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Rhizobium-Based Biofertilizer for Rational Use of Mineral Nitrogen Fertilizers: Case Study of Common Bean Cultivation in Senegal
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作者 Adama Diouf 《American Journal of Plant Sciences》 2025年第1期133-145,共13页
Synthetic fertilizers are widely used to address the urgent challenge of ensuring food supplies for a growing world population in the context of climate change. However, their industrial production and use in agricult... Synthetic fertilizers are widely used to address the urgent challenge of ensuring food supplies for a growing world population in the context of climate change. However, their industrial production and use in agriculture have a negative impact on the environment and consequently on human health. While chemical fertilizers may not have to be abandoned in agricultural production systems, limiting their use could help to make agriculture sustainable and resilient to climate change. In Senegal, the level of mineral fertilizers used in market gardening has become alarming in the Niayes area. As a result, microbial biotechnologies have been promoted for biofertilizer production of common bean (Phaseolus vulgaris L.) cultivation. Rhizobial inoculums have thus been used to reduce the rate of chemical nitrogen fertilizers being applied in cropping systems. Several investigations in the laboratory, on experimental stations and in the field have shown a possibility of a significant reduction in the use of nitrogen fertilizers in common bean production. Conventional mineral fertilization use can be reduced from over 120 kg N/ha to 20 kg N/ha. This contributes both to a very significant reduction in the application rate with the same level of yield and to an improvement in the standard of living. In addition, the environmental impact of using chemical fertilizers can be mitigated. This study is a contribution to the promotion of biofertilizers adoption in agricultural systems. 展开更多
关键词 Sustainable Agriculture Mineral Fertilizer Inoculation RHIZOBIUM Common bean
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Influence of Fermentation and Drying Practices on the Ochratoxin A Content of Cocoa Beans from the Main Production Areas in Côte d’Ivoire
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作者 Brou Julien Kouakou Kouadio Emmanuel N’goran Koffi Christophe Kobenan 《Advances in Microbiology》 2025年第1期70-79,共10页
Côte d’Ivoire has been the world’s leading producer of cocoa beans for several decades. Apart from this production performance, the quality of the beans, which are mainly exported to the major chocolate-making ... Côte d’Ivoire has been the world’s leading producer of cocoa beans for several decades. Apart from this production performance, the quality of the beans, which are mainly exported to the major chocolate-making countries, presents a quality problem to the point of suffering a discount on the international market. One of these quality problems is the content of ochratoxin A, a mycotoxin produced by fungi. Finally, to verify the level of contamination in beans produced in Côte d’Ivoire, a study was carried out. It consisted of collecting information on fermentation and drying times (The two major post-harvest operations) and collecting beans, which were analyzed by electrophoresis using the High Performance Liquid Chromatography (HPLC) method. The results obtained show ochratoxin A contents of between 0.05 µg/kg and 0.17 µg/kg. The general level of contamination is therefore very low and below the