To reveal the saddle-type dose effect relationship, we propose a radiation mutagenesis model based on maize nutrition difference resulting from heavy ion ~7Li radiation. Through irradiation mutagenesis, apparent trait...To reveal the saddle-type dose effect relationship, we propose a radiation mutagenesis model based on maize nutrition difference resulting from heavy ion ~7Li radiation. Through irradiation mutagenesis, apparent trait selection, amino acids and fatty acids content determination, and modeling, dynamic evolution from microscopic damage and repair initiation to the final macroscopic biological effects are considered simultaneously. The results show that the steady state nature is independent of evolution time and only relates to different radiation doses.Heavy ion ~7Li radiation could effectively cause maize phenotypic variation and could improve nutritional quality.This model not only gives a good fit to the experimental results on most types of amino acids and fatty acids, but also offers an adequate explanation of the experimental phenomenon underlying the saddle-type bimodal dose effect. By combining experimental results with theoretical analyses, we suggest that the synergy of the stimulus effect and momentum transfer is the main cause of the saddle-type dose effect bimodal curve. This provides an effective strategy for conducting maize germplasm innovation.展开更多
Rice（Oryza sativa L.）, a tropical and subtropical crop, is susceptible to low temperature stress during seedling, booting, and flowering stages, which leads to lower grain quality levels and decreasing rice yields. ...Rice（Oryza sativa L.）, a tropical and subtropical crop, is susceptible to low temperature stress during seedling, booting, and flowering stages, which leads to lower grain quality levels and decreasing rice yields. Cold tolerance is affected by multiple genetic factors in rice, and the complex genetic mechanisms associated with chilling stress tolerance remain unclear. Here, we detected seven quantitative trait loci（QTLs） for cold tolerance at booting stage and identified one cold tolerant line, SIL157, in an introgression line population derived from a cross between the indica variety Guichao 2, as the recipient, and Dongxiang common wild rice, as the donor. When compared with Guichao 2, SIL157 showed a stronger cold tolerance during different growth stages. Through an integrated strategy that combined QTL-mapping with expression profile analysis, six candidate genes, which were up-regulated under chilling stress at the seedling and booting developmental stages, were studied. The results may help in understanding cold tolerance mechanisms and in using beneficial alleles from wild rice to improve the cold tolerance of rice cultivars through molecular marker-assisted selection.展开更多
Global food production is a major challenge currently facing humanity,and the primary way to secure global food security is to increase crop yields and enhance nutritional value.Heterosis utilization by crossing of tw...Global food production is a major challenge currently facing humanity,and the primary way to secure global food security is to increase crop yields and enhance nutritional value.Heterosis utilization by crossing of two different homozygous inbred lines has long been applied to increase crop yield.Doubled haploid(DH)technology greatly shortens the breeding process of homozygous lines by up to 3-5 years.In recent years,new haploid induction(HI)techniques based on genes such as DOMAIN OF UNKNOWN FUNCTION 679 membrane protein(DMP)and CENTROMERIC HISTONE3(CENH3)were exciting progresses in generating DHs in a more efficient and simple way(Jiang et al.,2022;Qu et al.,2024).展开更多
基金Supported by the National Natural Science Foundation of China under Grant No.11735006the Hebei Natural Science Foundation of under Grant No.B2014209314the Hebei Science and Technology Research Project of Higher Education under Grant No.ZD2017023
文摘To reveal the saddle-type dose effect relationship, we propose a radiation mutagenesis model based on maize nutrition difference resulting from heavy ion ~7Li radiation. Through irradiation mutagenesis, apparent trait selection, amino acids and fatty acids content determination, and modeling, dynamic evolution from microscopic damage and repair initiation to the final macroscopic biological effects are considered simultaneously. The results show that the steady state nature is independent of evolution time and only relates to different radiation doses.Heavy ion ~7Li radiation could effectively cause maize phenotypic variation and could improve nutritional quality.This model not only gives a good fit to the experimental results on most types of amino acids and fatty acids, but also offers an adequate explanation of the experimental phenomenon underlying the saddle-type bimodal dose effect. By combining experimental results with theoretical analyses, we suggest that the synergy of the stimulus effect and momentum transfer is the main cause of the saddle-type dose effect bimodal curve. This provides an effective strategy for conducting maize germplasm innovation.
基金supported by the National Natural Science Foundation of China(31371585 and 30971755)the Beijing Youth Talent,China(31056102)
文摘Rice（Oryza sativa L.）, a tropical and subtropical crop, is susceptible to low temperature stress during seedling, booting, and flowering stages, which leads to lower grain quality levels and decreasing rice yields. Cold tolerance is affected by multiple genetic factors in rice, and the complex genetic mechanisms associated with chilling stress tolerance remain unclear. Here, we detected seven quantitative trait loci（QTLs） for cold tolerance at booting stage and identified one cold tolerant line, SIL157, in an introgression line population derived from a cross between the indica variety Guichao 2, as the recipient, and Dongxiang common wild rice, as the donor. When compared with Guichao 2, SIL157 showed a stronger cold tolerance during different growth stages. Through an integrated strategy that combined QTL-mapping with expression profile analysis, six candidate genes, which were up-regulated under chilling stress at the seedling and booting developmental stages, were studied. The results may help in understanding cold tolerance mechanisms and in using beneficial alleles from wild rice to improve the cold tolerance of rice cultivars through molecular marker-assisted selection.
基金supported by grants from the National Key R&D Program of China(2023YFD1201501)the Key Technology R&D Program of Jiangsu Province(BE2023366)+1 种基金the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS)the China Agriculture Research System of MOF and MARA(CARS-23).
文摘Global food production is a major challenge currently facing humanity,and the primary way to secure global food security is to increase crop yields and enhance nutritional value.Heterosis utilization by crossing of two different homozygous inbred lines has long been applied to increase crop yield.Doubled haploid(DH)technology greatly shortens the breeding process of homozygous lines by up to 3-5 years.In recent years,new haploid induction(HI)techniques based on genes such as DOMAIN OF UNKNOWN FUNCTION 679 membrane protein(DMP)and CENTROMERIC HISTONE3(CENH3)were exciting progresses in generating DHs in a more efficient and simple way(Jiang et al.,2022;Qu et al.,2024).
