Introduction
According to the WHO's World Health Report 2003, 1cardiovascular disease was responsible for 16.7 million deaths annually. As a result, it is now the leading cause of death in developed count... Introduction
According to the WHO's World Health Report 2003, 1cardiovascular disease was responsible for 16.7 million deaths annually. As a result, it is now the leading cause of death in developed countries and in many developing countries. Detecting and preventing the progression of coronary artery disease is the target of many pharmaceutical, technological and other scientific programs today. Presently, the main diagnostic tool for evaluating coronary arteries is thc conventional coronary angiogram (CCA).……展开更多
Great Snipe(Gallinago media) is a shore bird which has a Near Threatened status on the global scale.However,little is known about its migration strategy from the breeding range in Russia.This study is the first one ai...Great Snipe(Gallinago media) is a shore bird which has a Near Threatened status on the global scale.However,little is known about its migration strategy from the breeding range in Russia.This study is the first one aiming to reveal migration routes,stopovers and wintering grounds of adult Great Snipes from their breeding range in Russia using GPS devices.We also analyzed connectivity of Great Snipes from different breeding populations of this species during non-breeding season.In 2021,we equipped seven males and three females with satellite transmitters,ICARUS Basic Tags,in the breeding range in central European Russia(56°75′N,37°65 E).One female appeared later in tundra of north-eastern Europe.In the second half of July to early September,birds migrated to Africa in a fairly wide front and made stopovers in Europe before crossing seas and the Sahara.Our data allowed to suppose high mortality of birds on migration,especially during the trans-Saharan flight.Only four Great Snipes reached Africa alive during southward migration.These birds spread over across wide area from Eritrea to Ghana after the trans-Saharan flight,after which they moved in a general westward direction and made final prolonged stopovers in Ghana or to the south of Chad Lake.In October/December birds relocated to wintering grounds in Sub-Equatorial Afrotropics as far as the south of Democratic Republic of the Congo and Zambia;with intermediate winter sites in low and middle reaches of the Congo Basin.Together with other published results,our data showed wide overlap of African non-breeding grounds of birds coming from lowland Eastern European and mountain Scandinavian breeding populations.The results also indicated insufficient conservation status of migration stopovers and wintering sites,used by Great Snipes,and demonstrated high importance of West Africa for conservation of this species.展开更多
Vaccination is a key strategy to curb the spread of epidemics.Heterologous vaccination,unlike homologous vaccination which acts on a single target and forms a single immune barrier,covers multiple targets for broader ...Vaccination is a key strategy to curb the spread of epidemics.Heterologous vaccination,unlike homologous vaccination which acts on a single target and forms a single immune barrier,covers multiple targets for broader protection.Yet,heterologous vaccination involves a complex decision process that conventional game-theoretic approaches,such as classical,evolutionary,and minority games cannot adequately capture.The parallel minority game(PMG)can handle bounded-rational,multi-choice decisions,but its application in vaccine research remains rare.In this study,we propose a vaccination-transmission coupled dynamic mechanism based on the parallel minority game and simulate it on a two-dimensional lattice.Using actual observational data and a mean-field mathematical model,we verify the effectiveness of this mechanism in simulating realistic vaccination behavior and transmission dynamics.We further analyze the impact of key parameters,such as vaccine efficacy differences and the proportion of individuals eligible for vaccine switching,on containment effectiveness.Our results demonstrate that heterologous vaccination surpasses homologous vaccination in containment effectiveness,particularly when vaccine efficacy varies significantly.This work provides a novel framework and empirical evidence for understanding individual decision-making and population-wide immunity formation in multi-vaccine settings.展开更多
