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Analytical solution of axisymmetric contact problem about indentation of a circular indenter into a soft functionally graded elastic layer 被引量:2
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作者 Sergey Volkov Sergey Aizikovich +1 位作者 Yue-Sheng Wang Igor Fedotov 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2013年第2期196-201,共6页
The paper addresses a contact problem of the theory of elasticity,i.e.,the penetration of a circular indenter with a flat base into a soft functionally graded elastic layer.The elastic properties of a functionally gra... The paper addresses a contact problem of the theory of elasticity,i.e.,the penetration of a circular indenter with a flat base into a soft functionally graded elastic layer.The elastic properties of a functionally graded layer arbitrarily vary with depth,and the foundation is assumed to be elastic,yet much harder than a layer.Approximated analytical solution is constructed,and it is shown that the solutions are asymptotically exact both for large and small values of characteristic dimensionless geometrical parameter of the problem.Numerical examples are analyzed for the cases of monotonic and nonmonotonic variations of elastic properties.Numerical results for the case of homogeneous layer are compared with the results for nondeformable foundation. 展开更多
关键词 contact problems·Indentation·functionally graded layer
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A Global Minimization Algorithm for Empirical Contact Potential Functions
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作者 WANG Yu-hong LI Wei 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2006年第2期248-250,共3页
Global minimization algorithm is indispensable for solving protein folding problems based on thermodynamic hypothesis. A contact difference (CD) based on pseudo potential function, for simulating empirical contact p... Global minimization algorithm is indispensable for solving protein folding problems based on thermodynamic hypothesis. A contact difference (CD) based on pseudo potential function, for simulating empirical contact potential functions and testing global minimization algorithm was proposed. The present article describes a conformational sampiing and global minimization algorithm, which is called WL, based on Monte Carlo simulation and simulated annealing. It can be used to locate CD's globe minimum and refold extended protein structures, as small as 0. 03 nm, from the native structures, back to ones with root mean square distance(RMSD). These results demonstrate that the global minimization problems for empirical contact potential functions may be solvable. 展开更多
关键词 Global minimization contact potential function Simulated annealing Monte Carlo simulation
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A Global Minimization Algorithm for Empirical Contact Potential Functions
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作者 WANGYu-hong LIWei 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2004年第5期572-574,共3页
Global minimization algorithm is indispensable to solving the protein folding problem based upon thermodynamic hypothesis. Here we propose a pseudo potential function, contact difference(CD), for simulating empirical ... Global minimization algorithm is indispensable to solving the protein folding problem based upon thermodynamic hypothesis. Here we propose a pseudo potential function, contact difference(CD), for simulating empirical contact potential functions and testing global minimization algorithm. The present paper covers conformational sampling and global minimization algorithm called BML03, based upon Monte Carlo and simulated annealing, which is able to locate CD′s global minimum and refold extended protein structures into ones with root mean square distance(RMSD) as small as 0.03 nm from the native structures. For empirical contact potential functions, these results demonstrate that their global minimization problems may be solvable. 展开更多
