When the stagnation temperature of a perfect gas increases, the specific heats and their ratio do not remain constant any more and start to vary with this temperature. The gas remains perfect, its state equation remai...When the stagnation temperature of a perfect gas increases, the specific heats and their ratio do not remain constant any more and start to vary with this temperature. The gas remains perfect, its state equation remains always valid, except it will name in more calorically imperfect gas or gas at High Temperature. The goal of this research is to trace the profiles of the supersonic plug nozzle when this stagnation temperature is taken into account, lower than the threshold of dissociation of the molecules, by using the new formula of the Prandtl Meyer function, and to have for each exit Mach number, several nozzles shapes by changing the value of this temperature. A study on the error given by the PG （perfect gas） model compared to our model at high temperature is presented. The comparison is made with the case of a calorically perfect gas aiming to give a limit of application of this model. The application is for the air.展开更多
This study,based on the theory of shifting essence and changing Qi in Inner Canon of Huangdi,deeply explores the theoretical basis,operation method of Conception Vessel Dredging Therapy and its influence on human heal...This study,based on the theory of shifting essence and changing Qi in Inner Canon of Huangdi,deeply explores the theoretical basis,operation method of Conception Vessel Dredging Therapy and its influence on human health.Through case analysis and the statistical analysis of medical records during the pandemic,it reveals the remarkable effects of this method in regulating the endocrine system,improving metabolism,and relieving physical and mental diseases.It provides a theoretical and practical basis for the application of traditional Chinese medicine anti-aging therapy in the modern health field,and helps to explore new ways to delay aging and improve physical and mental health.展开更多
Objective To explore the effect of electroacupuncture at conception vessel and governor vessel on correlative signals of inflammatory response stressinjury-repair in cerebral ischemia reperfusion rats. Methods A total...Objective To explore the effect of electroacupuncture at conception vessel and governor vessel on correlative signals of inflammatory response stressinjury-repair in cerebral ischemia reperfusion rats. Methods A total of 216 rats were included in the experiment, of which 192 rats were randomly divided into four groups, including sham operation group（group A）, model group（group B）, governor vessel group（group C）, and conception vessel and governor vessel group（group D）, 48 rats in each group. The rats in each group were further divided into six sub-groups, including 12 h group, 24 h group, 48 h group, 72 h group, 96 h group and 144 h group. MCAO operation course was simulated in group A, and the common carotid artery, internal carotid artery and external carotid artery on the right side were exposed, without insertion of suture or treatment. After completion of modeling in group B, treatment was not conducted. After completion of modeling in group C, ＂Shuǐgōu（水沟 GV 26）＂, ＂Bǎihuì（百会 GV 20）＂ and ＂Dàzhuī（大椎 GV 14）＂ of governor vessel were selected, and electroacupuncture device was connected with ＂GV 14＂ and ＂GV 20＂. After completion of modeling in group D, ＂GV 26＂, ＂GV 20＂ and ＂GV 14＂ of governor vessel, and ＂Qìhǎi（气海 CV 6）＂, ＂Guānyuán（关元 CV 4）＂ and ＂Chéngjiāng（承浆 CV 24）＂ of conception vessel were selected; electroacupuncture device was connected with ＂GV 14＂ and ＂GV 20＂, ＂CV 4＂ and ＂CV 6＂. Neurological severity score was conducted.Enzyme linked immunosorbent assay（ELISA） was applied to determine the concentration of peripheral serum adrenocorticotropic hormone（ACTH） of rats, and immunohistochemical method was adopted to detect the expression levels of tumor necrosis factor α（TNF-α） and transforming growth factor β1（TGF-β1） in brain tissue of rats. The other 24 rats were also randomly divided into four groups mentioned above, 6 rats in each group, to detect cerebral ischemie volume. Results Compared with group B, the ischemia volume in brain tissue at 144 h after cerebral ischemia reperfusion decreased significantly in group C and group D（P〈0.05）, and the ischemia volume in group D was less than that in group C（P〈0.05）. The neurological impairment scores of group C and group D at all time points were lower than those of group B, and the differences were statistically significant（all P〈0.05）. The scores of group D at all time points were lower than those of group C, and the differences since 24 h were statistically significant（all P〈0.05）. Compared with group B, the excessive expression of ACTH was suppressed dynamically and generally in group C and group D（P〈0.05）; the expression level of TNF-α was down-regulated and TGF-β1 was up-regulated as well in group C and group D（P〈0.05）. The effect in group D was superior to that in group C. Conclusion Electroacupuncture at conception vessel and governor vessel can protect brain through the intervention of the expression of correlative signals of inflammatory response stress-injury-repair of cerebral ischemia reperfusion.展开更多
