Spider mites are significant pests in agricultural production.The increasing resistance of spider mites,along with environmental pollution and ecological imbalance caused by their control,is primarily attributed to th...Spider mites are significant pests in agricultural production.The increasing resistance of spider mites,along with environmental pollution and ecological imbalance caused by their control,is primarily attributed to the long-term use of chemical acaricides in agriculture.In contrast,bioactive substances of biological origin offer advantages such as wide availability,environmental friendliness,and low tendency to induce resistance,making them a research hotspot for spider mite control.This review summarizes recent advances in the use of plant-derived active compounds(exemplified by extracts from Veratrum rhizomes),RNA interference(RNAi)technology,and microorganism-derived active substances for controlling spider mites.These bioactive agents exert acaricidal effects by disrupting the nervous system,interfering with metabolic processes,or silencing key genes in mites,demonstrating favorable efficacy and considerable potential for development.However,challenges remain,including poor environmental stability,slow action,high production costs,and insufficient understanding of their effects on non-target organisms.Therefore,future research should focus on the screening and development of novel bioactive substances of biological origin,elucidation of their mechanisms of action,optimization of formulation technologies,and assessment of their ecological safety.These efforts will provide valuable insights for promoting the advancement of bioactive substances and supporting sustainable agricultural development.展开更多
Obesity and diabetes have become global health crises,with rising prevalence and a strong association with various chronic diseases such as cardiovascular disease,stroke,and certain cancers.These conditions contribute...Obesity and diabetes have become global health crises,with rising prevalence and a strong association with various chronic diseases such as cardiovascular disease,stroke,and certain cancers.These conditions contribute to significant morbidity and mortality,highlighting the urgent need for effective therapeutic interventions.Marine products,including fish oils and marine plants,have been increasingly recognized for their potential in mitigating these diseases.Among these,seaweed stands out due to their diverse bioactive compounds and promising therapeutic effects.This comprehensive review explores the mechanisms of action through which seaweeds,and their compounds exert anti-diabetic and anti-obesity effects,including the regulation of adipogenesis,appetite control,modulation of gut microbiota,enhancement of insulin sensitivity,and reduction of inflammation,oxidative stress,andβ-cell dysfunction.Despite the promising potential,challenges such as variability of bioactive compounds and low bioavailability remain there.Advances in bioactive delivery systems and along with large-scale clinical trials,are crucial for optimizing the therapeutic use of bioactive compounds from seaweeds.Future research should also explore synergistic strategies combining seaweed compounds with other bioactive substances.Overall,seaweed offers a promising foundation for developing functional foods and nutraceuticals aimed at promoting long-term metabolic health,providing an innovative approach to addressing obesity and diabetes.展开更多
In this study,injectable bone graft putty samples were developed using fine and coarse melt-quenched 45S5 bioactive glass(BG)incorporated into a carrier system composed of glycerol and polyethylene glycol(PEG)with dif...In this study,injectable bone graft putty samples were developed using fine and coarse melt-quenched 45S5 bioactive glass(BG)incorporated into a carrier system composed of glycerol and polyethylene glycol(PEG)with different average molecular weights.Selected putty samples were further incorporated with varying amounts of Denosumab(5wt%-10wt%)to investigate its influence on rhe-ological behavior and flow properties using mathematical modeling.All PEG/glycerol/45S5-based putty samples exhibited viscoelastic behavior(storage modulus>loss modulus)and pseudoplastic behavior(n<1),with viscosity values required for optimal flow remaining below 1000 Pa∙s.Both viscosity and thixotropic area increased proportionally with higher BG content and smaller-sized BG particles.All putty samples showed more than 98%injectability through a 12G cannula,suggesting potential clinical suitability.However,injectability decreased with smaller cannulas,dropping to 34.7%-58.3%with a 19G cannula and further decreasing with a 23G cannula at higher BG contents.Incorporation of Denosumab preserved viscoelasticity and injectability but modified the flow behavior,shifting it from pseudo-plastic to more Newtonian with higher Denosumab content,while also reducing viscosity and thixotropic area values.Among all tested samples,putty containing a lower amount of Denosumab and smaller-sized BG exhibited the most suitable combination of injectability and rheological features.All putty samples were well described by both the Power law and Herschel-Bulkley rheological models(coeffi-cient of determination>0.95).This study highlights the influence of Denosumab on flowability and rheological relationships and sug-gests potential improvements in bioactivity through a dual synergistic effect of BG and Denosumab in minimally invasive bone graft sys-tems.展开更多
Agastache rugosa,a medicinal plant known for its bioactive compounds,has gained attention for its pharmacological and commercial potential.This study aimed to optimize ethanol concentration to enhance growth and bioac...Agastache rugosa,a medicinal plant known for its bioactive compounds,has gained attention for its pharmacological and commercial potential.This study aimed to optimize ethanol concentration to enhance growth and bioactive compound production in A.rugosa cultivated in a controlled plant factory system.Ethanol treatments at 40 and 80 mM significantly promoted both vegetative and reproductive growth.Plants treated with these concentrations exhibited higher net photosynthetic rates(A)and intercellular CO_(2) concentration(Ci)compared to the untreated control,whereas stomatal conductance(gs)and transpiration rate(E)remained unaffected.Chlorophyll and carotenoid concentrations,and SPAD values,significantly increased with ethanol treatment.Total flavonoid and total phenolic contents as well as 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical-scavenging activities were significantly higher in plants treated with ethanol than in the untreated control.Ethanol treatments led to a significant enhancement in the activities of antioxidant enzymes,including superoxide dismutase,peroxidase,and catalase.Furthermore,ethanol treatment elevated rosmarinic acid concentrations in roots and tilianin and acacetin levels in flowers.Collectively,ethanol at 40 and 80 mM effectively enhanced growth,photosynthesis,antioxidant defense,and bioactive compound production in A.rugosa cultivated in a plant factory.These findings provide valuable insights for improving cultivation of medicinal plants with high pharmaceutical and nutraceutical value.展开更多
Roasting is important for improving the content of bioactive compounds and enhancing the flavor of flaxseed oil.However,the effect of roasting time on germinated flaxseed oil(GFO)is unclear.Herein,the influence of roa...Roasting is important for improving the content of bioactive compounds and enhancing the flavor of flaxseed oil.However,the effect of roasting time on germinated flaxseed oil(GFO)is unclear.Herein,the influence of roasting time(0–50 min)at a specific temperature of 160?C on the physicochemical properties,bioactive compounds,antioxidant capacity,and volatile compounds of GFO from three flaxseed varieties was examined.An extension in roasting time increased the a^(*)value of GFO,while the L^(*)and b^(*)values of GFO decreased.Higher chlorophylls(1.63±0.01–2.18±0.01 mg/kg),carotenoids(2.25±0.00–2.69±0.02 mg/kg),total phenolic content(156.75±2.93–200.03±6.29 mg GAE/kg),phytosterol(1801.93±30.64–1965.23±13.71 mg GAE/kg)and DPPH(320.11±18.22–432.19±14.95μmol TE/kg)and ABTS radical scavenging activity(810.68±16.29–1119.01±14.25μmol TE/kg)were detected in GFO after roasting for 20–30 min.However,the total tocopherol and unsaturated fatty acid content declined,and the composition of fatty acids changed insignificantly(P>0.05).GC–MS recognized 68 volatile compounds in GFO,in which pyrazine imparted a nutty flavor to the GFO.This study provides important information for the development of high-quality GFO.展开更多
