Bakanae disease,induced by the pathogen Fusarium fujikuroi,poses a significant stress on rice cultivation.However,the key resistance genes to this disease remain largely unknown.In this study,we reported that the plas...Bakanae disease,induced by the pathogen Fusarium fujikuroi,poses a significant stress on rice cultivation.However,the key resistance genes to this disease remain largely unknown.In this study,we reported that the plasma membrane intrinsic protein,OsPIP2;2,positively regulated to bakanae disease resistance in rice.展开更多
Bakanae is an emerging rice disease caused by the seed-and soil-borne pathogen Fusarium fujikuroi.It is becoming a more serious threat to sustainable rice production throughout rice-growing regions.Bakanae disease inf...Bakanae is an emerging rice disease caused by the seed-and soil-borne pathogen Fusarium fujikuroi.It is becoming a more serious threat to sustainable rice production throughout rice-growing regions.Bakanae disease infection is responsible for high yield losses ranging from 3%to 95%,and disease incidence varies based on the region and cultivars.Hence,understanding the nature of the pathogen,its pathogenicity,disease epidemiology,symptoms,host–pathogen interaction,and the role of secondary metabolites in the disease cycle will be helpful in the development of effective and sustainable management strategies.However,very few comprehensive studies have described the details of rice bakanae disease.Thus,in this review we summarize and discuss in detail the information available from 1898 to 2023 on various critical facets of bakanae disease,and provide perspectives on future research.展开更多
It is necessary to understand the bacterial populations associated with rice so as to provide more information and natural resources for effective management of major diseases in rice. A survey on screening and identi...It is necessary to understand the bacterial populations associated with rice so as to provide more information and natural resources for effective management of major diseases in rice. A survey on screening and identification of gram-positive bacteria was conducted during 1998 2004. Seven hundred and fifty-six rice samples were collected from Zhejiang, Jiangsu, Fujian and Yunnan Provinces, China. Over 1000 bacterial isolates were isolated and tested for colony morphology, pathogenicity, and some characteristics of bacteriology including Gram staining, fluorescent pigment on Kings medium B and microscopic observation for endospore. Together with five standard reference strains, 74 representative gram-positive bacterial isolates were confirmed by Biolog and gas chromatographic analysis of fatty acid methyl esters. Five bacterial species of Bacillus and other three genera were identified and isolates from Bacillus sublitis and Bacillus megaterium, exhibited the most effective inhibition against the pathogens of sheath blight and bakanae disease of rice. A few isolates from Bacillus pumilus and Bacillus megaterium showed weak virulent on rice together with some virulent isolates, risk should be considered when isolates from these species were screened for biocontrol agents.展开更多
After different treatments, germination of rice seeds and growth of young seedlings were observed, and the optimal seed soaking agent was selected by comparing seedling growth and incidence of bakanae disease in the f...After different treatments, germination of rice seeds and growth of young seedlings were observed, and the optimal seed soaking agent was selected by comparing seedling growth and incidence of bakanae disease in the fidd. The results showed that self-made powder （800 times） could promote indoor seed germina- tion and seedling growth, had no obvious inhibitory effect on the growth of seedlings in the field, and could reduce the incidence of bakanae disease of rice seed- lings, so it was the optimal treatment for controlling rice bakanae disease.展开更多
We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified fro...We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified from these isolates based on the method of Booth(1971) and other authors. Six kinds of medium wereused in the experiment. Color and growth rate ofcolonies as well as other characters were determinedfrom cultures grown on PSA. The results showedthat thirty one isolates were Fusarium moniliformevar. zhejiangensis, two isolates were Fusariumgramineum, one was Fusariurn oxysporum, and onewas Fusarium solani.展开更多
