Multiple BAc FisH is a powerful tool for modern cytogenetic researching in both animals and plant s.But in cotton,this technique is unavailable due to the high percentage of repetitive sequences.Here,we identified twe...Multiple BAc FisH is a powerful tool for modern cytogenetic researching in both animals and plant s.But in cotton,this technique is unavailable due to the high percentage of repetitive sequences.Here,we identified twenty BACs from more than fifty BACs,and successfully demonstrated the use of multiple BAC-FISHfor cytogenetic re search in a diploid cotto n species,G.arboreum.The karyoty-ping should be a basic application of this technique,but the potential usage such as higlrresolution physical mapping construction,assisting BAC-by-BAC sequencing will be invaluable.展开更多
The phase sensitive NOESY spectrum of oridonin was treated using Full Relaxation Matrix Analysis(FRMA) approach, and the cross relaxation rates of proton pairs were obtained by diagonalizing the NOE matrix of oridonin...The phase sensitive NOESY spectrum of oridonin was treated using Full Relaxation Matrix Analysis(FRMA) approach, and the cross relaxation rates of proton pairs were obtained by diagonalizing the NOE matrix of oridonin. The inter proton distances were calculated according to 1/r6 ij ∝σij. The three-dimensional structure of oridonin in solution was calculated by the combination of WUPH, WUPH-S method with molecular mechanics minimization on the basis of NMR experiment.展开更多
文摘Multiple BAc FisH is a powerful tool for modern cytogenetic researching in both animals and plant s.But in cotton,this technique is unavailable due to the high percentage of repetitive sequences.Here,we identified twenty BACs from more than fifty BACs,and successfully demonstrated the use of multiple BAC-FISHfor cytogenetic re search in a diploid cotto n species,G.arboreum.The karyoty-ping should be a basic application of this technique,but the potential usage such as higlrresolution physical mapping construction,assisting BAC-by-BAC sequencing will be invaluable.
基金Supported by the National Natural Science Foundation of China
文摘The phase sensitive NOESY spectrum of oridonin was treated using Full Relaxation Matrix Analysis(FRMA) approach, and the cross relaxation rates of proton pairs were obtained by diagonalizing the NOE matrix of oridonin. The inter proton distances were calculated according to 1/r6 ij ∝σij. The three-dimensional structure of oridonin in solution was calculated by the combination of WUPH, WUPH-S method with molecular mechanics minimization on the basis of NMR experiment.