The cosmetic sector is a multibillion-dollar industry that requires constant attention being paid to innovative product development and engagement.Notably,its market value is projected to exceed 750 billion U.S.dollar...The cosmetic sector is a multibillion-dollar industry that requires constant attention being paid to innovative product development and engagement.Notably,its market value is projected to exceed 750 billion U.S.dollars by 2025,and it is expanding as novel,climate-friendly,green,and sustainable components from natural sources are incorporated.This review is written based on the numerous reports on the potential applications of food-derived peptides while focusing on their possible uses in the formulation of cosmeceutical and skincare products.First,the production methods of bioactive peptides linked to cosmeceutical uses are described.Then,we discuss the obtainment and characterization of different anti-inflammatory,antimicrobial,antioxidant,anti-aging,and other pleiotropic peptides with their specific mechanisms,from various food sources.The review concludes with salient considerations of the cost of production and pilot scale operation,stability,compatibility,user safety,site-specificity,and delivery methods,when designing or developing biopeptide-based cosmeceutical products.展开更多
Introduction The global burden of orthopedic diseases has reached unprecedented levels,with recent epidemiological data revealing that musculoskeletal conditions affect over 1.71 billion people worldwide,representing ...Introduction The global burden of orthopedic diseases has reached unprecedented levels,with recent epidemiological data revealing that musculoskeletal conditions affect over 1.71 billion people worldwide,representing a 150%increase since 1990[1].By 2050,the number of individuals aged 60 and above requiring orthopedic interventions is projected to exceed 2.1 billion,with osteoporosis alone affecting 200 million people globally.展开更多
In this study,hydrophobically modified phytoglycogen and hydrolyzed zein were utilized to develop biodegradable and redispersible nanocomplexes for encapsulation of various bioactive phytochemicals,including eugenol,t...In this study,hydrophobically modified phytoglycogen and hydrolyzed zein were utilized to develop biodegradable and redispersible nanocomplexes for encapsulation of various bioactive phytochemicals,including eugenol,thymol,carvacrol,trans-cinnamaldehyde,and their mixtures.Comprehensive physicochemical and colloidal characterizations of these nanocomplexes were performed using dynamic light scattering,and Fourier transform infrared and atomic force microscopy,which evaluated their colloidal stability and redispersibility after freeze-drying.In addition,the antioxidant capacities and antimicrobial activities of these various nanoparticles were investigated methodically.The results revealed that the phytochemical-encapsulated nanocomplexes exhibited a particle size ranging from 60 to 70 nm,display-ing a uniform distribution.Additionally,these nanocomplexes demonstrated sustained morphological and colloidal stability over 30-day storage,and after freeze-drying and subsequent redispersion processing.Furthermore,significant improvements in antioxidant and antimicrobial properties in the aqueous phase were observed.These redispersible nanocomplexes from phytoglycogen and zein hold the promising potential to address the limitations and offer practical solutions for expanding their applications in the pharmaceutical and food fields.展开更多
Pu-erh tea,a traditional Chinese beverage,performs an anti-obesity function,but the correlation between its components and efficacy remains unknown.Here,we screened two Pu-erh teas with significant anti-obesity effica...Pu-erh tea,a traditional Chinese beverage,performs an anti-obesity function,but the correlation between its components and efficacy remains unknown.Here,we screened two Pu-erh teas with significant anti-obesity efficacies from 11 teas.In vitro experiments revealed that lipid accumulation in L02 cells and lipid synthesis in 3T3-L1 cells were significantly better inhibited by Tea-B than Tea-A.Further in vivo experiments using model mice revealed that the differences in chemical components generated two pathways in the anti-obesity efficacy and mechanism of Pu-erh teas.Tea-A changes the histomorphology of brown adipose tissue(BAT)and increases the abundance of Coriobacteriaceae_UCG_002 and cyclic AMP in guts through high chemical contents of cyclopentasiloxane,decamethyl,tridecane and 1,2,3-trimethoxybenzene,eventually increasing BAT activation and fat browning gene expression;the high content of hexadecane and 1,2-dimethoxybenzene in Tea-B reduces white adipose tissue(WAT)accumulation and the process of fatty liver,increases the abundance of Odoribacter and sphinganine 1-phosphate,inhibits the expression of lipid synthesis and transport genes.These mechanistic findings on the association of the representative bioactive components in Pu-erh teas with the anti-obesity phenotypes,gut microbes,gut metabolite structure and anti-obesity pathways,which were obtained for the first time,provide foundations for developing functional Pu-erh tea.展开更多
Bacterial infections have seriously impaired the wound healing.Despite numerous antibiotics have been developed to inhibit bacteria,the emergence of antibiotic resistance necessitates a rethinking of the public health...Bacterial infections have seriously impaired the wound healing.Despite numerous antibiotics have been developed to inhibit bacteria,the emergence of antibiotic resistance necessitates a rethinking of the public health crisis.In this study,we introduce the carbon dots derived from metformin and dopamine(MD-CDs)as a novel approach to promote the healing of bacterial-infected wound.Through a hydrothermal reaction,the regularly spherical MD-CDs were prepared.Owing to their inherent antibacterial property,MD-CDs effectively inhibited the wound infection and expedited the transition from the inflammation phase to the healing phase in infected wound.Additionally,MD-CDs enhanced the proliferation and migration of L929 cells,which is crucial for wound tissue regeneration.Following the treatment of infected fullthickness skin wounds,MD-CDs effectively promoted the collagen deposition and regeneration of hair follicle in the healing phase.Consequently,MD-CDs represent a bioactive nanomaterial exhibiting inherent antibacterial,offering a promising alternative for the treatment of infected wounds.展开更多
The carbonization processing(Paozhi)in traditional Chinese medicine(TCM)represents a unique pharmaceutical technology where thermal modification of herbal materials enhances specific therapeutic properties,particularl...The carbonization processing(Paozhi)in traditional Chinese medicine(TCM)represents a unique pharmaceutical technology where thermal modification of herbal materials enhances specific therapeutic properties,particularly hemostatic and antioxidant effects.Despite centuries of empirical applications,the scientific basis underlying these enhanced bioactivities remains poorly characterized,particularly regarding the transformation and functionalization of active components during high-temperature carbonization.This study systematically investigates carbon dots(CDs),emerging carbon-based nanomaterials spontaneously formed during the carbonization process,as potential key bioactive constituents mediating the therapeutic actions of carbonized TCM.Through multidisciplinary analysis of pyrolysis-driven CD formation mechanisms,nanostructural evolution,and surface chemistry modulation,we demonstrate that CDs exhibit size-dependent fluorescence properties and redox-active surface functional groups that correlate with their observed biological effects.Crucially,the study establishes quantitative structure-activity relationships between CDs’quantum confinement characteristics(2–8 nm diameter),oxygencontaining surface moieties(carboxyl,hydroxyl groups),and their procoagulant/antioxidant capacities.By bridging traditional processing knowledge with nanotechnology insights,this work not only deciphers the“black box”of thermal processing in TCM but also proposes a nano-biointerface paradigm for understanding Paozhi mechanisms.The findings advance quality control strategies through CD-based spectral fingerprinting and open new avenues for developing nanoscale TCM derivatives with optimized therapeutic profiles.展开更多
