To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuc...To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuclear transfer （SCNT） by controlled freezing and vitrification.This experiment,therefore,was designed to compare the cryopreservation of these blastocysts with controlled freezing and OPS vitrification.Adenosine-5’-triphosphate （ATP） content and reactive oxygen species （ROS） level in blastocysts were also analyzed.Firstly,for each type of blastocyst （IVF,ICSI or SCNT）,significant differences were observed between the survival rates of the controlled freezing （（81.56±2.33）,（68.18±4.72） or （47.89±5.83）%） and OPS vitrification groups （（92.24±4.54）,（82.40±3.76） or （78.71±5.91）%;P〈0.05）.Secondly,for each type of blastocyst （IVF,ICSI or SCNT）,ATP content was significantly decreased after controlled freezing or vitrification,and the ATP content in the controlled freezing group （0.43±0.06）,（0.35±0.05） or （0.21±0.02） pmol） was significantly lower than that found in the OPS vitrification group （0.62±0.04）,（0.46±0.03） or （0.30±0.01） pmol;P〈0.05）.Thirdly,ROS level in fresh IVF （（47.33±3.56） c.p.s （counted photons per second）,ICSI （（36.51±2.58） c.p.s） or SCNT blastocysts （（26.44±1.49） c.p.s） was significantly lower than that found in the OPS vitrification group （（72.14±4.31）,（58.89±3.89） or （40.11±5.73） c.p.s;P〈0.05）,but higher than that of the controlled freezing group （34.41±3.32）,（23.13±1.26） or （15.46±2.45） c.p.s;P〈0.05）.The present study indicated that vitrification is more efficient in the cryopreservation of bovine blastocysts derived from IVF,ICSI or SCNT than controlled freezing.Furthermore,both vitrification and controlled freezing significantly altered the ATP content and ROS level in those blastocysts.展开更多
Sugar beet（Beta vulgaris L.） is an industrial crop, grown worldwide for sugar production. In Pakistan, sugar is mostly extracted from sugarcane, soil and environmental conditions are equally favorable for sugar beet...Sugar beet（Beta vulgaris L.） is an industrial crop, grown worldwide for sugar production. In Pakistan, sugar is mostly extracted from sugarcane, soil and environmental conditions are equally favorable for sugar beet cultivation. Beet sugar contents are higher than sugarcane sugar contents, which can be further increased by potassium（K） fertilization. Total K concentration is higher in Pakistani soils developed from mica minerals, but it does not represent plant available K for sustainable plant growth. A pot experiment was conducted in the wire-house of Institute of Soil and Environmental Sciences at University of Agriculture Faisalabad, Pakistan. K treatments were the following： no K（K_0）, K application at 148 kg ha^（–1）（K_1） and 296 kg ha^（–1）（K_2）. Irrigation levels were used as water sufficient at 60% water holding capacity and water deficient at 40% water holding capacity. The growth, yield and beet quality data were analyzed statistically using LSD. The results revealed that increase in the level of K fertilization at water sufficient level significantly increased plant growth, beet yield and industrial beet sugar content. The response of K fertilization under water deficient condition was also similar, however overall sugar production was less than that in water sufficient conditions. It is concluded from this study that K application could be used not only to enhance plant growth and beet yield but also enhance beet sugar content both under water-deficient as well as water-sufficient conditions.展开更多
This study aimed to examine the surface and content validity of the Mentoring Function Scale for Novice Nurses, used to assess the mentoring of entry-level nurses, and to refine the scale items. In Study 1, six nurse ...This study aimed to examine the surface and content validity of the Mentoring Function Scale for Novice Nurses, used to assess the mentoring of entry-level nurses, and to refine the scale items. In Study 1, six nurse education researchers, selected using convenience sampling, with five or more years of nursing experience and experience teaching novice nurses, were invited to an expert meeting in July 2015. A group interview was conducted that lasted approximately 120 minutes. Study 2 examined the content validity index. Between September and November 2015, we distributed a self-administered questionnaire survey to 11 participants selected by convenience sampling. The participants included five nurse education researchers with a minimum of five years of nursing experience and experience teaching novice nurses, as well as six clinical nurses with a master’s degree or higher. Finally, 81 questionnaire items were retained from the initial 125 items. The 81-item Mentoring Function Scale for Novice Nurses had higher content validity than the original scale. To further increase the scale’s applicability, future studies should assess its reliability, construct validity, and criterion-related validity.展开更多
