Cold stress in low-temperature environments can trigger changes in gene expression,but epigenomics regulation of temperature stability in vital tissues,including the fat and diencephalon,is still unclear.Here,we explo...Cold stress in low-temperature environments can trigger changes in gene expression,but epigenomics regulation of temperature stability in vital tissues,including the fat and diencephalon,is still unclear.Here,we explore the cold-induced changes in epigenomic features in the diencephalon and fat tissues of two cold-resistant Chinese pig breeds,Min and Enshi black(ES)pigs,utilizing H3K27ac CUT&Tag,RNA-seq,and selective signature analysis.Our results show significant alterations in H3K27ac modifications in the diencephalon of Min pigs and the fat of ES pigs after cold exposure.Dramatic changes in H3K27ac modifications in the diencephalon of Min pig are primarily associated with genes involved in energy metabolism and hormone regulation,whereas those in the fat of ES pig are primarily associated with immunity-related genes.Moreover,transcription factors PRDM1 and HSF1,which show evidence of selection,are enriched in genomic regions presenting cold-responsive alterations in H3K27ac modification in the Min pig diencephalon and ES pig fat,respectively.Our results indicate the diversity of epigenomic response mechanisms to cold exposure between Min and ES pigs,providing unique epigenetic resources for studies of low-temperature adaptation in large mammals.展开更多
Swine mycoplasma pneumonia caused by Mycoplasma hyopneumoniae is the most prevalent and frequently occurring chronic respiratory disease in pigs worldwide.Pig breeds in China are more susceptible to Mycoplasma hyopneu...Swine mycoplasma pneumonia caused by Mycoplasma hyopneumoniae is the most prevalent and frequently occurring chronic respiratory disease in pigs worldwide.Pig breeds in China are more susceptible to Mycoplasma hyopneumoniae,and understanding the reasons for their susceptibility is crucial for the prevention and control of swine Mycoplasma pneumonia,as well as for promoting the healthy development of the swine industry.Mycoplasma hyopneumoniae has limited biosynthesis and metabolism,and has harsh requirements for nutrients,thus it is necessary to add approximately 15%-20%porcine serum in medium during in vitro culture,and porcine serum plays an irreplaceable role in the growth and reproduction of Mycoplasma hyopneumoniae.In this study,growth titres of different Mycoplasma hyopneumoniae strains cultured in the serum medium from different Chinese pig breeds and introduced pig breeds were compared.Non-targeted metabolomics analysis of representative introduced and Chinese pig breeds,Large white pig and Bama miniature pig,respectively,screened of key serum metabolites.The results showed that,expression level of L-Asparagine in Bama miniature pigs was 15–18 times higher than in Large white pigs.Additionally,the contents of inositol and Arachidonic acid in Bama miniature pigs were significantly upregulated as well.A certain concentration of L-Asparagine significantly increased the growth titer,metabolic capacity and pathogenicity of Mycoplasma hyopneumoniae.In conclusion,Bama miniature pig and Large white pig are representative pig breeds that are susceptible and non-susceptible to Mycoplasma hyopneumoniae respectively.L-Asparagine is the essential factor and biomarker for the susceptibility of Chinese pigs to Mycoplasma hyopneumoniae.展开更多
基金supported by the National Natural Science Foundation of China(32202634)National Natural Science Foundation of China(31961143020)+2 种基金National Natural Science Foundation of China(U20A2052)China Agricultural Research System(CARS-35)the Heilongjiang Provincial Research Institutes Research Business Fund Project(CZKYF2023-1-B009)。
文摘Cold stress in low-temperature environments can trigger changes in gene expression,but epigenomics regulation of temperature stability in vital tissues,including the fat and diencephalon,is still unclear.Here,we explore the cold-induced changes in epigenomic features in the diencephalon and fat tissues of two cold-resistant Chinese pig breeds,Min and Enshi black(ES)pigs,utilizing H3K27ac CUT&Tag,RNA-seq,and selective signature analysis.Our results show significant alterations in H3K27ac modifications in the diencephalon of Min pigs and the fat of ES pigs after cold exposure.Dramatic changes in H3K27ac modifications in the diencephalon of Min pig are primarily associated with genes involved in energy metabolism and hormone regulation,whereas those in the fat of ES pig are primarily associated with immunity-related genes.Moreover,transcription factors PRDM1 and HSF1,which show evidence of selection,are enriched in genomic regions presenting cold-responsive alterations in H3K27ac modification in the Min pig diencephalon and ES pig fat,respectively.Our results indicate the diversity of epigenomic response mechanisms to cold exposure between Min and ES pigs,providing unique epigenetic resources for studies of low-temperature adaptation in large mammals.
基金supported by National Natural Science Foundation of China(32273011,32172860)Jiangsu Agriculture Science and Technology Innovation Fund(CX(22)3195)+1 种基金Food Quality and Safety-State Key Laboratory Cultivation Base,Ministry of Science and Technology,China(2022-SBGJZZ-6)Jiangsu Association for Science and Technology Youth Science and Technology Talents Support Project(JSTJ-2023-093).
文摘Swine mycoplasma pneumonia caused by Mycoplasma hyopneumoniae is the most prevalent and frequently occurring chronic respiratory disease in pigs worldwide.Pig breeds in China are more susceptible to Mycoplasma hyopneumoniae,and understanding the reasons for their susceptibility is crucial for the prevention and control of swine Mycoplasma pneumonia,as well as for promoting the healthy development of the swine industry.Mycoplasma hyopneumoniae has limited biosynthesis and metabolism,and has harsh requirements for nutrients,thus it is necessary to add approximately 15%-20%porcine serum in medium during in vitro culture,and porcine serum plays an irreplaceable role in the growth and reproduction of Mycoplasma hyopneumoniae.In this study,growth titres of different Mycoplasma hyopneumoniae strains cultured in the serum medium from different Chinese pig breeds and introduced pig breeds were compared.Non-targeted metabolomics analysis of representative introduced and Chinese pig breeds,Large white pig and Bama miniature pig,respectively,screened of key serum metabolites.The results showed that,expression level of L-Asparagine in Bama miniature pigs was 15–18 times higher than in Large white pigs.Additionally,the contents of inositol and Arachidonic acid in Bama miniature pigs were significantly upregulated as well.A certain concentration of L-Asparagine significantly increased the growth titer,metabolic capacity and pathogenicity of Mycoplasma hyopneumoniae.In conclusion,Bama miniature pig and Large white pig are representative pig breeds that are susceptible and non-susceptible to Mycoplasma hyopneumoniae respectively.L-Asparagine is the essential factor and biomarker for the susceptibility of Chinese pigs to Mycoplasma hyopneumoniae.
