Lignin-degrading enzymes secreted by white rot fungi play an important role in the degradation of lignin and persistent organic pollutants(POPs).In this study,effect of environmental C/N ratio on the activities of lig...Lignin-degrading enzymes secreted by white rot fungi play an important role in the degradation of lignin and persistent organic pollutants(POPs).In this study,effect of environmental C/N ratio on the activities of lignin-degrading enzymes,lignin peroxide(Li P)and manganese peroxidase(Mn P),produced by Phanerochaete chrysosporium,a white rot fungus,was investigated.Glucose was used as C source,and ammonium tartrate of different concentrations was used as N source to provide different C/N ratios.Relationships between Li P and Mn P activities and environmental C/N ratio were explored.The results showed that the higher the N source concentration,the faster the mycelium pellets aged.The faster the mycelium dry weight increased,the higher the Li P and Mn P activities.A high C/N ratio was a necessary condition for the secretion of Li P or Mn P.In addition,mycelium dry weight essentially affected enzyme activities.In the 122 C/N ratio and 50 C/N ratio treatments,mycelium dry weight essentially affected Mn P activity and both Li P and Mn P activities,respectively.展开更多
基金supported by the National Natural Science Foundation of China(Nos.41171250,20907011,and 41401356)the Natural Science Foundation of Guangdong Province,China(No.2014A030313702)。
文摘Lignin-degrading enzymes secreted by white rot fungi play an important role in the degradation of lignin and persistent organic pollutants(POPs).In this study,effect of environmental C/N ratio on the activities of lignin-degrading enzymes,lignin peroxide(Li P)and manganese peroxidase(Mn P),produced by Phanerochaete chrysosporium,a white rot fungus,was investigated.Glucose was used as C source,and ammonium tartrate of different concentrations was used as N source to provide different C/N ratios.Relationships between Li P and Mn P activities and environmental C/N ratio were explored.The results showed that the higher the N source concentration,the faster the mycelium pellets aged.The faster the mycelium dry weight increased,the higher the Li P and Mn P activities.A high C/N ratio was a necessary condition for the secretion of Li P or Mn P.In addition,mycelium dry weight essentially affected enzyme activities.In the 122 C/N ratio and 50 C/N ratio treatments,mycelium dry weight essentially affected Mn P activity and both Li P and Mn P activities,respectively.
