[Objectives]This study was conducted to establish a liquid chromatography-tandem mass spectrometry method for the determination of coumoxystrobin residue in milk.[Methods]A sample was ultrasonically extracted with ace...[Objectives]This study was conducted to establish a liquid chromatography-tandem mass spectrometry method for the determination of coumoxystrobin residue in milk.[Methods]A sample was ultrasonically extracted with acetonitrile,purified by a solid phase extraction column,concentrated by nitrogen blowing,and tested on a machine.[Results]In the range of 0.01-10.00 mg/kg,the linear relationship of coumoxystrobin was good.The linear equation was y=5045.96x+935.332,r^(2)=0.999.The detection limit was 0.002 mg/kg,and the quantitation limit was 0.006 mg/kg.The method has good stability,high precision,and the detection limit can meet the limit requirement of coumoxystrobin in GB 2763-2019.[Conclusions]This study can provide technical support for the supervision and monitoring of coumoxystrobin.展开更多
[Objectives]This study was conducted to establish a method for determining residual coumoxystrobin in vegetables using QeEChERS-liquid chromatography-tandem mass spectrometry(QeEChERS-LC-MS/MS).[Methods]The sample was...[Objectives]This study was conducted to establish a method for determining residual coumoxystrobin in vegetables using QeEChERS-liquid chromatography-tandem mass spectrometry(QeEChERS-LC-MS/MS).[Methods]The sample was extracted by acetonitrile,and the extract was purified by QeEChERS,concentrated by nitrogen blowing,and then detected.[Results]Coumoxystrobin had a good linear relation in the range of 0.01-10.0 mg/kg,and the linear equation was y=4686.92×x+5683.28,R^(2)=0.999.The limit of detection was 0.001 mg/kg,and the limit of quantitation was 0.003 mg/kg.[Conclusions]The method has the advantages of convenient and fast operation and stable detection process,and can provide technical support for the supervision and monitoring of coumoxystrobin.展开更多
[Objective] The paper was to screen suitable agents for controlling northern leaf blight of maize in field. [Method] Field efficacy test was conducted using the agents of propiconazole, azoxystrobin, propiconazole...[Objective] The paper was to screen suitable agents for controlling northern leaf blight of maize in field. [Method] Field efficacy test was conducted using the agents of propiconazole, azoxystrobin, propiconazole·azoxystrobin, coumoxystrobin·tebuconazole, coumoxystrobin·tebuconazole and azoxystrobin. [Result] At 14 d post secondary spraying, the control effects of 25% propionazole EC 168.8 g/hm^2, 25% azoxystrobin SC 300 g/hm^2, 18.7% propiconazole·azoxystrobin SC 196.4 g/hm^2, 40% coumoxystrobin·tebuconazole SC 180 m L/hm^2, 40% coumoxystrobin·tebuconazole SC 180 m L/hm^2＋ 25% azoxystrobin SC 300 g/hm^2 against northern leaf blight of maize were 77.35%, 77.11%, 78.13%, 74.60% and 80.94%, re-spectively. The agents were safe and harmless to maize. [Conclusion] Considering the production factors, 18.7% propiconazole·azoxystrobin SC196.4 g/hm^2 or 40% coumoxystrobin·tebuconazole SC 180 m L/hm^2＋ 25% azoxystrobin SC 300 g/hm^2 can be sprayed in production before the incidence or in the initial incidence of northern leaf blight of maize.展开更多
为筛选防治玉米大斑病适宜药剂,通过田间试验进行丙环·嘧菌酯等4种药剂田间药效试验。结果表明:25%丙环唑乳油168.8 g/hm^2、嘧菌酯悬浮剂300 g/hm^2、丙环·嘧菌酯悬浮剂196.4 g/hm^2、丁香·戊唑醇悬浮剂180 m L/hm^2、...为筛选防治玉米大斑病适宜药剂,通过田间试验进行丙环·嘧菌酯等4种药剂田间药效试验。结果表明:25%丙环唑乳油168.8 g/hm^2、嘧菌酯悬浮剂300 g/hm^2、丙环·嘧菌酯悬浮剂196.4 g/hm^2、丁香·戊唑醇悬浮剂180 m L/hm^2、丁香·戊唑醇悬浮剂180 m L/hm^2+嘧菌酯悬浮剂300 g/hm^2等5个处理2次施药后对玉米大斑病的防治效果分别为77.35%、77.11%、78.13%、74.60%和80.94%,对玉米安全、无药害;结合产量因素,在生产上防治大斑病宜采用丙环·嘧菌酯悬浮剂196.4 g/hm^2或丁香·戊唑醇悬浮剂180 m L/hm^2+嘧菌酯悬浮剂300 g/hm^2为佳,建议在发病前或发病初期及早用药防治。展开更多
基金Special Fund for Innovation Capacity Building of Scientific Research Institutions(QKHFQ[2019]4001)Science and Technology Program of Guizhou Province(QKHZC[2019]2451-8-3).
