This study examined the isolation and differentiation of dorsal root ganglion progenitor cells for therapeutic use in neurodegenerative diseases. Rat embryonic dorsal root ganglia progenitors were isolated and purifie...This study examined the isolation and differentiation of dorsal root ganglion progenitor cells for therapeutic use in neurodegenerative diseases. Rat embryonic dorsal root ganglia progenitors were isolated and purified using the differential adhesion method combined with cytosine arabinoside treatment. After culture in serum-free medium supplemented with B27, basic fibroblast growth factor and epidermal growth factor, these cells remained viable and survived for more than 18 months in vitro. Most cells differentiated to neurons that were immunoreactive for gamma-aminobutyric acid and choline acetyltransferase as detected by immunohistochemical staining. In addition, nerve growth factor and neurotrophic tyrosine kinase receptor expression were also observed in dorsal root ganglion progenitors and differentiated cells. K252a, an inhibitor that blocks nerve growth factor-induced signaling, inhibited cell survival, suggesting the possible existence of a nerve growth factor autocrine loop in these proliferating cells.展开更多
The improved tetraploids(G1×AT)were obtained by distant crossing and gynogenesis and the high-body individuals accounted for 2%among G1×AT.After mating with each other,the high-body in-dividuals produced thr...The improved tetraploids(G1×AT)were obtained by distant crossing and gynogenesis and the high-body individuals accounted for 2%among G1×AT.After mating with each other,the high-body in-dividuals produced three kinds of bisexual fertile diploid fishes:high-body red crucian carp,high-body fork-like-tails goldfish and gray common carp.The high-body red crucian carp mating with each other formed three types of improved crucian carp(ICC)including improved red crucian carp(IRCC),im-proved color crucian carp(ICCC)and improved gray crucian carp(IGCC).The phenotypes,chromo-some numbers,gonadal structure and fertility of the three kinds of ICC and their offspring were observed.All the three kinds of ICC possessed some improved phenotypes such as higher body,smaller head and shorter tail.The ratios of the body height to body length of IRCC,ICCC and IGCC were 0.54,0.51 and 0.54,respectively.All of them were obviously higher than that of red crucian carp 0.41(P<0.01).Three kinds of ICC had the same chromosome number as red crucian carp with 100 chromosomes.All the ICC possessed normal gonads producing mature eggs or sperm,which was important for the production of an improved diploid population.Compared with red crucian carp,all the ICC had stronger fertility such as higher gametes production,higher fertilization rate and higher hatchery rate.Three types of improved diploid fish population were generated from the three kinds of ICC by self-crossing,respectively.The ICC can serve as ornamental fish and edible fish.They are also ideal parents to produce triploids by mating with tetraploids.The new ICC plays an important role in biological evolution and fish genetic breeding.展开更多
基金supported by the Rhone Alps Region of France, MIRA grants for the collaboration,the Agence National de la Recherche (ANR) RIB,the CNRS (International program for Scientific Cooperation, "PICS")
文摘This study examined the isolation and differentiation of dorsal root ganglion progenitor cells for therapeutic use in neurodegenerative diseases. Rat embryonic dorsal root ganglia progenitors were isolated and purified using the differential adhesion method combined with cytosine arabinoside treatment. After culture in serum-free medium supplemented with B27, basic fibroblast growth factor and epidermal growth factor, these cells remained viable and survived for more than 18 months in vitro. Most cells differentiated to neurons that were immunoreactive for gamma-aminobutyric acid and choline acetyltransferase as detected by immunohistochemical staining. In addition, nerve growth factor and neurotrophic tyrosine kinase receptor expression were also observed in dorsal root ganglion progenitors and differentiated cells. K252a, an inhibitor that blocks nerve growth factor-induced signaling, inhibited cell survival, suggesting the possible existence of a nerve growth factor autocrine loop in these proliferating cells.
基金the National Natural Science Fund for Distinguished Young Scholars(Grant No.30725028)National Natural Science Foundation of China(Grant Nos.30330480 and 30571444)+4 种基金National Basic Research Program(Grant No.2007CB-109206)Doctoral Station of University of the Education Ministry of China(Grant No.200405422001)Program for Changjiang Scholars and Innovative Research Team in University(Grant No.IRT0445)Agriculture Technological Production Foundation of the Science and Technology Ministry of China(Grant No.2005380)Important Solution Project of Hunan Province(Grant No.2006NK2008)
文摘The improved tetraploids(G1×AT)were obtained by distant crossing and gynogenesis and the high-body individuals accounted for 2%among G1×AT.After mating with each other,the high-body in-dividuals produced three kinds of bisexual fertile diploid fishes:high-body red crucian carp,high-body fork-like-tails goldfish and gray common carp.The high-body red crucian carp mating with each other formed three types of improved crucian carp(ICC)including improved red crucian carp(IRCC),im-proved color crucian carp(ICCC)and improved gray crucian carp(IGCC).The phenotypes,chromo-some numbers,gonadal structure and fertility of the three kinds of ICC and their offspring were observed.All the three kinds of ICC possessed some improved phenotypes such as higher body,smaller head and shorter tail.The ratios of the body height to body length of IRCC,ICCC and IGCC were 0.54,0.51 and 0.54,respectively.All of them were obviously higher than that of red crucian carp 0.41(P<0.01).Three kinds of ICC had the same chromosome number as red crucian carp with 100 chromosomes.All the ICC possessed normal gonads producing mature eggs or sperm,which was important for the production of an improved diploid population.Compared with red crucian carp,all the ICC had stronger fertility such as higher gametes production,higher fertilization rate and higher hatchery rate.Three types of improved diploid fish population were generated from the three kinds of ICC by self-crossing,respectively.The ICC can serve as ornamental fish and edible fish.They are also ideal parents to produce triploids by mating with tetraploids.The new ICC plays an important role in biological evolution and fish genetic breeding.