We have investigated a correlation of transcript abundances of key genes that influence the quality of wood and flavonoid biosynthesis, such as the two p-hydroxycinnamoyl-CoA:quinate shikimate p-hydroxycinnamoyl trans...We have investigated a correlation of transcript abundances of key genes that influence the quality of wood and flavonoid biosynthesis, such as the two p-hydroxycinnamoyl-CoA:quinate shikimate p-hydroxycinnamoyl transferase (HCT) and the two chalcone synthases (CHS) from Eucalyptus globulus grown in a greenhouse. The EglHCT1 and EglHCT2 transcripts accumulated in stems of all ages, but to a lesser extent in leaves. On the other hand, EglCHS3 and EglCHS4 exhibited high transcript levels in leaves, roots and shoots, but low levels in the stem. A positive correlation (R2 > 0.70) was observed between the transcript levels of the EglHCT1, EglHCT2 genes and Klason lignin (KL) content. In addition, the sum of transcript levels of EglHCT1 and EglHCT2 genes were highly correlated to KL contents (R2 > 0.85). However, there is no relationship between transcript levels of two CHS genes and, KL or flavonoid contents. This may imply that lignin biosynthesis and flavonoid biosynthesis are independently regulated in E. globulus.展开更多
Coastal hazards induced by meteo-marine forcing are exacerbated by sea level change along the West African coastline. Changes in sea level are induced by ocean processes such as ocean heat content and river discharge....Coastal hazards induced by meteo-marine forcing are exacerbated by sea level change along the West African coastline. Changes in sea level are induced by ocean processes such as ocean heat content and river discharge. However, although these processes control largely change in sea level, they remain poorly understood. This study analyzes changes in ocean heat content, river discharge, and sea level and establishes an interconnection between these parameters using several statistical methods over the 1993-2021 period. Results showed a significant correlation between sea level and ocean heat content at 2000 m depth. The yearly minimum value appears in July from Cote d’Ivoire to Benin, whilst this value appears in June in Nigeria. The temporal variability of ocean heat content, river discharge and sea level along the West African coastline exhibits three or four periods interrupted by some breakpoints with unequal duration. The results indicate that the 1993-2000 period was dominated by an increasing ocean heat content along the coastline, while the period after the 2000s exhibits mostly a decreasing trend. Positive and negative trends characterized river discharge and sea level along this coastline. The result of multiple linear regression between sea level, river discharge and ocean heat content is a good approximation of sea level trend along the West African coastline. The results of this study could be used to predict future sea level trends along the coast.展开更多
The single-phase three-level voltage source inverter based on wavelet modulation(WM) is proposed.The WM technique is based on constructing a nondyadic-type multi-resolution analysis(MRA),which supports sampling contin...The single-phase three-level voltage source inverter based on wavelet modulation(WM) is proposed.The WM technique is based on constructing a nondyadic-type multi-resolution analysis(MRA),which supports sampling continuous-time sinusoidal signals in a nonuniform recurrent manner,and then reconstructing it by using inverter switching actions. In order to further improve the output voltage waveform and reduce harmonic distortion,the wavelet modulation is used to three-level inverter. The high magnitude of fundamental component and significantly reduced harmonic contents of the inverter output voltage can be achieved by using WM in the single-phase three-level voltage source inverter. Furthermore,the WM algorithm is implemented by using only one element government(EV) in DSP. The simulated and experimental results prove the accuracy and feasibility of the WM scheme for single-phase three-level voltage source inverter.展开更多