Angelica sinensis,a well-known traditional Chinese medicinal herb with the unique property of being both a medicine and an edible plant,has been widely used for promoting blood circulation,modulating immunity,and reli...Angelica sinensis,a well-known traditional Chinese medicinal herb with the unique property of being both a medicine and an edible plant,has been widely used for promoting blood circulation,modulating immunity,and relieving pain.This review comprehensively investigates the extraction methods,structural characteristics,and biological activities of its primary bioactive components,such as polysaccharides,volatile oils,organic acids,and flavonoids.The biosynthesis pathways of these compounds,along with the key enzymes and transcription factors involved,are investigated to understand the factors influencing their synthesis and accumulation.Additionally,the biological activities of A.sinensis,including hepatoprotective,anti-inflammatory,immunemodulatory,anti-tumor,circulatory benefits,and neuroprotection,along with their underlying mechanisms are introduced.These findings provide a solid foundation for the development of A.sinensis as a valuable resource in functional foods and pharmaceutical products.展开更多
Bioactive peptides and proteins(BAPPs)are promising therapeutic agents for tissue repair with considerable advantages,including multifunctionality,specificity,biocompatibility,and biodegradability.However,the high com...Bioactive peptides and proteins(BAPPs)are promising therapeutic agents for tissue repair with considerable advantages,including multifunctionality,specificity,biocompatibility,and biodegradability.However,the high complexity of tissue microenvironments and their inherent deficiencies such as short half-live and susceptibility to enzymatic degradation,adversely affect their therapeutic efficacy and clinical applications.Investigating the fundamental mechanisms by which BAPPs modulate the microenvironment and developing rational delivery strategies are essential for optimizing their administration in distinct tissue repairs and facilitating clinical translation.This review initially focuses on the mechanisms through which BAPPs influence the microenvironment for tissue repair via reactive oxygen species,blood and lymphatic vessels,immune cells,and repair cells.Then,a variety of delivery platforms,including scaffolds and hydrogels,electrospun fibers,surface coatings,assisted particles,nanotubes,two-dimensional nanomaterials,and nanoparticles engineered cells,are summarized to incorporate BAPPs for effective tissue repair,modification strategies aimed at enhancing loading efficiencies and release kinetics are also reviewed.Additionally,the delivery of BAPPs can be precisely regulated by endogenous stimuli(glucose,reactive oxygen species,enzymes,pH)or exogenous stimuli(ultrasound,heat,light,magnetic field,and electric field)to achieve on-demand release tailored for specific tissue repair needs.Furthermore,this review focuses on the clinical potential of BAPPs in facilitating tissue repair across various types,including bone,cartilage,intervertebral discs,muscle,tendons,periodontal tissues,skin,myocardium,nervous system(encompassing brain,spinal cord,and peripheral nerve),endometrium,as well as ear and ocular tissue.Finally,current challenges and prospects are discussed.展开更多
Oxidative stress is caused by an imbalance of the antioxidant defense system and excessive production of free radicals,which can damage biological macromolecules such as proteins,lipids and nucleic acids,and can cause...Oxidative stress is caused by an imbalance of the antioxidant defense system and excessive production of free radicals,which can damage biological macromolecules such as proteins,lipids and nucleic acids,and can cause aging,ischemia-reperfusion injury,inflammatory injury,liver and kidney injury and other diseases.The nuclear factor erytheroid-2-related factor 2(Nrf2)is a major regulator of redox balance.The Nrf2 pathway also exerts a central function in cell apoptosis,oxidative stress damage and metabolic diseases.Bioactive peptides are small molecular peptides composed of amino acid residues.They have many biological activities and play important physiological roles in human body.Antioxidant peptide is a kind of peptide which can reduce oxidative stress damage.They are safe,non-toxic and easily absorbed.In this review,we summarized the mechanism of bioactive peptides in regulating oxidative stress,especially antioxidant peptides,through regulating the Nrf2 signaling pathway and the expressions of oxidative stress-related genes,to alleviate oxidative stress-induced damage.The paper will provide valuable reference to investigators in the antioxidant peptide field and also promote the applications of antioxidant peptides in the oxidative stress-associated diseases.展开更多
Cold atmospheric plasmas are widely used in biomedicine.Although direct plasma treatments of wounds have been demon-strated,there are still obstacles hampering further clinical adoption,for example,the limited treatme...Cold atmospheric plasmas are widely used in biomedicine.Although direct plasma treatments of wounds have been demon-strated,there are still obstacles hampering further clinical adoption,for example,the limited treatment area,inconsistent ac-tions and risk of thermal injury.In this respect,plasma-activated air(PAA)is proposed and demonstrated for infected wounds treatment as an alternative to the conventional direct plasma treatment.The combination of gliding arc discharge reactor and dielectric barrier discharge reactor produces highly bioactive PAA.In in vitro sterilisation of Staphylococcus aureus,approxi-mately 9-log reduction is achieved after the PAA treatment for 6 min.Bovine serum albumin is added to the S.aureus sus-pension to further simulate the wound exudate to accomplish inactivation of approximately 3-log reduction after 10 min.In vivo experiments show that the PAA treatment of infected wounds significantly reduces the bacterial load and improves the healing rate,revealing an optimal treatment time of 3 min/day.The immunohistochemical and blood biochemical analyses show that the PAA-3 min treatment enhances wound healing by inhibiting the tissue inflammatory response and inducing growth factor production without showing evident systemic toxicity.In conclusion,PAA holds great clinical promise as a safe and effective wound-healing strategy.展开更多
Diabetic wounds(DWs)are a major complication of diabetes mellitus,characterized by a complex patho-physiological microenvironment that is associated with elevated morbidity and mortality.Conventional management strate...Diabetic wounds(DWs)are a major complication of diabetes mellitus,characterized by a complex patho-physiological microenvironment that is associated with elevated morbidity and mortality.Conventional management strategies often fail to address the multifaceted nature of these wounds effectively.Recent advancements in understanding the mechanisms of DW healing have spurred the development of a plethora of bioactive dressings designed to interact with and modulate the DW microenvironment.These innovations have culminated in the introduction of the“microenvironment-sensitive with on-demand management”paradigm aimed at delivering precision therapy responsive to dynamic changes within DW.Despite these advancements,the current literature lacks a comprehensive review that cate-gorizes and evaluates active,passive,and on-demand approaches that address the DW microenviron-ment.Herein,we describe the unique pathogenic mechanisms and microenvironmental characteristics that distinguish DW from normal acute wounds.This review provides an extensive overview of contem-porary active and passive management strategies incorporating on-demand management principles designed for DW microenvironments.Furthermore,it addresses the principal challenges faced in this therapeutic domain and outlines the potential innovations that can enhance the efficacy and specificity of bioactive dressings.The insights presented here aim to guide further research and development in the on-demand management of DW to improve patient outcomes by aligning personalized therapy modali-ties with the pathophysiological realities of DW.展开更多
