The Xp.2 region of the human X chromosome contains genes involved in a number ofinherited diseases and at least one locus that escapes X chromosome inactivation as well asabnormal methylation polymorphism.We isolated ...The Xp.2 region of the human X chromosome contains genes involved in a number ofinherited diseases and at least one locus that escapes X chromosome inactivation as well asabnormal methylation polymorphism.We isolated a series of yeast artificial chromosome(YAC)clones by hybridization screening with DNA probes localized within this region andassemblied them into a 4.3 Mb contig spanning from Xp11.21 to Xp11.23 by a combinationof Alu-PCR fingerprinting and DNA probe cross hybridization.On the basis of these over-lapping YAC clones,we have constructed the long-range restriction map of this interval andexactly placed some DNA markers.Four CpG-dense regions between ARAF1 and OATL2were identified by the long-range restriction mapping,which indicated the distribution ofgenes within this interval.It should assist in the future nucleic acid sequence analysis andnovel gene identification in this region.展开更多
基金the high Technology Research and Development Programme of chinaNational Natural Science Foundation of china
文摘The Xp.2 region of the human X chromosome contains genes involved in a number ofinherited diseases and at least one locus that escapes X chromosome inactivation as well asabnormal methylation polymorphism.We isolated a series of yeast artificial chromosome(YAC)clones by hybridization screening with DNA probes localized within this region andassemblied them into a 4.3 Mb contig spanning from Xp11.21 to Xp11.23 by a combinationof Alu-PCR fingerprinting and DNA probe cross hybridization.On the basis of these over-lapping YAC clones,we have constructed the long-range restriction map of this interval andexactly placed some DNA markers.Four CpG-dense regions between ARAF1 and OATL2were identified by the long-range restriction mapping,which indicated the distribution ofgenes within this interval.It should assist in the future nucleic acid sequence analysis andnovel gene identification in this region.