Bakanae disease,caused by Fusarium fujikuroi,poses a significant threat to rice production and has been observed in most rice-growing regions.The disease symptoms caused by different pathogens may vary,including elong...Bakanae disease,caused by Fusarium fujikuroi,poses a significant threat to rice production and has been observed in most rice-growing regions.The disease symptoms caused by different pathogens may vary,including elongated and weak stems,slender and yellow leaves,and dwarfism,as example.Bakanae disease is likely to cause necrosis of diseased seedlings,and it may cause a large area of infection in the field through the transmission of conidia.Therefore,early disease surveillance plays a crucial role in securing rice production.Traditional monitoring methods are both time-consuming and labor-intensive and cannot be broadly applied.In this study,a combination of hyperspectral imaging technology and deep learning algorithms were used to achieve in situ detection of rice seedlings infected with bakanae disease.Phenotypic data were obtained on the 9th,15th,and 21st day after rice infection to explore the physiological and biochemical performance,which helps to deepen the research on the disease mechanism.Hyperspectral data were obtained over these same periods of infection,and a deep learning model,named Rice Bakanae Disease-Visual Geometry Group(RBD-VGG),was established by leveraging hyperspectral imaging technology and deep learning algorithms.Based on this model,an average accuracy of 92.2%was achieved on the 21st day of infection.It also achieved an accuracy of 79.4%as early as the 9th day.Universal characteristic wavelengths were extracted to increase the feasibility of using portable spectral equipment for field surveillance.Collectively,the model offers an efficient and non-destructive surveillance methodology for monitoring bakanae disease,thereby providing an efficient avenue for disease prevention and control.展开更多
Rice bakanae disease(RBD)is a devastating plant disease caused by Fusarium fujikuroi.This study aimed to evaluate the potential of cyclobutrifuram,a novel succinate dehydrogenase inhibitor(SDHI),to control RBD,and det...Rice bakanae disease(RBD)is a devastating plant disease caused by Fusarium fujikuroi.This study aimed to evaluate the potential of cyclobutrifuram,a novel succinate dehydrogenase inhibitor(SDHI),to control RBD,and determine the risk and mechanism of resistance to cyclobutrifuram in F.fujikuroi.In vitro experiments showed that cyclobutrifuram significantly inhibited mycelial growth and spore germination,and altered the morphology of mycelia and conidia.Treatment with cyclobutrifuram signifcantly decreased mycotoxin production and increased cell membrane permeability in F.fujikuroi.The baseline sensitivity of 72 F.fujikuroi isolates to cyclobutrifuram was determined using mycelial growth and spore germination inhibition assays,which revealed EC50values of 0.0114-0.1304 and 0.0012-0.016μg mL^(-1),with mean EC50values of(0.0410±0.0470)and(0.0038±0.0015)μg mL^(-1),respectively.Pot experiments demonstrated that the protective effect of cyclobutrifluram against F.fujikuroi was more significant than that of phenamacril and azoxystrobin,indicating that cyclobutrifuram is a promising antifungal agent for the control of RBD.Six cyclobutrifuramresistant mutants of F.fujikuroi were obtained via fungicide adaptation.Moreover,these mutants exhibited weaker ftness than their parental isolate and positive cross-resistance with other SDHI fungicides,including pydifumetofen and penfufen;however,no cross-resistance was detected with other classes of fungicides,including phenamacril,fudioxonil,prochloraz,or azoxystrobin.These results indicated that the resistance risk of F.fujikuroi to cyclobutrifuram might be moderate.Sequencing analysis revealed that mutations,including H248D in Ff Sdh B,A83V in Ff Sdh C2,and S106F and E166K in Ff Sdh D,contributed to resistance,which was confrmed by molecular docking and homologous replacement experiments.The results suggest a high potential for cyclobutrifuram to control RBD and a moderate resistance risk of F.fujikuroi to cyclobutrifuram,which are meaningful fndings for the scientifc application of cyclobutrifuram.展开更多
The prevention and control effect of 35% thiamethoxam-prochloraz FS to rice thrips and rice bakanae disease and its influence on the quality of rice seedlings were studied. The results show that when 100 kg of rice se...The prevention and control effect of 35% thiamethoxam-prochloraz FS to rice thrips and rice bakanae disease and its influence on the quality of rice seedlings were studied. The results show that when 100 kg of rice seeds were coated with 200 g of 35% thiamethoxam-prochloraz FS,the control effects to rice thrips were 93.0% and 84.7% 15 and 20 days after sowing, and the control effect to rice bakanae disease was 90.8% 30 days after sowing. As 100 kg of rice seeds were coated with 250 g of 35% thiamethoxam-prochloraz FS, the control effects to rice thrips were 98.2% and 94.9% 15 and 20 days after sowing, and the control ef- fect to rice bakanae disease was 94.6% 30 days after sowing. 35% thiamethoxam- prochloraz FS is safe to rice seedlings and can effectively promote the growth of rice seedlings.展开更多
