The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base edit...The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.展开更多
Since 2013,China has been the world’s largest market for industrial robots.Despite the gradual maturity of the industrial robot system,the lagging R&D and backward technology level of industrial robots have led t...Since 2013,China has been the world’s largest market for industrial robots.Despite the gradual maturity of the industrial robot system,the lagging R&D and backward technology level of industrial robots have led to a strong dependence on the import of core components and key technologies,which to a certain extent has restricted the development and improvement of industrial robots.At present,the“neck problem”in the field of industrial robots in China is not only in the reducer,controller,and servo but also in the basic processing equipment,basic technology,and basic materials.In this paper,we propose measures to improve the“neck problem”of industrial robots to promote the high-quality development of industrial robots in China.展开更多
Three kinds of landing gear actuating cylinder inner-locks of an aircrafi ： block ring lock, steel ball lock, finger lock are taken as the study object based on the mechanics, geometry, materials, technology etc and ...Three kinds of landing gear actuating cylinder inner-locks of an aircrafi ： block ring lock, steel ball lock, finger lock are taken as the study object based on the mechanics, geometry, materials, technology etc and some aircrafi typical inner-lock practical applications. The working principle of the three typical actuating cylinder innerlocks are expounded and the stress and workmanship requirements of the three inner-lock core components are analyzed. The advantages and disadvantages of different kinds are compared and the characteristics and applications of the three inner-locks investigated. The research and analysis results provide valuable information for the actuating cylinder inner-lock of the aircraft landing gear design.展开更多
The chemical modifications of DNA and proteins are powerful mechanisms for regulating molecular and biological functions,influencing a wide array of signaling pathways in eukaryotes.Recent advance-ments in epitranscri...The chemical modifications of DNA and proteins are powerful mechanisms for regulating molecular and biological functions,influencing a wide array of signaling pathways in eukaryotes.Recent advance-ments in epitranscriptomics have shown that RNA modifications play crucial roles in diverse biological processes.Since their discovery in the 1970s,scientists have sought to decipher,identify,and elucidate the functions of these modifications across biological systems.Over the past decade,mounting evi-dence has demonstrated the importance of RNA modification pathways in plants,prompting significant efforts to decipher their physiological relevance.With the advent of high-resolution mapping tech-niques for RNA modifications and the gradual uncovering of their biological roles,our understanding of this additional layer of regulation is beginning to take shape.In this review,we summarize recent findings on the major RNA modifications identified in plants,with an emphasis on N^(6)-methyladenosine(m^(6)A),the most extensively studied modification.We discuss the functional signifi-cance of the effector components involved in m^(6)A modification and its diverse roles in plant biotic in-teractions,including plant–virus,plant–bacterium,plant–fungus,and plant–insect relationships.Furthermore,we highlight new technological developments driving research progress in this field and outline key challenges that remain to be addressed.展开更多
Pulse tube cryocoolers working at liquid helium temperatures(LHe-PTCs),particularly those in a separated configuration-where the rotary valve assembly is positioned remotely from the cold head-exhibit high reliability...Pulse tube cryocoolers working at liquid helium temperatures(LHe-PTCs),particularly those in a separated configuration-where the rotary valve assembly is positioned remotely from the cold head-exhibit high reliability,long service life,low vibration,and strong resistance to electromagnetic interference.These characteristics make them indispensable for cryogen-free systems.Separated LHe-PTCs are widely used in advanced applications such as quantum physics[1]and particle physics[2],serving as core components in instruments including dilution refrigerators,scanning tunneling microscopes(STM),and superconducting magnet systems.展开更多
Accelerate advancing the largest offshore wind power monopile export project undertaken in China;Provide core components for the world's largest floating offshore wind farm project with single rapacity;Build the l...Accelerate advancing the largest offshore wind power monopile export project undertaken in China;Provide core components for the world's largest floating offshore wind farm project with single rapacity;Build the large-scale manufacturing capacity of 100-meter-level large-scale blades,providing strong support for meeting the demand for wind power equipment in the Bohai Rim region.....Recently,many enterprises of CSSC have frequently reported good news in the offshore wind power equipment sector.展开更多
基金supported by the Beijing Scholars Program[BSP041]。
文摘The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.
