The use of artificial intelligence(AI)in wound care has become a crucial instrument in improving the diagnosis,treatment,and management of chronic wounds.This study examines the several functions of AI in wound care,e...The use of artificial intelligence(AI)in wound care has become a crucial instrument in improving the diagnosis,treatment,and management of chronic wounds.This study examines the several functions of AI in wound care,emphasizing its ability to better wound evaluation,hasten healing,alleviate pain,and improve cost-effectiveness.AI-driven technology,including mobile applications and intelligent bandages,provides precise wound assessment,tissue analysis,and continuous monitoring,hence allowing for tailored treatment strategies and prompt intervention.Moreover,AI-driven algorithms forecast wound healing trajectories and find individuals susceptible to chronic wounds,allowing prompt preventative interventions.There are also practical issues with integrating it into clinical practice.Adding AI to current healthcare systems,like electronic medical records(EMRs),needs careful planning and teamwork to make sure that AI-driven insights are used well in real-time clinical decision-making.To make sure AI is used safely,legal frameworks need to be set up to provide clear rules for its use in healthcare.These frameworks should include openness,risk management,and data evaluation.Lastly,clinical validation and acceptance are still big problems.Many AI-powered tools don't have enough clinical validation and aren't widely available,which makes it hard to use them in everyday clinical practice.Solving these problems is important for getting the most out of AI in wound care and making sure it is used safely and effectively.The article combines current advancements and prospective trajectories in AI-assisted wound care,highlighting its revolutionary ability to revolutionize patient outcomes and healthcare delivery.展开更多
Chronic,non-healing wounds represent a significant challenge for healthcare systems worldwide,often requiring significant human and financial resources.Chronic wounds arise from the complex interplay of underlying com...Chronic,non-healing wounds represent a significant challenge for healthcare systems worldwide,often requiring significant human and financial resources.Chronic wounds arise from the complex interplay of underlying comorbidities,such as diabetes or vascular diseases,lifestyle factors,and genetic risk profiles which may predispose extremities to local ischemia.Injuries are further exacerbated by bacterial colonization and the formation of biofilms.Infection,consequently,perpetuates a chronic inflammatory microenvironment,preventing the progression and completion of normal wound healing.The current standard of care(SOC)for chronic wounds involves surgical debridement along with localized wound irrigation,which requires inpatient care under general anesthesia.This could be followed by,if necessary,defect coverage via a reconstructive ladder utilizing wound debridement along with skin graft,local,or free flap techniques once the wound conditions are stabilized and adequate blood supply is restored.To promote physiological wound healing,a variety of approaches have been subjected to translational research.Beyond conventional wound healing drugs and devices that currently supplement treatments,cellular and immunotherapies have emerged as promising therapeutics that can behave as tailored therapies with cell-or molecule-specific wound healing properties.However,in contrast to the clinical omnipresence of chronic wound healing disorders,there remains a shortage of studies condensing the current body of evidence on cellular therapies and immunotherapies for chronic wounds.This review provides a comprehensive exploration of current therapies,experimental approaches,and translational studies,offering insights into their efficacy and limitations.Ultimately,we hope this line of research may serve as an evidence-based foundation to guide further experimental and translational approaches and optimize patient care long-term.展开更多
Refractory wounds cause significant harm to the health of patients and the most common treatments in clinical practice are surgical debridement and wound dressings.However,certain challenges,including surgical difficu...Refractory wounds cause significant harm to the health of patients and the most common treatments in clinical practice are surgical debridement and wound dressings.However,certain challenges,including surgical difficulty,lengthy recovery times,and a high recurrence rate persist.Conductive hydrogel dressings with combined monitoring and therapeutic properties have strong advantages in promoting wound healing due to the stimulation of endogenous current on wounds and are the focus of recent advancements.Therefore,this review introduces the mechanism of conductive hydrogel used for wound monitoring and healing,the materials selection of conductive hydrogel dressings used for wound monitoring,focuses on the conductive hydrogel sensor to monitor the output categories of wound status signals,proving invaluable for non-invasive,real-time evaluation of wound condition to encourage wound healing.Notably,the research of artificial intelligence(AI)model based on sensor derived data to predict the wound healing state,AI makes use of this abundant data set to forecast and optimize the trajectory of tissue regeneration and assess the stage of wound healing.Finally,refractory wounds including pressure ulcers,diabetes ulcers and articular wounds,and the corresponding wound monitoring and healing process are discussed in detail.This manuscript supports the growth of clinically linked disciplines and offers motivation to researchers working in the multidisciplinary field of conductive hydrogel dressings.展开更多
