Abstract： One of the great concerns of the customers is the traceability of the products. The authors proposed to link microbial ecology to geographical origin of foodstuffs by a molecular technique joined to an imag...Abstract： One of the great concerns of the customers is the traceability of the products. The authors proposed to link microbial ecology to geographical origin of foodstuffs by a molecular technique joined to an image analysis. Molecular techniques employing 16S and 28S rDNA profiles generated by PCR-DGGE were used to detect the variation in microbial community （bacteria, fungi） of Pangasius fish from Viet Nam harvested in different aquaculture farms and during different seasons and Shea tree fruits from five different districts in Mali. The bacterial DNA profiles from Pangasius fish and the fungal DNA profile from Shea tree fruits were specific to each place of production and could be used as a biological bar code certifying the origin of fish and fruit. To follow the product during processing, the authors proposed to identify and validate some pertinent biological markers which come from the environment of the food to assure their traceability during international trade. It is new analytical method which permits to determine the origin of food or to follow them during international trade.展开更多
Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is importan...Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is important to identify readers from cell samples so as to understand biological roles of lysine methylation.Generally,readers bind methyllysine via aromatic cages that contain tryptophan,tyrosine and phenylalanine,that offer a unique motif for selective crosslinking.We recently reported a site-selective tryptophan crosslinking strategy based on dimethylsulfonium that mimics dimethyllysine to crosslink tryptophan in aromatic cages of readers.Since tyrosine is a key residue for binding affinity to methyllysine,especially some readers that do not contain tryptophan residues in the binding pocket.Here we developed strategies of site-selective crosslinking to tyrosine.Ultraviolet(UV)source was applied to excite tyrosine at neutral pH or phenoxide at basic p H,and subsequent single-electron transfer(SET)from Tyr*to sulfonium inside the binding pocket enables selective crosslinking.In consequence,methyllysine readers with tyrosine-containing aromatic cages could be selectively crosslinked by site-specific sulfonium peptide probes.In addition,we expanded substrates from aromatic cages to tyrosine residues of proximate contact with sulfonium probes.The pair of LgBiT and SmBiT exhibited orthogonal crosslinking in complicated cell samples.As a result,we may expand sulfonium tools to target local tyrosine in future investigations.展开更多
In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education ...In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education and reading experience,face challenges such as unclear leveling logic,insufficient functional support,and weak user engagement.This paper introduces the 4V marketing theory and constructs an analytical framework from four dimensions:differentiation,functionality,added value,and resonance.Two representative products,“Climbing Mathematics”and“Spark Mathematics,”are selected for a typical case comparison to identify their strengths and weaknesses in content design,service systems,and brand operation,and to extract transferable strategic elements.The study finds that the user-value-oriented strategy based on the 4V model can effectively address the core issues in the market promotion and user relationship building of leveled mathematics readers,providing practical paths and theoretical support for educational publishing institutions to achieve product innovation and brand upgrading in this niche field.展开更多
A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional G...A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional Gilbert mixer pair in commutating mode. A quadrature down-conversion mixer based on this topology is designed and optimized for an ultra high frequency RFID reader. Operating in the 915MHz ISM band, the presented quadrature mixer measures a conversion gain of 12.5dB,an IIP3 of 10dBm, an IIP2 of 58dBm, and an SSB noise figure of 17.6riB. The chip was fabricated in a 0. 18μm 1P6M RF CMOS process and consumes only 3mA of current from a 1.8V power supply.展开更多
A direct conversion receiver with optimized tolerance to local carrier interference is designed and implemented in a 0.18μm 1P6M mixed-signal CMOS process for a 900MHz RFID reader transceiver. A baseband amplifier wi...A direct conversion receiver with optimized tolerance to local carrier interference is designed and implemented in a 0.18μm 1P6M mixed-signal CMOS process for a 900MHz RFID reader transceiver. A baseband amplifier with series feedback topology is proposed to achieve passive mixer buffering,baseband DC cancellation,and signal amplification simultaneously. The receiver has a measured input ldB compression point of - 4dBm and a sensitivity of - 70dBm when 10dB SNR for digital demodulation is required. The receiver is integrated in a reader transceiver chip and consumes 90mA from a 1.8V supply.展开更多
Henry James' s first novella Daisy Miller brought him popularity and literary reputation after its publication. Through the application of reader theory and a close reading of the text, this article analyzes Daisy...Henry James' s first novella Daisy Miller brought him popularity and literary reputation after its publication. Through the application of reader theory and a close reading of the text, this article analyzes Daisy's strong personality and her misfortunes during her trip to Europe, and reveals how the novella highlights the conflict between American frankness and innocence and European sophistication. Besides, this article also elaborates on James' unique artistic skills—central consciousness and psychological analysis through his choice of narrative point of view, which contribute to the theme and the artistic charm of DaisyMiller.展开更多
A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration...A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U.展开更多
文摘Abstract： One of the great concerns of the customers is the traceability of the products. The authors proposed to link microbial ecology to geographical origin of foodstuffs by a molecular technique joined to an image analysis. Molecular techniques employing 16S and 28S rDNA profiles generated by PCR-DGGE were used to detect the variation in microbial community （bacteria, fungi） of Pangasius fish from Viet Nam harvested in different aquaculture farms and during different seasons and Shea tree fruits from five different districts in Mali. The bacterial DNA profiles from Pangasius fish and the fungal DNA profile from Shea tree fruits were specific to each place of production and could be used as a biological bar code certifying the origin of fish and fruit. To follow the product during processing, the authors proposed to identify and validate some pertinent biological markers which come from the environment of the food to assure their traceability during international trade. It is new analytical method which permits to determine the origin of food or to follow them during international trade.
