【Objective】This study aimed to establish a quintuple PCR method for rapid and simultaneous detection of Ralstonia solanacearum,Fusarium spp.,Pectobacterium spp.,Enterobacter spp.,and Pythium spp.,which provided tech...【Objective】This study aimed to establish a quintuple PCR method for rapid and simultaneous detection of Ralstonia solanacearum,Fusarium spp.,Pectobacterium spp.,Enterobacter spp.,and Pythium spp.,which provided technical support for early diagnosis of various soil-borne diseases on ginger.【Method】For five types of soil-borne pathogens causing ginger bacterial wilt and rhizome rot,specific primer combinations were designed and screened,the optimal quintuple reaction system was established by exploring optimal primer concentrations,annealing temperature,and sensitivity,and was applied to detect field plant samples to verify its utility.【Result】Specific primers pairs Rs1F/Rs1R,En1F/En1R,and Py1F/Py1R were designed according to flic gene of Ralstonia solanacearum,rpoB gene of Enterobacter spp.,and 18S rDNA of Pythium spp.,and combined with reported Fusarium spp.specific primers Fu3/Fu4 and specific primers 23SPecF/23SPecR of Pectobacterium spp.,a quintuple PCR reaction system for ginger soil-borne pathogens has been established(25.00μL):above primer dosage was 1.20,0.20,0.60,1.60,and 0.15μL respectively;2×PCR Mix 12.50μL;DNA templates of different pathogens were 1.00μL each;added ddH_(2)O to 25.00μL.Annealing temperature was optimized to 55.4℃.The specific fragments with sizes of 516,370,266,207,and 159 bp could be amplified simultaneously in the established quintuple PCR system,and the detection limit of this system for Ralstonia solanacearum,Enterobacter spp.and Pythium spp.reached 10^(-1)pg/μL,for Fusarium spp.and Pectobacterium spp.was 1 pg/μL,and for detecting five pathogens simultaneously was 10^(3)pg/μL.The multiplex PCR system established in this study could successfully detect the diseased plant samples from the field.【Conclusion】The quintuple PCR system established is able to rapid ly and accurately detect Ralstonia solanacearum,Fusarium spp.,Pectobacterium spp.,Enterobacter spp.,and Pythium spp.,which provides a useful tool for timely diagnosis and epidemic monitoring of various soil-borne diseases of ginger.展开更多
The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a mol...The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a molding process,which enables the batch detection of pathogens.It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction,elution,and amplification are completed entirely within the chip.This chip can extract nucleic acids from samples in just 10 min,achieving an extraction efficiency comparable to that of traditional in-tube methods.An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination.This approach allows for the simultaneous detection of 21 common respiratory pathogens,with a detection limit of 10 copies per reaction.Furthermore,applications involving clinical samples demonstrate significant practicality.Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips,this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity.展开更多
In the field of smart agriculture,accurate and efficient object detection technology is crucial for automated crop management.A particularly challenging task in this domain is small object detection,such as the identi...In the field of smart agriculture,accurate and efficient object detection technology is crucial for automated crop management.A particularly challenging task in this domain is small object detection,such as the identification of immature fruits or early stage disease spots.These objects pose significant difficulties due to their small pixel coverage,limited feature information,substantial scale variations,and high susceptibility to complex background interference.These challenges frequently result in inadequate accuracy and robustness in current detection models.This study addresses two critical needs in the cashew cultivation industry—fruitmaturity and anthracnose detection—by proposing an improved YOLOv11-NSDDil model.The method introduces three key technological innovations:(1)The SDDil module is designed and integrated into the backbone network.This module combines depthwise separable convolution with the SimAM attention mechanism to expand the receptive field and enhance contextual semantic capture at a low computational cost,effectively alleviating the feature deficiency problem caused by limited pixel coverage of small objects.Simultaneously,the SDmodule dynamically enhances discriminative features and suppresses background noise,significantly improving the model’s feature discrimination capability in complex environments;(2)The introduction of the DynamicScalSeq-Zoom_cat neck network,significantly improving multi-scale feature fusion;and(3)The optimization of the Minimum Point Distance Intersection over Union(MPDIoU)loss function,which enhances bounding box localization accuracy byminimizing vertex distance.Experimental results on a self-constructed cashew dataset containing 1123 images demonstrate significant performance improvements in the enhanced model:mAP50 reaches 0.825,a 4.6% increase compared to the originalYOLOv11;mAP50-95 improves to 0.624,a 6.5% increase;and recall rises to 0.777,a 2.4%increase.This provides a reliable technical solution for intelligent quality inspection of agricultural products and holds broad application prospects.展开更多
Small object detection has been a focus of attention since the emergence of deep learning-based object detection.Although classical object detection frameworks have made significant contributions to the development of...Small object detection has been a focus of attention since the emergence of deep learning-based object detection.Although classical object detection frameworks have made significant contributions to the development of object detection,there are still many issues to be resolved in detecting small objects due to the inherent complexity and diversity of real-world visual scenes.In particular,the YOLO(You Only Look Once)series of detection models,renowned for their real-time performance,have undergone numerous adaptations aimed at improving the detection of small targets.In this survey,we summarize the state-of-the-art YOLO-based small object detection methods.This review presents a systematic categorization of YOLO-based approaches for small-object detection,organized into four methodological avenues,namely attention-based feature enhancement,detection-head optimization,loss function,and multi-scale feature fusion strategies.We then examine the principal challenges addressed by each category.Finally,we analyze the performance of thesemethods on public benchmarks and,by comparing current approaches,identify limitations and outline directions for future research.展开更多
The method for malware detection based on Application Programming Interface(API)call sequences,as a primary research focus within dynamic detection technologies,currently lacks attention to subsequences of API calls,t...The method for malware detection based on Application Programming Interface(API)call sequences,as a primary research focus within dynamic detection technologies,currently lacks attention to subsequences of API calls,the variety of API call types,and the length of sequences.This oversight leads to overly complex call sequences.To address this issue,a dynamic malware detection approach based on multiple subsequences is proposed.Initially,APIs are remapped and encoded,with the introduction of percentile lengths to process sequences.Subsequently,a combination of One-Dimensional Convolutional Neural Network(1D-CNN)and Bidirectional Long Short-Term Memory(Bi-LSTM)networks,along with an attention mechanism,is employed to extract features from subsequences of varying lengths for feature fusion and classification.Experiments conducted on two widely used public API-based datasets,namelyMalBehavD-V1 and Alibaba Cloud,demonstrate that the proposedmethod reduces the number of API call types by approximately 20%compared to representative deep learning–based API sequence detection methods,while achieving a peak accuracy of 98.70%.Additionally,experimental results indicate that sequence length at the 95th percentile represents the optimal solution that balances classification performance and computational efficiency.展开更多
