Objective:To evaluate and compare coagulation and hematological parameters in hypertensive and normotensive pregnant women.Methods:This present cross-sectional study was carried out in the Departments of Pathology and...Objective:To evaluate and compare coagulation and hematological parameters in hypertensive and normotensive pregnant women.Methods:This present cross-sectional study was carried out in the Departments of Pathology and Obstetrics&Gynaecology at Dr.D.Y.Patil Medical College,Hospital&Research Centre,Pimpri,Pune,India from September 2023 to March 2025.Hematological parameters[platelet count,mean platelet volume(MPV),platelet distribution width(PDW)]were analyzed using an automated hematology analyzer,while coagulation parameters[prothrombin time(PT)/international normalised ratio,activated partial thromboplastin time(aPTT),and D-dimer]were assessed by standard automated assays.Results were compared between normotensive and hypertensive groups and correlated with disease severity.Results:The study included 212 antenatal females,with 106 normotensive pregnant women and 106 hypertensive women.Hypertensive women include cases of gestational hypertension(n=55);mild preeclampsia(n=39),and severe preeclampsia(n=12).A significant progressive decrease in platelet count and significant increases in MPV,PDW,PT,aPTT,and D-dimer levels were associated with increasing severity of pregnancy-induced hypertension(P<0.001).Women with severe preeclampsia had the lowest mean platelet counts and the highest coagulation parameter values compared to women with gestational hypertension,mild preeclampsia,and normotensive pregnancies.These findings indicate enhanced platelet activation,endothelial dysfunction,and activation of the coagulation–fibrinolytic system with worsening disease severity.Conclusions:Significant hematologic and coagulation abnormalities were present in women with pregnancy-induced hypertension.For better maternal-fetal outcomes and early management,routine monitoring is essential.展开更多
The essential step to improve the coagulation efficiency is to select and optimize the pre-oxidation process.Ultraviolet/sodium percarbonate(UV/SPC)can be employed as an alternative system to UV/H_(2)O_(2) in drinking...The essential step to improve the coagulation efficiency is to select and optimize the pre-oxidation process.Ultraviolet/sodium percarbonate(UV/SPC)can be employed as an alternative system to UV/H_(2)O_(2) in drinking water treatment plant operation,benefiting from the solid stability properties with the equivalent effect.At present,the studies on UV/SPC,and other UV/advanced oxidation processes(UV/H_(2)O_(2),UV/persulfate,UV/chlorine,etc.),mostly focus on the advanced water treatment after filtration.This study notes that UV/SPC has advantages in the pre-oxidized organics.UV/SPC improving the effect of coagulation on dissolved organic carbon(DOC)elimination by up to 25%,the optimal UV dose and SPC dosage are 600 mJ/cm^(2) and 20 mg/L verified by a pilot experiment;the decomposition degree and conversion of organics which is most conducive to follow-up coagulation treatment is obtained through the detailed comparison of the influence range of UV dose or the CSPC on the molecule structure of organics;the disparate role of UV was unraveled in UV photolysis pre-oxidation and UV/SPC pre-oxidation and coagulation,including the influence regularity of UV transmittance(UVT)and processing flow rate on UV dose;confirm that UV/SPC alleviates the risk of the generation of disinfection byproducts(DBPs)to 51.15%.This study corroborates the potential of UV/SPC as a pre-oxidation technology at the application level.展开更多
Snake venoms,especially those from the two subfamilies,Crotalinae and Viperinae,contained a lot of serine proteases. They were responsible for the hemorrhage,shock,or disorder of blood coagulation after envenomation. ...Snake venoms,especially those from the two subfamilies,Crotalinae and Viperinae,contained a lot of serine proteases. They were responsible for the hemorrhage,shock,or disorder of blood coagulation after envenomation. They acted,by activating,inactivating,or other converting effects,on almost all the components of hemostatic and fibrinolytic systems. Their sequences were homologous to trypsin-kallikrein serine proteases. Variation of primary sequences out of active center results in the difference of substrate specificities and the further difference of biological and pharmacological activities. Because of their common and unique properties compared to their physiological corresponding factors,snake venom proteases are proved to be an excellent model for the study of protease substrate discriminating mechanism. Furthermore,they have found an important position both in basic research and application of hemostasis and thrombosis in clinic.展开更多
BACKGROUND Gastric cancer(GC)is a prevalent malignancy with a substantial health burden and high mortality rate,despite advances in prevention,early detection,and treatment.Compared with the global average,Asia,notabl...BACKGROUND Gastric cancer(GC)is a prevalent malignancy with a substantial health burden and high mortality rate,despite advances in prevention,early detection,and treatment.Compared with the global average,Asia,notably China,reports disproportionately high GC incidences.The disease often progresses asymptoma-tically in the early stages,leading to delayed diagnosis and compromised out-comes.Thus,it is crucial to identify early diagnostic biomarkers and enhance treatment strategies to improve patient outcomes and reduce mortality.METHODS Retrospectively analyzed the clinical data of 148 patients with GC treated at the Civil Aviation Shanghai Hospital between December 2022 and December 2023.The associations of coagulation indices-partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT),fibrinogen,fibrinogen degradation products(FDP),fasting blood glucose,and D-dimer(D-D)with TNM stage and distant metastasis were examined.RESULTS Prolongation of APTT,PT,and TT was significantly correlated with the GC TNM stage.Hence,abnormal coagulation system activation was closely related to disease progression.Elevated FDP and D-D were significantly associated with distant metastasis in GC(P<0.05),suggesting that increased fibrinolytic activity contributes to increased metastatic risk.CONCLUSION Our Results reveal coagulation indices,FDPs as GC biomarkers,reflecting abnormal coagulation/fibrinolysis,aiding disease progression,metastasis prediction,and helping clinicians assess thrombotic risk for early intervention and personalized treatment plans.展开更多
Since December 2019,a novel type of coronavirus disease(COVID-19)in Wuhan led to an outbreak throughout China and the rest of the world.To date,there have been more than 1,260,000 COVID-19 patients,with a mortality ra...Since December 2019,a novel type of coronavirus disease(COVID-19)in Wuhan led to an outbreak throughout China and the rest of the world.To date,there have been more than 1,260,000 COVID-19 patients,with a mortality rate of approximately 5.44%.Studies have shown that coagulation dysfunction is a major cause of death in patients with severe COVID-19.Therefore,the People’s Liberation Army Professional Committee of Critical Care Medicine and Chinese Society on Thrombosis and Hemostasis grouped experts from the frontline of the Wuhan epidemic to come together and develop an expert consensus on diagnosis and treatment of coagulation dysfunction associated with a severe COVID-19 infection.This consensus includes an overview of COVID-19-related coagulation dysfunction,tests for coagulation,anticoagulation therapy,replacement therapy,supportive therapy and prevention.The consensus produced 18 recommendations which are being used to guide clinical work.展开更多
Coronavirus disease 2019(COVID-19)is,at present,one of the most relevant global health problems.In the literature hepatic alterations have been described in COVID-19 patients,and they are mainly represented by worseni...Coronavirus disease 2019(COVID-19)is,at present,one of the most relevant global health problems.In the literature hepatic alterations have been described in COVID-19 patients,and they are mainly represented by worsening of underlying chronic liver disease leading to hepatic decompensation and liver failure with higher mortality.Several potential mechanisms used by severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)to cause liver damage have been hypothesized.COVID-19 primary liver injury is less common than secondary liver injury.Most of the available data demonstrate how liver damage in SARSCo V-2 infection is likely due to systemic inflammation,and it is less likely mediated by a cytopathic effect directed on liver cells.Moreover,liver alterations could be caused by hypoxic injury and drugs(antibiotics and non-steroidal antiinflammatory drugs,remdesivir,tocilizumab,tofacitinib and dexamethasone).SARS-Co V-2 infection can induce multiple vascular district atherothrombosis by affecting simultaneously cerebral,coronary and peripheral vascular beds.Data in the literature highlight how the virus triggers an exaggerated immune response,which added to the