BACKGROUND Diabetic foot ulcers(DFUs)are a significant challenge in diabetic care,and the efficacy of negative pressure wound therapy(NPWT)in treating them remains a subject of continuous investigation.AIM To provide ...BACKGROUND Diabetic foot ulcers(DFUs)are a significant challenge in diabetic care,and the efficacy of negative pressure wound therapy(NPWT)in treating them remains a subject of continuous investigation.AIM To provide a comprehensive meta-analysis of the role of NPWT in the manage-ment of DFUs.METHODS A systematic review was performed based on Preferred Reporting Items for Sys-tematic Reviews and Meta-Analyses guidelines,searching databases like PubMed,Embase,Web of Science,and the Cochrane Library.Randomized clinical trials(RCTs)were included to compare NPWT to other dressings for DFUs.Outcomes measured were wound healing time and rate,granulation tissue formation time,amputation rate,and adverse events.Study quality was evaluated using Coch-rane's risk of bias tool.Analyses utilizedχ2,I2,fixed or random-effects models via Stata v17.RESULTS Of the 1101 identified articles,9 RCTs were selected for meta-analysis.Studies spanned from 2005 to 2020 and originated from countries including the United States,Chile,Pakistan,Italy,India,and Germany.Meta-analysis demonstrated a significant improvement in wound healing rate[risk ratio(RR)=1.46,95%CI:1.22-1.76,P<0.01]and a reduction in amputation rate(RR=0.69,95%CI:0.50-0.96,P=0.006)with NPWT.Furthermore,the time for granulation tissue formation was significantly reduced by an average of 19.54 days.However,the incidence of adverse events did not significantly differ between NPWT and control treatments.CONCLUSION NPWT significantly improves wound healing rates and reduces amputation rates in DFUs.It also hastens the formation of granulation tissue.However,the therapy does not significantly alter the risk of adverse events compared to alternate treatments.展开更多
The healing of diabetic wounds poses a significant healthcare burden due to persistent inflammation,M1 macrophage aggregation,and high glucose levels in the microenvironment.Previous studies have demonstrated that imm...The healing of diabetic wounds poses a significant healthcare burden due to persistent inflammation,M1 macrophage aggregation,and high glucose levels in the microenvironment.Previous studies have demonstrated that immunomodulatory hydrogel dressings can facilitate diabetic wound healing.However,current immunomodulatory hydrogels require costly and complex treatments such as cell therapy and cytokines.Herein,a hierarchical hydrogel dressing with continuous biochemical gradient based on glycyrrhizic acid(GA) was constructed to modulate immunomodulatory processes in diabetic wounds.The hydrogels present many desirable features,such as tunable mechanical properties,broad antibacterial ability,outstanding conductive,transparent,and self-adhesive properties.The resultant hydrogel can promote diabetic wound healing by preventing bacterial infection,promoting macrophage polarization,improving the inflammatory microenvironment,and inducing angiogenesis and neurogenesis.Furthermore,electrical stimulation(ES) can further promote the healing of chronic diabetic wounds,providing valuable guidance for relevant clinical practice.展开更多
Necrotizing fasciitis(NF)is a rapidly progressing,life-threatening soft tissue infection,with upper limb NF posing a particularly serious threat to patient survival and quality of life.Negative pressure wound therapy(...Necrotizing fasciitis(NF)is a rapidly progressing,life-threatening soft tissue infection,with upper limb NF posing a particularly serious threat to patient survival and quality of life.Negative pressure wound therapy(NPWT)has shown considerable advantages in accelerating wound healing and mitigating functional impairment.A retrospective study by Lipatov et al.demonstrated that NPWT significantly reduced the time needed for wound closure preparation while enhancing the success rate of local repair.Despite its benefits,certain limitations highlight the need for further optimization.This paper investigates the potential for personalized dynamic regulation of NPWT,its integration with adjunctive therapies,and the role of multidisciplinary collaboration.Furthermore,it explores the incorporation of advanced technologies such as artificial intelligence,imaging modalities,and biomaterials,presenting novel pathways for the personalized management and global standardization of NF treatment.展开更多
Rapidly-advancing microneedle-based bioelectronics integrated with electrical stimulation(ES)therapy exhibit significant potential for improving chronic wound management.Herein,bio-inspired by the serrated structure o...Rapidly-advancing microneedle-based bioelectronics integrated with electrical stimulation(ES)therapy exhibit significant potential for improving chronic wound management.Herein,bio-inspired by the serrated structure of bee-stingers,we developed a temperature-sensitive,two-stage microneedle-based electro active platform(GP-PPy/PLA-MN)featuring rivet-like micros tructures that integrates intelligent,precise drug-releasing,ES-transmission,and real-time wound-assessment monitoring for comprehensive chronic wound-management and diagnostic therapy.The bionic-design mechanically anchors the microneedle beneath the skin's dermis,while GP-PPy/PLA-MN demonstrates versatile therapeutic characteristics,including outstanding biocompatibility,antimicrobial properties,and antimigratory origins.The GP-PPy/PLA-MN enables the sustained release of insulin at body temperature for up to24 hours through the poly-N-isopropyl acrylamide grafted amidated-gelatin-based thermo-sensitive hydrogel at the needle-tip,thereby providing long-term stable blood glucose control.GP-PPy/PLA-MN indicates its potential as a novel bioelectronics-based patch to record the temperature and humidity during the wound-healing process,realizing significant wound diagnosis and real-time wound assessment,and fundamentally facilitating the therapeutic efficacy by supplying solid data to protect the clinical practice.Extensive in vitro and in vivo studies have demonstrated that GP-PPy/PLA-MN can provide effective ES and sustained drug release,thereby promoting chronic wound healing and increasing the wound healing rate by 20%compared to the control group after 14 days of treatment.This innovative approach combines bioelectronics with intelligent drug delivery and microneedling technology to effectively address the critical challenges of chronic wound management,offering promising prospects for precision diagnostics and therapeutic interventions.展开更多