Diverse bacterial and fungal pathogens attack plants,causing biotic stress and severe yield losses globally.These losses are expected to become more serious as climate change improves conditions for many pathogens.The...Diverse bacterial and fungal pathogens attack plants,causing biotic stress and severe yield losses globally.These losses are expected to become more serious as climate change improves conditions for many pathogens.Therefore,identifying genes conferring broad-spectrum disease resistance and elucidating their underlying mechanisms provides important resources for plant breeding.WRKY transcription factors affect plant growth and stress responses.However,the functions of many WRKY proteins remain to be elucidated.Here,we demonstrated the role of rice(Oryza sativa)WRKY groupⅢtranscription factor OsWRKY65 in immunity.OsWRKY65 localized to the nucleus and acted as transcriptional repressor.Genetic and molecular functional analyses showed that OsWRKY65 increases resistance to the fungal pathogen Fusarium fujikuroi through downregulation of GA signaling and upregulation of JA signaling.Moreover,OsWRKY65 modulated the expression of the key genes that confer susceptibility or resistance to Xanthomonas oryzae pv.oryzae to enhance immunity against the pathogen.In particular,OsWRKY65directly bound to the promoter region of OsSWEET13 and repressed its expression.Taken together,our findings demonstrate that the OsWRKY65 enhances resistance to fungal and bacterial pathogens in rice.展开更多
基金supported by the‘Pioneer and Leading Goose’Research and Development Program of Zhejiang Province,China(Grant No.2023C04024)Hainan Province Science and Technology Special Fund,China(Grant No.ZDYF2023XDNY086)the Natural Science Foundation of Zhejiang Province,China(Grant No.LY22C130007).
文摘Bakanae disease,induced by the pathogen Fusarium fujikuroi,poses a significant stress on rice cultivation.However,the key resistance genes to this disease remain largely unknown.In this study,we reported that the plasma membrane intrinsic protein,OsPIP2;2,positively regulated to bakanae disease resistance in rice.
基金supported by the National Nuclear Energy Exploitation Program-Nuclear Irradiation for Crop Improvement and Insect Eradication and the International Atomic Energy Agency(IAEA)Coordination Research Project(No.D23020-23042),China.
文摘Bakanae is an emerging rice disease caused by the seed-and soil-borne pathogen Fusarium fujikuroi.It is becoming a more serious threat to sustainable rice production throughout rice-growing regions.Bakanae disease infection is responsible for high yield losses ranging from 3%to 95%,and disease incidence varies based on the region and cultivars.Hence,understanding the nature of the pathogen,its pathogenicity,disease epidemiology,symptoms,host–pathogen interaction,and the role of secondary metabolites in the disease cycle will be helpful in the development of effective and sustainable management strategies.However,very few comprehensive studies have described the details of rice bakanae disease.Thus,in this review we summarize and discuss in detail the information available from 1898 to 2023 on various critical facets of bakanae disease,and provide perspectives on future research.
基金the National Natural Science Foundation of China(30270891) National 863 Program(2002AA241261).
文摘It is necessary to understand the bacterial populations associated with rice so as to provide more information and natural resources for effective management of major diseases in rice. A survey on screening and identification of gram-positive bacteria was conducted during 1998 2004. Seven hundred and fifty-six rice samples were collected from Zhejiang, Jiangsu, Fujian and Yunnan Provinces, China. Over 1000 bacterial isolates were isolated and tested for colony morphology, pathogenicity, and some characteristics of bacteriology including Gram staining, fluorescent pigment on Kings medium B and microscopic observation for endospore. Together with five standard reference strains, 74 representative gram-positive bacterial isolates were confirmed by Biolog and gas chromatographic analysis of fatty acid methyl esters. Five bacterial species of Bacillus and other three genera were identified and isolates from Bacillus sublitis and Bacillus megaterium, exhibited the most effective inhibition against the pathogens of sheath blight and bakanae disease of rice. A few isolates from Bacillus pumilus and Bacillus megaterium showed weak virulent on rice together with some virulent isolates, risk should be considered when isolates from these species were screened for biocontrol agents.