Natural products are great treasure troves for the discovery of bioactive components.Current bioassay guided fractionation for identification of bioactive components is time-and workload-consuming.In this study,we pro...Natural products are great treasure troves for the discovery of bioactive components.Current bioassay guided fractionation for identification of bioactive components is time-and workload-consuming.In this study,we proposed a robust and convenient strategy for deciphering the bioactive profile of natural products by mass spectral molecular networking combined with rapid bioassay.As a proof-of-concept,the strategy was applied to identify angiotensin converting enzyme(ACE)inhibitors of Fangjihuangqi decoction(FJHQD),a traditional medicine clinically used for the treatment of heart failure.The chemical profile of FJHQD was comprehensively revealed with the assistance of tandem mass spectral molecular networking,and a total of 165 compounds were identified.With characterized constituents,potential clinical applications of FJHQD were predicted by Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine,and a range of cardiovascular related diseases were significantly enriched.ACE inhibitory activities of FJHQD and its constituents were then investigated with an aggregation-induced emission based fluorescent probe.FJHQD exhibited excellent ACE inhibitory effects,and a bioactive molecular network was established to elucidate the ACE inhibitory profile of constituents in FJHQD.This bioactive molecular network provided a panoramic view of FJHQD’s ACE inhibitory activities,which demonstrated that flavones from Astragali Radix and Glycyrrhizae Radix et Rhizoma,saponins from Astragali Radix,and sesquiterpenoids from Atractylodis Macrocephalae Rhizoma were principal components responsible for this effect of FJHQD.Among them,four novel ACE inhibitors were the first to be reported.Our study indicated that the proposed strategy offers a useful approach to uncover the bioactive profile of traditional medicines and provides a pragmatic workflow for exploring bioactive components.展开更多
Until recently, little was known about the fungi found in shark gills and their biomedicinal potential. In this article, we described the isolation, bioactivity, diversity, and secondary metabolites of bioactive fungi...Until recently, little was known about the fungi found in shark gills and their biomedicinal potential. In this article, we described the isolation, bioactivity, diversity, and secondary metabolites of bioactive fungi from the gill of a shark （Carcharodon carcharias）. A total of 115 isolates were obtained and grown in 12 culture media. Fifty-eight of these isolates demonstrated significant activity in four antimicrobial, pesticidal, and cytotoxic bioassay models. Four randomly selected bioactive isolates inhibited human cancer cell proliferation during re-screening. These active isolates were segregated into 6 genera using the internal transcribed spacer-large subunit （ITS-LSU） rDNA-sequence BLAST comparison. Four genera, Penicillium, Aspergillus, Mucor, and Chaetomium were the dominant taxa. A phylogenic tree illustrated their intergenera and intragenera genetic diversity. HPLC-DAD-HRMS analysis and subsequent database searching revealed that nine representative strains produced diverse bioactive compound profiles. These results detail the broad range of bioactive fimgi found in a shark＇s gills, revealing their biopharmaceutical potential. To the best of our knowledge, this is the first study characterizing shark gill fungi and their bioactivity.展开更多
We employed high performance liquid chromatography （HPLC） method coupled with diode array detector （DAD） for the determination of seven major bioactive compounds （forsythoside A, specnuezhenide, phillyrin, schisa...We employed high performance liquid chromatography （HPLC） method coupled with diode array detector （DAD） for the determination of seven major bioactive compounds （forsythoside A, specnuezhenide, phillyrin, schisandrin, schisantherin A, deoxyschizandrin and schisandrin B） in Liuwei Wuling tablet （LWWLT）. The HPLC baseline separation was carried out on an Inertsil ODS-3 column （250 mm×4.6 mm, 5 μm） at 25 ℃ with a gradient elution system composed of 0.1% phosphoric acid solution and acetonitrile. The detection wavelength was set at 230 nm. Calibration curves for seven compounds showed good linear regressions, with correlation coefficients exceeding 0,999 within the tested concentration range. The limits of detection and quantification of each compound were in the range of 0.03-0.28 μg/mL and 0.11-0.87μg/mL, respectively. The relative standard deviation values of precision, stability and repeatability were less than 2.34%. The average recovery of all seven constituents ranged from 96.71% to 103.9%. The validated quantification method was successfully applied for the analysis of 10 batches of commercial LWWLT from Shandong Shibo Jindu Pharmaceutical Co., Ltd. These results would make foundations for quality control and the further pharmacology study of LWWLT.展开更多
Traditional Chinese medicines （TCMs） have a long history of clinical testing and reliable therapeutic efficacy. They have attracted international attention as excellent sources of bioactive compounds for the discove...Traditional Chinese medicines （TCMs） have a long history of clinical testing and reliable therapeutic efficacy. They have attracted international attention as excellent sources of bioactive compounds for the discovery of new drugs. However, TCMs usually contain hundreds or even thousands of components and only a few of these compounds are responsible for the pharmacological effects. The large number of components in TCMs makes the analysis and screening for bioactive compounds extremely difficult. Therefore, strategies for analysis and screening of bioactive compounds in TCMs have long been a focus of studies on TCMs. Cell membrane chromatography （CMC） has become a useful tool for studying the bioactive compounds of TCMs. This review summarizes the principles, characteristics, and application of CMC, and the possible problems faced when working with TCMs. This method will likely become increasingly important for screening and analysis of hioactive compounds in TCMs.展开更多
文摘Spider mites are significant pests in agricultural production.The increasing resistance of spider mites,along with environmental pollution and ecological imbalance caused by their control,is primarily attributed to the long-term use of chemical acaricides in agriculture.In contrast,bioactive substances of biological origin offer advantages such as wide availability,environmental friendliness,and low tendency to induce resistance,making them a research hotspot for spider mite control.This review summarizes recent advances in the use of plant-derived active compounds(exemplified by extracts from Veratrum rhizomes),RNA interference(RNAi)technology,and microorganism-derived active substances for controlling spider mites.These bioactive agents exert acaricidal effects by disrupting the nervous system,interfering with metabolic processes,or silencing key genes in mites,demonstrating favorable efficacy and considerable potential for development.However,challenges remain,including poor environmental stability,slow action,high production costs,and insufficient understanding of their effects on non-target organisms.Therefore,future research should focus on the screening and development of novel bioactive substances of biological origin,elucidation of their mechanisms of action,optimization of formulation technologies,and assessment of their ecological safety.These efforts will provide valuable insights for promoting the advancement of bioactive substances and supporting sustainable agricultural development.