In modern Chinese style garden, the designer could express the emotions in garden creation to produce artistic conception with landscape plants, which could make the sightseers desire to integrate with the nature and ...In modern Chinese style garden, the designer could express the emotions in garden creation to produce artistic conception with landscape plants, which could make the sightseers desire to integrate with the nature and led to sympathizing with the concept, thereby motivating and arousing people＇s emotion to return to and close to nature. Through the 6 specific characteristics of ＂shape, color, aroma, shadow, sound and art＂ of landscape plants, some ways to build artistic conception in Chinese style garden were introduced in this study to provide references for the creation of artistic conception creation through senses in the future landscaping design.展开更多
This study explored the origin and development of modern garden city,by integrating concepts of world modern garden city and characteristics of Chengdu cultures,analyzed the application of its aesthetic value and the ...This study explored the origin and development of modern garden city,by integrating concepts of world modern garden city and characteristics of Chengdu cultures,analyzed the application of its aesthetic value and the design of its artistic conception.It was found that Chengdu City had high aesthetic value such as a large area of green spaces and unique recreational cultures,as well as a great potential of unifying idyllic images and natural cultures.To design Chengdu into a world modern garden city,the planning of its urban green space system should be taken as the foundation,"one group and three districts" as the construction system,urban folk cultures,logistics and traffic,information and other such networks as the support to achieve the "beauty of nature,social equity and urban-rural integration".展开更多
To better inherit traditional culture in creating artistic conception in modern gardens and develop the style and function of garden artistic conception,this study summarized various creation techniques of traditional...To better inherit traditional culture in creating artistic conception in modern gardens and develop the style and function of garden artistic conception,this study summarized various creation techniques of traditional garden artistic conceptions from the perspective of design thought and conception of classical Chinese gardens.It was put forward that the creation of artistic conception in modern gardens should be human-centered in a great culture context;and meanwhile,aesthetic habits of modern people should be respected and traditional thoughts should be developed to enrich the connotation of garden artistic conception design.展开更多
Elementary knowledge of classical Chinese gardens was introduced,its history was studied in 3 stages:initial phase,development phase and prosperous phase,component system of classical Chinese gardens was introduced fr...Elementary knowledge of classical Chinese gardens was introduced,its history was studied in 3 stages:initial phase,development phase and prosperous phase,component system of classical Chinese gardens was introduced from different perspectives,cultural background and specific demonstrations of its artistic conceptions were expounded as key points,and it was proposed that its artistic conceptions were actually reverence and respect for the nature,and also expressions of various philosophical doctrines and religious cultures.On this basis,construction techniques and connotations of artistic conception were researched as well as its components and expression ways.It was proposed that construction elements of artistic conceptions included buildings,hill stones,water and plants;image-building in the landscaping should be stressed,and artistic conception integrated into key points to bring out the theme,garden scenes and paintings combined.This study aimed at providing references for relevant researches through introducing classical Chinese gardens and their artistic conceptions.展开更多