关键词 Global minimization contact potential function Simulated annealing Monte Carlo simulation
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The axisymmetric torsional contact problem of a functionally graded piezoelectric coated half-space 被引量:2
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作者 Jie Su Liao-Liang Ke +1 位作者 Yue-Sheng Wang Yang Xiang 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2017年第2期406-414,共9页
In this article, we study the axisymmetric torsional contact problem of a half-space coated with functionally graded piezoelectric material (FGPM) and subjected to a rigid circular punch. It is found that, along the t... In this article, we study the axisymmetric torsional contact problem of a half-space coated with functionally graded piezoelectric material (FGPM) and subjected to a rigid circular punch. It is found that, along the thickness direction, the electromechanical properties of FGPMs change exponentially. We apply the Hankel integral transform technique and reduce the problem to a singular integral equation, and then numerically determine the unknown contact stress and electric displacement at the contact surface. The results show that the surface contact stress, surface azimuthal displacement, surface electric displacement, and inner electromechanical field are obviously dependent on the gradient index of the FGPM coating. It is found that we can adjust the gradient index of the FGPM coating to modify the distributions of the electric displacement and contact stress. 展开更多
关键词 Axisymmetric torsional contact Singular integral equation functionally graded piezoelectric materials (FGPMs)
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THE DISTRIBUTION FUNCTION OF RESIDUE-RESIDUE CONTACTS IN PROTEIN MOLECULES
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作者 王向红 《Chinese Journal of Polymer Science》 SCIE CAS CSCD 2005年第4期387-392,共6页
In protein molecules each residue has a different ability to form contacts.In this paper,we calculated the number of contacts per residue and investigated the distribution of residue-residue contacts from 495 globular... In protein molecules each residue has a different ability to form contacts.In this paper,we calculated the number of contacts per residue and investigated the distribution of residue-residue contacts from 495 globular protein molecules using Contacts of Structural Units(CSU)software.It was found that the probability P(n)of amino acid residues having n pairs of contacts in all contacts fits Gaussian distribution very well.The distribution function of residue-residue contacts can be expressed as:P(n)=P_0+aexp[-b(n-n_c)~2].In our calculation,P_0=-0.06,α=11.4,b=-0.04 and n_c=9.0.According to distribution function,we found that those hydrophobic(H)residues including Leu,Val,Ile,Met,Phe,Tyr,Cys,and Trp residues have large values of the most probable number of contact n_c,and hydrophilic(P)residues including Ala,Gly,Thr, His,Glu,Gln,Asp,Asn,Lys,Ser,Arg,and Pro residues have the small ones.We also compare with Fauchere-Pliska hydrophobicity scale(FPH)and the most probable number of contact n_c for 20 amino acid residues,and find that there exists a linear relationship between Fauchere-Pliska hydrophobicity scale(FPH)and the most probable number of contact n_c, and it is expressed as:n_c=a+b×FPH,here α=8.87,and b=1.15.It is important to further explain protein folding and its stability from residue-residue contacts. 展开更多
关键词 Amino acid contact Distribution function Hydrophobic and hydrophilic residue Fauchere-Pliska hydrophobicity scale
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Solving frictional contact problems by two aggregate-function-based algorithms
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作者 Suyan He Hongwu Zhang Xingsi Li 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2005年第5期467-471,共5页