tolerable limit which is 2 µg/kg. In addition, the correlative study between the fermentation and drying times of the beans revealed no significant influence (p < 0.01) of the duration of these operations on the level of ochratoxin A contamination. Major contamination can occur after post-harvest activities carried out by producers. This is certainly due to the development of fungi responsible for the production of ochratoxin A during the period of storage and marketing of cocoa beans in conditions of high humidity in storage enclosures. Producers need to be made more aware of the need to ensure that cocoa beans are properly dried and stored in dry areas to avoid moisture build-up, which is a source of mould growth and ochratoxin A production. 展开更多
关键词 Ochratoxin A Cocoa beans FERMENTATION Drying Practices Cote d’Ivoire Mycotoxins HPLC Analysis Fungal Contamination Post-Harvest Operations Food Safety
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Hesperidin encapsulation in mung bean isolate protein-dextran conjugate-stabilized nanoemulsions:Preparation and characterization
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作者 Zixi Xue Xianrong Xiang +3 位作者 Jiaying Tang Wen Qin Guohua Zhang Qing Zhang 《Grain & Oil Science and Technology》 2025年第4期247-260,共14页
Encapsulation and protection of hesperidin(HES)in mung bean protein isolate(MPI)-dextran(DX)conjugatestabilized nanoemulsions(MDC NEs)were investigated in this study.The degree of grafting of MDC prepared by a dry-hea... Encapsulation and protection of hesperidin(HES)in mung bean protein isolate(MPI)-dextran(DX)conjugatestabilized nanoemulsions(MDC NEs)were investigated in this study.The degree of grafting of MDC prepared by a dry-heating method reached 39.70%±0.01% under the optimal conditions of MPI/DX mass ratio 1:2.3,reaction temperature 58.8℃,and reaction time 4 d.Moreover,the analyses of Fourier infrared spectroscopy,intrinsic fluorescence spectroscopy,surface hydrophobicity,and thermal stability further confirmed the covalent grafting of dextran onto MPI molecules.When encapsulated in MDC NEs at 80 MPa for three times by highpressure homogenization,the encapsulation efficiency and loading capacity of HES were 63.62%±0.01%and 0.40±0.00 g/g,respectively.The encapsulated HES exhibited higher antioxidant activity and stronger light and storage stability than the free HES.Additionally,the incorporation of HES inhibited the formation of lipid peroxides in the nanoemulsions.The findings suggest that glycosylation combined with high-pressure homogenization is an effective strategy for enhancing the stability of MPI-based emulsions and improving their encapsulation of HES.This study provides a promising approach for the development of innovative food and beverage products based on MPI emulsions or new materials for encapsulating fat-soluble bioactive compounds. 展开更多
关键词 Mung bean protein isolate-dextran conjugate NANOEMULSIONS Encapsulation HESPERIDIN Stability
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Fluctuating ozone exposures caused trade-offs between vegetative growth and reproduction of two Chinese bean cultivars and ethylenediurea alleviated ozone phytotoxicities
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作者 Kun Zhang Le Xian +3 位作者 Bo Shang Yansen Xu Zhaozhong Feng Evgenios Agathokleous 《Journal of Environmental Sciences》 2025年第10期450-465,共16页
