To establish a standard system for geneticstudies on sheath blight resistance, a field testwas conducted at the experimental farm ofYangzhou University to compare several pro-cedures for inoculating rice plants with R...To establish a standard system for geneticstudies on sheath blight resistance, a field testwas conducted at the experimental farm ofYangzhou University to compare several pro-cedures for inoculating rice plants with R.solani Kuhn (RH9). The varieties used wereJasmine 85, Teqing (resistant or moderatelyresistant), and Lemont (susceptible). They展开更多
Previously we isolated three Fusarium strains(a F.sacchari strain namely GXUF-1,and another two F.commune strains namely GXUF-2 and GXUF-3),and we verified that GXUF-3 was able to cause sugarcane root rot to the chewi...Previously we isolated three Fusarium strains(a F.sacchari strain namely GXUF-1,and another two F.commune strains namely GXUF-2 and GXUF-3),and we verified that GXUF-3 was able to cause sugarcane root rot to the chewing cane cultivar Badila.Considering that Fusarium spp.are a group of widely distributed fungal pathogens,we tested whether these three Fusarium isolates were able to cause root rot to Badila as well as sugar-making cane cultivar(Guitang42),using a suitable inoculation method established based on infection assays using Badila.We found that the three Fusarium strains were able to cause root rot symptoms to both Badila and Guitang42,to different extents.To better investigate the potential pathogenicity mechanisms,we performed Illumina high-throughput sequencing and analyzed the whole genomic sequence data of these three Fusarium strains.The results reveal that the assembly sizes of the three Fusarium strains were in a range of 44.7-48.2 Mb,with G+C contents of 48.0-48.5%,and 14,154-15,175 coding genes.The coding genes were annotated by multiple public databases,and potential pathogenic genes were predicted using proprietary databases(such as PHI,DFVF,CAZy,etc.).Furthermore,based on evolutionary analysis of the coding sequence,we found that contraction and expansion of gene families occurred in the three Fusarium strains.Overall,our results suggest a potential risk that the root rot disease may occur to the sugar-making canes although it was initially spotted from fruit cane,and provide clues to understand the pathogenic mechanisms of Fusarium spp.causing sugarcane root rot.展开更多
文摘To establish a standard system for geneticstudies on sheath blight resistance, a field testwas conducted at the experimental farm ofYangzhou University to compare several pro-cedures for inoculating rice plants with R.solani Kuhn (RH9). The varieties used wereJasmine 85, Teqing (resistant or moderatelyresistant), and Lemont (susceptible). They
基金supported by National Natural Science Foundation of China(U23A20148)Key Projects of Guangzhou Science and Technology Plan(201904020010)。
文摘Previously we isolated three Fusarium strains(a F.sacchari strain namely GXUF-1,and another two F.commune strains namely GXUF-2 and GXUF-3),and we verified that GXUF-3 was able to cause sugarcane root rot to the chewing cane cultivar Badila.Considering that Fusarium spp.are a group of widely distributed fungal pathogens,we tested whether these three Fusarium isolates were able to cause root rot to Badila as well as sugar-making cane cultivar(Guitang42),using a suitable inoculation method established based on infection assays using Badila.We found that the three Fusarium strains were able to cause root rot symptoms to both Badila and Guitang42,to different extents.To better investigate the potential pathogenicity mechanisms,we performed Illumina high-throughput sequencing and analyzed the whole genomic sequence data of these three Fusarium strains.The results reveal that the assembly sizes of the three Fusarium strains were in a range of 44.7-48.2 Mb,with G+C contents of 48.0-48.5%,and 14,154-15,175 coding genes.The coding genes were annotated by multiple public databases,and potential pathogenic genes were predicted using proprietary databases(such as PHI,DFVF,CAZy,etc.).Furthermore,based on evolutionary analysis of the coding sequence,we found that contraction and expansion of gene families occurred in the three Fusarium strains.Overall,our results suggest a potential risk that the root rot disease may occur to the sugar-making canes although it was initially spotted from fruit cane,and provide clues to understand the pathogenic mechanisms of Fusarium spp.causing sugarcane root rot.
