Bone tissue engineering may be hindered by underlying osteoporosis because of a decreased osteogenic ability of autologous seed cells and an unfavorably changed microenvironment in these patients. Epigenetic regulatio...Bone tissue engineering may be hindered by underlying osteoporosis because of a decreased osteogenic ability of autologous seed cells and an unfavorably changed microenvironment in these patients. Epigenetic regulation plays an important role in the developmental origins of osteoporosis; however, few studies have investigated the potential of epigenetic therapy to improve or rescue the osteogenic ability of bone marrow mesenchymal stem cells(BMMSCs) under osteoporotic conditions. Here, we investigated pargyline, an inhibitor of lysine-specific demethylase 1(LSD1), which mainly catalyzes the demethylation of the di- and mono-methylation of H3K4. We demonstrated that 1.5 mmol·Lpargyline was the optimal concentration for the osteogenic differentiation of human BMMSCs. Pargyline rescued the osteogenic differentiation ability of mouse BMMSCs under osteoporotic conditions by enhancing the dimethylation level of H3K4 at the promoter regions of osteogenesis-related genes. Moreover, pargyline partially rescued or prevented the osteoporotic conditions in aged or ovariectomized mouse models, respectively. By introducing the concept of epigenetic therapy into the field of osteoporosis, this study demonstrated that LSD1 inhibitors could improve the clinical practice of MSC-based bone tissue engineering and proposes their novel use to treat osteoporosis.展开更多
Bismuth sulfide(Bi_(2)S_(3))is a promising sensing material owing to its unique properties like a narrow bandgap,a large number of surface-adsorption sites,and excellent electrical properties.However,the unsatisfactor...Bismuth sulfide(Bi_(2)S_(3))is a promising sensing material owing to its unique properties like a narrow bandgap,a large number of surface-adsorption sites,and excellent electrical properties.However,the unsatisfactory sensitivity precludes its application in high-performance sensors to detect H_(2)S.Herein,a Bi_(2)S_(3)/ZnS heterostructure was constructed as a sensing material to further improve its sensitivity toward H_(2)S by modulating light or dark conditions.展开更多
基金supported by grants from the National Natural Science Foundation of China(81200763 to WG and 81070809 to YZ)the Program for New Century Excellent Talents(NCET)at the University from Ministry of Education of China(NCET-11-0026)+1 种基金the PKU School of Stomatology for Talented Young Investigators(PKUSS20150107)the Construction Program for the National Key Clinical Specialty from the National Health and Family Planning Commission of China(2011)
文摘Bone tissue engineering may be hindered by underlying osteoporosis because of a decreased osteogenic ability of autologous seed cells and an unfavorably changed microenvironment in these patients. Epigenetic regulation plays an important role in the developmental origins of osteoporosis; however, few studies have investigated the potential of epigenetic therapy to improve or rescue the osteogenic ability of bone marrow mesenchymal stem cells(BMMSCs) under osteoporotic conditions. Here, we investigated pargyline, an inhibitor of lysine-specific demethylase 1(LSD1), which mainly catalyzes the demethylation of the di- and mono-methylation of H3K4. We demonstrated that 1.5 mmol·Lpargyline was the optimal concentration for the osteogenic differentiation of human BMMSCs. Pargyline rescued the osteogenic differentiation ability of mouse BMMSCs under osteoporotic conditions by enhancing the dimethylation level of H3K4 at the promoter regions of osteogenesis-related genes. Moreover, pargyline partially rescued or prevented the osteoporotic conditions in aged or ovariectomized mouse models, respectively. By introducing the concept of epigenetic therapy into the field of osteoporosis, this study demonstrated that LSD1 inhibitors could improve the clinical practice of MSC-based bone tissue engineering and proposes their novel use to treat osteoporosis.
基金supported by the National Natural Science Foundation of China(No.52072093 and 51802058)the National Key Research and Development Project of China(No.2019YFA0705201)+1 种基金the Joint Funds of Natural Science Foundation of Heilongjiang Province(No.LH2021E053)the Fundamental Research Funds for the Central Universities(No.2022FRFK02015).
文摘Bismuth sulfide(Bi_(2)S_(3))is a promising sensing material owing to its unique properties like a narrow bandgap,a large number of surface-adsorption sites,and excellent electrical properties.However,the unsatisfactory sensitivity precludes its application in high-performance sensors to detect H_(2)S.Herein,a Bi_(2)S_(3)/ZnS heterostructure was constructed as a sensing material to further improve its sensitivity toward H_(2)S by modulating light or dark conditions.