Three dimensional frictional contact problems are formulated as linear complementarity problems based on the parametric variational principle. Two aggregate-functionbased algorithms for solving complementarity problem... Three dimensional frictional contact problems are formulated as linear complementarity problems based on the parametric variational principle. Two aggregate-functionbased algorithms for solving complementarity problems are proposed. One is called the self-adjusting interior point algorithm, the other is called the aggregate function smoothing algorithm. Numerical experiment shows the efficiency of the proposed two algorithms. 展开更多
关键词 Frictional contact problem. Linear complementarity problem .Aggregate function ~ Interior pointalgorithm ~ Smoothing algorithm
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Extension of Compressibility-Route Cubic Equations of State and the Radial Distribution Functions at Contact to Multi-Component Hard-Sphere Mixtures
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作者 孙久勋 金柯 +1 位作者 蔡灵仓 吴强 《Communications in Theoretical Physics》 SCIE CAS CSCD 2014年第8期290-296,共7页
The equation of state(EOS) for hard-sphere fluid derived from compressibility routes of Percus-Yevick theory(PYC) is extended. The two parameters are determined by fitting well-known virial coefficients of pure fluid.... The equation of state(EOS) for hard-sphere fluid derived from compressibility routes of Percus-Yevick theory(PYC) is extended. The two parameters are determined by fitting well-known virial coefficients of pure fluid.The extended cubic EOS can be directly extended to multi-component mixtures, merely demanding the EOS of mixtures also is cubic and combining two physical conditions for the radial distribution functions at contact(RDFC) of mixtures.The calculated virial coefficients of pure fluid and predicted compressibility factors and RDFC for both pure fluid and mixtures are excellent as compared with the simulation data. The values of RDFC for mixtures with extremely large size ratio 10 are far better than the BGHLL expressions in literature. 展开更多
关键词 CUBIC equation of state radial distribution functions at contact hard SPHERE FLUIDS mixtures multi-components
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便携式睡眠监测设备的研究进展
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作者 杨红英 齐庆花 +3 位作者 王梦姣 张甜甜 刘亚东 熊雪伶 《上海纺织科技》 2026年第3期18-24,共7页
便携式睡眠监测设备凭借其方便、舒适、操作简单等优势,可在非临床环境中进行睡眠监测。从便携式睡眠监测设备的基本监测原理和发展历程出发,梳理了穿戴式/接触式和非穿戴式/非接触式两类便携式睡眠监测设备在心率监测、呼吸监测、睡眠... 便携式睡眠监测设备凭借其方便、舒适、操作简单等优势,可在非临床环境中进行睡眠监测。从便携式睡眠监测设备的基本监测原理和发展历程出发,梳理了穿戴式/接触式和非穿戴式/非接触式两类便携式睡眠监测设备在心率监测、呼吸监测、睡眠分期以及阻塞性睡眠呼吸暂停等方面的技术进展。介绍了市场上基于相关技术应用的产品,指出了各类设备在准确性、舒适性、安全性、隐私性等方面存在的问题。在此基础上,结合技术发展趋势与用户需求,对未来便携式睡眠监测设备的功能优化、技术创新及市场应用前景进行了展望,旨在为相关领域的研究提供参考。 展开更多
关键词 睡眠监测 监测设备 穿戴式设备 接触式设备 功能优化
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基于表面处理的开关柜梅花触头腐蚀抑制技术研究
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作者 冯杰 沈东明 +5 位作者 王法 金晶 盛敏杰 吴建东 谢东蔚 罗响 《电气工程学报》 北大核心 2026年第1期469-478,共10页
高湿环境引起的梅花触头表面腐蚀问题是导致高压开关柜严重故障的主要原因之一。为此,通过分析不同表面处理后梅花触头铜触片试样的腐蚀特性,结合极化曲线等测试方法,研究了梅花触头抗腐蚀性能评估新方法。试验所用腐蚀介质为5%Na OH与N... 高湿环境引起的梅花触头表面腐蚀问题是导致高压开关柜严重故障的主要原因之一。为此,通过分析不同表面处理后梅花触头铜触片试样的腐蚀特性,结合极化曲线等测试方法,研究了梅花触头抗腐蚀性能评估新方法。试验所用腐蚀介质为5%Na OH与Na HCO_(3)的混合溶液,扫描速度5 mV/s,扫描区间为±5 mV。结果表明,经过镀银后镀金处理的铜触片腐蚀电位较大,腐蚀电流较小,该现象可能与复合镀层材料的低氧化性有关;不同镀银层厚度与铜触片的抗腐蚀性能变化呈非单调趋势,在厚度为16μm时的综合抗腐蚀性能较好,固含量为10%的纳米涂层是表面疏水涂层处理的最佳处理条件,推测该现象均可能与镀层表面的均匀性与稳定性有关。单一表面处理后铜触片的抗腐蚀性能较差,采用低氧化性金属加强镀膜和复合表面处理技术能提高梅花触头不同表面区域的抗腐蚀性能。归一化评估函数F可作为梅花触头抗腐蚀性能的评估参量,制定的梅花触头保护与修复方案可为现场开关柜的运维提供理论支撑。 展开更多
关键词 高压开关柜 梅花触头 腐蚀 表面处理 评估函数
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开关柜触头温升与接触电阻变化率的函数模型构建
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作者 李景枫 《电力系统装备》 2026年第1期117-119,共3页
电力系统中开关柜运行可靠性至关重要,触头作为开关柜的核心部件,其温升与接触电阻变化的耦合关系直接影响设备安全。文章介绍了开关柜触头温升与接触电阻的技术特性及基础模型,分析了开关柜触头温升与接触电阻变化率的匹配策略,并构建... 电力系统中开关柜运行可靠性至关重要,触头作为开关柜的核心部件,其温升与接触电阻变化的耦合关系直接影响设备安全。文章介绍了开关柜触头温升与接触电阻的技术特性及基础模型,分析了开关柜触头温升与接触电阻变化率的匹配策略,并构建了开关柜触头温升与接触电阻变化率的函数模型,旨在为开关柜运维、故障预警及优化设计提供理论支撑,保障电力系统稳定运行。 展开更多