基金Supported by India-South Africa Bilateral Research Grant from the National Research Foundation,Pretoria,South Africa,No.133137.
文摘Obesity and diabetes have become global health crises,with rising prevalence and a strong association with various chronic diseases such as cardiovascular disease,stroke,and certain cancers.These conditions contribute to significant morbidity and mortality,highlighting the urgent need for effective therapeutic interventions.Marine products,including fish oils and marine plants,have been increasingly recognized for their potential in mitigating these diseases.Among these,seaweed stands out due to their diverse bioactive compounds and promising therapeutic effects.This comprehensive review explores the mechanisms of action through which seaweeds,and their compounds exert anti-diabetic and anti-obesity effects,including the regulation of adipogenesis,appetite control,modulation of gut microbiota,enhancement of insulin sensitivity,and reduction of inflammation,oxidative stress,andβ-cell dysfunction.Despite the promising potential,challenges such as variability of bioactive compounds and low bioavailability remain there.Advances in bioactive delivery systems and along with large-scale clinical trials,are crucial for optimizing the therapeutic use of bioactive compounds from seaweeds.Future research should also explore synergistic strategies combining seaweed compounds with other bioactive substances.Overall,seaweed offers a promising foundation for developing functional foods and nutraceuticals aimed at promoting long-term metabolic health,providing an innovative approach to addressing obesity and diabetes.
基金supported by Yildiz Technical University Scientific Research Projects Coordination Unit under project number FBA-2023-5377support from the Scientific and Technological Research Council of Turkey(TUBITAK)under the BIDEB/2211-A National PhD Scholarship Program and 2250-Performance-Based Scholarships Program for PhD.
文摘In this study,injectable bone graft putty samples were developed using fine and coarse melt-quenched 45S5 bioactive glass(BG)incorporated into a carrier system composed of glycerol and polyethylene glycol(PEG)with different average molecular weights.Selected putty samples were further incorporated with varying amounts of Denosumab(5wt%-10wt%)to investigate its influence on rhe-ological behavior and flow properties using mathematical modeling.All PEG/glycerol/45S5-based putty samples exhibited viscoelastic behavior(storage modulus>loss modulus)and pseudoplastic behavior(n<1),with viscosity values required for optimal flow remaining below 1000 Pa∙s.Both viscosity and thixotropic area increased proportionally with higher BG content and smaller-sized BG particles.All putty samples showed more than 98%injectability through a 12G cannula,suggesting potential clinical suitability.However,injectability decreased with smaller cannulas,dropping to 34.7%-58.3%with a 19G cannula and further decreasing with a 23G cannula at higher BG contents.Incorporation of Denosumab preserved viscoelasticity and injectability but modified the flow behavior,shifting it from pseudo-plastic to more Newtonian with higher Denosumab content,while also reducing viscosity and thixotropic area values.Among all tested samples,putty containing a lower amount of Denosumab and smaller-sized BG exhibited the most suitable combination of injectability and rheological features.All putty samples were well described by both the Power law and Herschel-Bulkley rheological models(coeffi-cient of determination>0.95).This study highlights the influence of Denosumab on flowability and rheological relationships and sug-gests potential improvements in bioactivity through a dual synergistic effect of BG and Denosumab in minimally invasive bone graft sys-tems.
基金supported by Korea Institute of Energy Technology Evaluation and Planning(KETEP)grant funded by the Korea government(MOTIE)(20212020800050,Development and demonstration of rooftop greenhouse-building integrated system using distributed polygeneration).
文摘Agastache rugosa,a medicinal plant known for its bioactive compounds,has gained attention for its pharmacological and commercial potential.This study aimed to optimize ethanol concentration to enhance growth and bioactive compound production in A.rugosa cultivated in a controlled plant factory system.Ethanol treatments at 40 and 80 mM significantly promoted both vegetative and reproductive growth.Plants treated with these concentrations exhibited higher net photosynthetic rates(A)and intercellular CO_(2) concentration(Ci)compared to the untreated control,whereas stomatal conductance(gs)and transpiration rate(E)remained unaffected.Chlorophyll and carotenoid concentrations,and SPAD values,significantly increased with ethanol treatment.Total flavonoid and total phenolic contents as well as 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical-scavenging activities were significantly higher in plants treated with ethanol than in the untreated control.Ethanol treatments led to a significant enhancement in the activities of antioxidant enzymes,including superoxide dismutase,peroxidase,and catalase.Furthermore,ethanol treatment elevated rosmarinic acid concentrations in roots and tilianin and acacetin levels in flowers.Collectively,ethanol at 40 and 80 mM effectively enhanced growth,photosynthesis,antioxidant defense,and bioactive compound production in A.rugosa cultivated in a plant factory.These findings provide valuable insights for improving cultivation of medicinal plants with high pharmaceutical and nutraceutical value.
基金supported by Shaanxi Province Qin Chuangyuan“Scientist+Engineer”team construction project(2024QCY-KXJ-079)Shaanxi Science and Technology Innovation Team Project(2024RSCXTD-70)for the financial support。
文摘Roasting is important for improving the content of bioactive compounds and enhancing the flavor of flaxseed oil.However,the effect of roasting time on germinated flaxseed oil(GFO)is unclear.Herein,the influence of roasting time(0–50 min)at a specific temperature of 160?C on the physicochemical properties,bioactive compounds,antioxidant capacity,and volatile compounds of GFO from three flaxseed varieties was examined.An extension in roasting time increased the a^(*)value of GFO,while the L^(*)and b^(*)values of GFO decreased.Higher chlorophylls(1.63±0.01–2.18±0.01 mg/kg),carotenoids(2.25±0.00–2.69±0.02 mg/kg),total phenolic content(156.75±2.93–200.03±6.29 mg GAE/kg),phytosterol(1801.93±30.64–1965.23±13.71 mg GAE/kg)and DPPH(320.11±18.22–432.19±14.95μmol TE/kg)and ABTS radical scavenging activity(810.68±16.29–1119.01±14.25μmol TE/kg)were detected in GFO after roasting for 20–30 min.However,the total tocopherol and unsaturated fatty acid content declined,and the composition of fatty acids changed insignificantly(P>0.05).GC–MS recognized 68 volatile compounds in GFO,in which pyrazine imparted a nutty flavor to the GFO.This study provides important information for the development of high-quality GFO.