BACKGROUND Mesenchymal stem cells,found in various tissues,possess significant healing and immunomodulatory properties,influencing macrophage polarization,which is essential for wound repair.However,chronic wounds pre...BACKGROUND Mesenchymal stem cells,found in various tissues,possess significant healing and immunomodulatory properties,influencing macrophage polarization,which is essential for wound repair.However,chronic wounds present significant therapeutic challenges,requiring novel strategies to improve healing outcomes.AIM To investigate the potential of fetal dermal mesenchymal stem cells(FDMSCs)in enhancing wound healing through modulation of macrophage polarization,specifically by promoting the M2 phenotype to address inflammatory responses in chronic wounds.METHODS FDMSCs were isolated from BalB/C mice and co-cultured with RAW264.7 macrophages to assess their effects on macrophage polarization.Flow cytometry,quantitative reverse transcriptase polymerase chain reaction,and histological analyses were employed to evaluate shifts in macrophage phenotype and wound healing in a mouse model.Statistical analysis was performed using GraphPad Prism.RESULTS FDMSCs induced macrophage polarization from the M1 to M2 phenotype,as demonstrated by a reduction in proinflammatory markers(inducible nitric oxide synthase,interleukin-6)and an increase in anti-inflammatory markers[mannose receptor(CD206),arginase-1]in co-cultured RAW264.7 macrophages.These shifts were confirmed by flow cytometry.In an acute skin wound model,FDMSC-treated mice exhibited faster wound healing,enhanced collagen deposition,and improved vascular regeneration compared to controls.Significantly higher expression of arginase-1 further indicated an enriched M2 macrophage environment.CONCLUSION FDMSCs effectively modulate macrophage polarization from M1 to M2,reduce inflammation,and enhance tissue repair,demonstrating their potential as an immunomodulatory strategy in wound healing.These findings highlight the promising therapeutic application of FDMSCs in managing chronic wounds.展开更多
BACKGROUND Diabetic foot ulcers(DFUs)present a significant clinical challenge due to their high prevalence and profound impact on morbidity.Ultrasound-assisted wound debridement(UAWD)has emerged as a potential therape...BACKGROUND Diabetic foot ulcers(DFUs)present a significant clinical challenge due to their high prevalence and profound impact on morbidity.Ultrasound-assisted wound debridement(UAWD)has emerged as a potential therapeutic modality to improve healing outcomes in DFU management.AIM To evaluate the efficacy of UAWD in treating DFUs on wound closure rates,treatment duration,and quality of life outcomes.METHODS This systematic review and meta-analysis followed PRISMA guidelines,systematically searching PubMed,Embase,Web of Science,and the Cochrane Library with no date restrictions.Randomized controlled trials(RCTs)that evaluated the efficacy of UAWD in DFU treatment were included.Data were independently extracted by two reviewers,with discrepancies resolved through consensus or third-party consultation.The risk of bias was assessed using the Cochrane tool.χ2 and I2 statistics assessed heterogeneity,informing the use of fixed or random-effects models for meta-analysis,supplemented by sensitivity analysis and publication bias assessment through funnel plots and Egger's test.RESULTS From 1255 articles,seven RCTs met the inclusion criteria.The studies demonstrated that UAWD significantly reduced DFU healing time(standardized mean difference=-0.78,95%CI:-0.97 to-0.60,P<0.001)and increased healing rates(odds ratio=9.96,95%CI:5.99 to 16.56,P<0.001)compared to standard care.Sensitivity analysis confirmed the stability of these results,and no significant publication bias was detected.CONCLUSION UAWD is a promising adjunctive treatment for DFUs,significantly reducing healing times and increasing healing rates.These findings advocate for the integration of UAWD into standard DFU care protocols.展开更多
Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages,ultimately impairing its normal physiological function.A...Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages,ultimately impairing its normal physiological function.Accumulating evidence underscores the potential of targeted modulation of mechanical cues to enhance skin regeneration,promoting scarless repair by influencing the extracellular microenvironment and driving the phenotypic transitions.The field of skin repair and skin appendage regeneration has witnessed remarkable advancements in the utilization of biomaterials with distinct physical properties.However,a comprehensive understanding of the underlying mechanisms remains somewhat elusive,limiting the broader application of these innovations.In this review,we present two promising biomaterial-based mechanical approaches aimed at bolstering the regenerative capacity of compromised skin.The first approach involves leveraging biomaterials with specific biophysical properties to create an optimal scarless environment that supports cellular activities essential for regeneration.The second approach centers on harnessing mechanical forces exerted by biomaterials to enhance cellular plasticity,facilitating efficient cellular reprogramming and,consequently,promoting the regeneration of skin appendages.In summary,the manipulation of mechanical cues using biomaterial-based strategies holds significant promise as a supplementary approach for achieving scarless wound healing,coupled with the restoration of multiple skin appendage functions.展开更多
Wearable sensing systems have been designed to monitor health conditions in real-time by detecting analytes in human biofluids.Wound diagnosis remains challenging,necessitating suitable materials for high-performance ...Wearable sensing systems have been designed to monitor health conditions in real-time by detecting analytes in human biofluids.Wound diagnosis remains challenging,necessitating suitable materials for high-performance wearable sensors to offer prompt feedback.Existing devices have limitations in measuring pH and the concentration of pH-dependent electroactive species simultaneously,which is crucial for obtaining a comprehensive understanding of wound status and optimizing biosensors.Therefore,improving materials and analysis system accuracy is essential.This article introduces the first example of a flexible array capable of detecting pyocyanin,a bacterial virulence factor,while correcting dynamic pH fluctuations.We demonstrate that this combined sensor enhances accuracy by mitigating the impact of pH variability on pyocyanin sensor response.Customized screen-printable inks were developed to enhance analytical performance.The analytical performances of two sensitive sensor systems(i.e.,fully-printed porous graphene/multiwalled carbon nanotube(CNT)and polyaniline/CNT composites for pyocyanin and pH sensors)are evaluated.Partial least square regression is employed to analyze nonzero-order data arrays from square wave voltammetric and potentiometric measurements of pyocyanin and pH sensors to establish a predictive model for pyocyanin concentration in complex fluids.This sensitive and effective strategy shows potential for personalized applications due to its affordability,ease of use,and ability to adjust for dynamic pH changes.展开更多