关键词 开关柜触头 温升 接触电阻变化率 函数模型 故障预警
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An New Area Function for Sharp Indenter Tips in Nanoindentation 被引量:3
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作者 刘东旭 张泰华 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2004年第3期159-164,共6页
A new area function is introduced and applied to a Berkovich tip in order tocharacterize the contact projected area between an indenter and indented material. The function canbe related directly to tip-rounding, there... A new area function is introduced and applied to a Berkovich tip in order tocharacterize the contact projected area between an indenter and indented material. The function canbe related directly to tip-rounding, thereby having obviously physical meaning. Nanoindentationexperiments are performed on a commercial Nano Indenter XP^R system. The other two area functionsintroduced by Oliver and Pharr and by Thurn and Cook respectively are involved in this paper forcomparison. By comparison from experimental results among different area functions, the indenter tipdescribed by the proposed area function here is very close to the experimental indenter. 展开更多
关键词 INDENTATION indenter contact area area function tip-rounding
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Numerical analysis of elastic coated solids in line contact 被引量:2
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作者 王廷剑 王黎钦 +1 位作者 古乐 赵小力 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第7期2470-2481,共12页
A line contact model of elastic coated solids is presented based on the influence coefficients(ICs) of surface displacement and stresses of coating-substrate system and the traditional contact model. The ICs of displa... A line contact model of elastic coated solids is presented based on the influence coefficients(ICs) of surface displacement and stresses of coating-substrate system and the traditional contact model. The ICs of displacement and stresses are obtained from their corresponding frequency response functions(FRF) by using a conversion method based on fast Fourier transformation(FFT). The contact pressure and the stress field in the subsurface are obtained by employing conjugate gradient method(CGM) and discrete convolution fast Fourier transformation(DC-FFT). Comparison of the contact pressure and subsurface stresses obtained by the numerical method with the exact analytical solutions for Hertz contact is conducted, and the results show that the numerical solution has a very high accuracy and verify the validity of the contact model. The effect of the stiffness and thickness of coatings is further numerically studied. The result shows that the effects on contact pressure and contact width are opposite for hard and soft coatings and are intensified with the increase of coating thickness; the locations of crack initiation and propagation are different for soft and hard coatings; the risk of cracks and delaminations of coatings can be brought down by improving the lubrication condition or optimizing the non-dimensional parameter h/bh. This research offers a tool to numerically analyze the problem of elastic coated solids in line contact and make the blindness and randomness of trial-type coating design less. 展开更多
关键词 coating-substrate system contact pressure stress field frequency response function influence coefficient
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Performance of n-type silicon/silver composite anode material in lithium ion batteries: A study on effect of work function matching degree 被引量:1
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作者 Guo-Jun Xu Chen-Xin Jin +6 位作者 Kai-Jie Kong Xi-Xi Yang Zhi-Hao Yue Xiao-Min Li Fu-Gen Sun Hai-Bin Huang Lang Zhou 《Chinese Physics B》 SCIE EI CAS CSCD 2018年第10期607-611,共5页
