Silicone rubber/polyacrylate sequential interpenetrating polymer networks(IPNs) were prepared by silicone rubber sheet dipped into the solution composed of different acrylate monomers and benzoyl peroxides(BPOs) for d...Silicone rubber/polyacrylate sequential interpenetrating polymer networks(IPNs) were prepared by silicone rubber sheet dipped into the solution composed of different acrylate monomers and benzoyl peroxides(BPOs) for different time at room temperature and then acrylate polymerized at 80℃for 2 h. The molecular structure and damping properties of sequential IPNs were studied by means of FT-IR and dynamic mechanical analysis(DMA), respectively. The FT-IR spectrum shows that polyacrylate distributes unevenly along the thickness direction of IPNs, i.e. the concentration of polyacrylate decreases from the midst to the surface of the IPNs. The DMA shows that cold crystallization of silicone in the temperature range from -47℃to -30℃is reduced and loss factor of IPNs is improved after interpenetrating with polyacrylate. This suggestes that IPNs can be used as damping materials.展开更多
Interpenetrating polymer networks （IPNs） composed of acrylate-modified polyurethane （PU）/unsaturated polyester （UP） resin via simultaneous polymerization with various component ratios of PU/UP were prepared. The...Interpenetrating polymer networks （IPNs） composed of acrylate-modified polyurethane （PU）/unsaturated polyester （UP） resin via simultaneous polymerization with various component ratios of PU/UP were prepared. The polymerization processes of IPNs were traced through infrared spectrum （IR） techniques, by which the phase separation in systems could be controlled effectively. Results for the morphology and miscibility among multiple phases of IPNs, obtained by transmission electron microscope （TEM） indicated that the domains between two phases were constricted in nanometer scales. The dynamic mechanical thermal analyzer （DMTA） detection results revealed that the loss factor （tanS） and loss modulus （E″） increased with the polyurethane amounts in system, and the peak value in curves of tanδ and E″ appeared toward low temperature ranges. Maximum tanδ values of all samples were above 0.3 in the nearly 50℃ ranges. Also, the mechanical properties of PU/UP IPNs were studied in detail.展开更多
目的探讨轻盈祛浊汤治疗糖尿病肾病(DN)的作用机制。方法利用网络药理学预测轻盈祛浊汤治疗DN的作用机制,并通过分子对接预测活性成分的结合部位。结合网络药理学及分子对接结果,构建DN大鼠模型,2023年1—6月,将60只大鼠分为正常组、模...目的探讨轻盈祛浊汤治疗糖尿病肾病(DN)的作用机制。方法利用网络药理学预测轻盈祛浊汤治疗DN的作用机制,并通过分子对接预测活性成分的结合部位。结合网络药理学及分子对接结果,构建DN大鼠模型,2023年1—6月,将60只大鼠分为正常组、模型组、马来酸依那普利组、轻盈祛浊汤组,每组15只。治疗8周后比较四组大鼠24 h尿微量白蛋白(urinary microalbuminexcretion rate,UAER)、尿素氮、血肌酐水平和钠/葡萄糖协同转运蛋白1(recombinant sodium/glucose cotransporter 1,SGLT1),A1腺苷受体(A1 adenosine receptor,A1AR)蛋白表达水平。结果网络药理学共筛选出轻盈祛浊汤有效成分1114种,作用靶点269个,DN相关靶点2020个,其交集靶点174个。基因本体富集(GO)和基因组的京都百科全书(KEGG)分析得出主要涉及信号转导、炎症反应、细胞凋亡等一系列的生物学反应过程,主要参与丝裂原活化蛋白激酶/核因子κB(mitogen-activated protein kinase/nuclear factor kappa-B,pMAPK/NF-κB)、NOD样受体家族蛋白3/白细胞介素-1β(NOD-like receptor protein 3/interleukin-1β,NLRP3/IL-1β)、白细胞介素6/信号传导和转录激活因子3(interleukin-6/signal transducer and activator of transcription 3,IL-6/STAT3)、肿瘤坏死因子(tumor necrosis factor,TNF)、肿瘤蛋白p53(tumor protein 53,P53)和前列腺素内过氧化物合酶(prostaglandin-endoperoxide synthase 2,PTGS2)等信号通路的调控。分子对接表明,轻盈祛浊汤主要成分与DN靶点的结合活性较强。模型组大鼠24 h UAER[(4539.71±516.03)μg/24 h比(226.59±72.71)μg/24 h]、血肌酐[(85.63±12.96)mL·kg^(-1)·min^(-1)比(0.48±0.12)mL·kg^(-1)·min^(-1)]、SGLT1(1.17±0.07比0.82±0.06)高于正常组,而模型组大鼠尿素氮、A1AR低于正常组(P<0.05)。马来酸依那普利片组大鼠24 h UAER、血肌酐、SGLT1低于模型组,马来酸依那普利片组大鼠尿素氮、A1AR高于模型组(P<0.05)。轻盈祛浊汤组大鼠24 h UAER、血肌酐、SGLT1低于马来酸依那普利片组,而轻盈祛浊汤组大鼠尿素氮、A1AR高于马来酸依那普利片组(P<0.05)。结论轻盈祛浊汤可通过调节pMAPK/NF-κB、NLRP3/IL-1β、IL-6/STAT3、TNF、P53和PTGS2信号通路发挥治疗DN的作用。展开更多