Agricultural development is regarded as the most important foundation of China’s modernisation.According to the Recommendations of the CPC Central Committee for Formulating the 15th Five-Year Plan for National Econom...Agricultural development is regarded as the most important foundation of China’s modernisation.According to the Recommendations of the CPC Central Committee for Formulating the 15th Five-Year Plan for National Economic and Social Development(2026–2030),issues related to agriculture,rural areas and rural residents should be given top priority.In the coming years,China will promote coordinated urban and rural development,continue to consolidate and expand the achievements of poverty alleviation,ensure basic modern living conditions in rural areas,and accelerate progress in building a strong agricultural sector.展开更多
AAV-PHP.eB is an artificial adeno-associated virus(AAV)that crosses the blood-brain barrier and targets neurons more efficiently than other AAVs when administered systematically.While AAV-PHP.eB has been used in vario...AAV-PHP.eB is an artificial adeno-associated virus(AAV)that crosses the blood-brain barrier and targets neurons more efficiently than other AAVs when administered systematically.While AAV-PHP.eB has been used in various disease models,its cellular tropism in cerebrovascular diseases remains unclear.In the present study,we aimed to elucidate the tropism of AAV-PHP.eB for different cell types in the brain in a mouse model of ischemic stroke and evaluate its effectiveness in mediating basic fibroblast growth factor(bFGF)gene therapy.Mice were injected intravenously with AAV-PHP.eB either 14 days prior to(pre-stroke)or 1 day following(post-stroke)transient middle cerebral artery occlusion.Notably,we observed a shift in tropism from neurons to endothelial cells with post-stroke administration of AAV-PHP.eB-mNeonGreen(mNG).This endothelial cell tropism correlated strongly with expression of the endothelial membrane receptor lymphocyte antigen 6 family member A(Ly6A).Furthermore,AAV-PHP.eB-mediated overexpression of bFGF markedly improved neurobehavioral outcomes and promoted long-term neurogenesis and angiogenesis post-ischemic stroke.Our findings underscore the significance of considering potential tropism shifts when utilizing AAV-PHP.eB-mediated gene therapy in neurological diseases and suggest a promising new strategy for bFGF gene therapy in stroke treatment.展开更多
Objectives:The current treatment options and therapeutic targets for triple-negative breast cancer(TNBC),an aggressive subtype of breast cancer(BrCA),are limited.This study aimed to identify novel biomarkers and trans...Objectives:The current treatment options and therapeutic targets for triple-negative breast cancer(TNBC),an aggressive subtype of breast cancer(BrCA),are limited.This study aimed to identify novel biomarkers and transcriptional regulatory networks(TRN)inherent in TNBC samples.Methods:We analyzed pan-cancer BrCA datasets from The Cancer Genome Atlas(TCGA)to compare triple-positive breast cancer(TPBC)with TNBC.TRN algorithms and virtual inference of protein-enriched regulon(VIPER)were used to identify master regulators and their target genes.Utilizing TNBC cells(MDA-MB-231 and MDA-MB-468),we validated the relationship of nuclear factor erythroid 2-like 3(NFE2L3)and basic helix-loop-helix family member E 40(BHLHE40)by performing a luciferase assay.The expression levels of these targets were measured after transfections with plasmid and siRNA via qRT-PCR and western blots.The effect of these genes on cell proliferation and migration was studied using phenotypic assays.Results:Using computational approaches,we identified NFE2L3 as a master regulator with BHLHE40 as its target gene.NFE2L3 protein binds to the promoter region of BHLHE40 and regulates its transcriptional activity.Additionally,silencing and overexpressing NFE2L3 and BHLHE40 in TNBC cell lines MDA-MB-231 and MDA-MB-468 showed that NFE2L3 directly regulates BHLHE40 at both transcriptional and translational levels.We found that BHLHE40 requires NFE2L3 for cell proliferation and migration in TNBC.Conclusion:These findings underscore the significance of NFE2L3 and BHLHE40 in TNBC,highlighting NFE2L3’s role in regulating the oncogenic activity of BHLHE40 in TNBC cells.展开更多
There is so much terminology floating around with this issue that it is hard to make any sense of what it’s all about. That’s why you have to start by understanding what they’re talking about: the "good,"...There is so much terminology floating around with this issue that it is hard to make any sense of what it’s all about. That’s why you have to start by understanding what they’re talking about: the "good," the "bad, " and the ugly. 本文术语遍布,内容艰涩。因此你必须首先了解他们谈的内容:“好的”、“坏的”、还有丑陋的。展开更多
