Holo-tomographic flow cytometry for label-free phenotyping of suspended acute myeloid leukemia blasts is demonstrated.A concave segmentation algorithm is applied to 3D refractive index tomograms to quantify NPM1-mutat...Holo-tomographic flow cytometry for label-free phenotyping of suspended acute myeloid leukemia blasts is demonstrated.A concave segmentation algorithm is applied to 3D refractive index tomograms to quantify NPM1-mutation-associated cup-like nuclear morphologies,with virtual reality visualization offering engaging immersion.The method enables population-level detection of statistically significant shifts in 3D cell morphology,originally correlating phenotype with genotype.展开更多
文摘Holo-tomographic flow cytometry for label-free phenotyping of suspended acute myeloid leukemia blasts is demonstrated.A concave segmentation algorithm is applied to 3D refractive index tomograms to quantify NPM1-mutation-associated cup-like nuclear morphologies,with virtual reality visualization offering engaging immersion.The method enables population-level detection of statistically significant shifts in 3D cell morphology,originally correlating phenotype with genotype.