Abnormal wound scarring often leads to functional impairments and cosmetic deformities,primarily driven by the prolonged activation of the TGF-β/Smad signaling pathway.Addressing this challenge,we developed a biomime...Abnormal wound scarring often leads to functional impairments and cosmetic deformities,primarily driven by the prolonged activation of the TGF-β/Smad signaling pathway.Addressing this challenge,we developed a biomimetic scaffold aimed at facilitating rapid and scarless wound healing.This highly in-tegrated 3D-printed dermal scaffold comprised modified recombinant human type III collagen(rhCOLIII-MA),gelatin methacrylate(GelMA),and liposomes encapsulating SB431542 to target TGF-β1(Lip@SB).The rhCOLIII-MA/GelMA(CG)scaffold retained inherent biomaterial characteristics,exhibited tailored physicochemical properties,and demonstrated favorable biocompatibility.Moreover,the Lip@SB-loaded CG scaffold(CGL)effectively promoted in vitro wound healing,while enabling controlled release of SB431542 to inhibit pathological collagen deposition.In a full-thickness skin defect rat model,the CGL dermal scaffold combined with split-thickness skin graft(STSG)minimized scar contraction,stimulated functional neovascularization,and enhanced graft aesthetics comparable to normal skin.Remarkably,the performance of the CGL scaffold surpassed that of commercially available anti-scarring alternatives.This innovative strategy presents a straightforward approach toward scarless skin regeneration and holds promise in alleviating the prolonged,painful postoperative rehabilitation.展开更多
Histopathological analysis of chronic wounds is crucial for clinicians to accurately assess wound healing progress and detect potential malignancy.However,traditional pathological tissue sections require specific stai...Histopathological analysis of chronic wounds is crucial for clinicians to accurately assess wound healing progress and detect potential malignancy.However,traditional pathological tissue sections require specific staining procedures involving carcinogenic chemicals.This study proposes an interdisciplinary approach merging materials science,medicine,and artificial intelligence(AI)to develop a virtual staining technique and intelligent evaluation model based on deep learning for chronic wound tissue pathology.This innovation aims to enhance clinical diagnosis and treatment by offering personalized AI-driven therapeutic strategies.By establishing a mouse model of chronic wounds and using a series of hydrogel wound dressings,tissue pathology sections were periodically collected for manual staining and healing assessment.We focused on leveraging the pix2pix image translation framework within deep learning networks.Through CNN models implemented in Python using PyTorch,our study involves learning and feature extraction for region segmentation of pathological slides.Comparative analysis between virtual staining and manual staining results,along with healing diagnosis conclusions,aims to optimize AI models.Ultimately,this approach integrates new metrics such as image recognition,quantitative analysis,and digital diagnostics to formulate an intelligent wound assessment model,facilitating smart monitoring and personalized treatment of wounds.In blind evaluation by pathologists,minimal disparities were found between virtual and conventional histologically stained images of murine wound tissue.The evaluation used pathologists’average scores on real stained images as a benchmark.The scores for virtual stained images were 71.1%for cellular features,75.4%for tissue structures,and 77.8%for overall assessment.Metrics such as PSNR(20.265)and SSIM(0.634)demonstrated our algorithms’superior performance over existing networks.Eight pathological features such as epidermis,hair follicles,and granulation tissue can be accurately identified,and the images were found to be more faithful to the actual tissue feature distribution when compared to manually annotated data.展开更多
After brain damage,regenerative angiogenesis and neurogenesis have been shown to occur simultaneously in mammals,suggesting a close link between these processes.However,the mechanisms by which these processes interact...After brain damage,regenerative angiogenesis and neurogenesis have been shown to occur simultaneously in mammals,suggesting a close link between these processes.However,the mechanisms by which these processes interact are not well understood.In this work,we aimed to study the correlation between angiogenesis and neurogenesis after a telencephalic stab wound injury.To this end,we used zebrafish as a relevant model of neuroplasticity and brain repair mechanisms.First,using the Tg(fli1:EGFP×mpeg1.1:mCherry)zebrafish line,which enables visualization of blood vessels and microglia respectively,we analyzed regenerative angiogenesis from 1 to 21 days post-lesion.In parallel,we monitored brain cell proliferation in neurogenic niches localized in the ventricular zone by using immunohistochemistry.We found that after brain damage,the blood vessel area and width as well as expression of the fli1 transgene and vascular endothelial growth factor(vegfaa and vegfbb)were increased.At the same time,neural stem cell proliferation was also increased,peaking between 3 and 5 days post-lesion in a manner similar to angiogenesis,along with the recruitment of microglia.Then,through pharmacological manipulation by injecting an anti-angiogenic drug(Tivozanib)or Vegf at the lesion site,we demonstrated that blocking or activating Vegf signaling modulated both angiogenic and neurogenic processes,as well as microglial recruitment.Finally,we showed that inhibition of microglia by clodronate-containing liposome injection or dexamethasone treatment impairs regenerative neurogenesis,as previously described,as well as injury-induced angiogenesis.In conclusion,we have described regenerative angiogenesis in zebrafish for the first time and have highlighted the role of inflammation in this process.In addition,we have shown that both angiogenesis and neurogenesis are involved in brain repair and that microglia and inflammation-dependent mechanisms activated by Vegf signaling are important contributors to these processes.This study paves the way for a better understanding of the effect of Vegf on microglia and for studies aimed at promoting angiogenesis to improve brain plasticity after brain injury.展开更多