The purpose of this article is to provide an accessible introduction to foundational statistical procedures and present the steps of data analysis to address research questions and meet standards for scientific rigour...The purpose of this article is to provide an accessible introduction to foundational statistical procedures and present the steps of data analysis to address research questions and meet standards for scientific rigour.It is aimed at individuals new to research with less familiarity with statistics,or anyone interested in reviewing basic statistics.After examining a brief overview of foundational statistical techniques,for example,differences between descriptive and inferential statistics,the article illustrates 10 steps in conducting statistical analysis with examples of each.The following are the general steps for statistical analysis:(1)formulate a hypothesis,(2)select an appropriate statistical test,(3)conduct a power analysis,(4)prepare data for analysis,(5)start with descriptive statistics,(6)check assumptions of tests,(7)run the analysis,(8)examine the statistical model,(9)report the results and(10)evaluate threats to validity of the statistical analysis.Researchers in family medicine and community health can follow specific steps to ensure a systematic and rigorous analysis.展开更多
The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after injury.However,whether it is possible to stimulate ne...The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after injury.However,whether it is possible to stimulate neurogenesis and reconstruct cortical layers II to VI in non-neurogenic regions,such as the cortex,remains unknown.In this study,we implanted a hyaluronic acid collagen gel loaded with basic fibroblast growth factor into the motor cortex immediately following traumatic injury.Our findings reveal that this gel effectively stimulated the proliferation and migration of endogenous neural stem/progenitor cells,as well as their differentiation into mature and functionally integrated neurons.Importantly,these new neurons reconstructed the architecture of cortical layers II to VI,integrated into the existing neural circuitry,and ultimately led to improved brain function.These findings offer novel insight into potential clinical treatments for traumatic cerebral cortex injuries.展开更多
Cardiovascular diseases(CVD)and their risk factors are exerting an increasingly significant impact on public health,and the incidence rate of CVD continues to rise.This article provides an interpretation of essentials...Cardiovascular diseases(CVD)and their risk factors are exerting an increasingly significant impact on public health,and the incidence rate of CVD continues to rise.This article provides an interpretation of essentials from the newly published Annual Report on Cardiovascular Health and Diseases in China(2024),aiming to offer scientific evidence for CVD prevention,treatment,and the formulation of relevant policies.展开更多
The selection of an appropriate basic detonation wave flow field is crucial for improving the performance and geometric design of standing detonation vehicles.This paper employs a detailed chemical reaction model and ...The selection of an appropriate basic detonation wave flow field is crucial for improving the performance and geometric design of standing detonation vehicles.This paper employs a detailed chemical reaction model and solves the unsteady axisymmetric Euler equation to study the characteristics of the Axisymmetric Inward Turning Curved Detonation Wave(AIT-CDW)flow field and the parameters affecting the stability of the wave system structure of AIT-CDW flow field.The numerical results demonstrate a radial compression effect in the AIT-CDW flow field.This effect causes the detonation wave to have a shorter initiation length than oblique detonation wave flow field and the detonation wave angle to gradually increase with the flow direction postdetonation.The AIT-CDW flow field is confined space,making it prone to normal detonation waves when the detonation wave reflects from the wall.This phenomenon is detrimental to the stability of the wave system structure in the flow field.It has been observed that increasing the center body radius and decreasing the fuel equivalent ratio can effectively reduce the height of the normal detonation wave or even eliminate it.Additionally,a well-designed generatrix shape of the center body can enhance airflow,reduce choked flow,and promote the stability of the wave structure in the flow field.展开更多