cytopathic effect of the virus can induce endothelial damage and a prothrombotic dysregulation of hemostasis.This leads to a higher incidence of symptomatic and confirmed venous thrombosis and of pulmonary embolisms,especially in central,lobar or segmental pulmonary arteries,in COVID-19.There are currently fewer data for arterial thrombosis,while myocardial injury was identified in 7%-17%of patients hospitalized with SARS-Co V-2 infection and 22%-31%in the intensive care unit setting.Available data also revealed a higher occurrence of stroke and more serious forms of peripheral arterial disease in COVID-19 patients.Hemostasis dysregulation is observed during the COVID-19 course.Lower platelet count,mildly increased prothrombin time and increased Ddimer are typical laboratory features of patients with severe SARS-Co V-2 infection,described as“COVID-19 associated coagulopathy.”These alterations are correlated to poor outcomes.Moreover,patients with severe SARS-Co V-2 infection are characterized by high levels of von Willebrand factor with subsequent ADAMTS13 deficiency and impaired fibrinolysis.Platelet hyperreactivity,hypercoagulability and hypofibrinolysis during SARS-Co V-2 infection induce a pathological state named as“immuno-thromboinflammation.”Finally,liver dysfunction and coagulopathy are often observed at the same time in patients with COVID-19.The hypothesis that liver dysfunction could be mediated by microvascular thrombosis has been supported by postmortem findings and extensive vascular portal and sinusoidal thrombosis observation.Other evidence has shown a correlation between coagulation and liver damage in COVID-19,underlined by the transaminase association with coagulopathy,identified through laboratory markers such as prothrombin time,international normalized ratio,fibrinogen,D-dimer,fibrin/fibrinogen degradation products and platelet count.Other possible mechanisms like immunogenesis of COVID-19 damage or massive pericyte activation with consequent vessel wall fibrosis have been suggested.展开更多
BACKGROUND:This study aimed to explore the importance of routine coagulation tests for the early detection of sepsis and to quickly identify patients at a high risk of mortality.METHODS:This retrospective single-cente...BACKGROUND:This study aimed to explore the importance of routine coagulation tests for the early detection of sepsis and to quickly identify patients at a high risk of mortality.METHODS:This retrospective single-center study collected data from patients meeting the clinical criteria for systemic inflammatory response syndrome(SIRS) with a confirmed infection source.Patients with coagulation disorders or on medications affecting coagulation were excluded.Patients were divided into sepsis and non-sepsis groups based on a Sequential Organ Failure Assessment(SOFA) score of ≥2.Univariate and multivariate logistic regression identified indicators from routine coagulation tests that predict sepsis.Prognostic roles of coagulation indicators were analyzed within the sepsis group.RESULTS:A total of 512 patients were included,with 396 in the sepsis group and 116 in the non-sepsis group.The predictive factors in the sepsis prediction model encompass fibrin degradation products(FDP),D-dimer,lactate,procalcitonin(PCT) levels and the utilization of mechanical ventilation.Early elevation of FDP and D-dimer levels predicted sepsis onset.The model exhibited an area under the curve(AUC) of 0.943(95% CI:0.923–0.963).In the sepsis group,Cox regression analysis revealed an association between prothrombin time(PT) and in-hospital mortality.CONCLUSION:Abnormal high FDP and D-dimer levels in the early stages of sepsis provide a supplementary method for predicting sepsis.As the disease progresses,prolonged PT in the early stages of sepsis suggests a poor prognosis.展开更多
[Objectives] To study the effects of Xuebijing combined with ulinastatin on coagulation function of sepsis patients. [Methods]Fifty six patients conforming to the diagnosis criteria for sepsis and receiving the treatm...[Objectives] To study the effects of Xuebijing combined with ulinastatin on coagulation function of sepsis patients. [Methods]Fifty six patients conforming to the diagnosis criteria for sepsis and receiving the treatment from May 2015 to January 2019 were selected. They were randomly divided into treatment group and control group according to the order of treatment. The two groups were treated according to the International Guidelines for Management of Severe Sepsis and Septic Shock( 2012),in which ulinastatin and Xuebijing were added to the treatment group. Before the treatment and on the seventh day after the treatment,both groups of patients were measured for the activated partial thromboplastin time( APTT),fibrinogen( Fi B),platelet( PLT),and prothrombin time( PT) were measured;the treatment status of the both groups was continuously observed for 7 d,and the mechanical ventilation time,ICU hospitalization time,and 30-day survival rate were recorded. [Results] Both FIB and PLT of the treatment group were significantly increased,and both APTT and PT were significantly shortened.Compared with the control group,the difference was statistically significant( P < 0. 05). The mechanical ventilation time and ICU hospitalization time of the treatment group were significantly shorter than that of the control group,and the 30-day survival rate of the treatment group was significantly higher than that of the control group,and the difference was statistically significant( P < 0. 05). [Conclusions]Xuebijing combined with ulinastatin can improve coagulation disorders of sepsis patients.展开更多
BACKGROUND Rheumatoid arthritis(RA)is a common chronic inflammatory autoimmune disease with the main clinical feature of progressive joint synovial inflammation,which can lead to joint deformities as well as disabilit...BACKGROUND Rheumatoid arthritis(RA)is a common chronic inflammatory autoimmune disease with the main clinical feature of progressive joint synovial inflammation,which can lead to joint deformities as well as disability.RA often causes damage to multiple organs and systems within the body,including the blood hemostasis system.Few reports have focused on acquired coagulation dysfunction resulting from vitamin K-dependent coagulation factor deficiency associated with RA.CASE SUMMARY A 64-year-old woman with a history of RA presented to our hospital,complaining of painless gross hematuria for 2 wk.Blood coagulation function tests showed increased prothrombin time,international normalized ratio,and activated partial thromboplastin time.Abnormal blood coagulation factor(F)activity was detected(FII,7.0%;FV,122.0%;and FX,6.0%),indicating vitamin K-dependent coagulation factor deficiency.Thromboelastography and an activated partial thromboplastin time mixed correction experiment also suggested decreased coagulation factor activity.Clinically,the patient was initially diagnosed with hematuria,RA,and vitamin K-dependent coagulation factor deficiency.The patient received daily intravenous administration of vitamin K120 mg,etamsylate 3 g,and vitamin C 3000 mg for 10 d.Concurrently,oral leflunomide tablets and prednisone were administered for treatment of RA.After the treatment,the patient's symptoms improved markedly and she was discharged on day 12.There were no hemorrhagic events during 18 mo of follow-up.CONCLUSION RA can result in vitamin K-dependent coagulation factor deficiency,which leads to acquired coagulation dysfunction.Vitamin K1 supplementation has an obvious effect on coagulation dysfunction under these circumstances.展开更多
Objective: To investigate the effects of butyphthalide + alteplase (rt-PA) intravenous thrombolysis on the diffusion-weighted imaging (DWI) characteristics, coagulation function and neurological function in patients w...Objective: To investigate the effects of butyphthalide + alteplase (rt-PA) intravenous thrombolysis on the diffusion-weighted imaging (DWI) characteristics, coagulation function and neurological function in patients with acute cerebral infarction. Methods: The patients with acute cerebral infarction who were admitted to our hospital between April 2015 and October 2018 and with the onset time 4.5 hours were selected and divided into the observation group receiving butyphthalide + rt-PA intravenous thrombolysis and the control group receiving rt-PA intravenous thrombolysis by random number table. The differences in DWI parameter apparent diffusion coefficient (ADC), coagulation function indexes and neurological function indexes were compared between the two groups. Results: At 7 and 14 days after treatment, the ADC values of both groups were significantly increased, and the ADC values of the observation group were significantly higher than those of the control group;at 7 days after treatment, the prothrombin time (PT) and activated partial thromboplastin time (APTT) levels in both groups were significantly prolonged whereas fibrinogen (FIB), D-dimer (D-D), platelet activating factor (PAF), P-selectin, von Willebrand factor (vWF), neuron-specific enolase (NSE), S100B protein (S100B), malondialdehyde (MDA) and endothelin-1 (ET-1) contents were significantly decreased, and the APTT and PT levels in the observation group were significantly shorter than those in the control group whereas FIB, D-D, PAF, P-selectin, vWF, NSE, S100B, MDA and ET-1 contents were significantly lower than those in the control group. Conclusion: Butyphthalide + rt-PA intravenous thrombolysis can improve the DWI characteristics, coagulation function and neurological function of patients with acute cerebral infarction.展开更多