In this paper, two types of silicon(Si) particles ball-milled from n-type Si wafers, respectively, with resistivity values of 1 Ω·cm and 0.001 Ω·cm are deposited with silver(Ag). The Ag-deposited n-typ... In this paper, two types of silicon(Si) particles ball-milled from n-type Si wafers, respectively, with resistivity values of 1 Ω·cm and 0.001 Ω·cm are deposited with silver(Ag). The Ag-deposited n-type 1-Ω·cm Si particles(nl-Ag) and Ag-deposited n-type 0.001-Ω·cm Si particles(n0.001-Ag) are separately used as an anode material to assemble coin cells,of which the electrochemical performances are investigated. For the matching of work function between n-type 1-Ω·cm Si(nl) and Ag, nl-Ag shows discharge specific capacity of up to 683 mAh·g^-1 at a current density of 8.4 A·g^-1, which is40% higher than that of n0.001-Ag. Furthermore, the resistivity of nl-Ag is lower than half that of n0.001-Ag. Due to the mismatch of work function between n-type 0.001-Ω·cm Si(n0.001) and Ag, the discharge specific capacity of n0.001-Ag is 250.2 mAh·g^-1 lower than that of nl-Ag after 100 cycles. 展开更多
关键词 lithium ion battery silicon anode materials work function matching contactS
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Two kinds of contact problems in three-dimensional icosahedral quasicrystals 被引量:11
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作者 Xuefen ZHAO Xing LI Shenghu DING 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2015年第12期1569-1580,共12页
Two kinds of contact problems, i.e., the frictional contact problem and the adhesive contact problem, in three-dimensional (3D) icosahedral quasicrystals are dis- cussed by a complex variable function method. For th... Two kinds of contact problems, i.e., the frictional contact problem and the adhesive contact problem, in three-dimensional (3D) icosahedral quasicrystals are dis- cussed by a complex variable function method. For the frictional contact problem, the contact stress exhibits power singularities at the edge of the contact zone. For the adhe- sive contact problem, the contact stress exhibits oscillatory singularities at the edge of the contact zone. The numerical examples show that for the two kinds of contact problems, the contact stress exhibits singularities, and reaches the maximum value at the edge of the contact zone. The phonon-phason coupling constant has a significant effect on the contact stress intensity, while has little impact on the contact stress distribution regu- lation. The results are consistent with those of the classical elastic materials when the phonon-phason coupling constant is 0. For the adhesive contact problem, the indentation force has positive correlation with the contact displacement, but the phonon-phason cou- pling constant impact is barely perceptible. The validity of the conclusions is verified. 展开更多
关键词 three-dimensional (3D) icosahedral quasicrystal Riemann-Hilbert problem contact problem SINGULARITY complex variable function method
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Nondestructive measurement of thermal contact resistance for the power vertical double-diffused metal-oxide-semiconductor
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作者 李睿 郭春生 +3 位作者 冯士维 石磊 朱慧 王琳 《Chinese Physics B》 SCIE EI CAS CSCD 2015年第7期384-386,共3页
To obtain thermal contact resistance(TCR) between the vertical double-diffused metal-oxide-semiconductor(VDMOS) and the heat sink, we derived the relationship between the total thermal resistance and the contact f... To obtain thermal contact resistance(TCR) between the vertical double-diffused metal-oxide-semiconductor(VDMOS) and the heat sink, we derived the relationship between the total thermal resistance and the contact force imposed on the VDMOS. The total thermal resistance from the chip to the heat sink is measured under different contact forces, and the TCR can be extracted nondestructively from the derived relationship. Finally, the experimental results are compared with the simulation results. 展开更多
关键词 thermal contact resistance nondestructive measurement method structure function contact pressure