目的:应用Walker-Avant经典概念分析法界定孤独症谱系障碍病人社交伪装的概念内涵。方法:使用关键词检索PubMed、Web of Science、Cochrane Library、OVID、EMbase、中国知网、维普中文期刊服务平台、万方数据知识服务平台、中国生物医...目的:应用Walker-Avant经典概念分析法界定孤独症谱系障碍病人社交伪装的概念内涵。方法:使用关键词检索PubMed、Web of Science、Cochrane Library、OVID、EMbase、中国知网、维普中文期刊服务平台、万方数据知识服务平台、中国生物医学数据库等中英文数据库,并进行手工检索相关文献,系统筛选了从建库至2025年3月1日公开发表的与孤独症谱系障碍社交伪装相关的文献,并采用Walker-Avant经典概念分析法进行分析。结果:共纳入58篇文献,其中孤独症谱系障碍病人社交伪装的定义属性包括掩饰特征、补偿策略、同化行为以及内化耻辱;前因包括社会文化因素、个体心理因素、人口学因素以及社会环境因素;后果包括对孤独症病人的积极影响和消极影响,分别是促进社会适应、损害心理健康以及阻碍社会支持和人际关系。结论:通过对孤独症谱系障碍社交伪装进行概念分析,有利于医护人员精准把握孤独症病人的相关病症、优化其诊疗方式,理解病人的社交策略和行为举止,为个性化干预提供相关依据。展开更多
Effective teaching has always been a goal pursued by teachers as a part of education both from the western and eastern society,for it to some extend is considered an effective way to improve society.In this essay,both...Effective teaching has always been a goal pursued by teachers as a part of education both from the western and eastern society,for it to some extend is considered an effective way to improve society.In this essay,both Chinese and western conceptions of teaching and learning will be discussed.In doing so,their differences and similarities can be seen clearly,which will help the cross-cultural educators to understand their teaching environment and learning styles.展开更多
目的:厘清植物性饮食的概念内涵,为植物性饮食的研究和实践提供理论指导。方法:系统检索中国知网、万方数据库、维普数据库、中国生物医学文献数据库、PubMed、EMbase、Web of Science、Scopus数据库中与植物性饮食有关的文献,检索时限...目的:厘清植物性饮食的概念内涵,为植物性饮食的研究和实践提供理论指导。方法:系统检索中国知网、万方数据库、维普数据库、中国生物医学文献数据库、PubMed、EMbase、Web of Science、Scopus数据库中与植物性饮食有关的文献,检索时限为建库至2024年12月30日。依据Walker及Avant概念分析法进行植物性饮食的概念分析。结果:共纳入48篇文献。植物性饮食包括食物来源以植物为主、多样化的食物选择、可能限制或排除动物产品3个定义属性。前因包括自身因素、社会因素、经济因素;后果包括健康益处、健康潜在风险、环境影响。结论:通过概念分析明确了植物性饮食概念属性。未来可针对植物性饮食的积极影响展开研究,提高公众接受度,并对植物性饮食的个性化干预进行更为系统、深入的研究。展开更多
Mesenchymal stem cell transplantation is a novel means of treating cerebral ischemia/reper- fusion, and can promote angiogenesis and neurological functional recovery. Acupuncture at Conception and Governor vessels als...Mesenchymal stem cell transplantation is a novel means of treating cerebral ischemia/reper- fusion, and can promote angiogenesis and neurological functional recovery. Acupuncture at Conception and Governor vessels also has positive effects as a treatment for cerebral ischemia/ reperfusion. Therefore, we hypothesized that electro-acupuncture at Conception and Governor vessels plus mesenchymal stem cell transplantation may have better therapeutic effects on the promotion of angiogenesis and recovery of neurological function than either treatment alone. In the present study, human umbilical cord blood-derived mesenchymal stem cells were isolated, cultured, identified and intracranially transplanted into the striatum and subcortex of rats at 24 hours following cerebral ischemia/reperfusion. Subsequently, rats were electro-acupunctured at Conception and Governor vessels at 24 hours after transplantation. Modified neurological severity scores and immunohistochemistry findings revealed that the combined interventions of electro-acupuncture and mesenchymal stem cell transplantation clearly improved neurological impairment and up-regulated vascular endothelial growth factor expression around the isch- emic focus. The combined intervention provided a better outcome than mesenchymal stem cell transplantation alone. These findings demonstrate that electro-acupuncture at Conception and Governor vessels and mesenchymal stem cell transplantation have synergetic effects on promot- ing neurological function recovery and angiogenesis in rats after cerebral ischemia/reperfusion.展开更多
OBJECTIVE: To investigate the combinatorial effects of conception and governor vessel electroacupuncture(EA) and human umbilical cord blood-derived mesenchymal stem cells(HUCB-MSCs) on pathomorphologic lesion and cell...OBJECTIVE: To investigate the combinatorial effects of conception and governor vessel electroacupuncture(EA) and human umbilical cord blood-derived mesenchymal stem cells(HUCB-MSCs) on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion. METHODS: With the HUCB-MSCs isolated, cultured and identified and the models of cerebral ischemia-reperfusion established, the HUCB-MSCs of passage three were intracranially transplanted and the EA at conception and governor vessels was applied. The pathomorphologic lesion by hematoxylin-eosin staining and the cellular apoptosis by terminal deoxynucleotidyl transferase-mediated nick-end labeling method around the ischemic focus were observed. RESULTS: The cultured adherent HUCB-MSCs exhibited a spindle shape and expressed MSC-specific markers, with the cell purity and proliferation rate significantly