In order to investigate the effects of interleukin-18 （IL-18） on airway inflammation and the Th1/Th2 cytokine balance in guinea pig asthmatic model as well as its possible mechanisms, the asthmatic model was establi...In order to investigate the effects of interleukin-18 （IL-18） on airway inflammation and the Th1/Th2 cytokine balance in guinea pig asthmatic model as well as its possible mechanisms, the asthmatic model was established by intraperitoneal injection of ovalbumin （OVA） and aerosol challenges into guinea pigs, and 30 treated animals were randomly divided into three groups of 10 animals in each groups, in which group A served as the asthmatic model, group B as the controls and group C as the group treated with IL-18. The counting and categorization of the inflammatory cells in bronchoalveolar lavage fluid （BALF） were performed by using light microscopy, and the contents of cytokines （ IFN-γ, IL- 2, IL-4 and IL-5） were assayed by means of the ELISA kit. The experimental results showed that the numbers of eosinophils （ESO） in BALF of group A, B and C were （97.70 ± 58.03） × 10^6/L, （11.68 × 9.95） × 10^6/L and （28.62 ± 10.46） × 10^6/L, respectively, in which the number of eosinophils in group A was significantly higher than those of group B and C. Also, the number of neutrophils in BALF of group A was even higher than those in group B and C. In addition, the contents of IFN-7 and IL-2 in group A were lower than those in group B and C, but the contents of IL-4 as well as IL-5 were rather higher than those in group B and C. It is evident from the above observations that IL-18 can effectively inhibit the asthmatic inflammatory cells in BALF with an imbalance of the Thl/Th2 cytokines, thus offer- ing the experimental basis for the clinical application of IL-18 in the prevention and treatment of asthma.展开更多
Objective:To explore the effects of natural moxibustion on inhibition of airway remodeling in bronchial asthmatic rats.Materials and Methods:Sprague Dawley rats were treated with ovalbumin to establish an asthma model...Objective:To explore the effects of natural moxibustion on inhibition of airway remodeling in bronchial asthmatic rats.Materials and Methods:Sprague Dawley rats were treated with ovalbumin to establish an asthma model.The ancient prescription of natural moxibustion,named Bai-Jie-Zi pulvis,was applied by pasting acupoints in combination with budesonide suspension inhalation to treat the asthmatic models for a consecutive period of 14 days.Histopathological changes were then observed and assessed.The cytokines involved in airway remodeling progression,namely matrix metalloproteinase 9(MMP-9)and transforming growth factor beta-1(TGF-β1)in lung tissue,were measured using immunohistochemistry and western blotting.The positive control group received treatment solely by inhalation of budesonide.Results:Combination therapy with budesonide suspension inhalation and natural moxibustion demonstrated significant improvements in inflammatory cell infiltration,bronchial mucus plug secretion,terminal bronchial obstruction,and edema in the lung tissue compared with the model(MOD)group(P<0.01,P<0.05,P<0.01,respectively).In addition,an enhanced effect in relieving branchial mucous plug secretion was observed in the BUD+BJZ group(P<0.01).Both MMP-9 and TGF-β1 expression were downregulated compared to the group treated solely with budesonide suspension inhalation(P<0.05,P<0.01,respectively).Conclusions:The combination therapy of natural moxibustion and budesonide inhalation demonstrated a synergistic effect in asthmatic rats,effectively inhibiting the airway remodeling process.This traditional external therapy exhibited efficacy in asthmatic rats and may potentially serve as a complementary approach in the treatment of asthma.展开更多
文摘In order to investigate the effects of interleukin-18 （IL-18） on airway inflammation and the Th1/Th2 cytokine balance in guinea pig asthmatic model as well as its possible mechanisms, the asthmatic model was established by intraperitoneal injection of ovalbumin （OVA） and aerosol challenges into guinea pigs, and 30 treated animals were randomly divided into three groups of 10 animals in each groups, in which group A served as the asthmatic model, group B as the controls and group C as the group treated with IL-18. The counting and categorization of the inflammatory cells in bronchoalveolar lavage fluid （BALF） were performed by using light microscopy, and the contents of cytokines （ IFN-γ, IL- 2, IL-4 and IL-5） were assayed by means of the ELISA kit. The experimental results showed that the numbers of eosinophils （ESO） in BALF of group A, B and C were （97.70 ± 58.03） × 10^6/L, （11.68 × 9.95） × 10^6/L and （28.62 ± 10.46） × 10^6/L, respectively, in which the number of eosinophils in group A was significantly higher than those of group B and C. Also, the number of neutrophils in BALF of group A was even higher than those in group B and C. In addition, the contents of IFN-7 and IL-2 in group A were lower than those in group B and C, but the contents of IL-4 as well as IL-5 were rather higher than those in group B and C. It is evident from the above observations that IL-18 can effectively inhibit the asthmatic inflammatory cells in BALF with an imbalance of the Thl/Th2 cytokines, thus offer- ing the experimental basis for the clinical application of IL-18 in the prevention and treatment of asthma.
基金supported by the National Natural Science Foundation of China(grant number:81874489)the Science and Technology Innovation Project of the Shanxi Provincial Education Department(grant number:2020 L0451)。
文摘Objective:To explore the effects of natural moxibustion on inhibition of airway remodeling in bronchial asthmatic rats.Materials and Methods:Sprague Dawley rats were treated with ovalbumin to establish an asthma model.The ancient prescription of natural moxibustion,named Bai-Jie-Zi pulvis,was applied by pasting acupoints in combination with budesonide suspension inhalation to treat the asthmatic models for a consecutive period of 14 days.Histopathological changes were then observed and assessed.The cytokines involved in airway remodeling progression,namely matrix metalloproteinase 9(MMP-9)and transforming growth factor beta-1(TGF-β1)in lung tissue,were measured using immunohistochemistry and western blotting.The positive control group received treatment solely by inhalation of budesonide.Results:Combination therapy with budesonide suspension inhalation and natural moxibustion demonstrated significant improvements in inflammatory cell infiltration,bronchial mucus plug secretion,terminal bronchial obstruction,and edema in the lung tissue compared with the model(MOD)group(P<0.01,P<0.05,P<0.01,respectively).In addition,an enhanced effect in relieving branchial mucous plug secretion was observed in the BUD+BJZ group(P<0.01).Both MMP-9 and TGF-β1 expression were downregulated compared to the group treated solely with budesonide suspension inhalation(P<0.05,P<0.01,respectively).Conclusions:The combination therapy of natural moxibustion and budesonide inhalation demonstrated a synergistic effect in asthmatic rats,effectively inhibiting the airway remodeling process.This traditional external therapy exhibited efficacy in asthmatic rats and may potentially serve as a complementary approach in the treatment of asthma.