The treatment of skin wounds,especially chronic wounds,remains a critical clinical challenge and places a heavy burden on patients and healthcare systems.In recent years,the engineering strategy of using biomaterial-a...The treatment of skin wounds,especially chronic wounds,remains a critical clinical challenge and places a heavy burden on patients and healthcare systems.In recent years,the engineering strategy of using biomaterial-assisted exosomes has emerged as a powerful tool for skin repair.Compared to treatments such as debridement and regular dressing changes,the design of biomaterial-assisted exosomes not only maintains the bioactivity of exosomes at the wound site but also provides an appropriate microenvironment for the repair of complex tissues,thereby accelerating wound healing.This review systematically introduces the general characteristics of exosomes and their functions in skin wound healing,highlights recent advances in classification of natural exosomes and engineering methods which enriching their functions in intercellular communication.Then,various emerging and innovative approaches based on biomaterials delivery of exosomes are comprehensively discussed.The review seeks to bring an in-depth understanding of bioactive dressings based on exosomes therapeutic strategies,aiming to facilitate new clinical application value.展开更多
Wound management remains significant challenges,encompassing numerous risks such as infection,antibiotic resistance,tissue adhesion,ischemia,hypoxia,and inflammatory responses.These complications are particularly acut...Wound management remains significant challenges,encompassing numerous risks such as infection,antibiotic resistance,tissue adhesion,ischemia,hypoxia,and inflammatory responses.These complications are particularly acute in cases of bacterial infection,where conventional treatment modalities may fail to provide adequate efficacy.To address these challenges,this study introduces phytic acid(PA)@ε-polylysine(Ply)@curcumin(Cur)@Zn@Gauze(PPCZ@Gauze),an innovative multifunctional gauze tailored to enhance wound healing.PPCZ@Gauze is designed and prepared using biosafe materials(phytic acid,ε-polylysine,curcumin,and zinc ions)through advanced surface modification techniques.Both in vitro and in vivo experiments demonstrate remarkable antibacterial properties,reduced tissue adhesion,and accelerated wound healing capabilities of PPCZ@Gauze compared to conventional medical gauze.Notably,PPCZ@Gauze also exhibits ease of preparation,cost-effectiveness,and scalability for large-scale production.Overall,this innovative multifunctional gauze offers a promising solution for managing bacterial infection wounds,leading to enhanced therapeutic outcomes and patient care.展开更多
The management of chronic wounds remains a substantial challenge for healthcare providers. Inadequate wound care can result in serious complications, including infection, which may ultimately lead to amputation or eve...The management of chronic wounds remains a substantial challenge for healthcare providers. Inadequate wound care can result in serious complications, including infection, which may ultimately lead to amputation or even death. While traditional excipients exhibit some efficacy in promoting wound healing, they are not sufficiently effective in preventing wound infections. As an antimicrobial metal, copper has a long history in the antimicrobial field, and at the same time, wound auxiliaries with copper ions have also been used in the treatment of chronic wounds. To address the limitations of conventional wound dressings, including insufficient antimicrobial properties and limited capacity to promote wound healing, this study introduces a highly adhesive hydrogel with superior mechanical stability for non-invasive wound treatment. The hydrogel was composed of carboxymethyl chitosan, tannic acid and copper ions. The tannic acid solution was subjected to dropwise addition of CuCl2 solution to produce precipitation, and tannic acid/copper ions (TA/Cu2+) composite nanoparticles were prepared. Through topological adhesion, the CMCS with pH sensitivity has the ability to establish adhesive connections with a wide range of materials. The benefits of CMCS/TA/Cu2+ hydrogel, as a kind of wound closure and repair material, include efficient wound closure, and resistance against bacterial invasion while maintaining cleanliness. Additionally, it exhibits excellent tensile and mechanical stability that can facilitate effective closure and repair in dynamic areas like joint wounds. This promising hydrogel adhesive has demonstrated potential as a material for wound closure and restoration.展开更多
Bacterial infection,insufficient angiogenesis,and oxidative damage are generally regarded as key issues that impede wound healing,making it necessary to prepare new biomaterials to simultaneously address these problem...Bacterial infection,insufficient angiogenesis,and oxidative damage are generally regarded as key issues that impede wound healing,making it necessary to prepare new biomaterials to simultaneously address these problems.In this work,monodispersed CeO_(2)@CuS nanocomposites(NCs)were successfully prepared with tannin(TA)as the reductant and linker.Due to abundant oxygen vacancies in CeO_(2)and the polyphenolic structure of TA,the TA-CeO_(2)@CuS NCs exhibited a remarkable antioxidant ability to scavenge excessive reactive oxygen species(ROS),which would likely induce serious inflammation.In addition,the TA-CeO_(2)@CuS NCs demonstrated excellent antibacterial capability with near-infrared ray(NIR)irradiation,and the released copper ions could promote the regeneration of blood vessels.These synergistic effects indicated that the synthesized TA-CeO_(2)@CuS NCs could serve as a promising biomaterial for multimodal wound therapy.展开更多