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Momentum distribution and non-local high order correlation functions of 1D strongly interacting Bose gas
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作者 EJKP Nandani Xi-Wen Guan 《Chinese Physics B》 SCIE EI CAS CSCD 2018年第7期138-151,共14页
The Lieb-Liniger model is a prototypical integrable model and has been turned into the benchmark physics in theoretical and numerical investigations of low-dimensional quantum systems. In this note, we present various... The Lieb-Liniger model is a prototypical integrable model and has been turned into the benchmark physics in theoretical and numerical investigations of low-dimensional quantum systems. In this note, we present various methods for calculating local and nonlocal M-particle correlation functions, momentum distribution, and static structure factor. In particular, using the Bethe ansatz wave function of the strong coupling Lieb-Liniger model, we analytically calculate the two-point correlation function, the large moment tail of the momentum distribution, and the static structure factor of the model in terms of the fractional statistical parameter a = 1 - 2/γ, where γ, is the dimensionless interaction strength. We also discuss the Tan's adiabatic relation and other universal relations for the strongly repulsive Lieb-Liniger model in terms of the fractional statistical parameter. 展开更多
关键词 correlation function momentum distributions structure factor contact
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The Classical Hall Effect in Multiply-Connected Plane Regions Part I: Topologies with Stream Function 被引量:1
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作者 Udo Ausserlechner 《Journal of Applied Mathematics and Physics》 2019年第9期1968-1996,共29页
Typical Hall plates for practical magnetic field sensing purposes are plane, simply-connected regions with peripheral contacts. Their output voltage is the sum of even and odd functions of the applied magnetic field. ... Typical Hall plates for practical magnetic field sensing purposes are plane, simply-connected regions with peripheral contacts. Their output voltage is the sum of even and odd functions of the applied magnetic field. They are commonly called offset and Hall voltage. Contemporary smart Hall sensor circuits extract the Hall voltage via spinning current Hall probe schemes, thereby cancelling out the offset very efficiently. The magnetic field response of such Hall plates can be computed via the electric potential or via the stream function. Conversely, Hall plates with holes show new phenomena: 1) the stream function exists only for a limited class of multiply-connected domains, and 2) a sub-class of 1) behaves like a Hall/Anti-Hall bar configuration, i.e., no Hall voltage appears between any two points on the hole boundary if current contacts are on their outer boundary. The paper studies the requirements under which these effects occur. Canonical cases of simply and doubly connected domains are computed analytically. The focus is on 2D multiply-connected Hall plates where all boundaries are insulating and where all current contacts are point sized. 展开更多
关键词 Anti-Hall Bar DOUBLY CONNECTED Geometry Factor Hall Plate Multiply CONNECTED Non-Peripheral contacts Reverse Magnetic Field RECIPROCITY Stream function
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Contact Pressure between the Outside Surface of the Sealer and Cylinder Wall
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作者 Sc. D. Elkhan M. Abbasov Kaklik O. Rustamova Aynur O. Darishova 《Journal of Applied Mathematics and Physics》 2020年第2期349-366,共18页