Aiming at the scale adaptation of automatic driving target detection algorithms in low illumination environments and the shortcomings in target occlusion processing,this paper proposes a YOLO-LKSDS automatic driving d...Aiming at the scale adaptation of automatic driving target detection algorithms in low illumination environments and the shortcomings in target occlusion processing,this paper proposes a YOLO-LKSDS automatic driving detection model.Firstly,the Contrast-Limited Adaptive Histogram Equalisation(CLAHE)image enhancement algorithm is improved to increase the image contrast and enhance the detailed features of the target;then,on the basis of the YOLOv5 model,the Kmeans++clustering algorithm is introduced to obtain a suitable anchor frame,and SPPELAN spatial pyramid pooling is improved to enhance the accuracy and robustness of the model for multi-scale target detection.Finally,an improved SEAM(Separated and Enhancement Attention Module)attention mechanism is combined with the DIOU-NMS algorithm to optimize the model’s performance when dealing with occlusion and dense scenes.Compared with the original model,the improved YOLO-LKSDS model achieves a 13.3%improvement in accuracy,a 1.7%improvement in mAP,and 240,000 fewer parameters on the BDD100K dataset.In order to validate the generalization of the improved algorithm,we selected the KITTI dataset for experimentation,which shows that YOLOv5’s accuracy improves by 21.1%,recall by 36.6%,and mAP50 by 29.5%,respectively,on the KITTI dataset.The deployment of this paper’s algorithm is verified by an edge computing platform,where the average speed of detection reaches 24.4 FPS while power consumption remains below 9 W,demonstrating high real-time capability and energy efficiency.展开更多
Camouflaged Object Detection(COD)aims to identify objects that share highly similar patterns—such as texture,intensity,and color—with their surrounding environment.Due to their intrinsic resemblance to the backgroun...Camouflaged Object Detection(COD)aims to identify objects that share highly similar patterns—such as texture,intensity,and color—with their surrounding environment.Due to their intrinsic resemblance to the background,camouflaged objects often exhibit vague boundaries and varying scales,making it challenging to accurately locate targets and delineate their indistinct edges.To address this,we propose a novel camouflaged object detection network called Edge-Guided and Multi-scale Fusion Network(EGMFNet),which leverages edge-guided multi-scale integration for enhanced performance.The model incorporates two innovative components:a Multi-scale Fusion Module(MSFM)and an Edge-Guided Attention Module(EGA).These designs exploit multi-scale features to uncover subtle cues between candidate objects and the background while emphasizing camouflaged object boundaries.Moreover,recognizing the rich contextual information in fused features,we introduce a Dual-Branch Global Context Module(DGCM)to refine features using extensive global context,thereby generatingmore informative representations.Experimental results on four benchmark datasets demonstrate that EGMFNet outperforms state-of-the-art methods across five evaluation metrics.Specifically,on COD10K,our EGMFNet-P improves F_(β)by 4.8 points and reduces mean absolute error(MAE)by 0.006 compared with ZoomNeXt;on NC4K,it achieves a 3.6-point increase in F_(β).OnCAMO and CHAMELEON,it obtains 4.5-point increases in F_(β),respectively.These consistent gains substantiate the superiority and robustness of EGMFNet.展开更多
Deep learning has become integral to robotics,particularly in tasks such as robotic grasping,where objects often exhibit diverse shapes,textures,and physical properties.In robotic grasping tasks,due to the diverse cha...Deep learning has become integral to robotics,particularly in tasks such as robotic grasping,where objects often exhibit diverse shapes,textures,and physical properties.In robotic grasping tasks,due to the diverse characteristics of the targets,frequent adjustments to the network architecture and parameters are required to avoid a decrease in model accuracy,which presents a significant challenge for non-experts.Neural Architecture Search(NAS)provides a compelling method through the automated generation of network architectures,enabling the discovery of models that achieve high accuracy through efficient search algorithms.Compared to manually designed networks,NAS methods can significantly reduce design costs,time expenditure,and improve model performance.However,such methods often involve complex topological connections,and these redundant structures can severely reduce computational efficiency.To overcome this challenge,this work puts forward a robotic grasp detection framework founded on NAS.The method automatically designs a lightweight network with high accuracy and low topological complexity,effectively adapting to the target object to generate the optimal grasp pose,thereby significantly improving the success rate of robotic grasping.Additionally,we use Class Activation Mapping(CAM)as an interpretability tool,which captures sensitive information during the perception process through visualized results.The searched model achieved competitive,and in some cases superior,performance on the Cornell and Jacquard public datasets,achieving accuracies of 98.3%and 96.8%,respectively,while sustaining a detection speed of 89 frames per second with only 0.41 million parameters.To further validate its effectiveness beyond benchmark evaluations,we conducted real-world grasping experiments on a UR5 robotic arm,where the model demonstrated reliable performance across diverse objects and high grasp success rates,thereby confirming its practical applicability in robotic manipulation tasks.展开更多
To solve the false detection and missed detection problems caused by various types and sizes of defects in the detection of steel surface defects,similar defects and background features,and similarities between differ...To solve the false detection and missed detection problems caused by various types and sizes of defects in the detection of steel surface defects,similar defects and background features,and similarities between different defects,this paper proposes a lightweight detection model named multiscale edge and squeeze-and-excitation attention detection network(MSESE),which is built upon the You Only Look Once version 11 nano(YOLOv11n).To address the difficulty of locating defect edges,we first propose an edge enhancement module(EEM),apply it to the process of multiscale feature extraction,and then propose a multiscale edge enhancement module(MSEEM).By obtaining defect features from different scales and enhancing their edge contours,the module uses the dual-domain selection mechanism to effectively focus on the important areas in the image to ensure that the feature images have richer information and clearer contour features.By fusing the squeeze-and-excitation attention mechanism with the EEM,we obtain a lighter module that can enhance the representation of edge features,which is named the edge enhancement module with squeeze-and-excitation attention(EEMSE).This module was subsequently integrated into the detection head.The enhanced detection head achieves improved edge feature enhancement with reduced computational overhead,while effectively adjusting channel-wise importance and further refining feature representation.Experiments on the NEU-DET dataset show that,compared with the original YOLOv11n,the improved model achieves improvements of 4.1%and 2.2%in terms of mAP@0.5 and mAP@0.5:0.95,respectively,and the GFLOPs value decreases from the original value of 6.4 to 6.2.Furthermore,when compared to current mainstream models,Mamba-YOLOT and RTDETR-R34,our method achieves superior performance with 6.5%and 8.9%higher mAP@0.5,respectively,while maintaining a more compact parameter footprint.These results collectively validate the effectiveness and efficiency of our proposed approach.展开更多