Diabetes wounds present a complex microenvironment characterized by persistent inflammation,elevated reactive oxygen species(ROS),and compromised vascular conditions,all of which contribute to delayed or incomplete he...Diabetes wounds present a complex microenvironment characterized by persistent inflammation,elevated reactive oxygen species(ROS),and compromised vascular conditions,all of which contribute to delayed or incomplete healing.Although advances in wound care have been made,effective strategies to address these multifaceted challenges remain limited.In this study,we proposed a self-assembled metal-polyphenol nanoparticle(TA-CU-CCM)loaded with natural products to reshape the local microenvironment and accelerate the healing process in diabetic wounds.The TA-CU-CCM nanoparticles were synthesized through the spontaneous coordination of tannic acid(TA)with copper ions(Cu^(2+)),followed by the incorporation of curcumin(CCM),which enhanced the aqueous dispersibility and stability of CCM.Our results demonstrated that this nanosystem significantly improved the biocompatibility and stability of CCM,ensured its sustainable release,and effectively inhibited the inflammatory response via the nuclear factor kappa-light-chain-enhancer of activated B cells(NF-κB)signaling pathway.Additionally,the nanoparticles exhibited notable antioxidant properties,efficiently scavenging ROS to alleviate oxidative stress in fibroblasts,while promoting endothelial cell migration and angiogenesis.In vivo studies further confirmed that the sprayable TA-CU-CCM remodeled the wound microenvironment by facilitating the transition from the pro-inflammatory M1 phenotype to the regenerative M2 phenotype,increasing superoxide dismutase(SOD)activity,and enhancing blood vessel density to promote wound healing in streptozotocin-induced diabetic mice.In conclusion,our findings demonstrate that this multifunctional drug-loaded metal polyphenol nanomedicine holds significant potential for enhancing diabetic wound healing.展开更多
Objective:To explore the wound healing potential and chemical components of Lindernia ruellioides from Mizoram,India.Methods:Plant extraction was conducted by cold maceration using chloroform,ethanol,and distilled wat...Objective:To explore the wound healing potential and chemical components of Lindernia ruellioides from Mizoram,India.Methods:Plant extraction was conducted by cold maceration using chloroform,ethanol,and distilled water as solvents.To guarantee the safety of the plant extract,an acute dermal toxicity test was conducted before the experiment.Antioxidant assays were performed.Excision and incision wound models were used to assess the wound healing activities,including wound closure rate,epithelialization period,and tensile strength.A histopathological examination was carried out.Results:Lindernia ruellioides contained bioactive compounds,such as mycolactone F,1-O-octadec-9-enyl glycerol,reserpine,tetracosanoic acid,2-O-caffeoylglucarate and several others which are found to possess various pharmacological activities.Acute dermal toxicity evaluation showed that the doses were deemed safe.Ethanol extract of Lindernia ruellioides(LREE)treatment significantly elevated glutathione,glutathione-S-transferase,and superoxide dismutase,and decreased malondialdehyde in a dose-dependent manner.It showed increased wound contraction rate,shorter epithelialization time,and elevated wound breaking strength in the LREE-treated group when compared with the control.In addition,the histological examination showed enhanced neovascularization,fibroblasts,and collagen in LREE-treated animals.Conclusions:Lindenia ruellioides exhibits remarkable effects on wound healing.The study validates the traditional use of this plant in Mizoram region as a wound-healing agent.展开更多
文摘The use of artificial intelligence(AI)in wound care has become a crucial instrument in improving the diagnosis,treatment,and management of chronic wounds.This study examines the several functions of AI in wound care,emphasizing its ability to better wound evaluation,hasten healing,alleviate pain,and improve cost-effectiveness.AI-driven technology,including mobile applications and intelligent bandages,provides precise wound assessment,tissue analysis,and continuous monitoring,hence allowing for tailored treatment strategies and prompt intervention.Moreover,AI-driven algorithms forecast wound healing trajectories and find individuals susceptible to chronic wounds,allowing prompt preventative interventions.There are also practical issues with integrating it into clinical practice.Adding AI to current healthcare systems,like electronic medical records(EMRs),needs careful planning and teamwork to make sure that AI-driven insights are used well in real-time clinical decision-making.To make sure AI is used safely,legal frameworks need to be set up to provide clear rules for its use in healthcare.These frameworks should include openness,risk management,and data evaluation.Lastly,clinical validation and acceptance are still big problems.Many AI-powered tools don't have enough clinical validation and aren't widely available,which makes it hard to use them in everyday clinical practice.Solving these problems is important for getting the most out of AI in wound care and making sure it is used safely and effectively.The article combines current advancements and prospective trajectories in AI-assisted wound care,highlighting its revolutionary ability to revolutionize patient outcomes and healthcare delivery.
基金supported by grants from the German Research Foundation (DFG)
文摘Chronic,non-healing wounds represent a significant challenge for healthcare systems worldwide,often requiring significant human and financial resources.Chronic wounds arise from the complex interplay of underlying comorbidities,such as diabetes or vascular diseases,lifestyle factors,and genetic risk profiles which may predispose extremities to local ischemia.Injuries are further exacerbated by bacterial colonization and the formation of biofilms.Infection,consequently,perpetuates a chronic inflammatory microenvironment,preventing the progression and completion of normal wound healing.The current standard of care(SOC)for chronic wounds involves surgical debridement along with localized wound irrigation,which requires inpatient care under general anesthesia.This could be followed by,if necessary,defect coverage via a reconstructive ladder utilizing wound debridement along with skin graft,local,or free flap techniques once the wound conditions are stabilized and adequate blood supply is restored.To promote physiological wound healing,a variety of approaches have been subjected to translational research.Beyond conventional wound healing drugs and devices that currently supplement treatments,cellular and immunotherapies have emerged as promising therapeutics that can behave as tailored therapies with cell-or molecule-specific wound healing properties.However,in contrast to the clinical omnipresence of chronic wound healing disorders,there remains a shortage of studies condensing the current body of evidence on cellular therapies and immunotherapies for chronic wounds.This review provides a comprehensive exploration of current therapies,experimental approaches,and translational studies,offering insights into their efficacy and limitations.Ultimately,we hope this line of research may serve as an evidence-based foundation to guide further experimental and translational approaches and optimize patient care long-term.
