A qualitative and quantitative test method of fingerprint of Wuji gastric floating sustained-release tablets was established. High performance liquid chromatography （HPLC） was adopted, using Agilent ZORBAX SB-C18 co...A qualitative and quantitative test method of fingerprint of Wuji gastric floating sustained-release tablets was established. High performance liquid chromatography （HPLC） was adopted, using Agilent ZORBAX SB-C18 column （250 mm×4.6 mm, 5 μm） as the chromatographic column, and acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution as the mobile phase in a gradient elution with the flow rate of 1.0 mL/min. Sample solution （10 μL） was injected and was tested at the wavelength of 225 nm for 75 min at the column temperature of 30 ℃, Fingerprint similarity software （2004A version） was used to conduct data analysis. A total of 11 batches of Wuji gastric floating sustained-release tablets were tested and analyzed with HPLC fingerprint. Seventeen common peaks were found and the similarity of the 11 batches of agents was greater than 0.9, indicating that the production process of the agent is stable and feasible. The method is operable and could effectively control the quality of Wuji gastric floating sustained-release tablets.展开更多
Wuji Town,which is located by the east of Mt.Hengshan(one of the Five Mountains in China),and the side of Mishui River(a principal east-west water transport in ancient central and southern regions of Hunan),once was a...Wuji Town,which is located by the east of Mt.Hengshan(one of the Five Mountains in China),and the side of Mishui River(a principal east-west water transport in ancient central and southern regions of Hunan),once was an important commercial town and a political,economic and cultural center in the lower and middle reaches of Mishui River due to the particular historical opportunities.Its tourist resources are characterized by "integrated natural scenery and ancient town,highly concentrated scenic spots,prosperous business in the history,row upon row of stores and workshops,numerous temples and ancestral halls,and various festivals".It was proposed that the protection and rural tourism development of Wuji Town should be based on consciousness raising,unification of concepts,scientific planning,application of integrated protection means,and development of regional clusters on the basis of famous mountain and city.展开更多
Background:To explore the effective components and mechanism of Classic ancient prescription Wuji pill on lung cancer(LC)with the help of network pharmacology and molecular docking technology.Methods:TCMSP database,Ch...Background:To explore the effective components and mechanism of Classic ancient prescription Wuji pill on lung cancer(LC)with the help of network pharmacology and molecular docking technology.Methods:TCMSP database,Chinese Pharmacopoeia(2020 Edition)and related literature were searched to identify the main chemical composition and targets of constituent drugs in Wuji pill,including Houpo(Magnoliae Officinalis Cortex),Huangqin(Scutellariae Radix),Badou(Crotonis Fructus),Huanglian(Coptidis Rhizoma)and Renshen(Ginseng Radix et Rhizoma).Meanwhile,Genecards,OMIM,TTD,Drugbank and PharmGkb databases were used to obtain the main targets of LC.The"chemical compound-target"network was constructed by using Cytoscape 3.8.2 software with the disease drug intersection gene as the research target.The PPI network was constructed by using the string database for protein interaction analysis,and the core target genes were sorted out.The Gene ontology go function enrichment analysis and KEGG signal pathway enrichment analysis were carried out based on the ClusterProfiler of R software.Finally,the Autodock Vina software was used to conduct molecular docking between the core target with high value and the key components.Results:A total of 60 active components,190 corresponding targets and 2516 GO and 163 KEGG pathways of Wuji pill were screened out,including 179 potential targets of LC.The core targets were ESR1,CDKN1A,TP53,NR3C1,MAPK1,AKT1,MAPK8,JUN,RELA,the core components were quercetin,kaempferol,β-sitosterol,stigmasterol,tetrahedron,protoopioid,etc,and the core pathways were PI3K-Akt,MAPK,IL-17,etc.Conclusion:This study preliminarily revealed the mechanism of action of Wuji pill on multi component-multi target-multi pathway of LC,which provided reference for the further clinical development and research of Wuji pill.展开更多