Objective:To investigate the effects of Ateprase combined with Butylphthalide on antioxidant capacity, coagulation function and related factors in patients with cerebral infarction.Methods:A total of 80 patients with ...Objective:To investigate the effects of Ateprase combined with Butylphthalide on antioxidant capacity, coagulation function and related factors in patients with cerebral infarction.Methods:A total of 80 patients with cerebral infarction who were treated in department of Neurology of our hospital from June 2015 to June 2018 were randomly divided into control group and observation group, with 40 cases in each group. Patients in the control group were treated with Butylphthalidel, while patients in the observation group were treated with Ateprase combined with Butylphthalide. The levels of superoxide dismutase (SOD), serum malondialdehyde (MDA), activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), fibrinogen (FIB), vascular endothelial growth factor (VEGF), nitric oxide (NO), endothelin (ET-1), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) were measured and compared before and after treatment.Results: After treatment: the levels of SOD, APTT, PT, TT, VEGF and NO in the serum of the control group and the observation group were significantly increased (P<0.05), and the levels of MDA, FIB, TNF- , CRP and ET-1 were significantly decreased (P<0.05);the trend of the above indicators was significantly higher than that of the control group (P<0.05).Conclusions: Ateplase combined with Butylphthalide in the treatment of cerebral infarction can significantly enhance the antioxidant capacity, improve blood coagulation and endothelial function, alleviate inflammation, and it has a good clinical effect.展开更多
Objective:To investigate the effect of ganglioside sodium on stress, neural injury degree, rebuilding related factors of neural function and coagulation index in patients with severe craniocerebral injury.Methods: Fro...Objective:To investigate the effect of ganglioside sodium on stress, neural injury degree, rebuilding related factors of neural function and coagulation index in patients with severe craniocerebral injury.Methods: From June 2016 to March 2018, 90 cases of severe craniocerebral injury in our hospital were randomly divided into 45 cases of ganglioside sodium group (group GM1) and 45 cases of control group. The levels of stress [including norepinephrine (NE) and cortisol (Cor)], nerve injury [including neuron-specific enolase (NSE), astrocyte-derived protein (S100beta), ubiquitin carboxyl terminal hydrolase L1 (UCH-L1), glial fibrillary acidic protein (GFAP)], nerve function reconstruction [including brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF)] and coagulation function [including prothrombin time (PT), thrombin time (TT), activated partial prothrombin time (APTT) and fibrinogen (FIB)] were observed and compared between the two groups. Results:Before treatment, there was no significant difference in stress hormone, nerve injury degree, nerve function reconstruction and coagulation function between the two groups.After treatment, the levels of cytokines and FIB in the two groups were significantly higher than those before treatment, and the levels of stress hormone, nerve injury molecule and TT were significantly lower than those before treatment.The levels of cytokines and FIB in GM1 group were significantly higher than those in the control group. The levels of stress hormone, nerve injury molecule and TT in GM1 group were significantly lower than those in the control group. There was no significant difference in the levels of PT and APTT between the two groups before and after treatment.Conclusions: The treatment of severe craniocerebral injury with ganglioside sodium on the basis of routine treatment can relieve body stress and nerve damage, also, facilitate nerve function reconstruction and improve coagulation function.展开更多
BACKGROUND Botulinum toxin(BoNT)is a bacterial poison that acts by blocking the release of acetylcholine-containing vesicles at the neuromuscular junction.Notably,a mild amount of BoNT is known to exert therapeutic be...BACKGROUND Botulinum toxin(BoNT)is a bacterial poison that acts by blocking the release of acetylcholine-containing vesicles at the neuromuscular junction.Notably,a mild amount of BoNT is known to exert therapeutic benefits against various diseases,including migraine,movement disorders,anxiety and neurocognitive deficits.BoNT treatment appears to increase platelet count in circulation.Therefore,BoNT treatment may be associated with the regulation of blood coagulation upon haemorrhagic events.However,the effects of BoNT on the degree of bleeding and clotting events have not yet been determined.AIM To investigate the effect of BoNT on the bleeding parameters and blood coagulation events in experimental mice.METHODS A group of 7-8-month-old mice was intramuscularly injected with a mild single dose of BoNT.After a month of BoNT injection,animals were subjected to tail bleeding assay,assessment of clotting time,and degree of platelet aggregation in comparison with the control group.RESULTS Results revealed that BoNT injection significantly reduced blood loss and bleeding time in experimental aging mice upon tail tip transection.Moreover,the blood samples collected from the BoNT-treated mice showed enhanced platelet aggregation and intense formation of the fibrin clot compared to the control.This study indicates a putative therapeutic value of BoNT in mitigating bleeding episodes,possibly through its platelet-enhancing property.CONCLUSION BoNT treatment effectively facilitates blood coagulation.Upon further validation,this approach can be translated to treat traumatic blood vessel injuries,haemorrhagic diseases,and bleeding complications associated with surgical procedures.展开更多
Objective:To explore the changes of coagulation function, cytokines and T lymphocyte in patients undergoing laparoscopic radical gastrectomy for gastric cancer and its clinical significance.Methods: 40 cases of laparo...Objective:To explore the changes of coagulation function, cytokines and T lymphocyte in patients undergoing laparoscopic radical gastrectomy for gastric cancer and its clinical significance.Methods: 40 cases of laparoscopic radical gastrectomy for gastric cancer patients and 40 cases of open radical gastrectomy for gastric cancer patients in our hospital were selected to detect and investigate the perioperative coagulation function [APTT (activated partial thromboplastin time), FIB (fibrinogen), and PLT (platelet)], cytokines [CRP (C reactive protein), IL-6 (IL-6) and TNF-alpha (Tumor necrosis factor-alpha)] and T lymphocytes (CD4+, CD8+ and CD4+/CD8+) changes and clinical meaning of patients in the two groups.Results: The coagulation function related indicators, cytokines and T lymphocytes of the two groups before treatment did not change significantly (P>0.05). 1 d after operation, blood coagulation, TNF-alpha, CD4+ and CD4+/CD8+ levels were significantly lower than that before operation in two groups of patients (P<0.05), while IL-6, CRP and CD8+ were significantly higher than that before the operation (P<0.05), and the index change in open group was more obvious. 3 d after surgery, the APTT, IL-6, CRP, CD4+, CD8+ and PLT levels in two group patients were significantly lower than that 1 d after surgery, while FIB, TNF-alpha and CD4+/CD8+ were significantly higher than that 1 d after surgery;blood coagulation index, TNF-alpha and CD4+ and CD4+/CD8+ were significantly lower in the laparotomy group patients than in laparoscopic group, while IL-6, CRP and CD8+ were significantly higher than the laparoscopic group (P<0.05). 5 d after operation, the APTT, TNF-alpha, FIB, CD4+, CD4+/CD8+ and PLT in two groups were significantly higher than that 3 d after surgery (P<0.05), while IL-6, CRP and CD8+ levels were significantly lower than that of 3 d after surgery (P<0.05);blood coagulation index, TNF-alpha and CD4+ and CD4+/CD8+ in the laparotomy group patients were significantly lower than laparoscopic group (P<0.05), while IL-6, CRP and CD8+ were significantly higher than the laparoscopic group (P<0.05).Conclusions: Radical resection of gastric cancer can cause coagulation disorder, inhibit the immune function and inflammatory reaction, and gradually recover with the passage of time;Compared with open surgery, laparoscopic radical gastrectomy have smaller impact on patients and recovery faster.展开更多