Ozone(O_(3))pollution has elevated in China,threatening plants and crop production.Ethylenediurea(EDU)is a chemical alleviating O_(3)-induced phytotoxicities.This study aimed at revealing fluctuating O_(3) exposures e... Ozone(O_(3))pollution has elevated in China,threatening plants and crop production.Ethylenediurea(EDU)is a chemical alleviating O_(3)-induced phytotoxicities.This study aimed at revealing fluctuating O_(3) exposures effects on Youxian No 3(Phaseolus vulgaris)and Sukui No 4(Vigna angularis),two widely grown Chinese bean cultivars,and EDU role in mediating these effects.Plants were periodically treated with EDU(400 mg/L)or water and subjected to charcoal-filtered air(CF)or non-filtered ambient air enriched with an additional targeted O_(3) concentration of 40 ppb(NF40).with subsequent ambient or NF40 exposures.A 10-day exposure to NF40 increased photosynthetic rate(A)while decreasing the leaf intercellular CO_(2) concentration(Ci),but this effect was absent after moving plants to ambient air for two weeks.Moving previously CF-exposed plants to ambient air for two weeks also increased A,which was not linked with Ci but more related to stomatal conductance(gs).Following two one-week and two-week sequential exposures of all plants to NF40,with an intermediate exposure to ambient air,elevated O_(3) reduced chlorophylls(SPAD),A,gs,Ci,and transpiration and decoupled A-gs response.More O_(3) effectswere observed in plants treatedwithNF40 during each O_(3)-treatment cycle,compared to those exposed to CF during the first cycle.The former plants exhibited significantly decreased biomass and water content of leaves and stems but increased flowers biomass and water content.Some of the effects were cultivardependent,with Youxian showing more apparent trade-offs between vegetative growth and reproduction.EDU alleviated various negative O_(3) effects. 展开更多
关键词 Antiozonant bean cultivation Crop protection Ozone pollution Plant oxidative stress
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Determination of Ten Plant Growth Regulator Residues in Bean Sprouts by Mass Spectrometry
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作者 Lin TIAN Jinlu LI +6 位作者 Yi ZHANG Aili XIANG Baiqin ZHENG Zhe YIN Dan WU Caihua ZHANG Lei WANG 《Agricultural Biotechnology》 2025年第3期6-8,16,共4页
[Objectives]This study was conducted to quickly qualitatively and quantitatively analyze the residues of 10 plant growth regulators(PGRs)in bean sprouts.[Methods]Using bean sprouts as the test material,a high-performa... [Objectives]This study was conducted to quickly qualitatively and quantitatively analyze the residues of 10 plant growth regulators(PGRs)in bean sprouts.[Methods]Using bean sprouts as the test material,a high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was established to determine the residual levels of 10 PGRs in bean sprouts.[Results]Under optimized conditions,the retention time of the 10 PGRs ranged from 6.45 to 11.43 min.When the mass concentration ranged from 0.005 to 0.05μg/ml,all PGRs exhibited good linearity,with correlation coefficients(r)≥0.9991.The limits of detection(LODs,S/N=3)were in the range of 0.30-0.92μg/kg,and the limits of quantification(LOQs)were in the range of 0.50-2.10μg/kg.The average recovery values at three concentration levels ranged from 80%to 105.8%,with relative standard deviations(RSD s,n=6)of 2.8%-7.5%.[Conclusions]This method is simple and accurate,and provides technical reference for food safety monitoring. 展开更多
关键词 HPLC-MS/MS bean sprout Plant growth regulators Mass spectrometry
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Effects of Acupoint Catgut Embedding Combined with Auricular Point Pressing with Beans on Self- Efficacy of Symptom Management and Quality of Life of Patients with Nonalcoholic Steatohepatitis of Liver Depression and Spleen Deficiency Type