文摘 Introduction
According to the WHO's World Health Report 2003, 1cardiovascular disease was responsible for 16.7 million deaths annually. As a result, it is now the leading cause of death in developed countries and in many developing countries. Detecting and preventing the progression of coronary artery disease is the target of many pharmaceutical, technological and other scientific programs today. Presently, the main diagnostic tool for evaluating coronary arteries is thc conventional coronary angiogram (CCA).……
基金A support for different activities in the framework of this research was provided by Max Planck Institute of Animal Behavior,the Russian space agency (Roskosmos),German Aerospace Center and Institute for Geography of the Russian Academy of Sciences via International Cooperation for Animal Research Using Space as well as by NABU–The Nature and Biodiversity Conservation Union2021 was carried out as a joint project of Birds Russia and Manfred-Hermsen Stiftung+2 种基金funded under state assignments of the Severtsov Institute of Ecology and Evolution of the Russian Academy of Science (No. 0089-2021-0004, FFER-2024-0013No. 0089-2021-0010, FFER-2024-0022No. 1022040700480-0-1.6.15)。
文摘Great Snipe(Gallinago media) is a shore bird which has a Near Threatened status on the global scale.However,little is known about its migration strategy from the breeding range in Russia.This study is the first one aiming to reveal migration routes,stopovers and wintering grounds of adult Great Snipes from their breeding range in Russia using GPS devices.We also analyzed connectivity of Great Snipes from different breeding populations of this species during non-breeding season.In 2021,we equipped seven males and three females with satellite transmitters,ICARUS Basic Tags,in the breeding range in central European Russia(56°75′N,37°65 E).One female appeared later in tundra of north-eastern Europe.In the second half of July to early September,birds migrated to Africa in a fairly wide front and made stopovers in Europe before crossing seas and the Sahara.Our data allowed to suppose high mortality of birds on migration,especially during the trans-Saharan flight.Only four Great Snipes reached Africa alive during southward migration.These birds spread over across wide area from Eritrea to Ghana after the trans-Saharan flight,after which they moved in a general westward direction and made final prolonged stopovers in Ghana or to the south of Chad Lake.In October/December birds relocated to wintering grounds in Sub-Equatorial Afrotropics as far as the south of Democratic Republic of the Congo and Zambia;with intermediate winter sites in low and middle reaches of the Congo Basin.Together with other published results,our data showed wide overlap of African non-breeding grounds of birds coming from lowland Eastern European and mountain Scandinavian breeding populations.The results also indicated insufficient conservation status of migration stopovers and wintering sites,used by Great Snipes,and demonstrated high importance of West Africa for conservation of this species.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.12571549,12571592,12471463,12022113,12101573)。
文摘Vaccination is a key strategy to curb the spread of epidemics.Heterologous vaccination,unlike homologous vaccination which acts on a single target and forms a single immune barrier,covers multiple targets for broader protection.Yet,heterologous vaccination involves a complex decision process that conventional game-theoretic approaches,such as classical,evolutionary,and minority games cannot adequately capture.The parallel minority game(PMG)can handle bounded-rational,multi-choice decisions,but its application in vaccine research remains rare.In this study,we propose a vaccination-transmission coupled dynamic mechanism based on the parallel minority game and simulate it on a two-dimensional lattice.Using actual observational data and a mean-field mathematical model,we verify the effectiveness of this mechanism in simulating realistic vaccination behavior and transmission dynamics.We further analyze the impact of key parameters,such as vaccine efficacy differences and the proportion of individuals eligible for vaccine switching,on containment effectiveness.Our results demonstrate that heterologous vaccination surpasses homologous vaccination in containment effectiveness,particularly when vaccine efficacy varies significantly.This work provides a novel framework and empirical evidence for understanding individual decision-making and population-wide immunity formation in multi-vaccine settings.