In An Introduction to Chinese Literature written by Liu Wuji,there are six translations of the passages in A Dream in Red Mansions,of which the Chinese version is the Zhiyanzhai version.Recently,it is doubted whether ...In An Introduction to Chinese Literature written by Liu Wuji,there are six translations of the passages in A Dream in Red Mansions,of which the Chinese version is the Zhiyanzhai version.Recently,it is doubted whether the six translations are all quoted.Through detailed analysis and literature review,this paper finds out that the translations of A Dream in Red Mansions are all translated by Liu Wuji,and the Chinese version is the Chengyi Version.展开更多
Disruption of the intestinal mucosal barrier caused by gut dysbiosis and metabolic imbalance is the underlying pathology of inflammatory bowel disease(IBD).Traditional Chinese medicine Wuji Wan(WJW)is commonly used to...Disruption of the intestinal mucosal barrier caused by gut dysbiosis and metabolic imbalance is the underlying pathology of inflammatory bowel disease(IBD).Traditional Chinese medicine Wuji Wan(WJW)is commonly used to treat digestive system disorders and showed therapeutic potential for IBD.In this interdisciplinary study,we aim to investigate the pharmacological effects of WJW against experimental colitis by combining functional metabolomics and gut-microbiota sequencing techniques.Treatment with WJW altered the profile of the intestinal microbiota and notably increased the abundance of Lactobacillus,thereby facilitating the conversion of tryptophan into indole-3-acetic acid(IAA)and indoleacrylic acid(IA).These indole derivatives activated the aryl hydrocarbon receptor(AhR)pathway,which reduced colonic inflammation and restored the expression of intestinal barrier proteins.Interestingly,the beneficial effects of WJW on gut barrier function improvement and tryptophan metabolism were disappeared in the absence of gut microbiota.Finally,pre-treatment with the AhR antagonist CH-223191 confirmed the essential role of IAA-mediated AhR activation in the therapeutic effects of WJW.Overall,WJW enhanced intestinal barrier function and reduced colonic inflammation in a murine colitis model by modulating LactobacilluseIAAeAhR signaling pathway.This study provides novel insights into colitis pathogenesis and presents an effective therapeutic and preventive approach against IBD.展开更多
Blood deficiency syndrome(BDS)refers to a pathological state with blood dysfunction and organ dystrophy in traditional Chinese medicine.Danggui Wuji granules(DWG)was developed from a formula containing Angelicae Sinen...Blood deficiency syndrome(BDS)refers to a pathological state with blood dysfunction and organ dystrophy in traditional Chinese medicine.Danggui Wuji granules(DWG)was developed from a formula containing Angelicae Sinensis Radix and Musculus et Os Galli Domestici.Herein,we investigated the mechanism of DWG in treating BDS by modulating gut microbiota.We found that DWG protected mice from BDS by elevating the levels of red blood cell count,hemoglobin,and hematocrit in peripheral blood and increasing the erythrocyte membrane Na+-K+-ATPase activity.Danggui Wuji granules changed the composition and metabolites of colonic flora.Notably,Lactobacillus,Muribaculaceae,and Alistipes were the main genera showing changes after DWG treatment.Our findings revealed that DWG presented a positive therapeutic effect on BDS in mice by regulating the gut microbiota and metabolites.The protective mechanism of DWG was associated with pathways such as metabolic pathways,biosynthesis of secondary metabolites,ABC transporters,ribosome,thyroid hormone synthesis,lysine degradation,galactose metabolism,tyrosine metabolism,etc.展开更多
基金The National Natural Science Foundation of China(Grant No.81460609)
文摘A qualitative and quantitative test method of fingerprint of Wuji gastric floating sustained-release tablets was established. High performance liquid chromatography （HPLC） was adopted, using Agilent ZORBAX SB-C18 column （250 mm×4.6 mm, 5 μm） as the chromatographic column, and acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution as the mobile phase in a gradient elution with the flow rate of 1.0 mL/min. Sample solution （10 μL） was injected and was tested at the wavelength of 225 nm for 75 min at the column temperature of 30 ℃, Fingerprint similarity software （2004A version） was used to conduct data analysis. A total of 11 batches of Wuji gastric floating sustained-release tablets were tested and analyzed with HPLC fingerprint. Seventeen common peaks were found and the similarity of the 11 batches of agents was greater than 0.9, indicating that the production process of the agent is stable and feasible. The method is operable and could effectively control the quality of Wuji gastric floating sustained-release tablets.