Community detection is a fundamental problem in network analysis for identifying densely connected node clusters,with successful applications in diverse fields like social networks,recommendation systems,biology,and c...Community detection is a fundamental problem in network analysis for identifying densely connected node clusters,with successful applications in diverse fields like social networks,recommendation systems,biology,and cyberattack detection.Overlapping community detection refers to the case of a node belonging to multiple communities simultaneously,which is a much more meaningful and challenging task.Graph representation learning with Evolutionary Computation has been studied well in overlapping community detection to deal with complex network structures and characteristics.However,most of them focus on searching the entire solution space,which can be inefficient and lead to inadequate results.To overcome the problem,a structural feature node extraction method is first proposed that can effectively map a network into a structural embedding space.Thus,nodes within the network are classified into hierarchical levels based on their structural feature strength,and only nodes with relatively high strength are considered in subsequent search steps to reduce the search space.Then,a maximal-clique representation method is employed on the given vertex set to identify overlapping nodes.A hybrid clique-based multi-objective evolutionary algorithmwith decomposition method is designed to address cliques and the remaining unexplored nodes separately.The number of communities generated with this allocation method is closer to the actual partition count with high division quality.Experimental results on nine usually used real-world networks,five synthetic networks,and two large-scale networks demonstrate the effectiveness of the proposed methodology in terms of community quality and algorithmic efficiency,compared to traditional,MOEA-based,and graph embedding-based community detection algorithms.展开更多
At inference time,deep neural networks are susceptible to backdoor attacks,which can produce attackercontrolled outputs when inputs contain carefully crafted triggers.Existing defense methods often focus on specific a...At inference time,deep neural networks are susceptible to backdoor attacks,which can produce attackercontrolled outputs when inputs contain carefully crafted triggers.Existing defense methods often focus on specific attack types or incur high costs,such as data cleaning or model fine-tuning.In contrast,we argue that it is possible to achieve effective and generalizable defense without removing triggers or incurring high model-cleaning costs.Fromthe attacker’s perspective and based on characteristics of vulnerable neuron activation anomalies,we propose an Adaptive Feature Injection(AFI)method for black-box backdoor detection.AFI employs a pre-trained image encoder to extract multi-level deep features and constructs a dynamic weight fusionmechanism for precise identification and interception of poisoned samples.Specifically,we select the control samples with the largest feature differences fromthe clean dataset via feature-space analysis,and generate blended sample pairs with the test sample using dynamic linear interpolation.The detection statistic is computed by measuring the divergence G(x)in model output responses.We systematically evaluate the effectiveness of AFI against representative backdoor attacks,including BadNets,Blend,WaNet,and IAB,on three benchmark datasets:MNIST,CIFAR-10,and ImageNet.Experimental results show that AFI can effectively detect poisoned samples,achieving average detection rates of 95.20%,94.15%,and 86.49%on these datasets,respectively.Compared with existing methods,AFI demonstrates strong cross-domain generalization ability and robustness to unknown attacks.展开更多
With the continuous development of artificial intelligence and computer vision technology,numerous deep learning-based lane line detection methods have emerged.DeepLabv3+,as a classic semantic segmentation model,has f...With the continuous development of artificial intelligence and computer vision technology,numerous deep learning-based lane line detection methods have emerged.DeepLabv3+,as a classic semantic segmentation model,has found widespread application in the field of lane line detection.However,the accuracy of lane line segmentation is often compromised by factors such as changes in lighting conditions,occlusions,and wear and tear on the lane lines.Additionally,DeepLabv3+suffers from high memory consumption and challenges in deployment on embedded platforms.To address these issues,this paper proposes a lane line detection method for complex road scenes based on DeepLabv3+and MobileNetV4(MNv4).First,the lightweight MNv4 is adopted as the backbone network,and the standard convolutions in ASPP are replaced with depthwise separable convolutions.Second,a polarization attention mechanism is introduced after the ASPP module to enhance the model’s generalization capability.Finally,the Simple Linear Iterative Clustering(SLIC)superpixel segmentation algorithmis employed to preserve lane line edge information.MNv4-DeepLabv3+was tested on the TuSimple and CULane datasets.On the TuSimple dataset,theMean Intersection over Union(MIoU)and Mean Pixel Accuracy(mPA)improved by 1.01%and 7.49%,respectively.On the CULane dataset,MIoU andmPA increased by 3.33%and 7.74%,respectively.Thenumber of parameters decreased from 54.84 to 3.19 M.Experimental results demonstrate that MNv4-DeepLabv3+significantly optimizes model parameter count and enhances segmentation accuracy.展开更多
The threat posed by space debris to space security is continuously increasing.Optical observation is the main detection method for space debris,but the variety of observation geometries in available measurement datase...The threat posed by space debris to space security is continuously increasing.Optical observation is the main detection method for space debris,but the variety of observation geometries in available measurement datasets is limited.Therefore,simulations are required to supplement observational data.Hardware-in-the-loop(HIL)simulations can provide high-quality simulated optical detection data at a reasonable cost,but existing hardware-in-the-loop methods are only adapted to simple motion scenarios.To extend the simulation ability to complex space motion scenarios,here we propose an optical hardware-in-the-loop space debris simulation method,relying on dynamic detection scenarios,that uses a collaborative scenario-modality-feature simulation scheme to simulate variable observation geometries and to obtain sequential space debris simulation data covering a variety of modalities and scenarios.We apply the proposed space debris detection method to ground-based and space-based simulation experiments and analyze target features within the simulated detection data to demonstrate the usefulness of such simulations.Our simulation method is applicable to space debris optical detection under diverse observation conditions and to multidimensional space debris feature characterization.展开更多
In modern industrial production,foreign object detection in complex environments is crucial to ensure product quality and production safety.Detection systems based on deep-learning image processing algorithms often fa...In modern industrial production,foreign object detection in complex environments is crucial to ensure product quality and production safety.Detection systems based on deep-learning image processing algorithms often face challenges with handling high-resolution images and achieving accurate detection against complex backgrounds.To address these issues,this study employs the PatchCore unsupervised anomaly detection algorithm combined with data augmentation techniques to enhance the system’s generalization capability across varying lighting conditions,viewing angles,and object scales.The proposed method is evaluated in a complex industrial detection scenario involving the bogie of an electric multiple unit(EMU).A dataset consisting of complex backgrounds,diverse lighting conditions,and multiple viewing angles is constructed to validate the performance of the detection system in real industrial environments.Experimental results show that the proposed model achieves an average area under the receiver operating characteristic curve(AUROC)of 0.92 and an average F1 score of 0.85.Combined with data augmentation,the proposed model exhibits improvements in AUROC by 0.06 and F1 score by 0.03,demonstrating enhanced accuracy and robustness for foreign object detection in complex industrial settings.In addition,the effects of key factors on detection performance are systematically analyzed,providing practical guidance for parameter selection in real industrial applications.展开更多