文摘After different treatments, germination of rice seeds and growth of young seedlings were observed, and the optimal seed soaking agent was selected by comparing seedling growth and incidence of bakanae disease in the fidd. The results showed that self-made powder （800 times） could promote indoor seed germina- tion and seedling growth, had no obvious inhibitory effect on the growth of seedlings in the field, and could reduce the incidence of bakanae disease of rice seed- lings, so it was the optimal treatment for controlling rice bakanae disease.
文摘We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified from these isolates based on the method of Booth(1971) and other authors. Six kinds of medium wereused in the experiment. Color and growth rate ofcolonies as well as other characters were determinedfrom cultures grown on PSA. The results showedthat thirty one isolates were Fusarium moniliformevar. zhejiangensis, two isolates were Fusariumgramineum, one was Fusariurn oxysporum, and onewas Fusarium solani.
基金supported by National Key Research and Development Project(2023YFD2000103)Zhejiang province agricultural machinery research,manufacturing and application integration project(2023-YT-06)+2 种基金International S&T Cooperation Program of China(Grant No.2019YFE0103800)the National Key R&D Program of China(2021YFE0113700)the National Natural Science Foundation of China(32122074,U21A20219)。
文摘Bakanae disease,caused by Fusarium fujikuroi,poses a significant threat to rice production and has been observed in most rice-growing regions.The disease symptoms caused by different pathogens may vary,including elongated and weak stems,slender and yellow leaves,and dwarfism,as example.Bakanae disease is likely to cause necrosis of diseased seedlings,and it may cause a large area of infection in the field through the transmission of conidia.Therefore,early disease surveillance plays a crucial role in securing rice production.Traditional monitoring methods are both time-consuming and labor-intensive and cannot be broadly applied.In this study,a combination of hyperspectral imaging technology and deep learning algorithms were used to achieve in situ detection of rice seedlings infected with bakanae disease.Phenotypic data were obtained on the 9th,15th,and 21st day after rice infection to explore the physiological and biochemical performance,which helps to deepen the research on the disease mechanism.Hyperspectral data were obtained over these same periods of infection,and a deep learning model,named Rice Bakanae Disease-Visual Geometry Group(RBD-VGG),was established by leveraging hyperspectral imaging technology and deep learning algorithms.Based on this model,an average accuracy of 92.2%was achieved on the 21st day of infection.It also achieved an accuracy of 79.4%as early as the 9th day.Universal characteristic wavelengths were extracted to increase the feasibility of using portable spectral equipment for field surveillance.Collectively,the model offers an efficient and non-destructive surveillance methodology for monitoring bakanae disease,thereby providing an efficient avenue for disease prevention and control.