文摘Agricultural development is regarded as the most important foundation of China’s modernisation.According to the Recommendations of the CPC Central Committee for Formulating the 15th Five-Year Plan for National Economic and Social Development(2026–2030),issues related to agriculture,rural areas and rural residents should be given top priority.In the coming years,China will promote coordinated urban and rural development,continue to consolidate and expand the achievements of poverty alleviation,ensure basic modern living conditions in rural areas,and accelerate progress in building a strong agricultural sector.
基金supported by the National Natural Science Foundation of China,Nos.81870921(to YW),81974179(to ZZ),82271320(to ZZ),82071284(to YT)National Key R&D Program of China,No.2022YFA1603600(to ZZ),2019YFA0112000(to YT)+1 种基金Scientific Research and Innovation Program of Shanghai Education Commission,No.2019-01-07-00-02-E00064(to GYY)Scientific and Technological Innovation Act Program of Shanghai Science and Technology Commission,No.20JC1411900(to GYY).
文摘AAV-PHP.eB is an artificial adeno-associated virus(AAV)that crosses the blood-brain barrier and targets neurons more efficiently than other AAVs when administered systematically.While AAV-PHP.eB has been used in various disease models,its cellular tropism in cerebrovascular diseases remains unclear.In the present study,we aimed to elucidate the tropism of AAV-PHP.eB for different cell types in the brain in a mouse model of ischemic stroke and evaluate its effectiveness in mediating basic fibroblast growth factor(bFGF)gene therapy.Mice were injected intravenously with AAV-PHP.eB either 14 days prior to(pre-stroke)or 1 day following(post-stroke)transient middle cerebral artery occlusion.Notably,we observed a shift in tropism from neurons to endothelial cells with post-stroke administration of AAV-PHP.eB-mNeonGreen(mNG).This endothelial cell tropism correlated strongly with expression of the endothelial membrane receptor lymphocyte antigen 6 family member A(Ly6A).Furthermore,AAV-PHP.eB-mediated overexpression of bFGF markedly improved neurobehavioral outcomes and promoted long-term neurogenesis and angiogenesis post-ischemic stroke.Our findings underscore the significance of considering potential tropism shifts when utilizing AAV-PHP.eB-mediated gene therapy in neurological diseases and suggest a promising new strategy for bFGF gene therapy in stroke treatment.
文摘Objectives:The current treatment options and therapeutic targets for triple-negative breast cancer(TNBC),an aggressive subtype of breast cancer(BrCA),are limited.This study aimed to identify novel biomarkers and transcriptional regulatory networks(TRN)inherent in TNBC samples.Methods:We analyzed pan-cancer BrCA datasets from The Cancer Genome Atlas(TCGA)to compare triple-positive breast cancer(TPBC)with TNBC.TRN algorithms and virtual inference of protein-enriched regulon(VIPER)were used to identify master regulators and their target genes.Utilizing TNBC cells(MDA-MB-231 and MDA-MB-468),we validated the relationship of nuclear factor erythroid 2-like 3(NFE2L3)and basic helix-loop-helix family member E 40(BHLHE40)by performing a luciferase assay.The expression levels of these targets were measured after transfections with plasmid and siRNA via qRT-PCR and western blots.The effect of these genes on cell proliferation and migration was studied using phenotypic assays.Results:Using computational approaches,we identified NFE2L3 as a master regulator with BHLHE40 as its target gene.NFE2L3 protein binds to the promoter region of BHLHE40 and regulates its transcriptional activity.Additionally,silencing and overexpressing NFE2L3 and BHLHE40 in TNBC cell lines MDA-MB-231 and MDA-MB-468 showed that NFE2L3 directly regulates BHLHE40 at both transcriptional and translational levels.We found that BHLHE40 requires NFE2L3 for cell proliferation and migration in TNBC.Conclusion:These findings underscore the significance of NFE2L3 and BHLHE40 in TNBC,highlighting NFE2L3’s role in regulating the oncogenic activity of BHLHE40 in TNBC cells.