increasing after the primary passage. HE staining showed that there were no pathological changes observed in the sham surgery group. However, in the PBS transplantation group, degeneration and necrosis of a great number of nerve cells were seen. In both the HUCB-MSCs transplantation group and the HUCB-MSCs transplantation + EA group, reparative changes of the pathomorphism of the tissue were found. Both combination treatment and simple MSCs treatment were able to improve the pathomorphorlogic lesion following cerebral ischemia and reduce the abnormal TUNEL-positive numbers, with former better than latter. CONCLUSION: HUCB-MSCs improve pathological lesions and inhibit the cellular apoptosis around the cerebral ischemic area. EA at conception and governor vessels also improve pathological lesion and inhibit the cellular apoptosis in rats treated with HUCB-MSCs transplantation, which effects were superior to that of simple HUCB-MSCs transplantation.展开更多
Approximately 30% of pregnancies after implantation end up in spontaneous abortions, and 50% of them are caused by chromosomal abnormalities. However, the spectrum of genomic copy number variants （CNVs） in products ...Approximately 30% of pregnancies after implantation end up in spontaneous abortions, and 50% of them are caused by chromosomal abnormalities. However, the spectrum of genomic copy number variants （CNVs） in products of conception （POC） and the underlying gene- dosage-sensitive mechanisms causing spontaneous abortions remain largely unknown. In this study, array comparative genornic hybridiza- tion （aCGH） analysis was performed as a salvage procedure for 128 POC culture failure （POC-CF） samples and as a supplemental procedure for 106 POC normal karyotype （POC-NK） samples. Chromosomal abnormalities were detected in 10% of POC-CF and pathogenic CNVs were detected in 3.9% of POC-CF and 5.7% of POC-NK samples. Compiled results from this study and relevant case series through a literature review demonstrated an abnormality detection rate （ADR） of 35% for chromosomal abnormalities in POC-CF samples, 3.7% for pathogenic CNVs in POC-CF samples, and 4.6% for pathogenic CNVs in POC-NK samples. Ingenuity Pathway Analysis （IPA） was performed on the genes from pathogenic CNVs found in POC samples. The denoted primary gene networks suggested that apoptosis and cell proliferation pathways are involved in miscarriage. In summary, a similar spectrum of cytogenomic abnormalities was observed in POC culture success and POC-CF samples. A threshold effect correlating the number of dosage-sensitive genes in a chromosome with the observed frequency of autosomai trisomy is proposed. A rationalized approach using firstly fluorescence in situ hybridization （FISH） testing with probes of chromosomes X/Y/ 18, 13/21, and 15/16/22 for common aneuploidies and polyploidies and secondly aCGH for other cytogenomic abnormalities is recommended for POC-CF samples.展开更多
文摘When the stagnation temperature of a perfect gas increases, the specific heats and their ratio do not remain constant any more and start to vary with this temperature. The gas remains perfect, its state equation remains always valid, except it will name in more calorically imperfect gas or gas at High Temperature. The goal of this research is to trace the profiles of the supersonic plug nozzle when this stagnation temperature is taken into account, lower than the threshold of dissociation of the molecules, by using the new formula of the Prandtl Meyer function, and to have for each exit Mach number, several nozzles shapes by changing the value of this temperature. A study on the error given by the PG （perfect gas） model compared to our model at high temperature is presented. The comparison is made with the case of a calorically perfect gas aiming to give a limit of application of this model. The application is for the air.
基金Supported by Natural Science Foundation of China,Taoist Qi Healing the World Project(Project No.:71971359).
文摘This study,based on the theory of shifting essence and changing Qi in Inner Canon of Huangdi,deeply explores the theoretical basis,operation method of Conception Vessel Dredging Therapy and its influence on human health.Through case analysis and the statistical analysis of medical records during the pandemic,it reveals the remarkable effects of this method in regulating the endocrine system,improving metabolism,and relieving physical and mental diseases.It provides a theoretical and practical basis for the application of traditional Chinese medicine anti-aging therapy in the modern health field,and helps to explore new ways to delay aging and improve physical and mental health.
