Tracking the movement of droplets in digital microfluidics is essential to improve its control stability and obtain dynamic information for its applications such as point-of-care testing,environment monitoring and che...Tracking the movement of droplets in digital microfluidics is essential to improve its control stability and obtain dynamic information for its applications such as point-of-care testing,environment monitoring and chemical synthesis.Herein,an intelligent,accurate and fast droplet tracking method based on machine vision is developed for applications of digital microfluidics.To continuously recognize the transparent droplets in real-time and avoid the interferes from background patterns or inhomogeneous illumination,we introduced the correlation filter tracker,enabling online learning of the multi-features of the droplets in Fourier domain.Results show the proposed droplet tracking method could accurately locate the droplets.We also demonstrated the capacity of the proposed method for estimation of the droplet velocity as faster as 20 mm/s,and its application in online monitoring the Griess reaction for both colorimetric assay of nitrite and study of reaction kinetics.展开更多
基金the financial support from the National Natural Science Foundation of China(Nos.31701698,81972017)Shanghai Key Laboratory of Forensic Medicine,Academy of Forensic Science(No.KF1910)Shanghai Shenkang Hospital Development Center to promote clinical skills and clinical innovation ability in municipal hospitals of the Three-year Action Plan Project(No.SHDC2020CR3006A).
文摘Tracking the movement of droplets in digital microfluidics is essential to improve its control stability and obtain dynamic information for its applications such as point-of-care testing,environment monitoring and chemical synthesis.Herein,an intelligent,accurate and fast droplet tracking method based on machine vision is developed for applications of digital microfluidics.To continuously recognize the transparent droplets in real-time and avoid the interferes from background patterns or inhomogeneous illumination,we introduced the correlation filter tracker,enabling online learning of the multi-features of the droplets in Fourier domain.Results show the proposed droplet tracking method could accurately locate the droplets.We also demonstrated the capacity of the proposed method for estimation of the droplet velocity as faster as 20 mm/s,and its application in online monitoring the Griess reaction for both colorimetric assay of nitrite and study of reaction kinetics.
