The vibration of a longitudinally moving rectangular plate submersed in an infinite liquid domain is studied analytically with the Rayleigh-Ritz method. The liquid is assumed to be incompressible, inviscid, and irrota...The vibration of a longitudinally moving rectangular plate submersed in an infinite liquid domain is studied analytically with the Rayleigh-Ritz method. The liquid is assumed to be incompressible, inviscid, and irrotational, and the velocity potential is used to describe the fluid velocity in the whole liquid field. The classical thin plate theory is used to derive mechanical energies of the traveling plate. As derivative of transverse displacement with respect to time in the compatibility condition equation exists, an exponential function is introduced to depict the dynamic deformation of the moving plate. It is shown that this exponential function works well with the Rayleigh- Ritz method. A convergence study shows a quick convergence speed for the immersed moving plate. Furthermore, the parametric study is carried out to demonstrate the effect of system parameters including the moving speed, the plate location, the liquid depth, the plate-liquid ratio, and the boundary condition. Results show that the above system parameters have significant influence on the vibration characteristics of the immersed moving plate. To extend the study, the method of added virtual mass incremental （AVMI） factor is used. The results show good agreement with those from the Rayleigh-Ritz method.展开更多
Alzheimer’s disease （AD） accounts for 60% to 80% of dementia cases and is the most common cause of dementia. In the past decade, studies have shown a close association between blood pressure and AD. It is found tha...Alzheimer’s disease （AD） accounts for 60% to 80% of dementia cases and is the most common cause of dementia. In the past decade, studies have shown a close association between blood pressure and AD. It is found that elevated blood pressure at midlife would in-crease the risk of dementia, including AD. However, there is nodefnitive conclusion about the relationship between elderly blood pressure and cognitive function. Abnormal pulse pressure may also increase the risk of dementia. The impact of antihypertensive drugs is inconclusive, and the mechanism of their protection of cognitive function is not clear.展开更多
Support Vector Regression (SVR) has already been proved to be one of the mostreferred and used machine learning technique in various fields. In this study, wehave addressed a predictive-cum-prescriptive analysis for f...Support Vector Regression (SVR) has already been proved to be one of the mostreferred and used machine learning technique in various fields. In this study, wehave addressed a predictive-cum-prescriptive analysis for finalizing fundallocations by the Government at center to the schemes under Central Plan andto the schemes under States and Union Territories Plan, with a goal to maximizeGross Value Added (GVA) at factor cost. Here, we have proposed a hybridmachine learning model comprising of OFS (Orthogonal Forward Selection),TLBO (Teaching Learning Based Optimization) and SVR for the prediction ofGVA at factor cost. In this model, referred as OFS–TLBO–SVR hybrid model,SVR is at the core of prediction mechanism, OFS is for identifying the relevantfeatures, and TLBO is to support in optimizing the free parameters of SVR andagain TLBO is used for optimizing the governable attributes of data.展开更多
Background In Alzheimer’s Disease(AD),about one-third of the risk genes identified by GWAS encode proteins that function predominantly in the endocytic pathways.Among them,the Ras and Rab Interactor 3(RIN3)is a guani...Background In Alzheimer’s Disease(AD),about one-third of the risk genes identified by GWAS encode proteins that function predominantly in the endocytic pathways.Among them,the Ras and Rab Interactor 3(RIN3)is a guanine nucleotide exchange factor(GEF)for the Rab5 small GTPase family and has been implicated to be a risk factor for both late onset AD(LOAD)and sporadic early onset AD(sEOAD).However,how RIN3 is linked to AD pathogenesis is currently undefined.Methods Quantitative PCR and immunoblotting were used to measure the RIN3 expression level in mouse brain tissues and cultured basal forebrain cholinergic neuron(BFCNs).Immunostaining was used to define subcellular localization of RIN3 and to visualize endosomal changes in cultured primary BFCNs and PC12 cells.Recombinant flag-tagged RIN3 protein was purified from HEK293T cells and was used to define RIN3-interactomes by mass spectrometry.RIN3-interacting partners were validated by co-immunoprecipitation,immunofluorescence and yeast two hybrid assays.Live imaging of primary neurons was used to examine axonal transport of amyloid precursor protein(APP)andβ-secretase 1(BACE1).Immunoblotting was used to detect protein expression,processing of APP and phosphorylated forms of Tau.Results We have shown that RIN3 mRNA level was significantly increased in the hippocampus and cortex of APP/PS1 mouse brain.Basal forebrain cholinergic neurons(BFCNs)cultured from E18 APP/PS1 mouse embryos also showed increased RIN3 expression accompanied by early endosome enlargement.In addition,via its proline rich domain,RIN3 recruited BIN1(bridging integrator 1)and CD2AP(CD2 associated protein),two other AD risk factors,to early endosomes.Interestingly,overexpression of RIN3 or CD2AP promoted APP cleavage to increase its carboxyl terminal fragments(CTFs)in PC12 cells.Upregulation of RIN3 or the neuronal isoform of BIN1 increased phosphorylated Tau level.Therefore,upregulation of RIN3 expression promoted accumulation of APP CTFs and increased phosphorylated Tau.These effects by RIN3 was rescued by the expression of a dominant negative Rab5(Rab5S34N)construct.Our study has thus pointed to that RIN3 acts through Rab5 to impact endosomal trafficking and signaling.Conclusion RIN3 is significantly upregulated and correlated with endosomal dysfunction in APP/PS1 mouse.Through interacting with BIN1 and CD2AP,increased RIN3 expression alters axonal trafficking and procession of APP.Together with our previous studies,our current work has thus provided important insights into the role of RIN3 in regulating endosomal signaling and trafficking.展开更多
基金Project supported by the National Natural Science Foundation of China(Nos.11302046 and 11672071)the Fundamental Research Funds for the Central Universities(No.N150504003)
文摘The vibration of a longitudinally moving rectangular plate submersed in an infinite liquid domain is studied analytically with the Rayleigh-Ritz method. The liquid is assumed to be incompressible, inviscid, and irrotational, and the velocity potential is used to describe the fluid velocity in the whole liquid field. The classical thin plate theory is used to derive mechanical energies of the traveling plate. As derivative of transverse displacement with respect to time in the compatibility condition equation exists, an exponential function is introduced to depict the dynamic deformation of the moving plate. It is shown that this exponential function works well with the Rayleigh- Ritz method. A convergence study shows a quick convergence speed for the immersed moving plate. Furthermore, the parametric study is carried out to demonstrate the effect of system parameters including the moving speed, the plate location, the liquid depth, the plate-liquid ratio, and the boundary condition. Results show that the above system parameters have significant influence on the vibration characteristics of the immersed moving plate. To extend the study, the method of added virtual mass incremental （AVMI） factor is used. The results show good agreement with those from the Rayleigh-Ritz method.