文摘Since 2013,China has been the world’s largest market for industrial robots.Despite the gradual maturity of the industrial robot system,the lagging R&D and backward technology level of industrial robots have led to a strong dependence on the import of core components and key technologies,which to a certain extent has restricted the development and improvement of industrial robots.At present,the“neck problem”in the field of industrial robots in China is not only in the reducer,controller,and servo but also in the basic processing equipment,basic technology,and basic materials.In this paper,we propose measures to improve the“neck problem”of industrial robots to promote the high-quality development of industrial robots in China.
文摘Three kinds of landing gear actuating cylinder inner-locks of an aircrafi ： block ring lock, steel ball lock, finger lock are taken as the study object based on the mechanics, geometry, materials, technology etc and some aircrafi typical inner-lock practical applications. The working principle of the three typical actuating cylinder innerlocks are expounded and the stress and workmanship requirements of the three inner-lock core components are analyzed. The advantages and disadvantages of different kinds are compared and the characteristics and applications of the three inner-locks investigated. The research and analysis results provide valuable information for the actuating cylinder inner-lock of the aircraft landing gear design.
基金funded by the National Natural Science Foundation of China(32320103010)the National Key Research and Development Program of China(2021YFD1400400)to F.L.+1 种基金the National Natural Science Foundation of China(32302318)to L.G.the National Natural Science Foundation of China(32001868)to H.S.
文摘The chemical modifications of DNA and proteins are powerful mechanisms for regulating molecular and biological functions,influencing a wide array of signaling pathways in eukaryotes.Recent advance-ments in epitranscriptomics have shown that RNA modifications play crucial roles in diverse biological processes.Since their discovery in the 1970s,scientists have sought to decipher,identify,and elucidate the functions of these modifications across biological systems.Over the past decade,mounting evi-dence has demonstrated the importance of RNA modification pathways in plants,prompting significant efforts to decipher their physiological relevance.With the advent of high-resolution mapping tech-niques for RNA modifications and the gradual uncovering of their biological roles,our understanding of this additional layer of regulation is beginning to take shape.In this review,we summarize recent findings on the major RNA modifications identified in plants,with an emphasis on N^(6)-methyladenosine(m^(6)A),the most extensively studied modification.We discuss the functional signifi-cance of the effector components involved in m^(6)A modification and its diverse roles in plant biotic in-teractions,including plant–virus,plant–bacterium,plant–fungus,and plant–insect relationships.Furthermore,we highlight new technological developments driving research progress in this field and outline key challenges that remain to be addressed.
基金supported by the National Natural Science Foun-dation of China(52476020)the National Key Research and Devel-opment Program of China(2023YFF0721304)the“Pioneer”and“Leading Goose”Research and Development Program of Zhe-jiang Province,China(2025C01080).
文摘Pulse tube cryocoolers working at liquid helium temperatures(LHe-PTCs),particularly those in a separated configuration-where the rotary valve assembly is positioned remotely from the cold head-exhibit high reliability,long service life,low vibration,and strong resistance to electromagnetic interference.These characteristics make them indispensable for cryogen-free systems.Separated LHe-PTCs are widely used in advanced applications such as quantum physics[1]and particle physics[2],serving as core components in instruments including dilution refrigerators,scanning tunneling microscopes(STM),and superconducting magnet systems.
文摘Accelerate advancing the largest offshore wind power monopile export project undertaken in China;Provide core components for the world's largest floating offshore wind farm project with single rapacity;Build the large-scale manufacturing capacity of 100-meter-level large-scale blades,providing strong support for meeting the demand for wind power equipment in the Bohai Rim region.....Recently,many enterprises of CSSC have frequently reported good news in the offshore wind power equipment sector.