基金Supported by Shenzhen Municipal Scientific and Technological Innovation Committee:JCYJ 20140408152909270Project of Shenzhen Municipal Key Laboratory of Technical Research and Development Fund Conditions and Platform Construction Plan:CXB 201111250113A
文摘Objective To explore the effect of electroacupuncture at conception vessel and governor vessel on correlative signals of inflammatory response stressinjury-repair in cerebral ischemia reperfusion rats. Methods A total of 216 rats were included in the experiment, of which 192 rats were randomly divided into four groups, including sham operation group（group A）, model group（group B）, governor vessel group（group C）, and conception vessel and governor vessel group（group D）, 48 rats in each group. The rats in each group were further divided into six sub-groups, including 12 h group, 24 h group, 48 h group, 72 h group, 96 h group and 144 h group. MCAO operation course was simulated in group A, and the common carotid artery, internal carotid artery and external carotid artery on the right side were exposed, without insertion of suture or treatment. After completion of modeling in group B, treatment was not conducted. After completion of modeling in group C, ＂Shuǐgōu（水沟 GV 26）＂, ＂Bǎihuì（百会 GV 20）＂ and ＂Dàzhuī（大椎 GV 14）＂ of governor vessel were selected, and electroacupuncture device was connected with ＂GV 14＂ and ＂GV 20＂. After completion of modeling in group D, ＂GV 26＂, ＂GV 20＂ and ＂GV 14＂ of governor vessel, and ＂Qìhǎi（气海 CV 6）＂, ＂Guānyuán（关元 CV 4）＂ and ＂Chéngjiāng（承浆 CV 24）＂ of conception vessel were selected; electroacupuncture device was connected with ＂GV 14＂ and ＂GV 20＂, ＂CV 4＂ and ＂CV 6＂. Neurological severity score was conducted.Enzyme linked immunosorbent assay（ELISA） was applied to determine the concentration of peripheral serum adrenocorticotropic hormone（ACTH） of rats, and immunohistochemical method was adopted to detect the expression levels of tumor necrosis factor α（TNF-α） and transforming growth factor β1（TGF-β1） in brain tissue of rats. The other 24 rats were also randomly divided into four groups mentioned above, 6 rats in each group, to detect cerebral ischemie volume. Results Compared with group B, the ischemia volume in brain tissue at 144 h after cerebral ischemia reperfusion decreased significantly in group C and group D（P〈0.05）, and the ischemia volume in group D was less than that in group C（P〈0.05）. The neurological impairment scores of group C and group D at all time points were lower than those of group B, and the differences were statistically significant（all P〈0.05）. The scores of group D at all time points were lower than those of group C, and the differences since 24 h were statistically significant（all P〈0.05）. Compared with group B, the excessive expression of ACTH was suppressed dynamically and generally in group C and group D（P〈0.05）; the expression level of TNF-α was down-regulated and TGF-β1 was up-regulated as well in group C and group D（P〈0.05）. The effect in group D was superior to that in group C. Conclusion Electroacupuncture at conception vessel and governor vessel can protect brain through the intervention of the expression of correlative signals of inflammatory response stress-injury-repair of cerebral ischemia reperfusion.
基金Supported by the Soft Science Project of the Science and Technology Agency of Jiangxi Province(20133BBA10017)the Planning Project for Arts and Social Sciences of Jiangxi Province~~
文摘In modern Chinese style garden, the designer could express the emotions in garden creation to produce artistic conception with landscape plants, which could make the sightseers desire to integrate with the nature and led to sympathizing with the concept, thereby motivating and arousing people＇s emotion to return to and close to nature. Through the 6 specific characteristics of ＂shape, color, aroma, shadow, sound and art＂ of landscape plants, some ways to build artistic conception in Chinese style garden were introduced in this study to provide references for the creation of artistic conception creation through senses in the future landscaping design.
基金Supported by Protection of Linpan Resources and Demonstration Protection of Bamboo Industry Chain Cultivation in West Sichuan under the Supervision of Sichuan Provincial Department of Science and Technology (2009FZ0028)~~
文摘This study explored the origin and development of modern garden city,by integrating concepts of world modern garden city and characteristics of Chengdu cultures,analyzed the application of its aesthetic value and the design of its artistic conception.It was found that Chengdu City had high aesthetic value such as a large area of green spaces and unique recreational cultures,as well as a great potential of unifying idyllic images and natural cultures.To design Chengdu into a world modern garden city,the planning of its urban green space system should be taken as the foundation,"one group and three districts" as the construction system,urban folk cultures,logistics and traffic,information and other such networks as the support to achieve the "beauty of nature,social equity and urban-rural integration".