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作者 Jiamin Feng Zhentong Xia +1 位作者 Lifen Wu Fangyao Zhao 《Journal of Clinical and Nursing Research》 2025年第5期350-358,共9页
Objective:To explore the effects of acupoint catgut embedding combined with auricular point pressing with beans on symptom management self-efficacy and quality of life in patients with nonalcoholic steatohepatitis(NAS... Objective:To explore the effects of acupoint catgut embedding combined with auricular point pressing with beans on symptom management self-efficacy and quality of life in patients with nonalcoholic steatohepatitis(NASH)of liver depression and spleen deficiency type.Methods:Sixty patients with NASH of liver depression and spleen deficiency type admitted to our hospital from January 2021 to December 2023 were selected and divided into an acupoint catgut embedding group(n=30)and a combined group(n=30)using the envelope lottery method.The acupoint catgut embedding group received acupoint catgut embedding intervention,while the combined group received auricular point pressing with beans on the basis of the acupoint catgut embedding group.The two groups were compared in terms of TCM syndrome scores,symptom management self-efficacy[Chronic Disease Self-Efficacy Scale(CDSES)],and quality of life[Chronic Liver Disease Questionnaire(CLDQ)].Results:After intervention,the combined group had lower TCM syndrome scores for both primary and secondary symptoms compared to the acupoint catgut embedding group(P<0.05).The combined group also had higher scores in all dimensions and total score of the CDSES compared to the acupoint catgut embedding group(P<0.05).Similarly,the combined group had higher scores in all dimensions and total score of the CLDQ compared to the acupoint catgut embedding group(P<0.05).Conclusion:Acupoint catgut embedding combined with auricular point pressing with beans can effectively improve TCM symptoms,enhance symptom management self-efficacy,and improve quality of life in patients with NASH of liver depression and spleen deficiency type. 展开更多
关键词 Nonalcoholic steatohepatitis Liver depression and spleen deficiency Acupoint catgut embedding Auricular point pressing with beans Symptom management SELF-EFFICACY Quality of life
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Gap-free genome assembly and metabolomics analysis of common bean provide insights into genomic characteristics and metabolic determinants of seed coat pigmentation
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作者 Bo Zhao Hui Zhang +13 位作者 Qin Zhao Rina Wu Qian You Bihui Wang Yiming Wang Zhilan Yan Peng Wang Chunguo Huang Wei Zhang Youmei Wang Xiaopeng Hao Jing Wu Lixiang Wang Zhaosheng Kong 《Journal of Genetics and Genomics》 2025年第6期852-855,共4页
Common bean(Phaseolus vulgaris L.)is a vital source of protein and essential nutrients for human consumption and plays a key role in sustainable agriculture due to its nitrogen-fixing ability(Nadeem et al.,2021).Kidne... Common bean(Phaseolus vulgaris L.)is a vital source of protein and essential nutrients for human consumption and plays a key role in sustainable agriculture due to its nitrogen-fixing ability(Nadeem et al.,2021).Kidney beans,a subcategory of dry common beans,are highly valued for their rich protein,dietary fiber,low fat content,and various trace elements(Garcia-Cordero et al.,2021).Despite the release of several de novo genome assemblies(Goodstein et al.,2012;Schmutz et al.,2014;Vlasova et al.,2016;Cortinovis et al.,2024),existing common bean genomes remain incomplete,particularly in complex regions such as centromeres and telomeres,limiting a comprehensive understanding of the genomic landscape. 展开更多