As part of sewage treatment,coagulation could remove phosphorus from the effluent of the Anaerobic-Anoxic-Oxic-Anoxic(A^(2)OA)biological process.The importance in investigating the influence of coagulation on Anaerobi...As part of sewage treatment,coagulation could remove phosphorus from the effluent of the Anaerobic-Anoxic-Oxic-Anoxic(A^(2)OA)biological process.The importance in investigating the influence of coagulation on Anaerobic-Anoxic-Oxic-Anoxic Membrane Bioreactor(A^(2)OA-MBR)should be emphasized.In this study,systematic optimization of coagulation parameters for greater pollutant removal was conducted in terms of coagulant dosage,coagulation residence time and stirring hydraulic conditions.Coagulation process could remarkably remove turbidity,phosphorus,chemical oxygen demand,humic-like,protein-like and polysaccharide-like substances from secondary effluent and A^(2)OA sludge-liquid mixture.Furthermore,the influence of coagulation on membrane fouling development during the ultrafiltration of secondary effluent and A^(2)OA sludge-liquid mixture was investigated based on optimum coagulation parameters.Coagulation simultaneously reduced reversible membrane fouling and the irreversible one by 86%and 16%,respectively.According to excitation-emission matrix and attenuated total reflection-fourier transform infrared spectra,membrane fouling was primarily influenced by the cake layer,although pore fouling might be aggravated by A^(2)OA processes.Besides,the feasibility of coagulation-assisted A^(2)OA-MBRwas also assessed using hollowfibermembranes.It exhibited excellent potential in alleviatingmembrane fouling,while regular cleaning twice a day was not enough to suppress transmembrane pressure increase during direct domestic wastewater ultrafiltration.Additionally,both polysaccharide-like and protein-like foulants were vital components for membrane fouling during wastewater treatment.展开更多
BACKGROUND Coagulation status is closely related to the progression of malignant tumors.In the era of neoadjuvant immunochemotherapy(NICT),the prognostic utility of coagulation indicators in patients with locally adva...BACKGROUND Coagulation status is closely related to the progression of malignant tumors.In the era of neoadjuvant immunochemotherapy(NICT),the prognostic utility of coagulation indicators in patients with locally advanced gastric cancer(LAGC)undergoing new treatments remains to be determined.AIM To determine whether hypercoagulation is an effective prognostic indicator in patients with LAGC who underwent radical resection after NICT.METHODS A retrospective analysis of clinical data from 104 patients with LAGC,who underwent radical resection after NICT between 2020 and 2023,was performed.Ddimer and fibrinogen concentrations were measured one week before NICT,and again one week before surgery,to analyze the association between these two indicators and their combined indices[non-hypercoagulation(D-dimer and fibrinogen concentrations within the upper limit of normal)vs hypercoagulation(D-dimer or fibrinogen concentrations above the upper limit of normal)]with prognosis.After radical resection,patients were followed-up periodically.The median follow-up duration was 21 months.RESULTS Data collected after NICT revealed that the three-year overall survival(OS)and disease-free survival(DFS)rates the non-hypercoagulation group were significantly better than those in the hypercoagulation group[94.4%vs 78.0%(P=0.019)and 87.0%vs 68.0%(P=0.027),respectively].Multivariate analysis indicated that hypercoagulation after NICT was an independent factor for poor postoperative OS[hazard ratio(HR)4.436,P=0.023]and DFS(HR 2.551,P=0.039).Pre-NICT data demonstrated no statistically significant difference in three-year OS between the non-hypercoagulation and hypercoagulation groups(88.3%vs 84.1%,respectively;P=0.443).CONCLUSION Hypercoagulation after NICT is an effective prognostic indicator in patients with LAGC undergoing radical gastrectomy.展开更多
BACKGROUND Currently,there is a notable lack of reliable studies evaluating the impact of multidisciplinary treatment strategies following transarterial chemoembolization(TACE)on patients with hepatocellular carcinoma...BACKGROUND Currently,there is a notable lack of reliable studies evaluating the impact of multidisciplinary treatment strategies following transarterial chemoembolization(TACE)on patients with hepatocellular carcinoma(HCC),underscoring the urgent need for higher-level research in this area.AIM To investigate the association of multidisciplinary treatment strategies with the immunological,coagulation,and tumor biomarker responses after post-TACE in HCC.METHODS This retrospective analysis included 100 patients with HCC who were categorized based on the treatment approach into the control(patients treated with TACE alone)and experimental groups(patients receiving multidisciplinary treatment strategies post-TACE).Participant characteristics,short-term efficacy,and safety assessment as well as immunological,coagulation,and tumor biomarker res-ponses between the two groups were collected and compared.RESULTS Compared with the control group,the experimental group demonstrated a superior overall response rate,along with an increased fibrinogen,markedly improved immunological biomarker,lower prothrombin time,thrombin time,alpha-fetoprotein,carcinoembryonic antigen,and carbohydrate antigen 199 levels,as well as a decreased abnormal prothrombin incidence,and a lower overall rate of adverse reactions.Notably,no significant difference in the activated partial thromboplastin time and D-dimer levels was observed between the two groups.CONCLUSION Multidisciplinary treatment strategies post-TACE have improved the treatment outcome,the immunological response,and the coagulation function,lowered the tumor biomarker response levels,and reduced the risk of adverse reactions in patients with HCC.展开更多
Gastric cancer(GC)has remained one of the leading causes of cancer-related deaths globally.The development of noninvasive biomarkers in cancer diagnosis and treatment has gained substantial traction in recent years.Re...Gastric cancer(GC)has remained one of the leading causes of cancer-related deaths globally.The development of noninvasive biomarkers in cancer diagnosis and treatment has gained substantial traction in recent years.Recent evidence highlights hypercoagulation as a promising prognostic biomarker,particularly in locally advanced GC(LAGC)who underwent radical resection after neoadjuvant immunochemotherapy(NICT).A recent study by Li et al showed that hypercoagulation is a valuable prognostic indicator for patients with LAGC who have undergone radical resection following NICT.While the study addresses an important clinical issue and provides insightful findings,the present study offered valuable insights;the applicability of these findings was constrained by the retrospective design,the focus on a single center,and the small sample size of the existing studies.Additionally,vital confounders,such as preoperative comorbidities and systemic inflammation,are inadequately addressed.Future studies should focus on prospective multicenter trials,incorporating advanced predictive models such as machine learning algorithms to integrate coagulation markers with other clinical variables for personalized risk stratification.In addition,we are required to validate findings to examine the biological mechanisms correlating hypercoagulation to tumor progression.Integrating machine learning,comprehensive biomarker panels,and real-world data would allow the researchers to have personalized risk stratification,improve predictive accuracy,and optimize clinical decision-making.Finally,A multidisciplinary approach,including lifestyle interventions and imaging modalities,is essential to improve outcomes among patients with GC.展开更多
Objective:To investigate the effects of intravenous thrombolysis therapy with alteplase on neurological function,coagulation function and serum inflammatory factors in patients with acute cerebral infarction.Methods:A...Objective:To investigate the effects of intravenous thrombolysis therapy with alteplase on neurological function,coagulation function and serum inflammatory factors in patients with acute cerebral infarction.Methods:A total of 96 patients with acute cerebral infarction admitted to our hospital from September 2017 to October 2019 were randomly divided into two groups,with 48 patients in each group.The control group(n=48)received routine treatment,and the observation group received intravenous thrombolysis therapy with alteplase on the basis of routine treatment.The neurological deficit score,prothrombin time(PT),activated partial thromboplastin time(APTT),tumor necrosis factor-a level(TNF-α),and high-sensitivity C-reactive protein(hs-CRP)were compared between the two groups after 15 days of treatment.Results:After treatment,NIHSS scores in both groups were lower than those before treatment;PT levels were increased,while APTT,TNF-αand hs-CRP levels were all decreased in both groups,and the changes in the observation group were greater than those in the control group,with statistically significant difference(P<0.05).Conclusions:Intravenous thrombolysis therapy with alteplase can improve the neurological function,coagulation function and serum levels of inflammatory factors in patients with acute cerebral infarction,which is worthy of clinical application.展开更多