目的运用网络药理学和分子对接技术探究曾江涛经验方(降脂1方)治疗非酒精性脂肪性肝病(NAFLD)的作用机制。方法采用TCMSP和Herb数据库检索降脂1方活性成分及其作用靶点,通过GeneCards、OMIM及DisGeNET数据库收集NAFLD相关靶点,利用Venny...目的运用网络药理学和分子对接技术探究曾江涛经验方(降脂1方)治疗非酒精性脂肪性肝病(NAFLD)的作用机制。方法采用TCMSP和Herb数据库检索降脂1方活性成分及其作用靶点,通过GeneCards、OMIM及DisGeNET数据库收集NAFLD相关靶点,利用Venny2.1获取药物与疾病交集靶点;使用Cytoscape3.9.1软件构建药物-活性成分-靶点网络;运用STRING数据库和Cytoscape3.9.1软件构建交集靶点蛋白相互作用(PPI)网络;利用DAVID数据库对交集靶点进行GO功能和KEGG通路富集分析;利用Discovery Studio 2019软件CDOCKER模块进行分子对接。结果共筛选得到110个活性成分及893个潜在作用靶点;获得核心活性成分2-十一酮、亚油酸乙酯、原海葱苷、黄芩素、山姜素,核心靶点AKT1、TNF、IL1B、IL6、PPARG;KEGG通路富集分析得到203条信号通路。分子对接结果表明,核心活性成分与核心靶点有较好结合活性。结论降脂1方可能通过2-十一酮、亚油酸乙酯、原海葱苷、黄芩素、山姜素等核心活性成分作用于AKT1、TNF-α、PPARG核心靶点,调控胰岛素抵抗相关通路及糖尿病并发症中的AGE-RAGE信号通路,从而发挥治疗NAFLD作用。展开更多
基金Project (50473013) supported by the National Natural Science Foundation of China
文摘Silicone rubber/polyacrylate sequential interpenetrating polymer networks(IPNs) were prepared by silicone rubber sheet dipped into the solution composed of different acrylate monomers and benzoyl peroxides(BPOs) for different time at room temperature and then acrylate polymerized at 80℃for 2 h. The molecular structure and damping properties of sequential IPNs were studied by means of FT-IR and dynamic mechanical analysis(DMA), respectively. The FT-IR spectrum shows that polyacrylate distributes unevenly along the thickness direction of IPNs, i.e. the concentration of polyacrylate decreases from the midst to the surface of the IPNs. The DMA shows that cold crystallization of silicone in the temperature range from -47℃to -30℃is reduced and loss factor of IPNs is improved after interpenetrating with polyacrylate. This suggestes that IPNs can be used as damping materials.
基金supported by the Scientific Research Foundation of Harbin Institute of Technology(HIT.2002.56)the Postdoctoral Foundation of Heilongjiang Province,China
文摘Interpenetrating polymer networks （IPNs） composed of acrylate-modified polyurethane （PU）/unsaturated polyester （UP） resin via simultaneous polymerization with various component ratios of PU/UP were prepared. The polymerization processes of IPNs were traced through infrared spectrum （IR） techniques, by which the phase separation in systems could be controlled effectively. Results for the morphology and miscibility among multiple phases of IPNs, obtained by transmission electron microscope （TEM） indicated that the domains between two phases were constricted in nanometer scales. The dynamic mechanical thermal analyzer （DMTA） detection results revealed that the loss factor （tanS） and loss modulus （E″） increased with the polyurethane amounts in system, and the peak value in curves of tanδ and E″ appeared toward low temperature ranges. Maximum tanδ values of all samples were above 0.3 in the nearly 50℃ ranges. Also, the mechanical properties of PU/UP IPNs were studied in detail.