文摘To better inherit traditional culture in creating artistic conception in modern gardens and develop the style and function of garden artistic conception,this study summarized various creation techniques of traditional garden artistic conceptions from the perspective of design thought and conception of classical Chinese gardens.It was put forward that the creation of artistic conception in modern gardens should be human-centered in a great culture context;and meanwhile,aesthetic habits of modern people should be respected and traditional thoughts should be developed to enrich the connotation of garden artistic conception design.
文摘Elementary knowledge of classical Chinese gardens was introduced,its history was studied in 3 stages:initial phase,development phase and prosperous phase,component system of classical Chinese gardens was introduced from different perspectives,cultural background and specific demonstrations of its artistic conceptions were expounded as key points,and it was proposed that its artistic conceptions were actually reverence and respect for the nature,and also expressions of various philosophical doctrines and religious cultures.On this basis,construction techniques and connotations of artistic conception were researched as well as its components and expression ways.It was proposed that construction elements of artistic conceptions included buildings,hill stones,water and plants;image-building in the landscaping should be stressed,and artistic conception integrated into key points to bring out the theme,garden scenes and paintings combined.This study aimed at providing references for relevant researches through introducing classical Chinese gardens and their artistic conceptions.
文摘目的:应用Walker-Avant经典概念分析法界定孤独症谱系障碍病人社交伪装的概念内涵。方法:使用关键词检索PubMed、Web of Science、Cochrane Library、OVID、EMbase、中国知网、维普中文期刊服务平台、万方数据知识服务平台、中国生物医学数据库等中英文数据库,并进行手工检索相关文献,系统筛选了从建库至2025年3月1日公开发表的与孤独症谱系障碍社交伪装相关的文献,并采用Walker-Avant经典概念分析法进行分析。结果:共纳入58篇文献,其中孤独症谱系障碍病人社交伪装的定义属性包括掩饰特征、补偿策略、同化行为以及内化耻辱;前因包括社会文化因素、个体心理因素、人口学因素以及社会环境因素;后果包括对孤独症病人的积极影响和消极影响,分别是促进社会适应、损害心理健康以及阻碍社会支持和人际关系。结论:通过对孤独症谱系障碍社交伪装进行概念分析,有利于医护人员精准把握孤独症病人的相关病症、优化其诊疗方式,理解病人的社交策略和行为举止,为个性化干预提供相关依据。
文摘Effective teaching has always been a goal pursued by teachers as a part of education both from the western and eastern society,for it to some extend is considered an effective way to improve society.In this essay,both Chinese and western conceptions of teaching and learning will be discussed.In doing so,their differences and similarities can be seen clearly,which will help the cross-cultural educators to understand their teaching environment and learning styles.
文摘目的:厘清植物性饮食的概念内涵,为植物性饮食的研究和实践提供理论指导。方法:系统检索中国知网、万方数据库、维普数据库、中国生物医学文献数据库、PubMed、EMbase、Web of Science、Scopus数据库中与植物性饮食有关的文献,检索时限为建库至2024年12月30日。依据Walker及Avant概念分析法进行植物性饮食的概念分析。结果:共纳入48篇文献。植物性饮食包括食物来源以植物为主、多样化的食物选择、可能限制或排除动物产品3个定义属性。前因包括自身因素、社会因素、经济因素;后果包括健康益处、健康潜在风险、环境影响。结论:通过概念分析明确了植物性饮食概念属性。未来可针对植物性饮食的积极影响展开研究,提高公众接受度,并对植物性饮食的个性化干预进行更为系统、深入的研究。
基金supported by the National Natural Science Foundation of China,No.81072877Key Laboratory Project of Condition and Platform Construction Plan of Shenzhen Scientific Research Fund,No.CXB201111250113AShenzhen Scientific and Technology Development Program,No.201203149
文摘Mesenchymal stem cell transplantation is a novel means of treating cerebral ischemia/reper- fusion, and can promote angiogenesis and neurological functional recovery. Acupuncture at Conception and Governor vessels also has positive effects as a treatment for cerebral ischemia/ reperfusion. Therefore, we hypothesized that electro-acupuncture at Conception and Governor vessels plus mesenchymal stem cell transplantation may have better therapeutic effects on the promotion of angiogenesis and recovery of neurological function than either treatment alone. In the present study, human umbilical cord blood-derived mesenchymal stem cells were isolated, cultured, identified and intracranially transplanted into the striatum and subcortex of rats at 24 hours following cerebral ischemia/reperfusion. Subsequently, rats were electro-acupunctured at Conception and Governor vessels at 24 hours after transplantation. Modified neurological severity scores and immunohistochemistry findings revealed that the combined interventions of electro-acupuncture and mesenchymal stem cell transplantation clearly improved neurological impairment and up-regulated vascular endothelial growth factor expression around the isch- emic focus. The combined intervention provided a better outcome than mesenchymal stem cell transplantation alone. These findings demonstrate that electro-acupuncture at Conception and Governor vessels and mesenchymal stem cell transplantation have synergetic effects on promot- ing neurological function recovery and angiogenesis in rats after cerebral ischemia/reperfusion.