BACKGROUND Colon polyps represent a significant clinical challenge in elderly patients.While endoscopic mucosal resection(EMR)and argon plasma coagulation(APC)are widely used,their comparative effectiveness in elderly...BACKGROUND Colon polyps represent a significant clinical challenge in elderly patients.While endoscopic mucosal resection(EMR)and argon plasma coagulation(APC)are widely used,their comparative effectiveness in elderly populations remains unclear,particularly regarding postoperative recovery and complication profiles.AIM To compare the postoperative recovery,complications and efficacy of EMR and APC in elderly patients with colonic polyps.METHODS We retrospectively analyzed clinical data from 224 elderly patients with colon polyps treated at our center between January 2021 and July 2024.All patients were divided into the EMR group and APC group according to the surgical method they received.By comparing the operation time,intraoperative bleeding situation,hospital stays,postoperative inflammatory response index,complication rate and recurrence status of the two groups,the effect of the two surgical methods was comprehensively evaluated.RESULTS The APC group exhibited superior outcomes in terms of operative time(10.63 minutes vs 13.27 minutes,P<0.001),intraoperative bleeding situation(39.00%vs 52.42%,P=0.031),and length of hospital stay(1.63 days vs 3.87 days,P<0.001)compared to the EMR group.The one-time resection rate of the APC group(94.69%)was higher than that of the EMR group(89.14%)(P=0.026).The overall effective rates of the two groups were 94.35%and 92.00%,respectively.Postoperative procalcitonin and C-reactive protein levels were lower in the APC group than in the EMR group(P<0.001).The incidence of complications was comparable between the two groups(P=0.159).The recurrence rate was lower in the APC group(2.00%)than in the EMR group(8.06%)(P=0.045).CONCLUSION For elderly patients with colon polyps,APC showed certain advantages compared with EMR in promoting postoperative recovery,reducing the inflammatory response and the risk of complications.However,the study is limited by its single-center retrospective design and short follow-up period,and further multicenter prospective studies are needed to validate the findings.展开更多
文摘Objective:To evaluate and compare coagulation and hematological parameters in hypertensive and normotensive pregnant women.Methods:This present cross-sectional study was carried out in the Departments of Pathology and Obstetrics&Gynaecology at Dr.D.Y.Patil Medical College,Hospital&Research Centre,Pimpri,Pune,India from September 2023 to March 2025.Hematological parameters[platelet count,mean platelet volume(MPV),platelet distribution width(PDW)]were analyzed using an automated hematology analyzer,while coagulation parameters[prothrombin time(PT)/international normalised ratio,activated partial thromboplastin time(aPTT),and D-dimer]were assessed by standard automated assays.Results were compared between normotensive and hypertensive groups and correlated with disease severity.Results:The study included 212 antenatal females,with 106 normotensive pregnant women and 106 hypertensive women.Hypertensive women include cases of gestational hypertension(n=55);mild preeclampsia(n=39),and severe preeclampsia(n=12).A significant progressive decrease in platelet count and significant increases in MPV,PDW,PT,aPTT,and D-dimer levels were associated with increasing severity of pregnancy-induced hypertension(P<0.001).Women with severe preeclampsia had the lowest mean platelet counts and the highest coagulation parameter values compared to women with gestational hypertension,mild preeclampsia,and normotensive pregnancies.These findings indicate enhanced platelet activation,endothelial dysfunction,and activation of the coagulation–fibrinolytic system with worsening disease severity.Conclusions:Significant hematologic and coagulation abnormalities were present in women with pregnancy-induced hypertension.For better maternal-fetal outcomes and early management,routine monitoring is essential.
基金supported by the Integrated Management of Water Resources and Water Environment in the Yangtze River,Yellow River and Other Key Basins(No.2021YFC3201304)Beijing Water Group Research Institute(China)for its support.
文摘The essential step to improve the coagulation efficiency is to select and optimize the pre-oxidation process.Ultraviolet/sodium percarbonate(UV/SPC)can be employed as an alternative system to UV/H_(2)O_(2) in drinking water treatment plant operation,benefiting from the solid stability properties with the equivalent effect.At present,the studies on UV/SPC,and other UV/advanced oxidation processes(UV/H_(2)O_(2),UV/persulfate,UV/chlorine,etc.),mostly focus on the advanced water treatment after filtration.This study notes that UV/SPC has advantages in the pre-oxidized organics.UV/SPC improving the effect of coagulation on dissolved organic carbon(DOC)elimination by up to 25%,the optimal UV dose and SPC dosage are 600 mJ/cm^(2) and 20 mg/L verified by a pilot experiment;the decomposition degree and conversion of organics which is most conducive to follow-up coagulation treatment is obtained through the detailed comparison of the influence range of UV dose or the CSPC on the molecule structure of organics;the disparate role of UV was unraveled in UV photolysis pre-oxidation and UV/SPC pre-oxidation and coagulation,including the influence regularity of UV transmittance(UVT)and processing flow rate on UV dose;confirm that UV/SPC alleviates the risk of the generation of disinfection byproducts(DBPs)to 51.15%.This study corroborates the potential of UV/SPC as a pre-oxidation technology at the application level.
文摘Snake venoms,especially those from the two subfamilies,Crotalinae and Viperinae,contained a lot of serine proteases. They were responsible for the hemorrhage,shock,or disorder of blood coagulation after envenomation. They acted,by activating,inactivating,or other converting effects,on almost all the components of hemostatic and fibrinolytic systems. Their sequences were homologous to trypsin-kallikrein serine proteases. Variation of primary sequences out of active center results in the difference of substrate specificities and the further difference of biological and pharmacological activities. Because of their common and unique properties compared to their physiological corresponding factors,snake venom proteases are proved to be an excellent model for the study of protease substrate discriminating mechanism. Furthermore,they have found an important position both in basic research and application of hemostasis and thrombosis in clinic.
文摘BACKGROUND Gastric cancer(GC)is a prevalent malignancy with a substantial health burden and high mortality rate,despite advances in prevention,early detection,and treatment.Compared with the global average,Asia,notably China,reports disproportionately high GC incidences.The disease often progresses asymptoma-tically in the early stages,leading to delayed diagnosis and compromised out-comes.Thus,it is crucial to identify early diagnostic biomarkers and enhance treatment strategies to improve patient outcomes and reduce mortality.METHODS Retrospectively analyzed the clinical data of 148 patients with GC treated at the Civil Aviation Shanghai Hospital between December 2022 and December 2023.The associations of coagulation indices-partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT),fibrinogen,fibrinogen degradation products(FDP),fasting blood glucose,and D-dimer(D-D)with TNM stage and distant metastasis were examined.RESULTS Prolongation of APTT,PT,and TT was significantly correlated with the GC TNM stage.Hence,abnormal coagulation system activation was closely related to disease progression.Elevated FDP and D-D were significantly associated with distant metastasis in GC(P<0.05),suggesting that increased fibrinolytic activity contributes to increased metastatic risk.CONCLUSION Our Results reveal coagulation indices,FDPs as GC biomarkers,reflecting abnormal coagulation/fibrinolysis,aiding disease progression,metastasis prediction,and helping clinicians assess thrombotic risk for early intervention and personalized treatment plans.
文摘Since December 2019,a novel type of coronavirus disease(COVID-19)in Wuhan led to an outbreak throughout China and the rest of the world.To date,there have been more than 1,260,000 COVID-19 patients,with a mortality rate of approximately 5.44%.Studies have shown that coagulation dysfunction is a major cause of death in patients with severe COVID-19.Therefore,the People’s Liberation Army Professional Committee of Critical Care Medicine and Chinese Society on Thrombosis and Hemostasis grouped experts from the frontline of the Wuhan epidemic to come together and develop an expert consensus on diagnosis and treatment of coagulation dysfunction associated with a severe COVID-19 infection.This consensus includes an overview of COVID-19-related coagulation dysfunction,tests for coagulation,anticoagulation therapy,replacement therapy,supportive therapy and prevention.The consensus produced 18 recommendations which are being used to guide clinical work.