Character of contract pressure distribution between the outside surface of the sealing material and rigid cylinder wall depending on geometrical sizes and mechanical properties of a sealer under its unilateral compres... Character of contract pressure distribution between the outside surface of the sealing material and rigid cylinder wall depending on geometrical sizes and mechanical properties of a sealer under its unilateral compression, is defined. The magnitude of the axial load for achieving tightness is determined. The dependence between the magnitude of the axial load necessary for achieving tightness and geometrical sizes is determined. It is shown that with a decrease in the height of the sealing element, the axial load necessary for achieving tightness greatly increases. Threshold height of the sealer, above which contact pressure depends little on the magnitude of the axial load, is defined. The stress-strain state of the sealing element is defined with regard to viscous-elastic properties of its material. It is shown that this greatly influences its sealing ability. 展开更多
关键词 contact Pressure SEALING ELEMENT BOUNDARY CONDITION Potential Energy functional
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Assessment of Non-Contact Measurement Using a Microwave Sensor to Jugular Venous Pulse Monitoring
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作者 Satoshi Suzuki Masaaki Hoshiga +1 位作者 Kentaro Kotani Takafumi Asao 《Journal of Biomedical Science and Engineering》 2021年第3期94-102,共9页
The jugular venous pulse (JVP) waveform provides an insight into right heart function, and its assessment is important in patients with heart failure. However, the conventional pulse-transducer (contact) method for mo... The jugular venous pulse (JVP) waveform provides an insight into right heart function, and its assessment is important in patients with heart failure. However, the conventional pulse-transducer (contact) method for monitoring this waveform is not frequently used because it requires a high degree of skill. The aim of this study was to confirm the effectiveness of a prototype non-contact system that employs microwave radar (24 GHz, 7 mW;non-contact system) for JVP measurement. Experiments were conducted on eight healthy male volunteers (21.88 ± 0.99 years). JVP measurements were compared between the conventional contact method and the proposed non-contact method. Change in JVP waveform was measured in response to an angle of reclining in five steps from the supine position to 75<span style="white-space:nowrap;">°</span> of elevation. The obtained JVP measurements were similar between the two methods. Because in the non-contact method the faint pulsation of the JVP is not suppressed by the pressure of a sensor placed on the skin, the prototype microwave radar system is particularly suitable for evaluating the JVP waveform. 展开更多
关键词 Microwave Radar Non-contact Monitoring Jugular Venous Pressure Right Heart function
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触液神经元分化为运动神经元促进脊髓损伤小鼠运动功能恢复
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作者 唐旻 上官泽宇 +2 位作者 李琦哲 谭伟 李青 《中国组织工程研究》 北大核心 2026年第19期4965-4971,共7页
背景:细胞移植是修复脊髓损伤的有效方式之一,课题组研究发现移植的触液神经元可以存活,促进小鼠脊髓损伤后运动功能改善。但是,移植的触液神经元在体内是否分化为有功能的神经元,从而促进脊髓损伤运动功能恢复尚不清楚。目的:探究触液... 背景:细胞移植是修复脊髓损伤的有效方式之一,课题组研究发现移植的触液神经元可以存活,促进小鼠脊髓损伤后运动功能改善。但是,移植的触液神经元在体内是否分化为有功能的神经元,从而促进脊髓损伤运动功能恢复尚不清楚。目的:探究触液神经元体内移植后是否分化为有功能的神经元促进脊髓损伤运动功能恢复。方法:从出生24 h内的C57BL/6乳鼠颈髓中分离含有触液神经元的原代细胞进行贴壁培养,使用融合多模态成像基因的慢病毒转染细胞,并通过嘌呤霉素筛选和纯化触液神经元,用含血清分化培养基诱导触液神经元分化,通过免疫荧光检测触液神经元分化后神经元标记物NeuN和运动神经元标记物ChAT的表达。将30只C57BL/6小鼠随机分为3组,移植组及PBS组通过钳夹法建立T10节段脊髓损伤模型,假手术组仅打开椎板;脊髓损伤后1周,移植组原位移植触液神经元,PBS组注入等量PBS。在移植后第1,4,8周,免疫荧光检测脊髓组织运动神经元标记物ChAT的表达;移植后第8周免疫荧光检测突触标记物SYN、抑制性递质标记物GAD65/67和兴奋性递质标记物vGLUT1的表达,苏木精-伊红染色观察脊髓组织形态,BMS运动功能评分、足迹分析评估小鼠运动功能恢复情况。结果与结论:(1)触液神经元体外表达神经干细胞特性,可分化为运动神经元;(2)移植触液神经元可以在小鼠体内长期存活、分化为运动神经元;(3)触液神经元分化为运动神经元的比例在第8周最高(P<0.000 1);(4)移植后8周,触液神经元与突触标记物SYN、抑制性递质标记物GAD65/67、兴奋性递质标记物vGLUT1荧光共表达,说明移植触液神经元与宿主神经元形成突触联系;(5)PBS组小鼠的BMS评分始终低于移植组(P<0.001),移植组小鼠足迹较为协调,仅有足趾拖拽,PBS组小鼠存在明显的下肢拖拽;(6)苏木精-伊红染色显示PBS组小鼠损伤区域形成大片空洞,移植组小鼠损伤区域空洞减小。以上结果表明,移植触液神经元在体外及体内均可分化为运动神经元,并形成突触连接,从而改善脊髓损伤小鼠的运动功能。 展开更多
关键词 脊髓损伤 触液神经元 神经干细胞 运动神经元 移植 突触重建 运动功能恢复 多模态成像基因慢病毒
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