In this paper, we proposed a metric to measure the shift invariance of the three different contourlet transforms. And then, using the same structure texture image retrieval system which use subband coefficients energy...In this paper, we proposed a metric to measure the shift invariance of the three different contourlet transforms. And then, using the same structure texture image retrieval system which use subband coefficients energy, standard deviation and kurtosis features with Canberra distance, we gave a comparison of their texture description abilities. Experimental results show that contourlet-2.3 texture image retrieval system has almost retrieval rates with non-sub sampled contourlet system;the two systems have better retrieval results than the original contourlet retrieval system. On the other hand, for the relatively lower redundancy, we recommend using contourlet- 2.3 as texture description transform.展开更多
基金supported by the Fund of China Agriculture Research System(CARS-37)the Genetically Modified Organisms Breeding Major Projects of China(2009ZX08011-031B)+1 种基金the Basic Research Fund of IAS,CAAS(2010jc-3-1)the National Natural Science Foundation of China(31001011)
文摘To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuclear transfer （SCNT） by controlled freezing and vitrification.This experiment,therefore,was designed to compare the cryopreservation of these blastocysts with controlled freezing and OPS vitrification.Adenosine-5’-triphosphate （ATP） content and reactive oxygen species （ROS） level in blastocysts were also analyzed.Firstly,for each type of blastocyst （IVF,ICSI or SCNT）,significant differences were observed between the survival rates of the controlled freezing （（81.56±2.33）,（68.18±4.72） or （47.89±5.83）%） and OPS vitrification groups （（92.24±4.54）,（82.40±3.76） or （78.71±5.91）%;P〈0.05）.Secondly,for each type of blastocyst （IVF,ICSI or SCNT）,ATP content was significantly decreased after controlled freezing or vitrification,and the ATP content in the controlled freezing group （0.43±0.06）,（0.35±0.05） or （0.21±0.02） pmol） was significantly lower than that found in the OPS vitrification group （0.62±0.04）,（0.46±0.03） or （0.30±0.01） pmol;P〈0.05）.Thirdly,ROS level in fresh IVF （（47.33±3.56） c.p.s （counted photons per second）,ICSI （（36.51±2.58） c.p.s） or SCNT blastocysts （（26.44±1.49） c.p.s） was significantly lower than that found in the OPS vitrification group （（72.14±4.31）,（58.89±3.89） or （40.11±5.73） c.p.s;P〈0.05）,but higher than that of the controlled freezing group （34.41±3.32）,（23.13±1.26） or （15.46±2.45） c.p.s;P〈0.05）.The present study indicated that vitrification is more efficient in the cryopreservation of bovine blastocysts derived from IVF,ICSI or SCNT than controlled freezing.Furthermore,both vitrification and controlled freezing significantly altered the ATP content and ROS level in those blastocysts.
文摘Sugar beet（Beta vulgaris L.） is an industrial crop, grown worldwide for sugar production. In Pakistan, sugar is mostly extracted from sugarcane, soil and environmental conditions are equally favorable for sugar beet cultivation. Beet sugar contents are higher than sugarcane sugar contents, which can be further increased by potassium（K） fertilization. Total K concentration is higher in Pakistani soils developed from mica minerals, but it does not represent plant available K for sustainable plant growth. A pot experiment was conducted in the wire-house of Institute of Soil and Environmental Sciences at University of Agriculture Faisalabad, Pakistan. K treatments were the following： no K（K_0）, K application at 148 kg ha^（–1）（K_1） and 296 kg ha^（–1）（K_2）. Irrigation levels were used as water sufficient at 60% water holding capacity and water deficient at 40% water holding capacity. The growth, yield and beet quality data were analyzed statistically using LSD. The results revealed that increase in the level of K fertilization at water sufficient level significantly increased plant growth, beet yield and industrial beet sugar content. The response of K fertilization under water deficient condition was also similar, however overall sugar production was less than that in water sufficient conditions. It is concluded from this study that K application could be used not only to enhance plant growth and beet yield but also enhance beet sugar content both under water-deficient as well as water-sufficient conditions.