文摘Coronavirus disease 2019(COVID-19)is,at present,one of the most relevant global health problems.In the literature hepatic alterations have been described in COVID-19 patients,and they are mainly represented by worsening of underlying chronic liver disease leading to hepatic decompensation and liver failure with higher mortality.Several potential mechanisms used by severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)to cause liver damage have been hypothesized.COVID-19 primary liver injury is less common than secondary liver injury.Most of the available data demonstrate how liver damage in SARSCo V-2 infection is likely due to systemic inflammation,and it is less likely mediated by a cytopathic effect directed on liver cells.Moreover,liver alterations could be caused by hypoxic injury and drugs(antibiotics and non-steroidal antiinflammatory drugs,remdesivir,tocilizumab,tofacitinib and dexamethasone).SARS-Co V-2 infection can induce multiple vascular district atherothrombosis by affecting simultaneously cerebral,coronary and peripheral vascular beds.Data in the literature highlight how the virus triggers an exaggerated immune response,which added to the cytopathic effect of the virus can induce endothelial damage and a prothrombotic dysregulation of hemostasis.This leads to a higher incidence of symptomatic and confirmed venous thrombosis and of pulmonary embolisms,especially in central,lobar or segmental pulmonary arteries,in COVID-19.There are currently fewer data for arterial thrombosis,while myocardial injury was identified in 7%-17%of patients hospitalized with SARS-Co V-2 infection and 22%-31%in the intensive care unit setting.Available data also revealed a higher occurrence of stroke and more serious forms of peripheral arterial disease in COVID-19 patients.Hemostasis dysregulation is observed during the COVID-19 course.Lower platelet count,mildly increased prothrombin time and increased Ddimer are typical laboratory features of patients with severe SARS-Co V-2 infection,described as“COVID-19 associated coagulopathy.”These alterations are correlated to poor outcomes.Moreover,patients with severe SARS-Co V-2 infection are characterized by high levels of von Willebrand factor with subsequent ADAMTS13 deficiency and impaired fibrinolysis.Platelet hyperreactivity,hypercoagulability and hypofibrinolysis during SARS-Co V-2 infection induce a pathological state named as“immuno-thromboinflammation.”Finally,liver dysfunction and coagulopathy are often observed at the same time in patients with COVID-19.The hypothesis that liver dysfunction could be mediated by microvascular thrombosis has been supported by postmortem findings and extensive vascular portal and sinusoidal thrombosis observation.Other evidence has shown a correlation between coagulation and liver damage in COVID-19,underlined by the transaminase association with coagulopathy,identified through laboratory markers such as prothrombin time,international normalized ratio,fibrinogen,D-dimer,fibrin/fibrinogen degradation products and platelet count.Other possible mechanisms like immunogenesis of COVID-19 damage or massive pericyte activation with consequent vessel wall fibrosis have been suggested.
基金supported by the Science and Technology Commission of Shanghai Municipality (grant no.22Y11900300)China Primary Health Care Foundation (grant no.MTP2022A0132)Project of Shanghai Administration of Traditional Chinese Medicine (ZXXT-202213)to EQM and LM。
文摘BACKGROUND:This study aimed to explore the importance of routine coagulation tests for the early detection of sepsis and to quickly identify patients at a high risk of mortality.METHODS:This retrospective single-center study collected data from patients meeting the clinical criteria for systemic inflammatory response syndrome(SIRS) with a confirmed infection source.Patients with coagulation disorders or on medications affecting coagulation were excluded.Patients were divided into sepsis and non-sepsis groups based on a Sequential Organ Failure Assessment(SOFA) score of ≥2.Univariate and multivariate logistic regression identified indicators from routine coagulation tests that predict sepsis.Prognostic roles of coagulation indicators were analyzed within the sepsis group.RESULTS:A total of 512 patients were included,with 396 in the sepsis group and 116 in the non-sepsis group.The predictive factors in the sepsis prediction model encompass fibrin degradation products(FDP),D-dimer,lactate,procalcitonin(PCT) levels and the utilization of mechanical ventilation.Early elevation of FDP and D-dimer levels predicted sepsis onset.The model exhibited an area under the curve(AUC) of 0.943(95% CI:0.923–0.963).In the sepsis group,Cox regression analysis revealed an association between prothrombin time(PT) and in-hospital mortality.CONCLUSION:Abnormal high FDP and D-dimer levels in the early stages of sepsis provide a supplementary method for predicting sepsis.As the disease progresses,prolonged PT in the early stages of sepsis suggests a poor prognosis.
文摘[Objectives] To study the effects of Xuebijing combined with ulinastatin on coagulation function of sepsis patients. [Methods]Fifty six patients conforming to the diagnosis criteria for sepsis and receiving the treatment from May 2015 to January 2019 were selected. They were randomly divided into treatment group and control group according to the order of treatment. The two groups were treated according to the International Guidelines for Management of Severe Sepsis and Septic Shock( 2012),in which ulinastatin and Xuebijing were added to the treatment group. Before the treatment and on the seventh day after the treatment,both groups of patients were measured for the activated partial thromboplastin time( APTT),fibrinogen( Fi B),platelet( PLT),and prothrombin time( PT) were measured;the treatment status of the both groups was continuously observed for 7 d,and the mechanical ventilation time,ICU hospitalization time,and 30-day survival rate were recorded. [Results] Both FIB and PLT of the treatment group were significantly increased,and both APTT and PT were significantly shortened.Compared with the control group,the difference was statistically significant( P < 0. 05). The mechanical ventilation time and ICU hospitalization time of the treatment group were significantly shorter than that of the control group,and the 30-day survival rate of the treatment group was significantly higher than that of the control group,and the difference was statistically significant( P < 0. 05). [Conclusions]Xuebijing combined with ulinastatin can improve coagulation disorders of sepsis patients.
基金the National Natural Science Foundation of China,No.81803917 and 81904024.
文摘BACKGROUND Rheumatoid arthritis(RA)is a common chronic inflammatory autoimmune disease with the main clinical feature of progressive joint synovial inflammation,which can lead to joint deformities as well as disability.RA often causes damage to multiple organs and systems within the body,including the blood hemostasis system.Few reports have focused on acquired coagulation dysfunction resulting from vitamin K-dependent coagulation factor deficiency associated with RA.CASE SUMMARY A 64-year-old woman with a history of RA presented to our hospital,complaining of painless gross hematuria for 2 wk.Blood coagulation function tests showed increased prothrombin time,international normalized ratio,and activated partial thromboplastin time.Abnormal blood coagulation factor(F)activity was detected(FII,7.0%;FV,122.0%;and FX,6.0%),indicating vitamin K-dependent coagulation factor deficiency.Thromboelastography and an activated partial thromboplastin time mixed correction experiment also suggested decreased coagulation factor activity.Clinically,the patient was initially diagnosed with hematuria,RA,and vitamin K-dependent coagulation factor deficiency.The patient received daily intravenous administration of vitamin K120 mg,etamsylate 3 g,and vitamin C 3000 mg for 10 d.Concurrently,oral leflunomide tablets and prednisone were administered for treatment of RA.After the treatment,the patient's symptoms improved markedly and she was discharged on day 12.There were no hemorrhagic events during 18 mo of follow-up.CONCLUSION RA can result in vitamin K-dependent coagulation factor deficiency,which leads to acquired coagulation dysfunction.Vitamin K1 supplementation has an obvious effect on coagulation dysfunction under these circumstances.
基金Major Project of Shanghai Science and Technology Commission (Sub-project) No: 11411950300
文摘Objective: To investigate the effects of butyphthalide + alteplase (rt-PA) intravenous thrombolysis on the diffusion-weighted imaging (DWI) characteristics, coagulation function and neurological function in patients with acute cerebral infarction. Methods: The patients with acute cerebral infarction who were admitted to our hospital between April 2015 and October 2018 and with the onset time 4.5 hours were selected and divided into the observation group receiving butyphthalide + rt-PA intravenous thrombolysis and the control group receiving rt-PA intravenous thrombolysis by random number table. The differences in DWI parameter apparent diffusion coefficient (ADC), coagulation function indexes and neurological function indexes were compared between the two groups. Results: At 7 and 14 days after treatment, the ADC values of both groups were significantly increased, and the ADC values of the observation group were significantly higher than those of the control group;at 7 days after treatment, the prothrombin time (PT) and activated partial thromboplastin time (APTT) levels in both groups were significantly prolonged whereas fibrinogen (FIB), D-dimer (D-D), platelet activating factor (PAF), P-selectin, von Willebrand factor (vWF), neuron-specific enolase (NSE), S100B protein (S100B), malondialdehyde (MDA) and endothelin-1 (ET-1) contents were significantly decreased, and the APTT and PT levels in the observation group were significantly shorter than those in the control group whereas FIB, D-D, PAF, P-selectin, vWF, NSE, S100B, MDA and ET-1 contents were significantly lower than those in the control group. Conclusion: Butyphthalide + rt-PA intravenous thrombolysis can improve the DWI characteristics, coagulation function and neurological function of patients with acute cerebral infarction.