关键词 trace elements garcia cordero genomic characteristics gap free genome assembly metabolomics analysis common beansare de novo genome assemblies goodstein sustainable agriculture common bean
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蚕豆11S蛋白淀粉样纤维化聚集的结构表征及其功能特性研究
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作者 陈慧 周梦园 +5 位作者 许喆 林学镁 赵文忠 许志豪 丁笑琦 丁玉庭 《食品与发酵工业》 北大核心 2026年第1期277-283,共7页
食源性蛋白淀粉样纤维化聚集具有独特的结构特性,蚕豆11S蛋白(fava bean 11S protein,FP)作为一种可持续蛋白资源,表现出巨大的潜力。该研究探究了蚕豆11S蛋白淀粉样纤维化聚集(fibrotic aggregation of 11S protein in fava bean,FPF)... 食源性蛋白淀粉样纤维化聚集具有独特的结构特性,蚕豆11S蛋白(fava bean 11S protein,FP)作为一种可持续蛋白资源,表现出巨大的潜力。该研究探究了蚕豆11S蛋白淀粉样纤维化聚集(fibrotic aggregation of 11S protein in fava bean,FPF)在形成过程中的动态演变,包括其结构表征和功能特性。6 g/100 mL的FP通过酸热处理(pH 2,85℃)不同时间(0~24 h)后得到FPF。处理后的样品通过硫黄素T、荧光、二酪氨酸、透射电子显微镜、傅里叶红外光谱等进行结构表征,结果表明FP先在酸热过程中水解成多肽,再自组装成富含β-折叠结构的FPF(由0 h的34.44%增加到24 h的45.89%)。通过起泡性、乳化性和凝胶特性等对FPF功能特性进行表征,与FP相比,反应24 h后的FPF具有更好的起泡性、乳化性和凝胶特性。此外,FPF在体外细胞实验中没有表现出细胞毒性。研究结果为FPF的形成规律提供了理论支撑。 展开更多
关键词 蚕豆蛋白 11S蛋白 淀粉样纤维化聚集 结构特征 功能特性
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基于分布组件的应用服务器关键技术的研究——无状态会话Bean管理器的设计与实现 被引量:5
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作者 齐勇 侯迪 +1 位作者 赵季中 金健忠 《小型微型计算机系统》 CSCD 北大核心 2001年第7期855-859,共5页
中间层应用服务器是建立 Internet复杂环境下分布式应用系统的关键基础支撑平台 ;论文结合分布式对象组件技术 CORBA与 EJB,设计并实现了基于 Web环境的应用服务器中的关键技术之一——无状态会话 Bean管理器 .通过它可以完成应用服务... 中间层应用服务器是建立 Internet复杂环境下分布式应用系统的关键基础支撑平台 ;论文结合分布式对象组件技术 CORBA与 EJB,设计并实现了基于 Web环境的应用服务器中的关键技术之一——无状态会话 Bean管理器 .通过它可以完成应用服务器中相应的部署和管理功能 . 展开更多
关键词 应用服务器 事务处理 分布组件 无状态会话bean管理器 INTERNET
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Nutritional Value and Application of Broad Bean Nutrition Flour as a Combination of Staple Food 被引量:4
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作者 吴春芳 林晶晶 +2 位作者 卞晓春 陈惠 夏礼如 《Agricultural Science & Technology》 CAS 2015年第6期1280-1285,共6页
This paper introduced the definition and importance of combination of sta-ple food. With broad bean nutrition flour as a sample, the preparation of the combi-nation of staple food was also described. In addition, the ... This paper introduced the definition and importance of combination of sta-ple food. With broad bean nutrition flour as a sample, the preparation of the combi-nation of staple food was also described. In addition, the main nutritional value of broad bean nutrition flour was introduced. Compared with those of other single flours, the nutritional value of broad bean nutrition flour was improved. Moreover, the nutrients in the broad bean nutrition flour would not be destroyed during the processing and preparation of staple food, and the processed steamed bread and raw noodle are more characteristic. The application value and prospects of broad bean nutrition flour, as a combination of staple food, were further discussed. 展开更多
关键词 Broad bean nutrition flour Combination of staple food APPLICATION
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基于消息驱动Bean的异步通信模型设计 被引量:2
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作者 丁勇 杨鹤标 杜江 《计算机工程与设计》 CSCD 北大核心 2006年第10期1786-1788,1859,共4页
异步通信是一种松耦合的分布式通信模式,允许应用程序以发送方和接受方相互独立的形式,通过交换消息进行通信。消息驱动Bean作为处理异步消息的EJB组件,因其支持异步非阻塞的消息传递机制,在基于EJB的应用系统中日渐得到应用。提出了一... 异步通信是一种松耦合的分布式通信模式,允许应用程序以发送方和接受方相互独立的形式,通过交换消息进行通信。消息驱动Bean作为处理异步消息的EJB组件,因其支持异步非阻塞的消息传递机制,在基于EJB的应用系统中日渐得到应用。提出了一种基于消息驱动Bean的异步通信模型。该模型使用MDB实现消息的异步消费,解决了EJB远程方法调用开销大等问题,降低了应用程序和EJB组件之间的依赖性,从而有效地提高了异步通信的性能和可靠性。 展开更多