文摘This study aimed to examine the surface and content validity of the Mentoring Function Scale for Novice Nurses, used to assess the mentoring of entry-level nurses, and to refine the scale items. In Study 1, six nurse education researchers, selected using convenience sampling, with five or more years of nursing experience and experience teaching novice nurses, were invited to an expert meeting in July 2015. A group interview was conducted that lasted approximately 120 minutes. Study 2 examined the content validity index. Between September and November 2015, we distributed a self-administered questionnaire survey to 11 participants selected by convenience sampling. The participants included five nurse education researchers with a minimum of five years of nursing experience and experience teaching novice nurses, as well as six clinical nurses with a master’s degree or higher. Finally, 81 questionnaire items were retained from the initial 125 items. The 81-item Mentoring Function Scale for Novice Nurses had higher content validity than the original scale. To further increase the scale’s applicability, future studies should assess its reliability, construct validity, and criterion-related validity.
文摘We have investigated a correlation of transcript abundances of key genes that influence the quality of wood and flavonoid biosynthesis, such as the two p-hydroxycinnamoyl-CoA:quinate shikimate p-hydroxycinnamoyl transferase (HCT) and the two chalcone synthases (CHS) from Eucalyptus globulus grown in a greenhouse. The EglHCT1 and EglHCT2 transcripts accumulated in stems of all ages, but to a lesser extent in leaves. On the other hand, EglCHS3 and EglCHS4 exhibited high transcript levels in leaves, roots and shoots, but low levels in the stem. A positive correlation (R2 > 0.70) was observed between the transcript levels of the EglHCT1, EglHCT2 genes and Klason lignin (KL) content. In addition, the sum of transcript levels of EglHCT1 and EglHCT2 genes were highly correlated to KL contents (R2 > 0.85). However, there is no relationship between transcript levels of two CHS genes and, KL or flavonoid contents. This may imply that lignin biosynthesis and flavonoid biosynthesis are independently regulated in E. globulus.
文摘Coastal hazards induced by meteo-marine forcing are exacerbated by sea level change along the West African coastline. Changes in sea level are induced by ocean processes such as ocean heat content and river discharge. However, although these processes control largely change in sea level, they remain poorly understood. This study analyzes changes in ocean heat content, river discharge, and sea level and establishes an interconnection between these parameters using several statistical methods over the 1993-2021 period. Results showed a significant correlation between sea level and ocean heat content at 2000 m depth. The yearly minimum value appears in July from Cote d’Ivoire to Benin, whilst this value appears in June in Nigeria. The temporal variability of ocean heat content, river discharge and sea level along the West African coastline exhibits three or four periods interrupted by some breakpoints with unequal duration. The results indicate that the 1993-2000 period was dominated by an increasing ocean heat content along the coastline, while the period after the 2000s exhibits mostly a decreasing trend. Positive and negative trends characterized river discharge and sea level along this coastline. The result of multiple linear regression between sea level, river discharge and ocean heat content is a good approximation of sea level trend along the West African coastline. The results of this study could be used to predict future sea level trends along the coast.
基金Sponsored by the National Natural Science Foundation of China(Grant No.51107016)
文摘The single-phase three-level voltage source inverter based on wavelet modulation(WM) is proposed.The WM technique is based on constructing a nondyadic-type multi-resolution analysis(MRA),which supports sampling continuous-time sinusoidal signals in a nonuniform recurrent manner,and then reconstructing it by using inverter switching actions. In order to further improve the output voltage waveform and reduce harmonic distortion,the wavelet modulation is used to three-level inverter. The high magnitude of fundamental component and significantly reduced harmonic contents of the inverter output voltage can be achieved by using WM in the single-phase three-level voltage source inverter. Furthermore,the WM algorithm is implemented by using only one element government(EV) in DSP. The simulated and experimental results prove the accuracy and feasibility of the WM scheme for single-phase three-level voltage source inverter.
文摘In this paper, we proposed a metric to measure the shift invariance of the three different contourlet transforms. And then, using the same structure texture image retrieval system which use subband coefficients energy, standard deviation and kurtosis features with Canberra distance, we gave a comparison of their texture description abilities. Experimental results show that contourlet-2.3 texture image retrieval system has almost retrieval rates with non-sub sampled contourlet system;the two systems have better retrieval results than the original contourlet retrieval system. On the other hand, for the relatively lower redundancy, we recommend using contourlet- 2.3 as texture description transform.