基金Supported by Major Social Science Bidding Project of Hunan Province (2010ZDA10)Key Social Science Planning Project of Hengyang City(2010B009)+4 种基金Provincial-level Competitive Course Construction Project of Hunan Universities and Colleges[No33(2006)]Social Science Planning Project of Hengyang City(2009D19)Scientific Foundation of Hengyang Normal University(08C01)Foundation of Key Social Science Research Base of Hengyang CityKey Disciplinary Program of Tourism Management at Hengyang Normal University~~
文摘Wuji Town,which is located by the east of Mt.Hengshan(one of the Five Mountains in China),and the side of Mishui River(a principal east-west water transport in ancient central and southern regions of Hunan),once was an important commercial town and a political,economic and cultural center in the lower and middle reaches of Mishui River due to the particular historical opportunities.Its tourist resources are characterized by "integrated natural scenery and ancient town,highly concentrated scenic spots,prosperous business in the history,row upon row of stores and workshops,numerous temples and ancestral halls,and various festivals".It was proposed that the protection and rural tourism development of Wuji Town should be based on consciousness raising,unification of concepts,scientific planning,application of integrated protection means,and development of regional clusters on the basis of famous mountain and city.
基金the General Program of National Natural Science Foundation of China(No.81873399)Major research project of oncology of scientific and technological innovation project of China Academy of Chinese Medical Sciences(No.CI2021A01805).
文摘Background:To explore the effective components and mechanism of Classic ancient prescription Wuji pill on lung cancer(LC)with the help of network pharmacology and molecular docking technology.Methods:TCMSP database,Chinese Pharmacopoeia(2020 Edition)and related literature were searched to identify the main chemical composition and targets of constituent drugs in Wuji pill,including Houpo(Magnoliae Officinalis Cortex),Huangqin(Scutellariae Radix),Badou(Crotonis Fructus),Huanglian(Coptidis Rhizoma)and Renshen(Ginseng Radix et Rhizoma).Meanwhile,Genecards,OMIM,TTD,Drugbank and PharmGkb databases were used to obtain the main targets of LC.The"chemical compound-target"network was constructed by using Cytoscape 3.8.2 software with the disease drug intersection gene as the research target.The PPI network was constructed by using the string database for protein interaction analysis,and the core target genes were sorted out.The Gene ontology go function enrichment analysis and KEGG signal pathway enrichment analysis were carried out based on the ClusterProfiler of R software.Finally,the Autodock Vina software was used to conduct molecular docking between the core target with high value and the key components.Results:A total of 60 active components,190 corresponding targets and 2516 GO and 163 KEGG pathways of Wuji pill were screened out,including 179 potential targets of LC.The core targets were ESR1,CDKN1A,TP53,NR3C1,MAPK1,AKT1,MAPK8,JUN,RELA,the core components were quercetin,kaempferol,β-sitosterol,stigmasterol,tetrahedron,protoopioid,etc,and the core pathways were PI3K-Akt,MAPK,IL-17,etc.Conclusion:This study preliminarily revealed the mechanism of action of Wuji pill on multi component-multi target-multi pathway of LC,which provided reference for the further clinical development and research of Wuji pill.