With the deep integration of smart manufacturing and IoT technologies,higher demands are placed on the intelligence and real-time performance of industrial equipment fault detection.For industrial fans,base bolt loose...With the deep integration of smart manufacturing and IoT technologies,higher demands are placed on the intelligence and real-time performance of industrial equipment fault detection.For industrial fans,base bolt loosening faults are difficult to identify through conventional spectrum analysis,and the extreme scarcity of fault data leads to limited training datasets,making traditional deep learning methods inaccurate in fault identification and incapable of detecting loosening severity.This paper employs Bayesian Learning by training on a small fault dataset collected from the actual operation of axial-flow fans in a factory to obtain posterior distribution.This method proposes specific data processing approaches and a configuration of Bayesian Convolutional Neural Network(BCNN).It can effectively improve the model’s generalization ability.Experimental results demonstrate high detection accuracy and alignment with real-world applications,offering practical significance and reference value for industrial fan bolt loosening detection under data-limited conditions.展开更多
With more and more IoT terminals being deployed in various power grid business scenarios,terminal reliability has become a practical challenge that threatens the current security protection architecture.Most IoT termi...With more and more IoT terminals being deployed in various power grid business scenarios,terminal reliability has become a practical challenge that threatens the current security protection architecture.Most IoT terminals have security risks and vulnerabilities,and limited resources make it impossible to deploy costly security protection methods on the terminal.In order to cope with these problems,this paper proposes a lightweight trust evaluation model TCL,which combines three network models,TCN,CNN,and LSTM,with stronger feature extraction capability and can score the reliability of the device by periodically analyzing the traffic behavior and activity logs generated by the terminal device,and the trust evaluation of the terminal’s continuous behavior can be achieved by combining the scores of different periods.After experiments,it is proved that TCL can effectively use the traffic behaviors and activity logs of terminal devices for trust evaluation and achieves F1-score of 95.763,94.456,99.923,and 99.195 on HDFS,BGL,N-BaIoT,and KDD99 datasets,respectively,and the size of TCL is only 91KB,which can achieve similar or better performance than CNN-LSTM,RobustLog and other methods with less computational resources and storage space.展开更多
Herein,a reusable and portable surface-enhanced Raman spectroscopy(SERS)sandpaper was successfully synthesized for the sensitive detection of S-fenvalerate in foods.Commercial sandpapers were decorated with Ag@SiO2@Au...Herein,a reusable and portable surface-enhanced Raman spectroscopy(SERS)sandpaper was successfully synthesized for the sensitive detection of S-fenvalerate in foods.Commercial sandpapers were decorated with Ag@SiO2@Au nanoarrays via a liquid-liquid interface self-assembly method.The capacity of sandpaper to float directly on the cyclohexane-water interface allows nanoarrays to be formed directly on it,thereby minimizing stacking issues typically associated with nanoarray assemblies and significantly enhancing the sensitivity of S-fenvalerate detection.Moreover,the SERS sandpaper was reusable and portable due to its strong adhesion of the nanoarrays.Under optimized testing conditions,the developed SERS sandpaper method was capable of detecting S-fenvalerate,demonstrating a strong linear response within a concentration range of 10^(–7)–10^(3)μmol/L,with a limit of detection of 1.92×10^(−8)μmol/L.The analysis of spiked food samples containing S-fenvalerate using the developed SERS sandpaper afforded excellent recoveries(92.2%−109.7%).Additionally,the SERS sandpaper was successfully applied to quantify S-fenvalerate in real food samples,with results consistent with analyses conducted using gas chromatography.展开更多
Early fault detection for spiral bevel gears is crucial to ensure normal operation and prevent accidents.The harmonic components,excited by the time-varying mesh stiffness,always appear in measured vibration signal.Ho...Early fault detection for spiral bevel gears is crucial to ensure normal operation and prevent accidents.The harmonic components,excited by the time-varying mesh stiffness,always appear in measured vibration signal.How to extract the periodical impulses that indicate gear localized fault buried in the intensive noise and interfered by harmonics is a challenging task.In this paper,a novel Periodical Sparse-Assisted Decoupling(PSAD)method is proposed as an optimization problem to extract fault feature from noisy vibration signal.The PSAD method decouples the impulsive fault feature and harmonic components based on the sparse representation method.The sparsity within and across groups property and the periodicity of the fault feature are incorporated into the regularizer as the prior information.The nonconvex penalty is employed to highlight the sparsity of fault features.Meanwhile,the weight factor based on2norm of each group is constructed to strengthen the amplitude of fault feature.An iterative algorithm with Majorization-Minimization(MM)is derived to solve the optimization problem.Simulation study and experimental analysis confirm the performance of the proposed PSAD method in extracting and enhancing defect impulses from noisy signal.The suggested method surpasses other comparative methods in extracting and enhancing fault features.展开更多
With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a c...With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a convenient and visual technique with low equipment requirements and high sensitivity for the field detection of GM plants is still lacking.On the basis of the existing recombinase polymerase amplification(RPA)technique,we developed a multiplex RPA(multi-RPA)method that can simultaneously detect three transgenic elements,including the cauliflower mosaic virus 35S gene(CaMV35S)promoter,neomycin phosphotransferaseⅡgene(NptⅡ)and hygromycin B phosphotransferase gene(Hyg),thus improving the detection rate.Moreover,we coupled this multi-RPA technique with the CRISPR/Cas12a reporter system,which enabled the detection results to be clearly observed by naked eyes under ultraviolet(UV)light(254 nm;which could be achieved by a portable UV flashlight),therefore establishing a multi-RPA visual detection technique.Compared with the traditional test strip detection method,this multi-RPA-CRISPR/Cas12a technique has the higher specificity,higher sensitivity,wider application range and lower cost.Compared with other polymerase chain reaction(PCR)techniques,it also has the advantages of low equipment requirements and visualization,making it a potentially feasible method for the field detection of GM plants.展开更多
The traditional You Only Look Once(YOLO)series network models often fail to extract satisfactory features for road detection,due to the limited number of defect images in the dataset.Additionally,most open-source road...The traditional You Only Look Once(YOLO)series network models often fail to extract satisfactory features for road detection,due to the limited number of defect images in the dataset.Additionally,most open-source road crack datasets contain idealized cracks that are not suitable for detecting early-stage pavement cracks with fine widths and subtle features.To address these issues,this study collected a large number of original road surface images using road detection vehicles.A large-capacity crack dataset was then constructed,with various shapes of cracks categorized as either cracks or fractures.To improve the training performance of the YOLOv5 algorithm,which showed unsatisfactory results on the original dataset,this study used median filtering to preprocess the crack images.The preprocessed images were combined to form the training set.Moreover,the Coordinate Attention(CA)attention module was integrated to further enhance the model’s training performance.The final detection model achieved a recognition accuracy of 88.9%and a recall rate of 86.1%for detecting cracks.These findings demonstrate that the use of image preprocessing technology and the introduction of the CA attention mechanism can effectively detect early-stage pavement cracks that have low contrast with the background.展开更多
基金National Natural Science Foundation of China(32270237)Guangxi Key Research and Development Plan Project(Guike AB21238002)Basic Scientific Research Project of Guangxi Academy of Agricultural Sciences(Guinongke 2024YP082)。