文摘目的探讨轻盈祛浊汤治疗糖尿病肾病(DN)的作用机制。方法利用网络药理学预测轻盈祛浊汤治疗DN的作用机制,并通过分子对接预测活性成分的结合部位。结合网络药理学及分子对接结果,构建DN大鼠模型,2023年1—6月,将60只大鼠分为正常组、模型组、马来酸依那普利组、轻盈祛浊汤组,每组15只。治疗8周后比较四组大鼠24 h尿微量白蛋白(urinary microalbuminexcretion rate,UAER)、尿素氮、血肌酐水平和钠/葡萄糖协同转运蛋白1(recombinant sodium/glucose cotransporter 1,SGLT1),A1腺苷受体(A1 adenosine receptor,A1AR)蛋白表达水平。结果网络药理学共筛选出轻盈祛浊汤有效成分1114种,作用靶点269个,DN相关靶点2020个,其交集靶点174个。基因本体富集(GO)和基因组的京都百科全书(KEGG)分析得出主要涉及信号转导、炎症反应、细胞凋亡等一系列的生物学反应过程,主要参与丝裂原活化蛋白激酶/核因子κB(mitogen-activated protein kinase/nuclear factor kappa-B,pMAPK/NF-κB)、NOD样受体家族蛋白3/白细胞介素-1β(NOD-like receptor protein 3/interleukin-1β,NLRP3/IL-1β)、白细胞介素6/信号传导和转录激活因子3(interleukin-6/signal transducer and activator of transcription 3,IL-6/STAT3)、肿瘤坏死因子(tumor necrosis factor,TNF)、肿瘤蛋白p53(tumor protein 53,P53)和前列腺素内过氧化物合酶(prostaglandin-endoperoxide synthase 2,PTGS2)等信号通路的调控。分子对接表明,轻盈祛浊汤主要成分与DN靶点的结合活性较强。模型组大鼠24 h UAER[(4539.71±516.03)μg/24 h比(226.59±72.71)μg/24 h]、血肌酐[(85.63±12.96)mL·kg^(-1)·min^(-1)比(0.48±0.12)mL·kg^(-1)·min^(-1)]、SGLT1(1.17±0.07比0.82±0.06)高于正常组,而模型组大鼠尿素氮、A1AR低于正常组(P<0.05)。马来酸依那普利片组大鼠24 h UAER、血肌酐、SGLT1低于模型组,马来酸依那普利片组大鼠尿素氮、A1AR高于模型组(P<0.05)。轻盈祛浊汤组大鼠24 h UAER、血肌酐、SGLT1低于马来酸依那普利片组,而轻盈祛浊汤组大鼠尿素氮、A1AR高于马来酸依那普利片组(P<0.05)。结论轻盈祛浊汤可通过调节pMAPK/NF-κB、NLRP3/IL-1β、IL-6/STAT3、TNF、P53和PTGS2信号通路发挥治疗DN的作用。
文摘目的运用网络药理学和分子对接技术探究曾江涛经验方(降脂1方)治疗非酒精性脂肪性肝病(NAFLD)的作用机制。方法采用TCMSP和Herb数据库检索降脂1方活性成分及其作用靶点,通过GeneCards、OMIM及DisGeNET数据库收集NAFLD相关靶点,利用Venny2.1获取药物与疾病交集靶点;使用Cytoscape3.9.1软件构建药物-活性成分-靶点网络;运用STRING数据库和Cytoscape3.9.1软件构建交集靶点蛋白相互作用(PPI)网络;利用DAVID数据库对交集靶点进行GO功能和KEGG通路富集分析;利用Discovery Studio 2019软件CDOCKER模块进行分子对接。结果共筛选得到110个活性成分及893个潜在作用靶点;获得核心活性成分2-十一酮、亚油酸乙酯、原海葱苷、黄芩素、山姜素,核心靶点AKT1、TNF、IL1B、IL6、PPARG;KEGG通路富集分析得到203条信号通路。分子对接结果表明,核心活性成分与核心靶点有较好结合活性。结论降脂1方可能通过2-十一酮、亚油酸乙酯、原海葱苷、黄芩素、山姜素等核心活性成分作用于AKT1、TNF-α、PPARG核心靶点,调控胰岛素抵抗相关通路及糖尿病并发症中的AGE-RAGE信号通路,从而发挥治疗NAFLD作用。