文摘There is so much terminology floating around with this issue that it is hard to make any sense of what it’s all about. That’s why you have to start by understanding what they’re talking about: the "good," the "bad, " and the ugly. 本文术语遍布,内容艰涩。因此你必须首先了解他们谈的内容:“好的”、“坏的”、还有丑陋的。
基金funded by the National Institutes of Health(10.13039/100000002)and grant number 1K01LM012739.
文摘The purpose of this article is to provide an accessible introduction to foundational statistical procedures and present the steps of data analysis to address research questions and meet standards for scientific rigour.It is aimed at individuals new to research with less familiarity with statistics,or anyone interested in reviewing basic statistics.After examining a brief overview of foundational statistical techniques,for example,differences between descriptive and inferential statistics,the article illustrates 10 steps in conducting statistical analysis with examples of each.The following are the general steps for statistical analysis:(1)formulate a hypothesis,(2)select an appropriate statistical test,(3)conduct a power analysis,(4)prepare data for analysis,(5)start with descriptive statistics,(6)check assumptions of tests,(7)run the analysis,(8)examine the statistical model,(9)report the results and(10)evaluate threats to validity of the statistical analysis.Researchers in family medicine and community health can follow specific steps to ensure a systematic and rigorous analysis.
基金supported by the National Natural Science Foundation of China,Nos.82272171(to ZY),82271403(to XL),81941011(to XL),31971279(to ZY),31730030(to XL)the Natural Science Foundation of Beijing,No.7222004(to HD).
文摘The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after injury.However,whether it is possible to stimulate neurogenesis and reconstruct cortical layers II to VI in non-neurogenic regions,such as the cortex,remains unknown.In this study,we implanted a hyaluronic acid collagen gel loaded with basic fibroblast growth factor into the motor cortex immediately following traumatic injury.Our findings reveal that this gel effectively stimulated the proliferation and migration of endogenous neural stem/progenitor cells,as well as their differentiation into mature and functionally integrated neurons.Importantly,these new neurons reconstructed the architecture of cortical layers II to VI,integrated into the existing neural circuitry,and ultimately led to improved brain function.These findings offer novel insight into potential clinical treatments for traumatic cerebral cortex injuries.
文摘Cardiovascular diseases(CVD)and their risk factors are exerting an increasingly significant impact on public health,and the incidence rate of CVD continues to rise.This article provides an interpretation of essentials from the newly published Annual Report on Cardiovascular Health and Diseases in China(2024),aiming to offer scientific evidence for CVD prevention,treatment,and the formulation of relevant policies.
基金supported by the National Natural Science Foundation of China(Nos.U20A2069,62376234 and 123B2037)the Advanced Aero-Power Innovation Workstation,China(No.HKCX2024-01-017)。
文摘The selection of an appropriate basic detonation wave flow field is crucial for improving the performance and geometric design of standing detonation vehicles.This paper employs a detailed chemical reaction model and solves the unsteady axisymmetric Euler equation to study the characteristics of the Axisymmetric Inward Turning Curved Detonation Wave(AIT-CDW)flow field and the parameters affecting the stability of the wave system structure of AIT-CDW flow field.The numerical results demonstrate a radial compression effect in the AIT-CDW flow field.This effect causes the detonation wave to have a shorter initiation length than oblique detonation wave flow field and the detonation wave angle to gradually increase with the flow direction postdetonation.The AIT-CDW flow field is confined space,making it prone to normal detonation waves when the detonation wave reflects from the wall.This phenomenon is detrimental to the stability of the wave system structure in the flow field.It has been observed that increasing the center body radius and decreasing the fuel equivalent ratio can effectively reduce the height of the normal detonation wave or even eliminate it.Additionally,a well-designed generatrix shape of the center body can enhance airflow,reduce choked flow,and promote the stability of the wave structure in the flow field.