文摘The cosmetic sector is a multibillion-dollar industry that requires constant attention being paid to innovative product development and engagement.Notably,its market value is projected to exceed 750 billion U.S.dollars by 2025,and it is expanding as novel,climate-friendly,green,and sustainable components from natural sources are incorporated.This review is written based on the numerous reports on the potential applications of food-derived peptides while focusing on their possible uses in the formulation of cosmeceutical and skincare products.First,the production methods of bioactive peptides linked to cosmeceutical uses are described.Then,we discuss the obtainment and characterization of different anti-inflammatory,antimicrobial,antioxidant,anti-aging,and other pleiotropic peptides with their specific mechanisms,from various food sources.The review concludes with salient considerations of the cost of production and pilot scale operation,stability,compatibility,user safety,site-specificity,and delivery methods,when designing or developing biopeptide-based cosmeceutical products.
基金support from Qinghai Province basic research project(2024-ZJ-760)Changzhou Sci&Tech Program(CZ20240029)+1 种基金Science and Technology Project of Changzhou Health Commission(QN202356)Top Talent of Changzhou“The 14th Five-Year Plan”High-Level Health Talents Training Project(2022CZBJ059 and 2022CZBJ061).
文摘Introduction The global burden of orthopedic diseases has reached unprecedented levels,with recent epidemiological data revealing that musculoskeletal conditions affect over 1.71 billion people worldwide,representing a 150%increase since 1990[1].By 2050,the number of individuals aged 60 and above requiring orthopedic interventions is projected to exceed 2.1 billion,with osteoporosis alone affecting 200 million people globally.
基金supported by the U.S.Department of Agriculture(USDA),National Institute of Food and Agriculture,Award No.2022-67018-37143.
文摘In this study,hydrophobically modified phytoglycogen and hydrolyzed zein were utilized to develop biodegradable and redispersible nanocomplexes for encapsulation of various bioactive phytochemicals,including eugenol,thymol,carvacrol,trans-cinnamaldehyde,and their mixtures.Comprehensive physicochemical and colloidal characterizations of these nanocomplexes were performed using dynamic light scattering,and Fourier transform infrared and atomic force microscopy,which evaluated their colloidal stability and redispersibility after freeze-drying.In addition,the antioxidant capacities and antimicrobial activities of these various nanoparticles were investigated methodically.The results revealed that the phytochemical-encapsulated nanocomplexes exhibited a particle size ranging from 60 to 70 nm,display-ing a uniform distribution.Additionally,these nanocomplexes demonstrated sustained morphological and colloidal stability over 30-day storage,and after freeze-drying and subsequent redispersion processing.Furthermore,significant improvements in antioxidant and antimicrobial properties in the aqueous phase were observed.These redispersible nanocomplexes from phytoglycogen and zein hold the promising potential to address the limitations and offer practical solutions for expanding their applications in the pharmaceutical and food fields.
基金The financial support received from the Shenzhen Science and Technology Innovation Commission(KCXFZ20201221173207022,WDZC20200821141349001)Shenzhen Bay Laboratory Startup Fund(21310041,S234602003)。
文摘Pu-erh tea,a traditional Chinese beverage,performs an anti-obesity function,but the correlation between its components and efficacy remains unknown.Here,we screened two Pu-erh teas with significant anti-obesity efficacies from 11 teas.In vitro experiments revealed that lipid accumulation in L02 cells and lipid synthesis in 3T3-L1 cells were significantly better inhibited by Tea-B than Tea-A.Further in vivo experiments using model mice revealed that the differences in chemical components generated two pathways in the anti-obesity efficacy and mechanism of Pu-erh teas.Tea-A changes the histomorphology of brown adipose tissue(BAT)and increases the abundance of Coriobacteriaceae_UCG_002 and cyclic AMP in guts through high chemical contents of cyclopentasiloxane,decamethyl,tridecane and 1,2,3-trimethoxybenzene,eventually increasing BAT activation and fat browning gene expression;the high content of hexadecane and 1,2-dimethoxybenzene in Tea-B reduces white adipose tissue(WAT)accumulation and the process of fatty liver,increases the abundance of Odoribacter and sphinganine 1-phosphate,inhibits the expression of lipid synthesis and transport genes.These mechanistic findings on the association of the representative bioactive components in Pu-erh teas with the anti-obesity phenotypes,gut microbes,gut metabolite structure and anti-obesity pathways,which were obtained for the first time,provide foundations for developing functional Pu-erh tea.
基金financially supported by the National Natural Science Foundation of China(No.52122308)the Joint Fund of Science and Technology R&D Plan of Henan Province(No.232301420042)
文摘Bacterial infections have seriously impaired the wound healing.Despite numerous antibiotics have been developed to inhibit bacteria,the emergence of antibiotic resistance necessitates a rethinking of the public health crisis.In this study,we introduce the carbon dots derived from metformin and dopamine(MD-CDs)as a novel approach to promote the healing of bacterial-infected wound.Through a hydrothermal reaction,the regularly spherical MD-CDs were prepared.Owing to their inherent antibacterial property,MD-CDs effectively inhibited the wound infection and expedited the transition from the inflammation phase to the healing phase in infected wound.Additionally,MD-CDs enhanced the proliferation and migration of L929 cells,which is crucial for wound tissue regeneration.Following the treatment of infected fullthickness skin wounds,MD-CDs effectively promoted the collagen deposition and regeneration of hair follicle in the healing phase.Consequently,MD-CDs represent a bioactive nanomaterial exhibiting inherent antibacterial,offering a promising alternative for the treatment of infected wounds.
基金supported by the Natural Science Foundation of Shandong Province(No.ZR2023ZD25)the Shandong Province Chinese Medicine Science and Technology Development Project(No.Q-2023107)the Taishan Scholars Project in Shandong Province(Nos.tstp202306 and tsqn202408246).