关键词 消息驱动 bean(MDB) Java消息服务(JMS) 企业版Java bean(EJB)
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Current Status and Development Strategy of Mung Bean Production in Chongqing Municipality 被引量:2
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作者 杜成章 张继君 +2 位作者 曾宪琪 黄世龙 张晓春 《Agricultural Science & Technology》 CAS 2012年第1期120-122,232,共4页
[Objective] This paper presented the current status of mung bean produc- tion in Chongqing, and put forward the corresponding development strategies. [Meth- ods] By overviewing the status of mung bean production in Ch... [Objective] This paper presented the current status of mung bean produc- tion in Chongqing, and put forward the corresponding development strategies. [Meth- ods] By overviewing the status of mung bean production in Chongqing Municipality, the main problems, advantages of mung bean production in Chongqing were pre- sented, and based on corresponding development strategies were put forward.[Re- suits] Currently, the main problems of mung bean production in Chongqing area in- clude insufficient attention, serious variety degradation, lag in cultivation technology, low scientific research level. However, the region has strengths in growing mung beans which would have broad prospects by appropriate adjustment and planning. [Conclusion] This study laid the foundation for the development of mung bean pro- duction and promotion of economic development in Chongqing Municipality. 展开更多
关键词 Chongqing Municipality Mung bean PRODUCTION DEVELOPMENT STRATEGIES
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Research Progress on Germplasm Resources of Common Bean 被引量:3
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作者 李斌 陈树良 +1 位作者 秦超 肖靖 《Agricultural Science & Technology》 CAS 2016年第11期2572-2577,共6页
The origin, classification, utilization and evaluation of germplasm resources, and breeding methods were introduced. The researches of biotic or/and abiotic stress, exploration of genomics and proteomics in common bea... The origin, classification, utilization and evaluation of germplasm resources, and breeding methods were introduced. The researches of biotic or/and abiotic stress, exploration of genomics and proteomics in common bean in recent years were reviewed in this paper, in order to provide theoretical reference for utilization and innovation of germplasm resources in common bean. 展开更多
关键词 Common bean Germplasm resources Biotic stress PROTEOMICS
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基于Java Bean组件技术的模型表示研究 被引量:6
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作者 孙禄 卢潇 张强 《电子设计工程》 2010年第10期9-12,共4页
为解决模型管理在模型重用、模型组合、跨平台调用等方面在目前网络应用环境下存在的问题,根据具体需求分析,指出现有表示方法的不足,在面向对象表示方法研究的基础上,结合组软件件技术的特点,比较了COM,CORBA,Bean 3种流行的组件技术后... 为解决模型管理在模型重用、模型组合、跨平台调用等方面在目前网络应用环境下存在的问题,根据具体需求分析,指出现有表示方法的不足,在面向对象表示方法研究的基础上,结合组软件件技术的特点,比较了COM,CORBA,Bean 3种流行的组件技术后,提出将Java Bean组建技术应用到模型表示方法中。通过规范模型的具体设计方法,对模型组合条件进行讨论,然后设计了模型的表示框架和基本组成部分。最后通过模型表示框架流程说明其可行性。 展开更多
关键词 模型管理 模型表示 组件技术 JAVA bean
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Genotypic Variation in Root Morphological Characteristics of Common Bean in Relation to Phosphorus Efficiency 被引量:10
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作者 廖红 严小龙 《Acta Botanica Sinica》 CSCD 2001年第11期1161-1166,共6页