文摘【Objective】This study aimed to establish a quintuple PCR method for rapid and simultaneous detection of Ralstonia solanacearum,Fusarium spp.,Pectobacterium spp.,Enterobacter spp.,and Pythium spp.,which provided technical support for early diagnosis of various soil-borne diseases on ginger.【Method】For five types of soil-borne pathogens causing ginger bacterial wilt and rhizome rot,specific primer combinations were designed and screened,the optimal quintuple reaction system was established by exploring optimal primer concentrations,annealing temperature,and sensitivity,and was applied to detect field plant samples to verify its utility.【Result】Specific primers pairs Rs1F/Rs1R,En1F/En1R,and Py1F/Py1R were designed according to flic gene of Ralstonia solanacearum,rpoB gene of Enterobacter spp.,and 18S rDNA of Pythium spp.,and combined with reported Fusarium spp.specific primers Fu3/Fu4 and specific primers 23SPecF/23SPecR of Pectobacterium spp.,a quintuple PCR reaction system for ginger soil-borne pathogens has been established(25.00μL):above primer dosage was 1.20,0.20,0.60,1.60,and 0.15μL respectively;2×PCR Mix 12.50μL;DNA templates of different pathogens were 1.00μL each;added ddH_(2)O to 25.00μL.Annealing temperature was optimized to 55.4℃.The specific fragments with sizes of 516,370,266,207,and 159 bp could be amplified simultaneously in the established quintuple PCR system,and the detection limit of this system for Ralstonia solanacearum,Enterobacter spp.and Pythium spp.reached 10^(-1)pg/μL,for Fusarium spp.and Pectobacterium spp.was 1 pg/μL,and for detecting five pathogens simultaneously was 10^(3)pg/μL.The multiplex PCR system established in this study could successfully detect the diseased plant samples from the field.【Conclusion】The quintuple PCR system established is able to rapid ly and accurately detect Ralstonia solanacearum,Fusarium spp.,Pectobacterium spp.,Enterobacter spp.,and Pythium spp.,which provides a useful tool for timely diagnosis and epidemic monitoring of various soil-borne diseases of ginger.
基金supported by grants from the National Key Research and Development Program of China(Nos.2023YFA0915200,2023YFA0915204)the Equipment Research and Development Projects of the Chinese Academy of Sciences(No.PTYQ2024YZ0010)+3 种基金the Science and Technology Commission of Shanghai Municipality Project(No.XTCX-KJ-2024-038)the Natural Science Foundation of Hebei Province of China(No.H2024206249)the Postdoctoral Fellowship Program of CPSF(No.GZC20232838)Science and Technology Commission of Shanghai Municipality(No.22S31901700).
文摘The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a molding process,which enables the batch detection of pathogens.It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction,elution,and amplification are completed entirely within the chip.This chip can extract nucleic acids from samples in just 10 min,achieving an extraction efficiency comparable to that of traditional in-tube methods.An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination.This approach allows for the simultaneous detection of 21 common respiratory pathogens,with a detection limit of 10 copies per reaction.Furthermore,applications involving clinical samples demonstrate significant practicality.Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips,this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity.
基金supported by Hebei North University Doctoral Research Fund Project(No.BSJJ202315)the Youth Research Fund Project of Higher Education Institutions in Hebei Province(No.QN2024146).
文摘In the field of smart agriculture,accurate and efficient object detection technology is crucial for automated crop management.A particularly challenging task in this domain is small object detection,such as the identification of immature fruits or early stage disease spots.These objects pose significant difficulties due to their small pixel coverage,limited feature information,substantial scale variations,and high susceptibility to complex background interference.These challenges frequently result in inadequate accuracy and robustness in current detection models.This study addresses two critical needs in the cashew cultivation industry—fruitmaturity and anthracnose detection—by proposing an improved YOLOv11-NSDDil model.The method introduces three key technological innovations:(1)The SDDil module is designed and integrated into the backbone network.This module combines depthwise separable convolution with the SimAM attention mechanism to expand the receptive field and enhance contextual semantic capture at a low computational cost,effectively alleviating the feature deficiency problem caused by limited pixel coverage of small objects.Simultaneously,the SDmodule dynamically enhances discriminative features and suppresses background noise,significantly improving the model’s feature discrimination capability in complex environments;(2)The introduction of the DynamicScalSeq-Zoom_cat neck network,significantly improving multi-scale feature fusion;and(3)The optimization of the Minimum Point Distance Intersection over Union(MPDIoU)loss function,which enhances bounding box localization accuracy byminimizing vertex distance.Experimental results on a self-constructed cashew dataset containing 1123 images demonstrate significant performance improvements in the enhanced model:mAP50 reaches 0.825,a 4.6% increase compared to the originalYOLOv11;mAP50-95 improves to 0.624,a 6.5% increase;and recall rises to 0.777,a 2.4%increase.This provides a reliable technical solution for intelligent quality inspection of agricultural products and holds broad application prospects.
基金supported in part by the by Chongqing Research Program of Basic Research and Frontier Technology under Grant CSTB2025NSCQ-GPX1309.
文摘Small object detection has been a focus of attention since the emergence of deep learning-based object detection.Although classical object detection frameworks have made significant contributions to the development of object detection,there are still many issues to be resolved in detecting small objects due to the inherent complexity and diversity of real-world visual scenes.In particular,the YOLO(You Only Look Once)series of detection models,renowned for their real-time performance,have undergone numerous adaptations aimed at improving the detection of small targets.In this survey,we summarize the state-of-the-art YOLO-based small object detection methods.This review presents a systematic categorization of YOLO-based approaches for small-object detection,organized into four methodological avenues,namely attention-based feature enhancement,detection-head optimization,loss function,and multi-scale feature fusion strategies.We then examine the principal challenges addressed by each category.Finally,we analyze the performance of thesemethods on public benchmarks and,by comparing current approaches,identify limitations and outline directions for future research.
基金supported by the National Natural Science Foundation of China(62262020)the Graduate Education Innovation Project of Hubei Minzu University(MYK2024025).
文摘The method for malware detection based on Application Programming Interface(API)call sequences,as a primary research focus within dynamic detection technologies,currently lacks attention to subsequences of API calls,the variety of API call types,and the length of sequences.This oversight leads to overly complex call sequences.To address this issue,a dynamic malware detection approach based on multiple subsequences is proposed.Initially,APIs are remapped and encoded,with the introduction of percentile lengths to process sequences.Subsequently,a combination of One-Dimensional Convolutional Neural Network(1D-CNN)and Bidirectional Long Short-Term Memory(Bi-LSTM)networks,along with an attention mechanism,is employed to extract features from subsequences of varying lengths for feature fusion and classification.Experiments conducted on two widely used public API-based datasets,namelyMalBehavD-V1 and Alibaba Cloud,demonstrate that the proposedmethod reduces the number of API call types by approximately 20%compared to representative deep learning–based API sequence detection methods,while achieving a peak accuracy of 98.70%.Additionally,experimental results indicate that sequence length at the 95th percentile represents the optimal solution that balances classification performance and computational efficiency.