文摘The carbonization processing(Paozhi)in traditional Chinese medicine(TCM)represents a unique pharmaceutical technology where thermal modification of herbal materials enhances specific therapeutic properties,particularly hemostatic and antioxidant effects.Despite centuries of empirical applications,the scientific basis underlying these enhanced bioactivities remains poorly characterized,particularly regarding the transformation and functionalization of active components during high-temperature carbonization.This study systematically investigates carbon dots(CDs),emerging carbon-based nanomaterials spontaneously formed during the carbonization process,as potential key bioactive constituents mediating the therapeutic actions of carbonized TCM.Through multidisciplinary analysis of pyrolysis-driven CD formation mechanisms,nanostructural evolution,and surface chemistry modulation,we demonstrate that CDs exhibit size-dependent fluorescence properties and redox-active surface functional groups that correlate with their observed biological effects.Crucially,the study establishes quantitative structure-activity relationships between CDs’quantum confinement characteristics(2–8 nm diameter),oxygencontaining surface moieties(carboxyl,hydroxyl groups),and their procoagulant/antioxidant capacities.By bridging traditional processing knowledge with nanotechnology insights,this work not only deciphers the“black box”of thermal processing in TCM but also proposes a nano-biointerface paradigm for understanding Paozhi mechanisms.The findings advance quality control strategies through CD-based spectral fingerprinting and open new avenues for developing nanoscale TCM derivatives with optimized therapeutic profiles.
基金funded by National Key R&D Program of China(2022YFF1100301,2023YFF1104403)Key R&D Project of Henan Province(231111112100)。
文摘Angelica sinensis,a well-known traditional Chinese medicinal herb with the unique property of being both a medicine and an edible plant,has been widely used for promoting blood circulation,modulating immunity,and relieving pain.This review comprehensively investigates the extraction methods,structural characteristics,and biological activities of its primary bioactive components,such as polysaccharides,volatile oils,organic acids,and flavonoids.The biosynthesis pathways of these compounds,along with the key enzymes and transcription factors involved,are investigated to understand the factors influencing their synthesis and accumulation.Additionally,the biological activities of A.sinensis,including hepatoprotective,anti-inflammatory,immunemodulatory,anti-tumor,circulatory benefits,and neuroprotection,along with their underlying mechanisms are introduced.These findings provide a solid foundation for the development of A.sinensis as a valuable resource in functional foods and pharmaceutical products.
基金supported by the National Natural Science Foundation of China(82372405,81871752)the Key Research and Development Program of Hubei Province(2022BCA052)+2 种基金the Key Research and Development Program of Wuhan City(2024020702030105)the Fundamental Research Funds for the Central Universities(2042023kf0199)the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University(ZNJC202014).
文摘Bioactive peptides and proteins(BAPPs)are promising therapeutic agents for tissue repair with considerable advantages,including multifunctionality,specificity,biocompatibility,and biodegradability.However,the high complexity of tissue microenvironments and their inherent deficiencies such as short half-live and susceptibility to enzymatic degradation,adversely affect their therapeutic efficacy and clinical applications.Investigating the fundamental mechanisms by which BAPPs modulate the microenvironment and developing rational delivery strategies are essential for optimizing their administration in distinct tissue repairs and facilitating clinical translation.This review initially focuses on the mechanisms through which BAPPs influence the microenvironment for tissue repair via reactive oxygen species,blood and lymphatic vessels,immune cells,and repair cells.Then,a variety of delivery platforms,including scaffolds and hydrogels,electrospun fibers,surface coatings,assisted particles,nanotubes,two-dimensional nanomaterials,and nanoparticles engineered cells,are summarized to incorporate BAPPs for effective tissue repair,modification strategies aimed at enhancing loading efficiencies and release kinetics are also reviewed.Additionally,the delivery of BAPPs can be precisely regulated by endogenous stimuli(glucose,reactive oxygen species,enzymes,pH)or exogenous stimuli(ultrasound,heat,light,magnetic field,and electric field)to achieve on-demand release tailored for specific tissue repair needs.Furthermore,this review focuses on the clinical potential of BAPPs in facilitating tissue repair across various types,including bone,cartilage,intervertebral discs,muscle,tendons,periodontal tissues,skin,myocardium,nervous system(encompassing brain,spinal cord,and peripheral nerve),endometrium,as well as ear and ocular tissue.Finally,current challenges and prospects are discussed.
基金the funds from the National Key R&D Program of China(2022YFF1100200).
文摘Oxidative stress is caused by an imbalance of the antioxidant defense system and excessive production of free radicals,which can damage biological macromolecules such as proteins,lipids and nucleic acids,and can cause aging,ischemia-reperfusion injury,inflammatory injury,liver and kidney injury and other diseases.The nuclear factor erytheroid-2-related factor 2(Nrf2)is a major regulator of redox balance.The Nrf2 pathway also exerts a central function in cell apoptosis,oxidative stress damage and metabolic diseases.Bioactive peptides are small molecular peptides composed of amino acid residues.They have many biological activities and play important physiological roles in human body.Antioxidant peptide is a kind of peptide which can reduce oxidative stress damage.They are safe,non-toxic and easily absorbed.In this review,we summarized the mechanism of bioactive peptides in regulating oxidative stress,especially antioxidant peptides,through regulating the Nrf2 signaling pathway and the expressions of oxidative stress-related genes,to alleviate oxidative stress-induced damage.The paper will provide valuable reference to investigators in the antioxidant peptide field and also promote the applications of antioxidant peptides in the oxidative stress-associated diseases.
基金supported by National Natural Science Foundation of China(Grant 52277231)City University of Hong Kong Donation Research(Grant DON-RMG 9229021)Hong Kong PDFS—RGC Postdoctoral Fellowship Scheme(PDFS2122-1S08).
文摘Cold atmospheric plasmas are widely used in biomedicine.Although direct plasma treatments of wounds have been demon-strated,there are still obstacles hampering further clinical adoption,for example,the limited treatment area,inconsistent ac-tions and risk of thermal injury.In this respect,plasma-activated air(PAA)is proposed and demonstrated for infected wounds treatment as an alternative to the conventional direct plasma treatment.The combination of gliding arc discharge reactor and dielectric barrier discharge reactor produces highly bioactive PAA.In in vitro sterilisation of Staphylococcus aureus,approxi-mately 9-log reduction is achieved after the PAA treatment for 6 min.Bovine serum albumin is added to the S.aureus sus-pension to further simulate the wound exudate to accomplish inactivation of approximately 3-log reduction after 10 min.In vivo experiments show that the PAA treatment of infected wounds significantly reduces the bacterial load and improves the healing rate,revealing an optimal treatment time of 3 min/day.The immunohistochemical and blood biochemical analyses show that the PAA-3 min treatment enhances wound healing by inhibiting the tissue inflammatory response and inducing growth factor production without showing evident systemic toxicity.In conclusion,PAA holds great clinical promise as a safe and effective wound-healing strategy.
基金supported by the National Natural Science Foundation of China(22408078,82401057,32101170)the Zhejiang Province Postdoctoral Excellence Funding Program-Special Support(ZJ2024004).