基金supports received from Scientific Research Fund of Liaoning Province Education Department(Grant No.JYTQN 2023025)Scientific Research Fund of Liaoning Province Education Department(Grant No.JYTQN 2023025)+3 种基金the Natural Science Foundation of Liaoning Province(Grant No.2024-MS-075)the National Natural Science Foundation of China(32201179)National Key R&D Program of China(2023YFC2508200)Liaoning Provincial Natural Science Foundation Joint Fund(General Support Program Project)(2023-MSBA-093).
文摘Refractory wounds cause significant harm to the health of patients and the most common treatments in clinical practice are surgical debridement and wound dressings.However,certain challenges,including surgical difficulty,lengthy recovery times,and a high recurrence rate persist.Conductive hydrogel dressings with combined monitoring and therapeutic properties have strong advantages in promoting wound healing due to the stimulation of endogenous current on wounds and are the focus of recent advancements.Therefore,this review introduces the mechanism of conductive hydrogel used for wound monitoring and healing,the materials selection of conductive hydrogel dressings used for wound monitoring,focuses on the conductive hydrogel sensor to monitor the output categories of wound status signals,proving invaluable for non-invasive,real-time evaluation of wound condition to encourage wound healing.Notably,the research of artificial intelligence(AI)model based on sensor derived data to predict the wound healing state,AI makes use of this abundant data set to forecast and optimize the trajectory of tissue regeneration and assess the stage of wound healing.Finally,refractory wounds including pressure ulcers,diabetes ulcers and articular wounds,and the corresponding wound monitoring and healing process are discussed in detail.This manuscript supports the growth of clinically linked disciplines and offers motivation to researchers working in the multidisciplinary field of conductive hydrogel dressings.
基金Supported by the National Natural Science Foundation of China,No.82202454 and No.81873934the Wang Zhengguo Trauma Medicine Fund(Growth Factor Revival Plan)No.SZYZ-TR-09+1 种基金the Shandong Provincial Natural Science Foundation Youth Fund,No.ZR2020QH168the Jinan Science and Technology Plan Project,No.202225065。
文摘BACKGROUND Diabetic foot ulcers(DFUs)are a significant challenge in diabetic care,and the efficacy of negative pressure wound therapy(NPWT)in treating them remains a subject of continuous investigation.AIM To provide a comprehensive meta-analysis of the role of NPWT in the manage-ment of DFUs.METHODS A systematic review was performed based on Preferred Reporting Items for Sys-tematic Reviews and Meta-Analyses guidelines,searching databases like PubMed,Embase,Web of Science,and the Cochrane Library.Randomized clinical trials(RCTs)were included to compare NPWT to other dressings for DFUs.Outcomes measured were wound healing time and rate,granulation tissue formation time,amputation rate,and adverse events.Study quality was evaluated using Coch-rane's risk of bias tool.Analyses utilizedχ2,I2,fixed or random-effects models via Stata v17.RESULTS Of the 1101 identified articles,9 RCTs were selected for meta-analysis.Studies spanned from 2005 to 2020 and originated from countries including the United States,Chile,Pakistan,Italy,India,and Germany.Meta-analysis demonstrated a significant improvement in wound healing rate[risk ratio(RR)=1.46,95%CI:1.22-1.76,P<0.01]and a reduction in amputation rate(RR=0.69,95%CI:0.50-0.96,P=0.006)with NPWT.Furthermore,the time for granulation tissue formation was significantly reduced by an average of 19.54 days.However,the incidence of adverse events did not significantly differ between NPWT and control treatments.CONCLUSION NPWT significantly improves wound healing rates and reduces amputation rates in DFUs.It also hastens the formation of granulation tissue.However,the therapy does not significantly alter the risk of adverse events compared to alternate treatments.
基金supported by Natural Science Foundation of Jilin Province(No.SKL202302002)。
文摘The healing of diabetic wounds poses a significant healthcare burden due to persistent inflammation,M1 macrophage aggregation,and high glucose levels in the microenvironment.Previous studies have demonstrated that immunomodulatory hydrogel dressings can facilitate diabetic wound healing.However,current immunomodulatory hydrogels require costly and complex treatments such as cell therapy and cytokines.Herein,a hierarchical hydrogel dressing with continuous biochemical gradient based on glycyrrhizic acid(GA) was constructed to modulate immunomodulatory processes in diabetic wounds.The hydrogels present many desirable features,such as tunable mechanical properties,broad antibacterial ability,outstanding conductive,transparent,and self-adhesive properties.The resultant hydrogel can promote diabetic wound healing by preventing bacterial infection,promoting macrophage polarization,improving the inflammatory microenvironment,and inducing angiogenesis and neurogenesis.Furthermore,electrical stimulation(ES) can further promote the healing of chronic diabetic wounds,providing valuable guidance for relevant clinical practice.
基金Supported by Henan Province Key Research and Development Program,No.231111311000Henan Provincial Science and Technology Research Project,No.232102310411+2 种基金Henan Province Medical Science and Technology Key Project,No.LHGJ20220566 and No.LHGJ20240365Henan Province Medical Education Research Project,No.WJLX2023079Zhengzhou Medical and Health Technology Innovation Guidance Program,No.2024YLZDJH022.
文摘Necrotizing fasciitis(NF)is a rapidly progressing,life-threatening soft tissue infection,with upper limb NF posing a particularly serious threat to patient survival and quality of life.Negative pressure wound therapy(NPWT)has shown considerable advantages in accelerating wound healing and mitigating functional impairment.A retrospective study by Lipatov et al.demonstrated that NPWT significantly reduced the time needed for wound closure preparation while enhancing the success rate of local repair.Despite its benefits,certain limitations highlight the need for further optimization.This paper investigates the potential for personalized dynamic regulation of NPWT,its integration with adjunctive therapies,and the role of multidisciplinary collaboration.Furthermore,it explores the incorporation of advanced technologies such as artificial intelligence,imaging modalities,and biomaterials,presenting novel pathways for the personalized management and global standardization of NF treatment.