Root morphology in plants may be related to phosphorus (P) efficiency by affecting the absorption characteristics of the root system. However, genotypic variation in root morphological characteristics of common bean (... Root morphology in plants may be related to phosphorus (P) efficiency by affecting the absorption characteristics of the root system. However, genotypic variation in root morphological characteristics of common bean ( Phaseolus vulgaris L.) as affected by P availability has not been well clarified. In the present study, systematic studies were conducted in a P-buffered sand culture system using three pairs of common bean parental materials with contrasting root traits in response to P deficiency. The results indicate that P availability significantly affects bean root morphology. Common bean tends to have smaller root system, shorter and coarser roots at low P availability. Genotypic variation in root morphology was observed among different genotypes in response to P availability. The P efficient genotypes appear to have larger, finer and longer root systems than the P inefficient genotypes, and such a variation was particularly obvious in the basal roots. From allomeric analysis, we found that morphological characteristics of the basal roots contribute more to P efficiency than those of the tap roots. Further studies with the F-9 recombinant inbred lines derived from one of the most contrasting parental pairs, DOR364 and G19833, confirmed the above findings, indicating that those morphological characteristics are inheritable hence provide potential for genetic improvement. 展开更多
关键词 common bean root morphology phosphorus efficiency genotypic variation
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Identification of Faba Bean(Vicia faba L.)Germplasm Resources in Hubei Province and Evaluation on Their Main Agronomic Traits 被引量:2
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作者 李莉 刘昌燕 +4 位作者 陈宏伟 万正煌 刘良军 伍广洪 黎大革 《Agricultural Science & Technology》 CAS 2017年第12期2219-2222,2228,共5页
Through field observation and lab analysis, 13 agronomic traits, including plant height and effective branching number per plant, etc., of 207 faba bean (Vicia faba L.) germplasms saved in Crop Middle-term Genebank ... Through field observation and lab analysis, 13 agronomic traits, including plant height and effective branching number per plant, etc., of 207 faba bean (Vicia faba L.) germplasms saved in Crop Middle-term Genebank of Hubei Province were evaluated, moreover, the identification on the disease resistance and the quality was carried out on parts of germplasm resources. The results showed that the faba beangermplasm resources in Hubei Province were abundant, the variation range of agronomic traits like the growth period, plant height and 100-bean weight, etc., was great, and the range of choice was wide. In the aspect of quality, there were 9 germplasms withthe content of crude protein higher than 30%, and the variation range of amylose content in total starch was large, which provided excellent sources of parents for breedinq hi.qh-quality faba bean varieties in Hubei Province. 展开更多
关键词 HUBEI Faba bean (Vicia faba L.) Variety resources Agronomic traits IDENTIFICATION
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Web应用服务器中实体BEAN的性能优化策略 被引量:1
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作者 杨波 张文博 +1 位作者 范国闯 陈宁江 《计算机科学》 CSCD 北大核心 2005年第1期120-124,共5页
Web应用服务器是Web计算环境下产生的新型中间件,为创建、部署、运行、集成和管理事务性Web应用提供一个跨平台运行环境。如何快速地响应大规模并发客户的请求,提供高可用性等特性是Web应用服务器需要解决的重点问题之一。本文采用ECper... Web应用服务器是Web计算环境下产生的新型中间件,为创建、部署、运行、集成和管理事务性Web应用提供一个跨平台运行环境。如何快速地响应大规模并发客户的请求,提供高可用性等特性是Web应用服务器需要解决的重点问题之一。本文采用ECperf作为性能测试基准,对影响实体Bean组件性能的瓶颈进行研究,给出若干优化策略,包括使用由容器管理实体Bean的持久化方式,选用更高级别的提交方式,声明只读的实体Bean等,并通过试验对优化的性能进行分析和比较。这些优化策略已应用到中科院软件所自主研制的WebFrame应用服务器中,取得了良好的效果。 展开更多
关键词 WEB应用服务器 实体bean 性能优化 J2EE ECperf