基金the staged achievement of 2013 China postdoctoral scientific funded program-On the English Translated Version of the Discourse Markers of the Four Classic Works of which Each Chapter Has a Caption(2013M540318)
文摘In An Introduction to Chinese Literature written by Liu Wuji,there are six translations of the passages in A Dream in Red Mansions,of which the Chinese version is the Zhiyanzhai version.Recently,it is doubted whether the six translations are all quoted.Through detailed analysis and literature review,this paper finds out that the translations of A Dream in Red Mansions are all translated by Liu Wuji,and the Chinese version is the Chengyi Version.
基金supported by the National Natural Scientific Foundation of China(82174059)Outstanding young and middleaged science and technology talents of Shaanxi Administration of Traditional Chinese Medicine(2023-ZQNY-004,China)+3 种基金the Natural Science Foundation of Shaanxi Province(2022SF-123,China)Innovative talent team introduction project in Xinzhou City(20220701,China)the Project of Shaanxi Administration of Traditional Chinese Medicine(No.2022-SLRH-YQ-001,China)the SKL-GRF Grant from State Key Laboratory of Environmental and Biological Analysis at Hong Kong Baptist University(SKLP_2425_P02,China).
文摘Disruption of the intestinal mucosal barrier caused by gut dysbiosis and metabolic imbalance is the underlying pathology of inflammatory bowel disease(IBD).Traditional Chinese medicine Wuji Wan(WJW)is commonly used to treat digestive system disorders and showed therapeutic potential for IBD.In this interdisciplinary study,we aim to investigate the pharmacological effects of WJW against experimental colitis by combining functional metabolomics and gut-microbiota sequencing techniques.Treatment with WJW altered the profile of the intestinal microbiota and notably increased the abundance of Lactobacillus,thereby facilitating the conversion of tryptophan into indole-3-acetic acid(IAA)and indoleacrylic acid(IA).These indole derivatives activated the aryl hydrocarbon receptor(AhR)pathway,which reduced colonic inflammation and restored the expression of intestinal barrier proteins.Interestingly,the beneficial effects of WJW on gut barrier function improvement and tryptophan metabolism were disappeared in the absence of gut microbiota.Finally,pre-treatment with the AhR antagonist CH-223191 confirmed the essential role of IAA-mediated AhR activation in the therapeutic effects of WJW.Overall,WJW enhanced intestinal barrier function and reduced colonic inflammation in a murine colitis model by modulating LactobacilluseIAAeAhR signaling pathway.This study provides novel insights into colitis pathogenesis and presents an effective therapeutic and preventive approach against IBD.
基金This work was supported by National Research and Development Project of China(grant no.2023YFF0724803)Scientific and technological innovation project of China Academy of Chinese Medical Sciences(grant no.CI2021B015)The Fundamental Research Funds for the Central Public Welfare Research Institutes(grant no.ZZ15-WT-04,ZZ15-WT-08).
文摘Blood deficiency syndrome(BDS)refers to a pathological state with blood dysfunction and organ dystrophy in traditional Chinese medicine.Danggui Wuji granules(DWG)was developed from a formula containing Angelicae Sinensis Radix and Musculus et Os Galli Domestici.Herein,we investigated the mechanism of DWG in treating BDS by modulating gut microbiota.We found that DWG protected mice from BDS by elevating the levels of red blood cell count,hemoglobin,and hematocrit in peripheral blood and increasing the erythrocyte membrane Na+-K+-ATPase activity.Danggui Wuji granules changed the composition and metabolites of colonic flora.Notably,Lactobacillus,Muribaculaceae,and Alistipes were the main genera showing changes after DWG treatment.Our findings revealed that DWG presented a positive therapeutic effect on BDS in mice by regulating the gut microbiota and metabolites.The protective mechanism of DWG was associated with pathways such as metabolic pathways,biosynthesis of secondary metabolites,ABC transporters,ribosome,thyroid hormone synthesis,lysine degradation,galactose metabolism,tyrosine metabolism,etc.
