Sea Island cotton(Gossypium barbadense L.) has been highly valued in Verticillium wilt resistance and many fiber qualities including fiber length,strength,and fineness.To identify whether
The leukemia-associated autoinhibitor (LAI-615) derived from murine leukemia L7811 has been investigated intensively in our laboratory. In the following experiments, the partial purification of LA I-615 has been carri...The leukemia-associated autoinhibitor (LAI-615) derived from murine leukemia L7811 has been investigated intensively in our laboratory. In the following experiments, the partial purification of LA I-615 has been carried out in addition to the observation of phenotype variations of L7811 leuke-mic cells. The factor was purified over 1306-fold by sequential fractionation with Sephadex G-150 gel filtration, DEAE-cellulose ion exchange chromato-graphy, and Mono Q-fast protein liquid chromato-graphy. The molecular weight of LAI-615 was 68,000 as estimated by gel filtration. LAI-615 was a protein but not glycosylated, and it was suggested LAI-615 be secreted in an autocrine manner. Im-munocytochemical staining showed that the expression of Lyt2 phenotype of L7811 leukemic cells was often coincident with the secretion of LAI-615. Moreover, the physicochemical characteristics of LAI-615 was similar to that of T suppressor factor. Thus it is concluded that LAI-615 may be one of TsF-like factors.展开更多
Conventional ecological risk assessments prioritize downstream anthropogenic impacts,overlooking risks arising from upstream processes involving highly hazardous substances and indirect emissions.This narrow focus obs...Conventional ecological risk assessments prioritize downstream anthropogenic impacts,overlooking risks arising from upstream processes involving highly hazardous substances and indirect emissions.This narrow focus obscures high-risk hotspots and renders traditional methodologies ill-suited for evaluating novel chemical entities.Nanopesticides,designed for targeted delivery of pesticidal active ingredients,are increasingly deployed to enhance efficiency,yet their altered environmental fate and transport dynamics may reshape end-of-life risks while their full lifecycle impacts remain uncharacterized.Here,we address this gap using imidacloprid(IMI)and its nano-encapsulated variant(nano-IMI)as case studies.By applying life cycle assessment and integrating the USEtox ecotoxicity model with the nano-specific SimpleBox4Nano framework,we quantify"cradle-to-gate"environmental impacts and derive substance-specific ecotoxicity metrics,enabling systematic characterization of end-of-life risks associated with these formulations.Production-stage ecological risks of nano-IMI(4.63×10^(3)CTUe)are approximately four times greater than those for conventional IMI(1.18×10^(3) CTUe).However,end-of-life freshwater ecological risks from nano-IMI emissions(0.012-6.93×10^(4) CTUe)are 2-5 orders of magnitude lower compared with IMI(1.59×10^(3) -6.13×10^(4) CTUe),accounting for rainfall variability,toxicity data selection,fate,and environmental transport scenarios.Under equivalent rainfall conditions,nano-IMI exhibited up to three orders of magnitude lower integrated life-cycle freshwater ecological risks,underscoring its potential as an environmentally preferable alternative to conventional IMI.This research introduces a comprehensive and novel methodology for evaluating engineered nanomaterial alternatives across realistic environmental scenarios,providing essential insights into nanopesticide risk assessment throughout their lifecycle.展开更多
文摘Sea Island cotton(Gossypium barbadense L.) has been highly valued in Verticillium wilt resistance and many fiber qualities including fiber length,strength,and fineness.To identify whether
文摘The leukemia-associated autoinhibitor (LAI-615) derived from murine leukemia L7811 has been investigated intensively in our laboratory. In the following experiments, the partial purification of LA I-615 has been carried out in addition to the observation of phenotype variations of L7811 leuke-mic cells. The factor was purified over 1306-fold by sequential fractionation with Sephadex G-150 gel filtration, DEAE-cellulose ion exchange chromato-graphy, and Mono Q-fast protein liquid chromato-graphy. The molecular weight of LAI-615 was 68,000 as estimated by gel filtration. LAI-615 was a protein but not glycosylated, and it was suggested LAI-615 be secreted in an autocrine manner. Im-munocytochemical staining showed that the expression of Lyt2 phenotype of L7811 leukemic cells was often coincident with the secretion of LAI-615. Moreover, the physicochemical characteristics of LAI-615 was similar to that of T suppressor factor. Thus it is concluded that LAI-615 may be one of TsF-like factors.
基金supported by the National Natural Science Foundation of China(42107286)the Natural Science Foundation of Guangdong Province(2023A1515011215)+2 种基金Guangzhou Basic and Applied Basic Research Foundation(2024A04J9882)the Fundamental Research Funds for the Central Universities(21623214)the Department of Education of Guangdong Province(2020KCXTD005).
文摘Conventional ecological risk assessments prioritize downstream anthropogenic impacts,overlooking risks arising from upstream processes involving highly hazardous substances and indirect emissions.This narrow focus obscures high-risk hotspots and renders traditional methodologies ill-suited for evaluating novel chemical entities.Nanopesticides,designed for targeted delivery of pesticidal active ingredients,are increasingly deployed to enhance efficiency,yet their altered environmental fate and transport dynamics may reshape end-of-life risks while their full lifecycle impacts remain uncharacterized.Here,we address this gap using imidacloprid(IMI)and its nano-encapsulated variant(nano-IMI)as case studies.By applying life cycle assessment and integrating the USEtox ecotoxicity model with the nano-specific SimpleBox4Nano framework,we quantify"cradle-to-gate"environmental impacts and derive substance-specific ecotoxicity metrics,enabling systematic characterization of end-of-life risks associated with these formulations.Production-stage ecological risks of nano-IMI(4.63×10^(3)CTUe)are approximately four times greater than those for conventional IMI(1.18×10^(3) CTUe).However,end-of-life freshwater ecological risks from nano-IMI emissions(0.012-6.93×10^(4) CTUe)are 2-5 orders of magnitude lower compared with IMI(1.59×10^(3) -6.13×10^(4) CTUe),accounting for rainfall variability,toxicity data selection,fate,and environmental transport scenarios.Under equivalent rainfall conditions,nano-IMI exhibited up to three orders of magnitude lower integrated life-cycle freshwater ecological risks,underscoring its potential as an environmentally preferable alternative to conventional IMI.This research introduces a comprehensive and novel methodology for evaluating engineered nanomaterial alternatives across realistic environmental scenarios,providing essential insights into nanopesticide risk assessment throughout their lifecycle.
