Mass spectrometry-based single-cell proteomics(MS-scP)is attracting tremendous attention because it is now technically feasible to quantify thousands of proteins in minute samples.Since protein amplification is still ...Mass spectrometry-based single-cell proteomics(MS-scP)is attracting tremendous attention because it is now technically feasible to quantify thousands of proteins in minute samples.Since protein amplification is still not possible,technological improvements in MS-scP focus on mini-mizing sample loss while increasing throughput,resolution,and sensitivity,as well as achieving measurement depth,accuracy,and stability comparable to bulk samples.Major advances in MS-sCP have facilitated its application in biological and even medical research.Here,we review the key advancements in MS-SCP technology and discuss the strategies of the typical proteomics workflow to improve MS-SCP analysis from single-cell isolation,sample preparation,and liquid chromatography separation to MS data acquisition and analysis.The review will provide an overall understanding of the development and applications of Ms-scP and inspire more novel ideas regarding the innovation of MS-SCPtechnology.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32371500)the National Key R&D Program of China(Grant No.2022YFA1304500).
文摘Mass spectrometry-based single-cell proteomics(MS-scP)is attracting tremendous attention because it is now technically feasible to quantify thousands of proteins in minute samples.Since protein amplification is still not possible,technological improvements in MS-scP focus on mini-mizing sample loss while increasing throughput,resolution,and sensitivity,as well as achieving measurement depth,accuracy,and stability comparable to bulk samples.Major advances in MS-sCP have facilitated its application in biological and even medical research.Here,we review the key advancements in MS-SCP technology and discuss the strategies of the typical proteomics workflow to improve MS-SCP analysis from single-cell isolation,sample preparation,and liquid chromatography separation to MS data acquisition and analysis.The review will provide an overall understanding of the development and applications of Ms-scP and inspire more novel ideas regarding the innovation of MS-SCPtechnology.