基金Supported by the National Natural Science Foundation of China(No.81072877)Shenzhen Municipal Science and Technology Research Development and Funds and Platform Construction Plan Key Laboratory Program(No.CXB201111250113A)Shenzhen Municipal Science and Technology Plan Project(No.201203149)
文摘OBJECTIVE: To investigate the combinatorial effects of conception and governor vessel electroacupuncture(EA) and human umbilical cord blood-derived mesenchymal stem cells(HUCB-MSCs) on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion. METHODS: With the HUCB-MSCs isolated, cultured and identified and the models of cerebral ischemia-reperfusion established, the HUCB-MSCs of passage three were intracranially transplanted and the EA at conception and governor vessels was applied. The pathomorphologic lesion by hematoxylin-eosin staining and the cellular apoptosis by terminal deoxynucleotidyl transferase-mediated nick-end labeling method around the ischemic focus were observed. RESULTS: The cultured adherent HUCB-MSCs exhibited a spindle shape and expressed MSC-specific markers, with the cell purity and proliferation rate significantly increasing after the primary passage. HE staining showed that there were no pathological changes observed in the sham surgery group. However, in the PBS transplantation group, degeneration and necrosis of a great number of nerve cells were seen. In both the HUCB-MSCs transplantation group and the HUCB-MSCs transplantation + EA group, reparative changes of the pathomorphism of the tissue were found. Both combination treatment and simple MSCs treatment were able to improve the pathomorphorlogic lesion following cerebral ischemia and reduce the abnormal TUNEL-positive numbers, with former better than latter. CONCLUSION: HUCB-MSCs improve pathological lesions and inhibit the cellular apoptosis around the cerebral ischemic area. EA at conception and governor vessels also improve pathological lesion and inhibit the cellular apoptosis in rats treated with HUCB-MSCs transplantation, which effects were superior to that of simple HUCB-MSCs transplantation.
基金partially supported by Guangdong Innovative and Entrepreneurial Research Team Program (No. 201301S0105240297)by 111 Project
文摘Approximately 30% of pregnancies after implantation end up in spontaneous abortions, and 50% of them are caused by chromosomal abnormalities. However, the spectrum of genomic copy number variants （CNVs） in products of conception （POC） and the underlying gene- dosage-sensitive mechanisms causing spontaneous abortions remain largely unknown. In this study, array comparative genornic hybridiza- tion （aCGH） analysis was performed as a salvage procedure for 128 POC culture failure （POC-CF） samples and as a supplemental procedure for 106 POC normal karyotype （POC-NK） samples. Chromosomal abnormalities were detected in 10% of POC-CF and pathogenic CNVs were detected in 3.9% of POC-CF and 5.7% of POC-NK samples. Compiled results from this study and relevant case series through a literature review demonstrated an abnormality detection rate （ADR） of 35% for chromosomal abnormalities in POC-CF samples, 3.7% for pathogenic CNVs in POC-CF samples, and 4.6% for pathogenic CNVs in POC-NK samples. Ingenuity Pathway Analysis （IPA） was performed on the genes from pathogenic CNVs found in POC samples. The denoted primary gene networks suggested that apoptosis and cell proliferation pathways are involved in miscarriage. In summary, a similar spectrum of cytogenomic abnormalities was observed in POC culture success and POC-CF samples. A threshold effect correlating the number of dosage-sensitive genes in a chromosome with the observed frequency of autosomai trisomy is proposed. A rationalized approach using firstly fluorescence in situ hybridization （FISH） testing with probes of chromosomes X/Y/ 18, 13/21, and 15/16/22 for common aneuploidies and polyploidies and secondly aCGH for other cytogenomic abnormalities is recommended for POC-CF samples.