基金the National Natural Science Fundation of China(32302392)the Key Project of the Natural Science Foundation of Anhui Provincial Department of Education,China(2022AH050877 and 2023AH040129)+4 种基金the Programs for the Scientific Research Activities of Academic and Technical Leaders of Anhui Province,China(2020D251)the Development Fund for Talent Personnel of Anhui Agricultural University,China(rc342006 and rc342008)the University Synergy Innovation Program of Anhui Province,China(GXXT-2021-059)the Grants from Anhui Agricultural University,China(2020zd27)the Anhui Province Agricultural Eco-Environmental Protection and Quality Safety Industry Technology System,China。
文摘Rice bakanae disease(RBD)is a devastating plant disease caused by Fusarium fujikuroi.This study aimed to evaluate the potential of cyclobutrifuram,a novel succinate dehydrogenase inhibitor(SDHI),to control RBD,and determine the risk and mechanism of resistance to cyclobutrifuram in F.fujikuroi.In vitro experiments showed that cyclobutrifuram significantly inhibited mycelial growth and spore germination,and altered the morphology of mycelia and conidia.Treatment with cyclobutrifuram signifcantly decreased mycotoxin production and increased cell membrane permeability in F.fujikuroi.The baseline sensitivity of 72 F.fujikuroi isolates to cyclobutrifuram was determined using mycelial growth and spore germination inhibition assays,which revealed EC50values of 0.0114-0.1304 and 0.0012-0.016μg mL^(-1),with mean EC50values of(0.0410±0.0470)and(0.0038±0.0015)μg mL^(-1),respectively.Pot experiments demonstrated that the protective effect of cyclobutrifluram against F.fujikuroi was more significant than that of phenamacril and azoxystrobin,indicating that cyclobutrifuram is a promising antifungal agent for the control of RBD.Six cyclobutrifuramresistant mutants of F.fujikuroi were obtained via fungicide adaptation.Moreover,these mutants exhibited weaker ftness than their parental isolate and positive cross-resistance with other SDHI fungicides,including pydifumetofen and penfufen;however,no cross-resistance was detected with other classes of fungicides,including phenamacril,fudioxonil,prochloraz,or azoxystrobin.These results indicated that the resistance risk of F.fujikuroi to cyclobutrifuram might be moderate.Sequencing analysis revealed that mutations,including H248D in Ff Sdh B,A83V in Ff Sdh C2,and S106F and E166K in Ff Sdh D,contributed to resistance,which was confrmed by molecular docking and homologous replacement experiments.The results suggest a high potential for cyclobutrifuram to control RBD and a moderate resistance risk of F.fujikuroi to cyclobutrifuram,which are meaningful fndings for the scientifc application of cyclobutrifuram.
基金Supported by the Guiding Plan for Agricultural Science and Technology of Yancheng City(YKN2013018)~~
文摘The prevention and control effect of 35% thiamethoxam-prochloraz FS to rice thrips and rice bakanae disease and its influence on the quality of rice seedlings were studied. The results show that when 100 kg of rice seeds were coated with 200 g of 35% thiamethoxam-prochloraz FS,the control effects to rice thrips were 93.0% and 84.7% 15 and 20 days after sowing, and the control effect to rice bakanae disease was 90.8% 30 days after sowing. As 100 kg of rice seeds were coated with 250 g of 35% thiamethoxam-prochloraz FS, the control effects to rice thrips were 98.2% and 94.9% 15 and 20 days after sowing, and the control ef- fect to rice bakanae disease was 94.6% 30 days after sowing. 35% thiamethoxam- prochloraz FS is safe to rice seedlings and can effectively promote the growth of rice seedlings.
基金funded by Research Program for Agricultural Science and Technology Development(PJ01570601)and the Fellowship Program(PJ01661001 and PJ01570601)of the National Institute of Agricultural Sciences,Rural Development Administration,Republic of Korea。
文摘Diverse bacterial and fungal pathogens attack plants,causing biotic stress and severe yield losses globally.These losses are expected to become more serious as climate change improves conditions for many pathogens.Therefore,identifying genes conferring broad-spectrum disease resistance and elucidating their underlying mechanisms provides important resources for plant breeding.WRKY transcription factors affect plant growth and stress responses.However,the functions of many WRKY proteins remain to be elucidated.Here,we demonstrated the role of rice(Oryza sativa)WRKY groupⅢtranscription factor OsWRKY65 in immunity.OsWRKY65 localized to the nucleus and acted as transcriptional repressor.Genetic and molecular functional analyses showed that OsWRKY65 increases resistance to the fungal pathogen Fusarium fujikuroi through downregulation of GA signaling and upregulation of JA signaling.Moreover,OsWRKY65 modulated the expression of the key genes that confer susceptibility or resistance to Xanthomonas oryzae pv.oryzae to enhance immunity against the pathogen.In particular,OsWRKY65directly bound to the promoter region of OsSWEET13 and repressed its expression.Taken together,our findings demonstrate that the OsWRKY65 enhances resistance to fungal and bacterial pathogens in rice.