文摘[Objectives]This study was conducted to establish a liquid chromatography-tandem mass spectrometry method for the determination of coumoxystrobin residue in milk.[Methods]A sample was ultrasonically extracted with acetonitrile,purified by a solid phase extraction column,concentrated by nitrogen blowing,and tested on a machine.[Results]In the range of 0.01-10.00 mg/kg,the linear relationship of coumoxystrobin was good.The linear equation was y=5045.96x+935.332,r^(2)=0.999.The detection limit was 0.002 mg/kg,and the quantitation limit was 0.006 mg/kg.The method has good stability,high precision,and the detection limit can meet the limit requirement of coumoxystrobin in GB 2763-2019.[Conclusions]This study can provide technical support for the supervision and monitoring of coumoxystrobin.
基金Supported by Natural Science Foundation of Hunan Province(2024JJ8259).
文摘[Objectives]This study was conducted to establish a method for determining residual coumoxystrobin in vegetables using QeEChERS-liquid chromatography-tandem mass spectrometry(QeEChERS-LC-MS/MS).[Methods]The sample was extracted by acetonitrile,and the extract was purified by QeEChERS,concentrated by nitrogen blowing,and then detected.[Results]Coumoxystrobin had a good linear relation in the range of 0.01-10.0 mg/kg,and the linear equation was y=4686.92×x+5683.28,R^(2)=0.999.The limit of detection was 0.001 mg/kg,and the limit of quantitation was 0.003 mg/kg.[Conclusions]The method has the advantages of convenient and fast operation and stable detection process,and can provide technical support for the supervision and monitoring of coumoxystrobin.
基金Supported by National Key Research and Development Program"Scientific and Technological Innovation in Food Production"(2017YFD0300606)Science and Technology Development Project of Jilin Province(20170412029XH)
文摘[Objective] The paper was to screen suitable agents for controlling northern leaf blight of maize in field. [Method] Field efficacy test was conducted using the agents of propiconazole, azoxystrobin, propiconazole·azoxystrobin, coumoxystrobin·tebuconazole, coumoxystrobin·tebuconazole and azoxystrobin. [Result] At 14 d post secondary spraying, the control effects of 25% propionazole EC 168.8 g/hm^2, 25% azoxystrobin SC 300 g/hm^2, 18.7% propiconazole·azoxystrobin SC 196.4 g/hm^2, 40% coumoxystrobin·tebuconazole SC 180 m L/hm^2, 40% coumoxystrobin·tebuconazole SC 180 m L/hm^2＋ 25% azoxystrobin SC 300 g/hm^2 against northern leaf blight of maize were 77.35%, 77.11%, 78.13%, 74.60% and 80.94%, re-spectively. The agents were safe and harmless to maize. [Conclusion] Considering the production factors, 18.7% propiconazole·azoxystrobin SC196.4 g/hm^2 or 40% coumoxystrobin·tebuconazole SC 180 m L/hm^2＋ 25% azoxystrobin SC 300 g/hm^2 can be sprayed in production before the incidence or in the initial incidence of northern leaf blight of maize.
文摘为筛选防治玉米大斑病适宜药剂,通过田间试验进行丙环·嘧菌酯等4种药剂田间药效试验。结果表明:25%丙环唑乳油168.8 g/hm^2、嘧菌酯悬浮剂300 g/hm^2、丙环·嘧菌酯悬浮剂196.4 g/hm^2、丁香·戊唑醇悬浮剂180 m L/hm^2、丁香·戊唑醇悬浮剂180 m L/hm^2+嘧菌酯悬浮剂300 g/hm^2等5个处理2次施药后对玉米大斑病的防治效果分别为77.35%、77.11%、78.13%、74.60%和80.94%,对玉米安全、无药害;结合产量因素,在生产上防治大斑病宜采用丙环·嘧菌酯悬浮剂196.4 g/hm^2或丁香·戊唑醇悬浮剂180 m L/hm^2+嘧菌酯悬浮剂300 g/hm^2为佳,建议在发病前或发病初期及早用药防治。
