A set of basic deformation modes for hybrid stress finite elements are directly derived from the element displacement field. Subsequently, by employing the so-called united orthogonal conditions, a new orthogonalizati...A set of basic deformation modes for hybrid stress finite elements are directly derived from the element displacement field. Subsequently, by employing the so-called united orthogonal conditions, a new orthogonalization method is proposed. The result- ing orthogonal basic deformation modes exhibit simple and clear physical meanings. In addition, they do not involve any material parameters, and thus can be efficiently used to examine the element performance and serve as a unified tool to assess different hybrid elements. Thereafter, a convenient approach for the identification of spurious zero-energy modes is presented using the positive definiteness property of a flexibility matrix. More- over, based on the orthogonality relationship between the given initial stress modes and the orthogonal basic deformation modes, an alternative method of assumed stress modes to formulate a hybrid element free of spurious modes is discussed. It is found that the orthogonality of the basic deformation modes is the sufficient and necessary condition for the suppression of spurious zero-energy modes. Numerical examples of 2D 4-node quadrilateral elements and 3D 8-node hexahedral elements are illustrated in detail to demonstrate the efficiency of the proposed orthogonal basic deformation mode method.展开更多
In this paper a method is developed to model the design process gene based on the extensible basic-element with the purpose of design process optimization and reuse. First, the principle of genetic engineering based d...In this paper a method is developed to model the design process gene based on the extensible basic-element with the purpose of design process optimization and reuse. First, the principle of genetic engineering based design process optimization and reuse is put forward and analyzed. Second, the extensible basic-element model of the design process gene is established based on the models of the design process base and the base pair through analyzing the concept and structure of the design process gene and the extensible basic-element as well as its extensibility. Third, the features of divergence and scalability of the extensible basic-element model of the design process gene are discussed for carrying out the extension translation to the design process gene by way of inserting, deleting and updating design process bases. Finally, an example of building extensible basic-element models for the design process base, base pair and design process gene in mechanical product design and the mutation process of the design process gene in airplane design is presented which demonstrates the application of the method proposed in this paper.展开更多
BACKGROUND： cAMP-response element binding protein （CREB） is a key modulator of various signaling pathways. CREB activation initiates a series of intracellular signaling pathways that promote neuronal survival. OBJE...BACKGROUND： cAMP-response element binding protein （CREB） is a key modulator of various signaling pathways. CREB activation initiates a series of intracellular signaling pathways that promote neuronal survival. OBJECTIVE： To investigate the regulatory effects of basic fibroblast growth factor （bFGF） on cerebral neuronal CREB expression following ischemia/reperfusion injury. DESIGN, TIME AND SETTING： An immunohistochemical detection experiment was performed at the Department of Anatomy, Shenyang Medical College, between October 2006 and April 2008. MATERIALS： A total of 60 healthy, adult, Wistar rats were randomly divided into three groups： sham-operated （n =12）, ischemia/reperfusion （n = 24）, and bFGF-treated （n = 24）. Rabbit anti-rat CREB （1： 100） and biotin labeled goat anti-rabbit IgG were purchased from the Wuhan Boster Company, China. MetaMorph-evolution MP5.0-BX51 microscopy imaging system was provided by China Medical University, China. METHODS： Rat models of cerebral ischemia/reperfusion injury were developed using the suture method for right middle cerebral artery occlusion. Two-hour ischemia was followed by reperfusion. Rats from the bFGF-treated and ischemia/reperfusion groups were intraperitoneally administered endogenous bFGF （500 IU/mL, 2 000 IU/kg） or an equal amount of physiological saline. Rats from the sham-operated group underwent a similar surgical procedure, without induction of ischemia/reperfusion injury and drug administration. MAIN OUTCOME MEASURES： After 48-hour reperfusion, hippocampal and parietal cortical neuronal CREB expression was detected by immunohistochemistry, and the absorbance of hippocampal CREB-positive products was determined using MetaMorph-evolutionMP5.0-BX51 microscopy imaging system. RESULTS： The sham-operated group exhibited noticeable CREB expression in hippocampal and parietal cortical neurons. In the ischemia/reperfusion group, the CREB expression was discrete and neurons were poorly arranged. The bFGF-treated group exhibited increased CREB expression and better neuronal arrangement compared with the ischemia/reperfusion group. The mean absorbance of CREB-immunoreactive products in the hippocampus and parietal cortex was significantly higher in the ischemia/reperfusion group than in the sham-operated group （P 〈 0.05）, and significantly higher in the bFGF-treated group than in the ischemia/reperfusion group （P 〈 0.05）. CONCLUSION： bFGF significantly upregulates CREB expression in hippocampal and parietal cortical neurons following ischemia/reperfusion injury.展开更多
基金Project supported by the National Natural Science Foundation of China(No.10972188)the Fundamental Research Funds for the Central Universities of China(No.2010121073)the Scientific Program of Fujian Province of China(No.2007F3096)
文摘A set of basic deformation modes for hybrid stress finite elements are directly derived from the element displacement field. Subsequently, by employing the so-called united orthogonal conditions, a new orthogonalization method is proposed. The result- ing orthogonal basic deformation modes exhibit simple and clear physical meanings. In addition, they do not involve any material parameters, and thus can be efficiently used to examine the element performance and serve as a unified tool to assess different hybrid elements. Thereafter, a convenient approach for the identification of spurious zero-energy modes is presented using the positive definiteness property of a flexibility matrix. More- over, based on the orthogonality relationship between the given initial stress modes and the orthogonal basic deformation modes, an alternative method of assumed stress modes to formulate a hybrid element free of spurious modes is discussed. It is found that the orthogonality of the basic deformation modes is the sufficient and necessary condition for the suppression of spurious zero-energy modes. Numerical examples of 2D 4-node quadrilateral elements and 3D 8-node hexahedral elements are illustrated in detail to demonstrate the efficiency of the proposed orthogonal basic deformation mode method.