基金supported by the Key R&D Program of Shaanxi Province(No.2025CYYBXM-078).
文摘Aiming at the scale adaptation of automatic driving target detection algorithms in low illumination environments and the shortcomings in target occlusion processing,this paper proposes a YOLO-LKSDS automatic driving detection model.Firstly,the Contrast-Limited Adaptive Histogram Equalisation(CLAHE)image enhancement algorithm is improved to increase the image contrast and enhance the detailed features of the target;then,on the basis of the YOLOv5 model,the Kmeans++clustering algorithm is introduced to obtain a suitable anchor frame,and SPPELAN spatial pyramid pooling is improved to enhance the accuracy and robustness of the model for multi-scale target detection.Finally,an improved SEAM(Separated and Enhancement Attention Module)attention mechanism is combined with the DIOU-NMS algorithm to optimize the model’s performance when dealing with occlusion and dense scenes.Compared with the original model,the improved YOLO-LKSDS model achieves a 13.3%improvement in accuracy,a 1.7%improvement in mAP,and 240,000 fewer parameters on the BDD100K dataset.In order to validate the generalization of the improved algorithm,we selected the KITTI dataset for experimentation,which shows that YOLOv5’s accuracy improves by 21.1%,recall by 36.6%,and mAP50 by 29.5%,respectively,on the KITTI dataset.The deployment of this paper’s algorithm is verified by an edge computing platform,where the average speed of detection reaches 24.4 FPS while power consumption remains below 9 W,demonstrating high real-time capability and energy efficiency.
基金financially supported byChongqingUniversity of Technology Graduate Innovation Foundation(Grant No.gzlcx20253267).
文摘Camouflaged Object Detection(COD)aims to identify objects that share highly similar patterns—such as texture,intensity,and color—with their surrounding environment.Due to their intrinsic resemblance to the background,camouflaged objects often exhibit vague boundaries and varying scales,making it challenging to accurately locate targets and delineate their indistinct edges.To address this,we propose a novel camouflaged object detection network called Edge-Guided and Multi-scale Fusion Network(EGMFNet),which leverages edge-guided multi-scale integration for enhanced performance.The model incorporates two innovative components:a Multi-scale Fusion Module(MSFM)and an Edge-Guided Attention Module(EGA).These designs exploit multi-scale features to uncover subtle cues between candidate objects and the background while emphasizing camouflaged object boundaries.Moreover,recognizing the rich contextual information in fused features,we introduce a Dual-Branch Global Context Module(DGCM)to refine features using extensive global context,thereby generatingmore informative representations.Experimental results on four benchmark datasets demonstrate that EGMFNet outperforms state-of-the-art methods across five evaluation metrics.Specifically,on COD10K,our EGMFNet-P improves F_(β)by 4.8 points and reduces mean absolute error(MAE)by 0.006 compared with ZoomNeXt;on NC4K,it achieves a 3.6-point increase in F_(β).OnCAMO and CHAMELEON,it obtains 4.5-point increases in F_(β),respectively.These consistent gains substantiate the superiority and robustness of EGMFNet.
基金funded by Guangdong Basic and Applied Basic Research Foundation(2023B1515120064)National Natural Science Foundation of China(62273097).
文摘Deep learning has become integral to robotics,particularly in tasks such as robotic grasping,where objects often exhibit diverse shapes,textures,and physical properties.In robotic grasping tasks,due to the diverse characteristics of the targets,frequent adjustments to the network architecture and parameters are required to avoid a decrease in model accuracy,which presents a significant challenge for non-experts.Neural Architecture Search(NAS)provides a compelling method through the automated generation of network architectures,enabling the discovery of models that achieve high accuracy through efficient search algorithms.Compared to manually designed networks,NAS methods can significantly reduce design costs,time expenditure,and improve model performance.However,such methods often involve complex topological connections,and these redundant structures can severely reduce computational efficiency.To overcome this challenge,this work puts forward a robotic grasp detection framework founded on NAS.The method automatically designs a lightweight network with high accuracy and low topological complexity,effectively adapting to the target object to generate the optimal grasp pose,thereby significantly improving the success rate of robotic grasping.Additionally,we use Class Activation Mapping(CAM)as an interpretability tool,which captures sensitive information during the perception process through visualized results.The searched model achieved competitive,and in some cases superior,performance on the Cornell and Jacquard public datasets,achieving accuracies of 98.3%and 96.8%,respectively,while sustaining a detection speed of 89 frames per second with only 0.41 million parameters.To further validate its effectiveness beyond benchmark evaluations,we conducted real-world grasping experiments on a UR5 robotic arm,where the model demonstrated reliable performance across diverse objects and high grasp success rates,thereby confirming its practical applicability in robotic manipulation tasks.
基金funded by Ministry of Education Humanities and Social Science Research Project,grant number 23YJAZH034The Postgraduate Research and Practice Innovation Program of Jiangsu Province,grant number SJCX25_17National Computer Basic Education Research Project in Higher Education Institutions,grant number 2024-AFCEC-056,2024-AFCEC-057.
文摘To solve the false detection and missed detection problems caused by various types and sizes of defects in the detection of steel surface defects,similar defects and background features,and similarities between different defects,this paper proposes a lightweight detection model named multiscale edge and squeeze-and-excitation attention detection network(MSESE),which is built upon the You Only Look Once version 11 nano(YOLOv11n).To address the difficulty of locating defect edges,we first propose an edge enhancement module(EEM),apply it to the process of multiscale feature extraction,and then propose a multiscale edge enhancement module(MSEEM).By obtaining defect features from different scales and enhancing their edge contours,the module uses the dual-domain selection mechanism to effectively focus on the important areas in the image to ensure that the feature images have richer information and clearer contour features.By fusing the squeeze-and-excitation attention mechanism with the EEM,we obtain a lighter module that can enhance the representation of edge features,which is named the edge enhancement module with squeeze-and-excitation attention(EEMSE).This module was subsequently integrated into the detection head.The enhanced detection head achieves improved edge feature enhancement with reduced computational overhead,while effectively adjusting channel-wise importance and further refining feature representation.Experiments on the NEU-DET dataset show that,compared with the original YOLOv11n,the improved model achieves improvements of 4.1%and 2.2%in terms of mAP@0.5 and mAP@0.5:0.95,respectively,and the GFLOPs value decreases from the original value of 6.4 to 6.2.Furthermore,when compared to current mainstream models,Mamba-YOLOT and RTDETR-R34,our method achieves superior performance with 6.5%and 8.9%higher mAP@0.5,respectively,while maintaining a more compact parameter footprint.These results collectively validate the effectiveness and efficiency of our proposed approach.