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FTIR Spectroscopic Study of Broad Bean Diseased Leaves 被引量:2
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作者 李志永 刘刚 +5 位作者 李伦 欧全宏 赵兴祥 张黎 周湘萍 汪禄祥 《Agricultural Science & Technology》 CAS 2012年第11期2363-2366,2408,共5页
[Objective] The aim was to indentify diseased leaves of broad bean by vibra- tional spectroscopy. [Method] In this paper, broad bean rust, fusarium rhizome rot, broad bean zonate spot, yellow leaf curl virus and norma... [Objective] The aim was to indentify diseased leaves of broad bean by vibra- tional spectroscopy. [Method] In this paper, broad bean rust, fusarium rhizome rot, broad bean zonate spot, yellow leaf curl virus and normal leaves were studied using Fourier transform infrared spectroscopy combined with chemometrics. [Result] The spectra of the samples were similar, only with minor differences in absorption inten- sity of several peaks. Second derivative analyses show that the significant difference of all samples was in the range of 1 200-700 cm2. The data in the range of 1 200- 700 cm' were selected to evaluate correlation coefficients, hierarchical cluster analy- sis (HCA) and principal component analysis (PCA). Results showed that the correla- tion coefficients are larger than 0.928 not only between the healthy leaves, but also between the same diseased leaves. The values between healthy and diseased leaves, and among diseased leaves, are all declined. HCA and PCA yielded about 73.3% and 82.2% accuracy, respectively. [Conclusion] This study demonstrated that FTIR techniques might be used to detect crop diseases. 展开更多
关键词 FTIR spectroscopy Broad bean diseases Principal component analysis Cluster analysis
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J2EE平台上消息驱动Bean对客户的响应与回传的实现 被引量:4
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作者 何文涛 林贤林 《计算机应用与软件》 CSCD 北大核心 2008年第5期181-182,184,共3页
EJB是基于Java的服务器端组件架构技术,借助于EJB,开发者能够轻松构建企业的、分布式组件应用,并且能够开发架构灵活、运行可靠及安全的应用系统。在EJB规范中,客户可向中间件发送消息,而消息驱动Bean(MDB)能从中间件接收消息,从而客户... EJB是基于Java的服务器端组件架构技术,借助于EJB,开发者能够轻松构建企业的、分布式组件应用,并且能够开发架构灵活、运行可靠及安全的应用系统。在EJB规范中,客户可向中间件发送消息,而消息驱动Bean(MDB)能从中间件接收消息,从而客户与消息驱动Bean实现异步传输消息,形成松耦合的状态。介绍了消息发布客户与消息驱动Bean通过服务器互传消息的理论,给出了Java消息服务的请求/响应解决方案,并且在J2EE1.4平台上实现了消息驱动Bean将响应结果异步回传给生产消息的客户,而客户可以是应用程序,Servlet/JSP,或EJB本身。 展开更多
关键词 J2EE1.4企业bean(EJB2.1) 消息驱动bean(MDB) Java消息服务(JMS)
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菌-酶协同法制备白芸豆抗氧化肽工艺优化及其抗氧化活性分析
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作者 谢晓妍 张芸溪 +8 位作者 王颖 刘童 李思楠 庞惟俏 佐兆杭 张璐 管骁 赵自力 李冰 《食品工业科技》 北大核心 2026年第3期207-220,共14页
为建立一种高效筛选白芸豆多肽(White kidney bean polypeptides,WKBPs)的方法并分析其体外抗氧化活性,本实验以白芸豆蛋白为原料,采用中性蛋白酶和枯草芽孢杆菌协同法制备WKBPs,通过单因素和响应面试验确定最佳工艺参数,并测定其氨基... 为建立一种高效筛选白芸豆多肽(White kidney bean polypeptides,WKBPs)的方法并分析其体外抗氧化活性,本实验以白芸豆蛋白为原料,采用中性蛋白酶和枯草芽孢杆菌协同法制备WKBPs,通过单因素和响应面试验确定最佳工艺参数,并测定其氨基酸组成和相对分子质量分布,利用超滤技术对制备的白芸豆粗肽进行分级分离,测定不同组分的抗氧化活性,对活性最好的组分进行液相色谱-质谱联用(LC-MS/MS)检测,并利用虚拟筛选、分子对接技术及分子动力学模拟探究肽段的潜在抗氧化活性。结果显示:菌-酶协同法制备WKBPs最佳工艺参数为发酵温度37.0℃、发酵时间48.0 h、接种量5.0%,此条件下的DPPH自由基清除率为76.12%±0.36%,多肽得率为17.59%±0.16%;WKBPs中疏水性氨基酸、碱性氨基酸和酸性氨基酸含量均较高,分别为21.932%、11.331%和20.583%;相对分子质量分布主要集中在3 kDa以下,超滤结果表明Mw<3 kDa的组分具有较好的抗氧化活性(DPPH自由基清除率、ABTS^(+)自由基清除率和羟自由基清除率),其半抑制浓度(IC_(50))值分别为0.307、0.310和0.361 mg/mL;总抗氧化能力(FRAP)在1 mg/mL的浓度下可以达到0.66±0.0018 mmol Fe^(2+)/g;通过虚拟筛选从LC-MS/MS鉴定的6364条肽序列中得到3条抗氧化多肽(FGWGP、FGHPEW和FGPYF),分子对接结果表明:肽段能够通过氢键及疏水相互作用等与Keap1结合形成稳定的复合物,调控Keap1-Nrf2通路发挥抗氧化作用;分子动力学模拟表明,FGWGP、FGHPEW和FGPYF可以与Keap1紧密结合形成稳定的构象。本实验为白芸豆蛋白的深度开发和白芸豆抗氧化肽产品的研发提供技术参考及理论基础。 展开更多
关键词 白芸豆多肽 菌-酶协同 超滤 抗氧化活性 分子对接
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