文摘In this paper a method is developed to model the design process gene based on the extensible basic-element with the purpose of design process optimization and reuse. First, the principle of genetic engineering based design process optimization and reuse is put forward and analyzed. Second, the extensible basic-element model of the design process gene is established based on the models of the design process base and the base pair through analyzing the concept and structure of the design process gene and the extensible basic-element as well as its extensibility. Third, the features of divergence and scalability of the extensible basic-element model of the design process gene are discussed for carrying out the extension translation to the design process gene by way of inserting, deleting and updating design process bases. Finally, an example of building extensible basic-element models for the design process base, base pair and design process gene in mechanical product design and the mutation process of the design process gene in airplane design is presented which demonstrates the application of the method proposed in this paper.
基金Scientific Research Foundation of Liaoning Provincial Education Department for Higher Education Institutions, No.05L442
文摘BACKGROUND： cAMP-response element binding protein （CREB） is a key modulator of various signaling pathways. CREB activation initiates a series of intracellular signaling pathways that promote neuronal survival. OBJECTIVE： To investigate the regulatory effects of basic fibroblast growth factor （bFGF） on cerebral neuronal CREB expression following ischemia/reperfusion injury. DESIGN, TIME AND SETTING： An immunohistochemical detection experiment was performed at the Department of Anatomy, Shenyang Medical College, between October 2006 and April 2008. MATERIALS： A total of 60 healthy, adult, Wistar rats were randomly divided into three groups： sham-operated （n =12）, ischemia/reperfusion （n = 24）, and bFGF-treated （n = 24）. Rabbit anti-rat CREB （1： 100） and biotin labeled goat anti-rabbit IgG were purchased from the Wuhan Boster Company, China. MetaMorph-evolution MP5.0-BX51 microscopy imaging system was provided by China Medical University, China. METHODS： Rat models of cerebral ischemia/reperfusion injury were developed using the suture method for right middle cerebral artery occlusion. Two-hour ischemia was followed by reperfusion. Rats from the bFGF-treated and ischemia/reperfusion groups were intraperitoneally administered endogenous bFGF （500 IU/mL, 2 000 IU/kg） or an equal amount of physiological saline. Rats from the sham-operated group underwent a similar surgical procedure, without induction of ischemia/reperfusion injury and drug administration. MAIN OUTCOME MEASURES： After 48-hour reperfusion, hippocampal and parietal cortical neuronal CREB expression was detected by immunohistochemistry, and the absorbance of hippocampal CREB-positive products was determined using MetaMorph-evolutionMP5.0-BX51 microscopy imaging system. RESULTS： The sham-operated group exhibited noticeable CREB expression in hippocampal and parietal cortical neurons. In the ischemia/reperfusion group, the CREB expression was discrete and neurons were poorly arranged. The bFGF-treated group exhibited increased CREB expression and better neuronal arrangement compared with the ischemia/reperfusion group. The mean absorbance of CREB-immunoreactive products in the hippocampus and parietal cortex was significantly higher in the ischemia/reperfusion group than in the sham-operated group （P 〈 0.05）, and significantly higher in the bFGF-treated group than in the ischemia/reperfusion group （P 〈 0.05）. CONCLUSION： bFGF significantly upregulates CREB expression in hippocampal and parietal cortical neurons following ischemia/reperfusion injury.