基金supported in part by the National Natural Science Foundation of China under Grants 62473176,62073155,62002137,62106088,and 62206113National Key Laboratory of Ship Structural Safety underGrant 450324300the Postgraduate Research&Practice Innovation Programof Jiangsu Province under Grant KYCX24_2642.
文摘Community detection is a fundamental problem in network analysis for identifying densely connected node clusters,with successful applications in diverse fields like social networks,recommendation systems,biology,and cyberattack detection.Overlapping community detection refers to the case of a node belonging to multiple communities simultaneously,which is a much more meaningful and challenging task.Graph representation learning with Evolutionary Computation has been studied well in overlapping community detection to deal with complex network structures and characteristics.However,most of them focus on searching the entire solution space,which can be inefficient and lead to inadequate results.To overcome the problem,a structural feature node extraction method is first proposed that can effectively map a network into a structural embedding space.Thus,nodes within the network are classified into hierarchical levels based on their structural feature strength,and only nodes with relatively high strength are considered in subsequent search steps to reduce the search space.Then,a maximal-clique representation method is employed on the given vertex set to identify overlapping nodes.A hybrid clique-based multi-objective evolutionary algorithmwith decomposition method is designed to address cliques and the remaining unexplored nodes separately.The number of communities generated with this allocation method is closer to the actual partition count with high division quality.Experimental results on nine usually used real-world networks,five synthetic networks,and two large-scale networks demonstrate the effectiveness of the proposed methodology in terms of community quality and algorithmic efficiency,compared to traditional,MOEA-based,and graph embedding-based community detection algorithms.
基金supported by the National Natural Science Foundation of China Grant(No.61972133)Project of Leading Talents in Science and Technology Innovation for Thousands of People Plan in Henan Province Grant(No.204200510021)the Key Research and Development Plan Special Project of Henan Province Grant(No.241111211400).
文摘At inference time,deep neural networks are susceptible to backdoor attacks,which can produce attackercontrolled outputs when inputs contain carefully crafted triggers.Existing defense methods often focus on specific attack types or incur high costs,such as data cleaning or model fine-tuning.In contrast,we argue that it is possible to achieve effective and generalizable defense without removing triggers or incurring high model-cleaning costs.Fromthe attacker’s perspective and based on characteristics of vulnerable neuron activation anomalies,we propose an Adaptive Feature Injection(AFI)method for black-box backdoor detection.AFI employs a pre-trained image encoder to extract multi-level deep features and constructs a dynamic weight fusionmechanism for precise identification and interception of poisoned samples.Specifically,we select the control samples with the largest feature differences fromthe clean dataset via feature-space analysis,and generate blended sample pairs with the test sample using dynamic linear interpolation.The detection statistic is computed by measuring the divergence G(x)in model output responses.We systematically evaluate the effectiveness of AFI against representative backdoor attacks,including BadNets,Blend,WaNet,and IAB,on three benchmark datasets:MNIST,CIFAR-10,and ImageNet.Experimental results show that AFI can effectively detect poisoned samples,achieving average detection rates of 95.20%,94.15%,and 86.49%on these datasets,respectively.Compared with existing methods,AFI demonstrates strong cross-domain generalization ability and robustness to unknown attacks.
文摘With the continuous development of artificial intelligence and computer vision technology,numerous deep learning-based lane line detection methods have emerged.DeepLabv3+,as a classic semantic segmentation model,has found widespread application in the field of lane line detection.However,the accuracy of lane line segmentation is often compromised by factors such as changes in lighting conditions,occlusions,and wear and tear on the lane lines.Additionally,DeepLabv3+suffers from high memory consumption and challenges in deployment on embedded platforms.To address these issues,this paper proposes a lane line detection method for complex road scenes based on DeepLabv3+and MobileNetV4(MNv4).First,the lightweight MNv4 is adopted as the backbone network,and the standard convolutions in ASPP are replaced with depthwise separable convolutions.Second,a polarization attention mechanism is introduced after the ASPP module to enhance the model’s generalization capability.Finally,the Simple Linear Iterative Clustering(SLIC)superpixel segmentation algorithmis employed to preserve lane line edge information.MNv4-DeepLabv3+was tested on the TuSimple and CULane datasets.On the TuSimple dataset,theMean Intersection over Union(MIoU)and Mean Pixel Accuracy(mPA)improved by 1.01%and 7.49%,respectively.On the CULane dataset,MIoU andmPA increased by 3.33%and 7.74%,respectively.Thenumber of parameters decreased from 54.84 to 3.19 M.Experimental results demonstrate that MNv4-DeepLabv3+significantly optimizes model parameter count and enhances segmentation accuracy.
基金supported by the Special funding project for space debris and Near-Earth Asteroids defense research(KJSP2023020202).
文摘The threat posed by space debris to space security is continuously increasing.Optical observation is the main detection method for space debris,but the variety of observation geometries in available measurement datasets is limited.Therefore,simulations are required to supplement observational data.Hardware-in-the-loop(HIL)simulations can provide high-quality simulated optical detection data at a reasonable cost,but existing hardware-in-the-loop methods are only adapted to simple motion scenarios.To extend the simulation ability to complex space motion scenarios,here we propose an optical hardware-in-the-loop space debris simulation method,relying on dynamic detection scenarios,that uses a collaborative scenario-modality-feature simulation scheme to simulate variable observation geometries and to obtain sequential space debris simulation data covering a variety of modalities and scenarios.We apply the proposed space debris detection method to ground-based and space-based simulation experiments and analyze target features within the simulated detection data to demonstrate the usefulness of such simulations.Our simulation method is applicable to space debris optical detection under diverse observation conditions and to multidimensional space debris feature characterization.
文摘In modern industrial production,foreign object detection in complex environments is crucial to ensure product quality and production safety.Detection systems based on deep-learning image processing algorithms often face challenges with handling high-resolution images and achieving accurate detection against complex backgrounds.To address these issues,this study employs the PatchCore unsupervised anomaly detection algorithm combined with data augmentation techniques to enhance the system’s generalization capability across varying lighting conditions,viewing angles,and object scales.The proposed method is evaluated in a complex industrial detection scenario involving the bogie of an electric multiple unit(EMU).A dataset consisting of complex backgrounds,diverse lighting conditions,and multiple viewing angles is constructed to validate the performance of the detection system in real industrial environments.Experimental results show that the proposed model achieves an average area under the receiver operating characteristic curve(AUROC)of 0.92 and an average F1 score of 0.85.Combined with data augmentation,the proposed model exhibits improvements in AUROC by 0.06 and F1 score by 0.03,demonstrating enhanced accuracy and robustness for foreign object detection in complex industrial settings.In addition,the effects of key factors on detection performance are systematically analyzed,providing practical guidance for parameter selection in real industrial applications.