文摘Alzheimer’s disease （AD） accounts for 60% to 80% of dementia cases and is the most common cause of dementia. In the past decade, studies have shown a close association between blood pressure and AD. It is found that elevated blood pressure at midlife would in-crease the risk of dementia, including AD. However, there is nodefnitive conclusion about the relationship between elderly blood pressure and cognitive function. Abnormal pulse pressure may also increase the risk of dementia. The impact of antihypertensive drugs is inconclusive, and the mechanism of their protection of cognitive function is not clear.
文摘Support Vector Regression (SVR) has already been proved to be one of the mostreferred and used machine learning technique in various fields. In this study, wehave addressed a predictive-cum-prescriptive analysis for finalizing fundallocations by the Government at center to the schemes under Central Plan andto the schemes under States and Union Territories Plan, with a goal to maximizeGross Value Added (GVA) at factor cost. Here, we have proposed a hybridmachine learning model comprising of OFS (Orthogonal Forward Selection),TLBO (Teaching Learning Based Optimization) and SVR for the prediction ofGVA at factor cost. In this model, referred as OFS–TLBO–SVR hybrid model,SVR is at the core of prediction mechanism, OFS is for identifying the relevantfeatures, and TLBO is to support in optimizing the free parameters of SVR andagain TLBO is used for optimizing the governable attributes of data.
基金The project was financially supported by the National Natural Science Foundation of China[#81630029 and#81871005 for JD]the National Key R&D Program of China[#2016YFC13060000 for JD].UCSD ADRC P50 Pilot Grant(Wu).
文摘Background In Alzheimer’s Disease(AD),about one-third of the risk genes identified by GWAS encode proteins that function predominantly in the endocytic pathways.Among them,the Ras and Rab Interactor 3(RIN3)is a guanine nucleotide exchange factor(GEF)for the Rab5 small GTPase family and has been implicated to be a risk factor for both late onset AD(LOAD)and sporadic early onset AD(sEOAD).However,how RIN3 is linked to AD pathogenesis is currently undefined.Methods Quantitative PCR and immunoblotting were used to measure the RIN3 expression level in mouse brain tissues and cultured basal forebrain cholinergic neuron(BFCNs).Immunostaining was used to define subcellular localization of RIN3 and to visualize endosomal changes in cultured primary BFCNs and PC12 cells.Recombinant flag-tagged RIN3 protein was purified from HEK293T cells and was used to define RIN3-interactomes by mass spectrometry.RIN3-interacting partners were validated by co-immunoprecipitation,immunofluorescence and yeast two hybrid assays.Live imaging of primary neurons was used to examine axonal transport of amyloid precursor protein(APP)andβ-secretase 1(BACE1).Immunoblotting was used to detect protein expression,processing of APP and phosphorylated forms of Tau.Results We have shown that RIN3 mRNA level was significantly increased in the hippocampus and cortex of APP/PS1 mouse brain.Basal forebrain cholinergic neurons(BFCNs)cultured from E18 APP/PS1 mouse embryos also showed increased RIN3 expression accompanied by early endosome enlargement.In addition,via its proline rich domain,RIN3 recruited BIN1(bridging integrator 1)and CD2AP(CD2 associated protein),two other AD risk factors,to early endosomes.Interestingly,overexpression of RIN3 or CD2AP promoted APP cleavage to increase its carboxyl terminal fragments(CTFs)in PC12 cells.Upregulation of RIN3 or the neuronal isoform of BIN1 increased phosphorylated Tau level.Therefore,upregulation of RIN3 expression promoted accumulation of APP CTFs and increased phosphorylated Tau.These effects by RIN3 was rescued by the expression of a dominant negative Rab5(Rab5S34N)construct.Our study has thus pointed to that RIN3 acts through Rab5 to impact endosomal trafficking and signaling.Conclusion RIN3 is significantly upregulated and correlated with endosomal dysfunction in APP/PS1 mouse.Through interacting with BIN1 and CD2AP,increased RIN3 expression alters axonal trafficking and procession of APP.Together with our previous studies,our current work has thus provided important insights into the role of RIN3 in regulating endosomal signaling and trafficking.
