Urodynamic investigation in conscious rats is widely employed to explore functional bladder disorders of various etiologies and pathogeneses. Rats can be placed in restraining cages or wide cabinets where they are all...Urodynamic investigation in conscious rats is widely employed to explore functional bladder disorders of various etiologies and pathogeneses. Rats can be placed in restraining cages or wide cabinets where they are allowed to move freely during cystometry. However, the requirements of special devices hampered the application of urodynamic test in freely moving rats, and whether the restraint has any effects on urodynamic parameters in conscious rats remains obscure. In the present study, we described a novel approach for urodynamic investigation in both restrained and freely moving conscious rats. In addition, we for the first time systematically compared the urodynamic parameters of rats in the two conditions. With the current method, we successfully recorded stable and repeatable intravesical pressure traces and collected expected reliable data, which supported the idea that the restraint does not affect the activity of the micturition reflex in rats, provided sufficient and appropriate measures could be applied during cystometry. Fewer technique problems were encountered during urodynamic examination in restrained rats than in freely moving ones. Taken together, conscious cystometry in rats placed in restraining cages with proper managements is a reliable and practical approach for evaluating the detrusor activity and bladder function.展开更多
Objectives:Spinal cord injury(SCI)is one of the most debilitating and expensive traumatic conditions.Chronic complications after SCI have a particularly negative impact on patients’functional independence and quality...Objectives:Spinal cord injury(SCI)is one of the most debilitating and expensive traumatic conditions.Chronic complications after SCI have a particularly negative impact on patients’functional independence and quality of life.Urodynamic study(UDS)provides a quantitative assessment of lower urinary tract function in these patients.In many fields,animal models are considered a precursor to clinical trials,so research using laboratory animals play a major role in knowledge acquisition.Materials and methods:Twelve femaleWistar rats(13 weeks old,220-270 g)were divided randomly into 2 groups:sham or SCI.The sham-operated group underwent a laminectomy at T9-T10 without any spinal cord damage,while the SCI group underwent a complete transection at the T9-T10 vertebral level.We performed cystometry in all animals at the end of the fourth week.In this article,we visualize all procedures for catheter implementation and UDS in animals for the first time at Tabriz University of Medical Sciences,Iran,using a locally developed animal UDS device.Results:The UDS results showed that the bladders in the SCI group were overactive and that peak and baseline pressures increased significantly in rats with SCI when compared with the sham group(p<0.05 for all).Conversely,significant reductions in bladder compliance and intercontraction interval were observed in the SCI group(p<0.05 for both).Conclusions:This comprehensive visual report will be very useful to all researchers in the field of urology.Furthermore,the measurable variables of the UDS device have been described in this study.展开更多
文摘Urodynamic investigation in conscious rats is widely employed to explore functional bladder disorders of various etiologies and pathogeneses. Rats can be placed in restraining cages or wide cabinets where they are allowed to move freely during cystometry. However, the requirements of special devices hampered the application of urodynamic test in freely moving rats, and whether the restraint has any effects on urodynamic parameters in conscious rats remains obscure. In the present study, we described a novel approach for urodynamic investigation in both restrained and freely moving conscious rats. In addition, we for the first time systematically compared the urodynamic parameters of rats in the two conditions. With the current method, we successfully recorded stable and repeatable intravesical pressure traces and collected expected reliable data, which supported the idea that the restraint does not affect the activity of the micturition reflex in rats, provided sufficient and appropriate measures could be applied during cystometry. Fewer technique problems were encountered during urodynamic examination in restrained rats than in freely moving ones. Taken together, conscious cystometry in rats placed in restraining cages with proper managements is a reliable and practical approach for evaluating the detrusor activity and bladder function.
基金a financial support from the Student Research Committee of Tabriz University of Medical Sciences(grant no.63880).
文摘Objectives:Spinal cord injury(SCI)is one of the most debilitating and expensive traumatic conditions.Chronic complications after SCI have a particularly negative impact on patients’functional independence and quality of life.Urodynamic study(UDS)provides a quantitative assessment of lower urinary tract function in these patients.In many fields,animal models are considered a precursor to clinical trials,so research using laboratory animals play a major role in knowledge acquisition.Materials and methods:Twelve femaleWistar rats(13 weeks old,220-270 g)were divided randomly into 2 groups:sham or SCI.The sham-operated group underwent a laminectomy at T9-T10 without any spinal cord damage,while the SCI group underwent a complete transection at the T9-T10 vertebral level.We performed cystometry in all animals at the end of the fourth week.In this article,we visualize all procedures for catheter implementation and UDS in animals for the first time at Tabriz University of Medical Sciences,Iran,using a locally developed animal UDS device.Results:The UDS results showed that the bladders in the SCI group were overactive and that peak and baseline pressures increased significantly in rats with SCI when compared with the sham group(p<0.05 for all).Conversely,significant reductions in bladder compliance and intercontraction interval were observed in the SCI group(p<0.05 for both).Conclusions:This comprehensive visual report will be very useful to all researchers in the field of urology.Furthermore,the measurable variables of the UDS device have been described in this study.
