode fabricat...Digital microfluidic(DMF)technology is widely used in bioanalysis and chemical reactions due to its accuracy and flexibility in manipulating droplets.However,most DMF systems usually rely on complex electrode fabrication and high driving voltages.Sensor integration in DMF systems is also quite rare.In this study,a programmable magnetic digital microfluidic(PMDMF)platform integrated with electrochemical detection system was proposed.It enables non-contact,flexible droplet manipulation without complex processes and high voltages,meeting the requirements of automated electrochemical detection.The platform includes a magnetic control system,a microfluidic chip,and an electrochemical detection system.The magnetic control system consists of a microcoil array circuit board,a N52 permanent magnet,and an Arduino control module.N52 magnets generate localized magnetic fields to drive droplet movement,while the Arduino module enables programmable control for precise manipulation.The maximum average velocity of the droplet is about 3.9 cm/s.The microfluidic chip was fabricated using 3D printing and the superhydrophobic surface of chip was fabricated by spray coating.The electrochemical detection system consists of the MoS_(2)@CeO_(2)/PVA working electrode,Ag/AgCl reference electrode,and carbon counter electrode.To evaluate the practical value of the integrated platform,glucose in sweat was automatically and accurately detected.The proposed platform has a wide linear detection range(0.01–0.25 mM),a lower LOD(6.5μM),a superior sensitivity(7833.54μA·mM^(−1)·cm^(−2)),and excellent recovery rate(88.1-113.5%).It has an extensive potential for future application in the fields of medical diagnostics and point-of-care testing.展开更多
Two improved structures of high resolution digital pulse width modulator(DPWM) control circuit are proposed. Embedded digital clock manager(DCM) blocks and digital programmable delay circuits are employed as the basic...Two improved structures of high resolution digital pulse width modulator(DPWM) control circuit are proposed. Embedded digital clock manager(DCM) blocks and digital programmable delay circuits are employed as the basic resources to construct the field-programmable gate array(FPGA)-based DPWM implementations. Detailed schemes are illustrated and the circuits have been successfully implemented on the Artix-7 FPGA device developed by Xilinx. Experimental results show that when the basic clock operates at the frequency of 200 MHz, the resolutions of the two approaches can reach 625 ps and 500 ps, respectively. Besides,the presented schemes possess other merits including flexible resolution, strong versatility and relatively good stability.展开更多
基金supported by grants from the National Key Research and Development Program of China(No.2023YFB3208200)the equipment research and development projects of the Chinese Academy of Sciences(PTYQ2024YZ0010)+3 种基金the Science and Technology Commission of Shanghai Municipality Project(XTCX-KJ-2024-038)National Natural Science Foundation of China(62401555)Shanghai Science and Technology Development Funds(23J21900100)supported by the Postdoctoral Fellowship Program of CPSF under Grant Number GZC20232838.
文摘Digital microfluidic(DMF)technology is widely used in bioanalysis and chemical reactions due to its accuracy and flexibility in manipulating droplets.However,most DMF systems usually rely on complex electrode fabrication and high driving voltages.Sensor integration in DMF systems is also quite rare.In this study,a programmable magnetic digital microfluidic(PMDMF)platform integrated with electrochemical detection system was proposed.It enables non-contact,flexible droplet manipulation without complex processes and high voltages,meeting the requirements of automated electrochemical detection.The platform includes a magnetic control system,a microfluidic chip,and an electrochemical detection system.The magnetic control system consists of a microcoil array circuit board,a N52 permanent magnet,and an Arduino control module.N52 magnets generate localized magnetic fields to drive droplet movement,while the Arduino module enables programmable control for precise manipulation.The maximum average velocity of the droplet is about 3.9 cm/s.The microfluidic chip was fabricated using 3D printing and the superhydrophobic surface of chip was fabricated by spray coating.The electrochemical detection system consists of the MoS_(2)@CeO_(2)/PVA working electrode,Ag/AgCl reference electrode,and carbon counter electrode.To evaluate the practical value of the integrated platform,glucose in sweat was automatically and accurately detected.The proposed platform has a wide linear detection range(0.01–0.25 mM),a lower LOD(6.5μM),a superior sensitivity(7833.54μA·mM^(−1)·cm^(−2)),and excellent recovery rate(88.1-113.5%).It has an extensive potential for future application in the fields of medical diagnostics and point-of-care testing.
基金supported by the National Natural Science Foundation of China(61401204)the Fundamental Research Funds for the Central Universities(30916011319)+1 种基金the Technology Research and Development Program of Jiangsu Province(BY2015004-03)the Postdoctoral Science Foundation of Jiangsu Province(1501104C)
文摘Two improved structures of high resolution digital pulse width modulator(DPWM) control circuit are proposed. Embedded digital clock manager(DCM) blocks and digital programmable delay circuits are employed as the basic resources to construct the field-programmable gate array(FPGA)-based DPWM implementations. Detailed schemes are illustrated and the circuits have been successfully implemented on the Artix-7 FPGA device developed by Xilinx. Experimental results show that when the basic clock operates at the frequency of 200 MHz, the resolutions of the two approaches can reach 625 ps and 500 ps, respectively. Besides,the presented schemes possess other merits including flexible resolution, strong versatility and relatively good stability.