文摘Diabetic wounds(DWs)are a major complication of diabetes mellitus,characterized by a complex patho-physiological microenvironment that is associated with elevated morbidity and mortality.Conventional management strategies often fail to address the multifaceted nature of these wounds effectively.Recent advancements in understanding the mechanisms of DW healing have spurred the development of a plethora of bioactive dressings designed to interact with and modulate the DW microenvironment.These innovations have culminated in the introduction of the“microenvironment-sensitive with on-demand management”paradigm aimed at delivering precision therapy responsive to dynamic changes within DW.Despite these advancements,the current literature lacks a comprehensive review that cate-gorizes and evaluates active,passive,and on-demand approaches that address the DW microenviron-ment.Herein,we describe the unique pathogenic mechanisms and microenvironmental characteristics that distinguish DW from normal acute wounds.This review provides an extensive overview of contem-porary active and passive management strategies incorporating on-demand management principles designed for DW microenvironments.Furthermore,it addresses the principal challenges faced in this therapeutic domain and outlines the potential innovations that can enhance the efficacy and specificity of bioactive dressings.The insights presented here aim to guide further research and development in the on-demand management of DW to improve patient outcomes by aligning personalized therapy modali-ties with the pathophysiological realities of DW.
基金financially supported by the National Key R&D Program of China(Grant No.:2018YFC1704502)the National Natural Science Foundation of China(Grant No.:81603268)the National Natural Science Foundation of China(Grant No.:81822047)
文摘Natural products are great treasure troves for the discovery of bioactive components.Current bioassay guided fractionation for identification of bioactive components is time-and workload-consuming.In this study,we proposed a robust and convenient strategy for deciphering the bioactive profile of natural products by mass spectral molecular networking combined with rapid bioassay.As a proof-of-concept,the strategy was applied to identify angiotensin converting enzyme(ACE)inhibitors of Fangjihuangqi decoction(FJHQD),a traditional medicine clinically used for the treatment of heart failure.The chemical profile of FJHQD was comprehensively revealed with the assistance of tandem mass spectral molecular networking,and a total of 165 compounds were identified.With characterized constituents,potential clinical applications of FJHQD were predicted by Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine,and a range of cardiovascular related diseases were significantly enriched.ACE inhibitory activities of FJHQD and its constituents were then investigated with an aggregation-induced emission based fluorescent probe.FJHQD exhibited excellent ACE inhibitory effects,and a bioactive molecular network was established to elucidate the ACE inhibitory profile of constituents in FJHQD.This bioactive molecular network provided a panoramic view of FJHQD’s ACE inhibitory activities,which demonstrated that flavones from Astragali Radix and Glycyrrhizae Radix et Rhizoma,saponins from Astragali Radix,and sesquiterpenoids from Atractylodis Macrocephalae Rhizoma were principal components responsible for this effect of FJHQD.Among them,four novel ACE inhibitors were the first to be reported.Our study indicated that the proposed strategy offers a useful approach to uncover the bioactive profile of traditional medicines and provides a pragmatic workflow for exploring bioactive components.
基金Supported by the National Natural Science Foundation of China(No.20902009)the National Science Foundation for Post-Doctoral Scientists of China(Nos.2011M500051,2012T50258)+2 种基金the Yang Fan Scarce Top Talent Project of Guangdong Province(to ZHANG Yi)the Program for Scientific Research Start-up Funds of Guangdong Ocean University(GDOU)(to ZHANG Yi)the Natural Science Research Project of GDOU(No.C14519)
文摘Until recently, little was known about the fungi found in shark gills and their biomedicinal potential. In this article, we described the isolation, bioactivity, diversity, and secondary metabolites of bioactive fungi from the gill of a shark （Carcharodon carcharias）. A total of 115 isolates were obtained and grown in 12 culture media. Fifty-eight of these isolates demonstrated significant activity in four antimicrobial, pesticidal, and cytotoxic bioassay models. Four randomly selected bioactive isolates inhibited human cancer cell proliferation during re-screening. These active isolates were segregated into 6 genera using the internal transcribed spacer-large subunit （ITS-LSU） rDNA-sequence BLAST comparison. Four genera, Penicillium, Aspergillus, Mucor, and Chaetomium were the dominant taxa. A phylogenic tree illustrated their intergenera and intragenera genetic diversity. HPLC-DAD-HRMS analysis and subsequent database searching revealed that nine representative strains produced diverse bioactive compound profiles. These results detail the broad range of bioactive fimgi found in a shark＇s gills, revealing their biopharmaceutical potential. To the best of our knowledge, this is the first study characterizing shark gill fungi and their bioactivity.
基金State Project for Essential Drug Research and Development(Grant No.2012ZX09J12108-04C)Special Project of Military Health Ministry(Grant No.13ZJZ12-5)
文摘We employed high performance liquid chromatography （HPLC） method coupled with diode array detector （DAD） for the determination of seven major bioactive compounds （forsythoside A, specnuezhenide, phillyrin, schisandrin, schisantherin A, deoxyschizandrin and schisandrin B） in Liuwei Wuling tablet （LWWLT）. The HPLC baseline separation was carried out on an Inertsil ODS-3 column （250 mm×4.6 mm, 5 μm） at 25 ℃ with a gradient elution system composed of 0.1% phosphoric acid solution and acetonitrile. The detection wavelength was set at 230 nm. Calibration curves for seven compounds showed good linear regressions, with correlation coefficients exceeding 0,999 within the tested concentration range. The limits of detection and quantification of each compound were in the range of 0.03-0.28 μg/mL and 0.11-0.87μg/mL, respectively. The relative standard deviation values of precision, stability and repeatability were less than 2.34%. The average recovery of all seven constituents ranged from 96.71% to 103.9%. The validated quantification method was successfully applied for the analysis of 10 batches of commercial LWWLT from Shandong Shibo Jindu Pharmaceutical Co., Ltd. These results would make foundations for quality control and the further pharmacology study of LWWLT.
基金Scientific and Technological Innovation Project Foundation of Shanxi China (Grant No. 20090321099)
文摘Traditional Chinese medicines （TCMs） have a long history of clinical testing and reliable therapeutic efficacy. They have attracted international attention as excellent sources of bioactive compounds for the discovery of new drugs. However, TCMs usually contain hundreds or even thousands of components and only a few of these compounds are responsible for the pharmacological effects. The large number of components in TCMs makes the analysis and screening for bioactive compounds extremely difficult. Therefore, strategies for analysis and screening of bioactive compounds in TCMs have long been a focus of studies on TCMs. Cell membrane chromatography （CMC） has become a useful tool for studying the bioactive compounds of TCMs. This review summarizes the principles, characteristics, and application of CMC, and the possible problems faced when working with TCMs. This method will likely become increasingly important for screening and analysis of hioactive compounds in TCMs.