文摘Objective:To investigate the effects of Ateprase combined with Butylphthalide on antioxidant capacity, coagulation function and related factors in patients with cerebral infarction.Methods:A total of 80 patients with cerebral infarction who were treated in department of Neurology of our hospital from June 2015 to June 2018 were randomly divided into control group and observation group, with 40 cases in each group. Patients in the control group were treated with Butylphthalidel, while patients in the observation group were treated with Ateprase combined with Butylphthalide. The levels of superoxide dismutase (SOD), serum malondialdehyde (MDA), activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), fibrinogen (FIB), vascular endothelial growth factor (VEGF), nitric oxide (NO), endothelin (ET-1), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) were measured and compared before and after treatment.Results: After treatment: the levels of SOD, APTT, PT, TT, VEGF and NO in the serum of the control group and the observation group were significantly increased (P<0.05), and the levels of MDA, FIB, TNF- , CRP and ET-1 were significantly decreased (P<0.05);the trend of the above indicators was significantly higher than that of the control group (P<0.05).Conclusions: Ateplase combined with Butylphthalide in the treatment of cerebral infarction can significantly enhance the antioxidant capacity, improve blood coagulation and endothelial function, alleviate inflammation, and it has a good clinical effect.
文摘Objective:To investigate the effect of ganglioside sodium on stress, neural injury degree, rebuilding related factors of neural function and coagulation index in patients with severe craniocerebral injury.Methods: From June 2016 to March 2018, 90 cases of severe craniocerebral injury in our hospital were randomly divided into 45 cases of ganglioside sodium group (group GM1) and 45 cases of control group. The levels of stress [including norepinephrine (NE) and cortisol (Cor)], nerve injury [including neuron-specific enolase (NSE), astrocyte-derived protein (S100beta), ubiquitin carboxyl terminal hydrolase L1 (UCH-L1), glial fibrillary acidic protein (GFAP)], nerve function reconstruction [including brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF)] and coagulation function [including prothrombin time (PT), thrombin time (TT), activated partial prothrombin time (APTT) and fibrinogen (FIB)] were observed and compared between the two groups. Results:Before treatment, there was no significant difference in stress hormone, nerve injury degree, nerve function reconstruction and coagulation function between the two groups.After treatment, the levels of cytokines and FIB in the two groups were significantly higher than those before treatment, and the levels of stress hormone, nerve injury molecule and TT were significantly lower than those before treatment.The levels of cytokines and FIB in GM1 group were significantly higher than those in the control group. The levels of stress hormone, nerve injury molecule and TT in GM1 group were significantly lower than those in the control group. There was no significant difference in the levels of PT and APTT between the two groups before and after treatment.Conclusions: The treatment of severe craniocerebral injury with ganglioside sodium on the basis of routine treatment can relieve body stress and nerve damage, also, facilitate nerve function reconstruction and improve coagulation function.
基金Supported by The Science and Engineering Research Board,No.SERB-EEQ/2016/000639The University Grants Commission-Faculty Recharge Programme(UGC-FRP),No.F4-5(97)/2014+2 种基金Rashtriya Uchchatar Shiksha Abhiyan(RUSA)2.0,Biological Sciences,Bharathidasan University,No.TN RUSA:311/RUSA(2.0)/2018Infrastructure of the Department of Animal Science,Bharathidasan University,UGC-SAP-DRS and DST-FISTand CSIR-SRF Direct,No.09/0475(23353)/2025-EMR-I.
文摘BACKGROUND Botulinum toxin(BoNT)is a bacterial poison that acts by blocking the release of acetylcholine-containing vesicles at the neuromuscular junction.Notably,a mild amount of BoNT is known to exert therapeutic benefits against various diseases,including migraine,movement disorders,anxiety and neurocognitive deficits.BoNT treatment appears to increase platelet count in circulation.Therefore,BoNT treatment may be associated with the regulation of blood coagulation upon haemorrhagic events.However,the effects of BoNT on the degree of bleeding and clotting events have not yet been determined.AIM To investigate the effect of BoNT on the bleeding parameters and blood coagulation events in experimental mice.METHODS A group of 7-8-month-old mice was intramuscularly injected with a mild single dose of BoNT.After a month of BoNT injection,animals were subjected to tail bleeding assay,assessment of clotting time,and degree of platelet aggregation in comparison with the control group.RESULTS Results revealed that BoNT injection significantly reduced blood loss and bleeding time in experimental aging mice upon tail tip transection.Moreover,the blood samples collected from the BoNT-treated mice showed enhanced platelet aggregation and intense formation of the fibrin clot compared to the control.This study indicates a putative therapeutic value of BoNT in mitigating bleeding episodes,possibly through its platelet-enhancing property.CONCLUSION BoNT treatment effectively facilitates blood coagulation.Upon further validation,this approach can be translated to treat traumatic blood vessel injuries,haemorrhagic diseases,and bleeding complications associated with surgical procedures.
文摘Objective:To explore the changes of coagulation function, cytokines and T lymphocyte in patients undergoing laparoscopic radical gastrectomy for gastric cancer and its clinical significance.Methods: 40 cases of laparoscopic radical gastrectomy for gastric cancer patients and 40 cases of open radical gastrectomy for gastric cancer patients in our hospital were selected to detect and investigate the perioperative coagulation function [APTT (activated partial thromboplastin time), FIB (fibrinogen), and PLT (platelet)], cytokines [CRP (C reactive protein), IL-6 (IL-6) and TNF-alpha (Tumor necrosis factor-alpha)] and T lymphocytes (CD4+, CD8+ and CD4+/CD8+) changes and clinical meaning of patients in the two groups.Results: The coagulation function related indicators, cytokines and T lymphocytes of the two groups before treatment did not change significantly (P>0.05). 1 d after operation, blood coagulation, TNF-alpha, CD4+ and CD4+/CD8+ levels were significantly lower than that before operation in two groups of patients (P<0.05), while IL-6, CRP and CD8+ were significantly higher than that before the operation (P<0.05), and the index change in open group was more obvious. 3 d after surgery, the APTT, IL-6, CRP, CD4+, CD8+ and PLT levels in two group patients were significantly lower than that 1 d after surgery, while FIB, TNF-alpha and CD4+/CD8+ were significantly higher than that 1 d after surgery;blood coagulation index, TNF-alpha and CD4+ and CD4+/CD8+ were significantly lower in the laparotomy group patients than in laparoscopic group, while IL-6, CRP and CD8+ were significantly higher than the laparoscopic group (P<0.05). 5 d after operation, the APTT, TNF-alpha, FIB, CD4+, CD4+/CD8+ and PLT in two groups were significantly higher than that 3 d after surgery (P<0.05), while IL-6, CRP and CD8+ levels were significantly lower than that of 3 d after surgery (P<0.05);blood coagulation index, TNF-alpha and CD4+ and CD4+/CD8+ in the laparotomy group patients were significantly lower than laparoscopic group (P<0.05), while IL-6, CRP and CD8+ were significantly higher than the laparoscopic group (P<0.05).Conclusions: Radical resection of gastric cancer can cause coagulation disorder, inhibit the immune function and inflammatory reaction, and gradually recover with the passage of time;Compared with open surgery, laparoscopic radical gastrectomy have smaller impact on patients and recovery faster.
基金supported by the National Natural Science Foundation of China(Nos.52170070,52400022 and 52200088)the Youth S&T Talent Support Programme of Guangdong Provincial Association for Science and Technology(GDSTA)(No.SKXRC202406)+1 种基金China Postdoctoral Science Foundation(No.2023M740754)“One hundred Youth”Science and Technology Plan,Guangdong University of Technology,China(No.263113906).
文摘As part of sewage treatment,coagulation could remove phosphorus from the effluent of the Anaerobic-Anoxic-Oxic-Anoxic(A^(2)OA)biological process.The importance in investigating the influence of coagulation on Anaerobic-Anoxic-Oxic-Anoxic Membrane Bioreactor(A^(2)OA-MBR)should be emphasized.In this study,systematic optimization of coagulation parameters for greater pollutant removal was conducted in terms of coagulant dosage,coagulation residence time and stirring hydraulic conditions.Coagulation process could remarkably remove turbidity,phosphorus,chemical oxygen demand,humic-like,protein-like and polysaccharide-like substances from secondary effluent and A^(2)OA sludge-liquid mixture.Furthermore,the influence of coagulation on membrane fouling development during the ultrafiltration of secondary effluent and A^(2)OA sludge-liquid mixture was investigated based on optimum coagulation parameters.Coagulation simultaneously reduced reversible membrane fouling and the irreversible one by 86%and 16%,respectively.According to excitation-emission matrix and attenuated total reflection-fourier transform infrared spectra,membrane fouling was primarily influenced by the cake layer,although pore fouling might be aggravated by A^(2)OA processes.Besides,the feasibility of coagulation-assisted A^(2)OA-MBRwas also assessed using hollowfibermembranes.It exhibited excellent potential in alleviatingmembrane fouling,while regular cleaning twice a day was not enough to suppress transmembrane pressure increase during direct domestic wastewater ultrafiltration.Additionally,both polysaccharide-like and protein-like foulants were vital components for membrane fouling during wastewater treatment.