基金the support from National Natural Science Foundation of China(No.22161132006)Key R&D Program of Zhejiang(No.2024SSYS0036)Westlake University Startup。
文摘Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is important to identify readers from cell samples so as to understand biological roles of lysine methylation.Generally,readers bind methyllysine via aromatic cages that contain tryptophan,tyrosine and phenylalanine,that offer a unique motif for selective crosslinking.We recently reported a site-selective tryptophan crosslinking strategy based on dimethylsulfonium that mimics dimethyllysine to crosslink tryptophan in aromatic cages of readers.Since tyrosine is a key residue for binding affinity to methyllysine,especially some readers that do not contain tryptophan residues in the binding pocket.Here we developed strategies of site-selective crosslinking to tyrosine.Ultraviolet(UV)source was applied to excite tyrosine at neutral pH or phenoxide at basic p H,and subsequent single-electron transfer(SET)from Tyr*to sulfonium inside the binding pocket enables selective crosslinking.In consequence,methyllysine readers with tyrosine-containing aromatic cages could be selectively crosslinked by site-specific sulfonium peptide probes.In addition,we expanded substrates from aromatic cages to tyrosine residues of proximate contact with sulfonium probes.The pair of LgBiT and SmBiT exhibited orthogonal crosslinking in complicated cell samples.As a result,we may expand sulfonium tools to target local tyrosine in future investigations.
文摘In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education and reading experience,face challenges such as unclear leveling logic,insufficient functional support,and weak user engagement.This paper introduces the 4V marketing theory and constructs an analytical framework from four dimensions:differentiation,functionality,added value,and resonance.Two representative products,“Climbing Mathematics”and“Spark Mathematics,”are selected for a typical case comparison to identify their strengths and weaknesses in content design,service systems,and brand operation,and to extract transferable strategic elements.The study finds that the user-value-oriented strategy based on the 4V model can effectively address the core issues in the market promotion and user relationship building of leveled mathematics readers,providing practical paths and theoretical support for educational publishing institutions to achieve product innovation and brand upgrading in this niche field.
文摘A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional Gilbert mixer pair in commutating mode. A quadrature down-conversion mixer based on this topology is designed and optimized for an ultra high frequency RFID reader. Operating in the 915MHz ISM band, the presented quadrature mixer measures a conversion gain of 12.5dB,an IIP3 of 10dBm, an IIP2 of 58dBm, and an SSB noise figure of 17.6riB. The chip was fabricated in a 0. 18μm 1P6M RF CMOS process and consumes only 3mA of current from a 1.8V power supply.
基金the Science and Technology Commission of Shanghai Municipality(No.057062010)the EU BRIDGE Project(No.033546)~~
文摘A direct conversion receiver with optimized tolerance to local carrier interference is designed and implemented in a 0.18μm 1P6M mixed-signal CMOS process for a 900MHz RFID reader transceiver. A baseband amplifier with series feedback topology is proposed to achieve passive mixer buffering,baseband DC cancellation,and signal amplification simultaneously. The receiver has a measured input ldB compression point of - 4dBm and a sensitivity of - 70dBm when 10dB SNR for digital demodulation is required. The receiver is integrated in a reader transceiver chip and consumes 90mA from a 1.8V supply.
文摘Henry James' s first novella Daisy Miller brought him popularity and literary reputation after its publication. Through the application of reader theory and a close reading of the text, this article analyzes Daisy's strong personality and her misfortunes during her trip to Europe, and reveals how the novella highlights the conflict between American frankness and innocence and European sophistication. Besides, this article also elaborates on James' unique artistic skills—central consciousness and psychological analysis through his choice of narrative point of view, which contribute to the theme and the artistic charm of DaisyMiller.
文摘A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U.