基金funded by the Zhejiang Provincial Key Science and Technology“LingYan”Project Foundation,grant number 2023C01145Zhejiang Gongshang University Higher Education Research Projects,grant number Xgy22028.
文摘With the deep integration of smart manufacturing and IoT technologies,higher demands are placed on the intelligence and real-time performance of industrial equipment fault detection.For industrial fans,base bolt loosening faults are difficult to identify through conventional spectrum analysis,and the extreme scarcity of fault data leads to limited training datasets,making traditional deep learning methods inaccurate in fault identification and incapable of detecting loosening severity.This paper employs Bayesian Learning by training on a small fault dataset collected from the actual operation of axial-flow fans in a factory to obtain posterior distribution.This method proposes specific data processing approaches and a configuration of Bayesian Convolutional Neural Network(BCNN).It can effectively improve the model’s generalization ability.Experimental results demonstrate high detection accuracy and alignment with real-world applications,offering practical significance and reference value for industrial fan bolt loosening detection under data-limited conditions.
基金supported by National Key R&D Program of China(No.2022YFB3105101).
文摘With more and more IoT terminals being deployed in various power grid business scenarios,terminal reliability has become a practical challenge that threatens the current security protection architecture.Most IoT terminals have security risks and vulnerabilities,and limited resources make it impossible to deploy costly security protection methods on the terminal.In order to cope with these problems,this paper proposes a lightweight trust evaluation model TCL,which combines three network models,TCN,CNN,and LSTM,with stronger feature extraction capability and can score the reliability of the device by periodically analyzing the traffic behavior and activity logs generated by the terminal device,and the trust evaluation of the terminal’s continuous behavior can be achieved by combining the scores of different periods.After experiments,it is proved that TCL can effectively use the traffic behaviors and activity logs of terminal devices for trust evaluation and achieves F1-score of 95.763,94.456,99.923,and 99.195 on HDFS,BGL,N-BaIoT,and KDD99 datasets,respectively,and the size of TCL is only 91KB,which can achieve similar or better performance than CNN-LSTM,RobustLog and other methods with less computational resources and storage space.
基金financially supported by the Key R&D Program of Shandong Province,China(2023CXGC010712).
文摘Herein,a reusable and portable surface-enhanced Raman spectroscopy(SERS)sandpaper was successfully synthesized for the sensitive detection of S-fenvalerate in foods.Commercial sandpapers were decorated with Ag@SiO2@Au nanoarrays via a liquid-liquid interface self-assembly method.The capacity of sandpaper to float directly on the cyclohexane-water interface allows nanoarrays to be formed directly on it,thereby minimizing stacking issues typically associated with nanoarray assemblies and significantly enhancing the sensitivity of S-fenvalerate detection.Moreover,the SERS sandpaper was reusable and portable due to its strong adhesion of the nanoarrays.Under optimized testing conditions,the developed SERS sandpaper method was capable of detecting S-fenvalerate,demonstrating a strong linear response within a concentration range of 10^(–7)–10^(3)μmol/L,with a limit of detection of 1.92×10^(−8)μmol/L.The analysis of spiked food samples containing S-fenvalerate using the developed SERS sandpaper afforded excellent recoveries(92.2%−109.7%).Additionally,the SERS sandpaper was successfully applied to quantify S-fenvalerate in real food samples,with results consistent with analyses conducted using gas chromatography.
基金supported by the National Science Foundationof China(Nos.52305127 and 52475130)。
文摘Early fault detection for spiral bevel gears is crucial to ensure normal operation and prevent accidents.The harmonic components,excited by the time-varying mesh stiffness,always appear in measured vibration signal.How to extract the periodical impulses that indicate gear localized fault buried in the intensive noise and interfered by harmonics is a challenging task.In this paper,a novel Periodical Sparse-Assisted Decoupling(PSAD)method is proposed as an optimization problem to extract fault feature from noisy vibration signal.The PSAD method decouples the impulsive fault feature and harmonic components based on the sparse representation method.The sparsity within and across groups property and the periodicity of the fault feature are incorporated into the regularizer as the prior information.The nonconvex penalty is employed to highlight the sparsity of fault features.Meanwhile,the weight factor based on2norm of each group is constructed to strengthen the amplitude of fault feature.An iterative algorithm with Majorization-Minimization(MM)is derived to solve the optimization problem.Simulation study and experimental analysis confirm the performance of the proposed PSAD method in extracting and enhancing defect impulses from noisy signal.The suggested method surpasses other comparative methods in extracting and enhancing fault features.
基金the Experimental Technology Research Project of Zhejiang University(SYB202138)National Natural Science Foundation of China(32000195)。
文摘With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a convenient and visual technique with low equipment requirements and high sensitivity for the field detection of GM plants is still lacking.On the basis of the existing recombinase polymerase amplification(RPA)technique,we developed a multiplex RPA(multi-RPA)method that can simultaneously detect three transgenic elements,including the cauliflower mosaic virus 35S gene(CaMV35S)promoter,neomycin phosphotransferaseⅡgene(NptⅡ)and hygromycin B phosphotransferase gene(Hyg),thus improving the detection rate.Moreover,we coupled this multi-RPA technique with the CRISPR/Cas12a reporter system,which enabled the detection results to be clearly observed by naked eyes under ultraviolet(UV)light(254 nm;which could be achieved by a portable UV flashlight),therefore establishing a multi-RPA visual detection technique.Compared with the traditional test strip detection method,this multi-RPA-CRISPR/Cas12a technique has the higher specificity,higher sensitivity,wider application range and lower cost.Compared with other polymerase chain reaction(PCR)techniques,it also has the advantages of low equipment requirements and visualization,making it a potentially feasible method for the field detection of GM plants.
基金jointly supported by the National Natural Science Foundation of China(No.52308332)the China Postdoctoral Science Foundation(Grant No.2022M712787).
文摘The traditional You Only Look Once(YOLO)series network models often fail to extract satisfactory features for road detection,due to the limited number of defect images in the dataset.Additionally,most open-source road crack datasets contain idealized cracks that are not suitable for detecting early-stage pavement cracks with fine widths and subtle features.To address these issues,this study collected a large number of original road surface images using road detection vehicles.A large-capacity crack dataset was then constructed,with various shapes of cracks categorized as either cracks or fractures.To improve the training performance of the YOLOv5 algorithm,which showed unsatisfactory results on the original dataset,this study used median filtering to preprocess the crack images.The preprocessed images were combined to form the training set.Moreover,the Coordinate Attention(CA)attention module was integrated to further enhance the model’s training performance.The final detection model achieved a recognition accuracy of 88.9%and a recall rate of 86.1%for detecting cracks.These findings demonstrate that the use of image preprocessing technology and the introduction of the CA attention mechanism can effectively detect early-stage pavement cracks that have low contrast with the background.