基金Natural Science Foundation of Hubei Province of China,No.2024AFB655Key Research and Development Program of Hubei Province of China,No.2021BCA116National Natural Science Foundation of China,No.82072736,No.82003205,No.
文摘BACKGROUND Coagulation status is closely related to the progression of malignant tumors.In the era of neoadjuvant immunochemotherapy(NICT),the prognostic utility of coagulation indicators in patients with locally advanced gastric cancer(LAGC)undergoing new treatments remains to be determined.AIM To determine whether hypercoagulation is an effective prognostic indicator in patients with LAGC who underwent radical resection after NICT.METHODS A retrospective analysis of clinical data from 104 patients with LAGC,who underwent radical resection after NICT between 2020 and 2023,was performed.Ddimer and fibrinogen concentrations were measured one week before NICT,and again one week before surgery,to analyze the association between these two indicators and their combined indices[non-hypercoagulation(D-dimer and fibrinogen concentrations within the upper limit of normal)vs hypercoagulation(D-dimer or fibrinogen concentrations above the upper limit of normal)]with prognosis.After radical resection,patients were followed-up periodically.The median follow-up duration was 21 months.RESULTS Data collected after NICT revealed that the three-year overall survival(OS)and disease-free survival(DFS)rates the non-hypercoagulation group were significantly better than those in the hypercoagulation group[94.4%vs 78.0%(P=0.019)and 87.0%vs 68.0%(P=0.027),respectively].Multivariate analysis indicated that hypercoagulation after NICT was an independent factor for poor postoperative OS[hazard ratio(HR)4.436,P=0.023]and DFS(HR 2.551,P=0.039).Pre-NICT data demonstrated no statistically significant difference in three-year OS between the non-hypercoagulation and hypercoagulation groups(88.3%vs 84.1%,respectively;P=0.443).CONCLUSION Hypercoagulation after NICT is an effective prognostic indicator in patients with LAGC undergoing radical gastrectomy.
文摘BACKGROUND Currently,there is a notable lack of reliable studies evaluating the impact of multidisciplinary treatment strategies following transarterial chemoembolization(TACE)on patients with hepatocellular carcinoma(HCC),underscoring the urgent need for higher-level research in this area.AIM To investigate the association of multidisciplinary treatment strategies with the immunological,coagulation,and tumor biomarker responses after post-TACE in HCC.METHODS This retrospective analysis included 100 patients with HCC who were categorized based on the treatment approach into the control(patients treated with TACE alone)and experimental groups(patients receiving multidisciplinary treatment strategies post-TACE).Participant characteristics,short-term efficacy,and safety assessment as well as immunological,coagulation,and tumor biomarker res-ponses between the two groups were collected and compared.RESULTS Compared with the control group,the experimental group demonstrated a superior overall response rate,along with an increased fibrinogen,markedly improved immunological biomarker,lower prothrombin time,thrombin time,alpha-fetoprotein,carcinoembryonic antigen,and carbohydrate antigen 199 levels,as well as a decreased abnormal prothrombin incidence,and a lower overall rate of adverse reactions.Notably,no significant difference in the activated partial thromboplastin time and D-dimer levels was observed between the two groups.CONCLUSION Multidisciplinary treatment strategies post-TACE have improved the treatment outcome,the immunological response,and the coagulation function,lowered the tumor biomarker response levels,and reduced the risk of adverse reactions in patients with HCC.
文摘Gastric cancer(GC)has remained one of the leading causes of cancer-related deaths globally.The development of noninvasive biomarkers in cancer diagnosis and treatment has gained substantial traction in recent years.Recent evidence highlights hypercoagulation as a promising prognostic biomarker,particularly in locally advanced GC(LAGC)who underwent radical resection after neoadjuvant immunochemotherapy(NICT).A recent study by Li et al showed that hypercoagulation is a valuable prognostic indicator for patients with LAGC who have undergone radical resection following NICT.While the study addresses an important clinical issue and provides insightful findings,the present study offered valuable insights;the applicability of these findings was constrained by the retrospective design,the focus on a single center,and the small sample size of the existing studies.Additionally,vital confounders,such as preoperative comorbidities and systemic inflammation,are inadequately addressed.Future studies should focus on prospective multicenter trials,incorporating advanced predictive models such as machine learning algorithms to integrate coagulation markers with other clinical variables for personalized risk stratification.In addition,we are required to validate findings to examine the biological mechanisms correlating hypercoagulation to tumor progression.Integrating machine learning,comprehensive biomarker panels,and real-world data would allow the researchers to have personalized risk stratification,improve predictive accuracy,and optimize clinical decision-making.Finally,A multidisciplinary approach,including lifestyle interventions and imaging modalities,is essential to improve outcomes among patients with GC.
文摘Objective:To investigate the effects of intravenous thrombolysis therapy with alteplase on neurological function,coagulation function and serum inflammatory factors in patients with acute cerebral infarction.Methods:A total of 96 patients with acute cerebral infarction admitted to our hospital from September 2017 to October 2019 were randomly divided into two groups,with 48 patients in each group.The control group(n=48)received routine treatment,and the observation group received intravenous thrombolysis therapy with alteplase on the basis of routine treatment.The neurological deficit score,prothrombin time(PT),activated partial thromboplastin time(APTT),tumor necrosis factor-a level(TNF-α),and high-sensitivity C-reactive protein(hs-CRP)were compared between the two groups after 15 days of treatment.Results:After treatment,NIHSS scores in both groups were lower than those before treatment;PT levels were increased,while APTT,TNF-αand hs-CRP levels were all decreased in both groups,and the changes in the observation group were greater than those in the control group,with statistically significant difference(P<0.05).Conclusions:Intravenous thrombolysis therapy with alteplase can improve the neurological function,coagulation function and serum levels of inflammatory factors in patients with acute cerebral infarction,which is worthy of clinical application.
文摘BACKGROUND Colon polyps represent a significant clinical challenge in elderly patients.While endoscopic mucosal resection(EMR)and argon plasma coagulation(APC)are widely used,their comparative effectiveness in elderly populations remains unclear,particularly regarding postoperative recovery and complication profiles.AIM To compare the postoperative recovery,complications and efficacy of EMR and APC in elderly patients with colonic polyps.METHODS We retrospectively analyzed clinical data from 224 elderly patients with colon polyps treated at our center between January 2021 and July 2024.All patients were divided into the EMR group and APC group according to the surgical method they received.By comparing the operation time,intraoperative bleeding situation,hospital stays,postoperative inflammatory response index,complication rate and recurrence status of the two groups,the effect of the two surgical methods was comprehensively evaluated.RESULTS The APC group exhibited superior outcomes in terms of operative time(10.63 minutes vs 13.27 minutes,P<0.001),intraoperative bleeding situation(39.00%vs 52.42%,P=0.031),and length of hospital stay(1.63 days vs 3.87 days,P<0.001)compared to the EMR group.The one-time resection rate of the APC group(94.69%)was higher than that of the EMR group(89.14%)(P=0.026).The overall effective rates of the two groups were 94.35%and 92.00%,respectively.Postoperative procalcitonin and C-reactive protein levels were lower in the APC group than in the EMR group(P<0.001).The incidence of complications was comparable between the two groups(P=0.159).The recurrence rate was lower in the APC group(2.00%)than in the EMR group(8.06%)(P=0.045).CONCLUSION For elderly patients with colon polyps,APC showed certain advantages compared with EMR in promoting postoperative recovery,reducing the inflammatory response and the risk of complications.However,the study is limited by its single-center retrospective design and short follow-up period,and further multicenter prospective studies are needed to validate the findings.