基金financially supported by the National Natural Science Foun-dation of China(22278257)the Key R&D Program of Shaanxi Province(2024SF-YBXM-586)the Project of Innovation Capability Support Program in Shaanxi Province(2024ZC-KJXX-005)。
文摘Rapidly-advancing microneedle-based bioelectronics integrated with electrical stimulation(ES)therapy exhibit significant potential for improving chronic wound management.Herein,bio-inspired by the serrated structure of bee-stingers,we developed a temperature-sensitive,two-stage microneedle-based electro active platform(GP-PPy/PLA-MN)featuring rivet-like micros tructures that integrates intelligent,precise drug-releasing,ES-transmission,and real-time wound-assessment monitoring for comprehensive chronic wound-management and diagnostic therapy.The bionic-design mechanically anchors the microneedle beneath the skin's dermis,while GP-PPy/PLA-MN demonstrates versatile therapeutic characteristics,including outstanding biocompatibility,antimicrobial properties,and antimigratory origins.The GP-PPy/PLA-MN enables the sustained release of insulin at body temperature for up to24 hours through the poly-N-isopropyl acrylamide grafted amidated-gelatin-based thermo-sensitive hydrogel at the needle-tip,thereby providing long-term stable blood glucose control.GP-PPy/PLA-MN indicates its potential as a novel bioelectronics-based patch to record the temperature and humidity during the wound-healing process,realizing significant wound diagnosis and real-time wound assessment,and fundamentally facilitating the therapeutic efficacy by supplying solid data to protect the clinical practice.Extensive in vitro and in vivo studies have demonstrated that GP-PPy/PLA-MN can provide effective ES and sustained drug release,thereby promoting chronic wound healing and increasing the wound healing rate by 20%compared to the control group after 14 days of treatment.This innovative approach combines bioelectronics with intelligent drug delivery and microneedling technology to effectively address the critical challenges of chronic wound management,offering promising prospects for precision diagnostics and therapeutic interventions.
基金National Natural Science Foundation of China,No.81873934and Jinan Science and Technology Planning Project,No.202225065.
文摘BACKGROUND Mesenchymal stem cells,found in various tissues,possess significant healing and immunomodulatory properties,influencing macrophage polarization,which is essential for wound repair.However,chronic wounds present significant therapeutic challenges,requiring novel strategies to improve healing outcomes.AIM To investigate the potential of fetal dermal mesenchymal stem cells(FDMSCs)in enhancing wound healing through modulation of macrophage polarization,specifically by promoting the M2 phenotype to address inflammatory responses in chronic wounds.METHODS FDMSCs were isolated from BalB/C mice and co-cultured with RAW264.7 macrophages to assess their effects on macrophage polarization.Flow cytometry,quantitative reverse transcriptase polymerase chain reaction,and histological analyses were employed to evaluate shifts in macrophage phenotype and wound healing in a mouse model.Statistical analysis was performed using GraphPad Prism.RESULTS FDMSCs induced macrophage polarization from the M1 to M2 phenotype,as demonstrated by a reduction in proinflammatory markers(inducible nitric oxide synthase,interleukin-6)and an increase in anti-inflammatory markers[mannose receptor(CD206),arginase-1]in co-cultured RAW264.7 macrophages.These shifts were confirmed by flow cytometry.In an acute skin wound model,FDMSC-treated mice exhibited faster wound healing,enhanced collagen deposition,and improved vascular regeneration compared to controls.Significantly higher expression of arginase-1 further indicated an enriched M2 macrophage environment.CONCLUSION FDMSCs effectively modulate macrophage polarization from M1 to M2,reduce inflammation,and enhance tissue repair,demonstrating their potential as an immunomodulatory strategy in wound healing.These findings highlight the promising therapeutic application of FDMSCs in managing chronic wounds.
基金Hubei Province Top Medical Youth Talent Program,Wuhan Knowledge Innovation Special Basic Research Project,No.2023020201020558Clinical Research Project of Affiliated Hospital of Guangdong Medical University,No.LCYJ2021B004 and No.LCYJ2019B010Science and Technology Plan Project of Zhanjiang,No.2022A01191.
文摘BACKGROUND Diabetic foot ulcers(DFUs)present a significant clinical challenge due to their high prevalence and profound impact on morbidity.Ultrasound-assisted wound debridement(UAWD)has emerged as a potential therapeutic modality to improve healing outcomes in DFU management.AIM To evaluate the efficacy of UAWD in treating DFUs on wound closure rates,treatment duration,and quality of life outcomes.METHODS This systematic review and meta-analysis followed PRISMA guidelines,systematically searching PubMed,Embase,Web of Science,and the Cochrane Library with no date restrictions.Randomized controlled trials(RCTs)that evaluated the efficacy of UAWD in DFU treatment were included.Data were independently extracted by two reviewers,with discrepancies resolved through consensus or third-party consultation.The risk of bias was assessed using the Cochrane tool.χ2 and I2 statistics assessed heterogeneity,informing the use of fixed or random-effects models for meta-analysis,supplemented by sensitivity analysis and publication bias assessment through funnel plots and Egger's test.RESULTS From 1255 articles,seven RCTs met the inclusion criteria.The studies demonstrated that UAWD significantly reduced DFU healing time(standardized mean difference=-0.78,95%CI:-0.97 to-0.60,P<0.001)and increased healing rates(odds ratio=9.96,95%CI:5.99 to 16.56,P<0.001)compared to standard care.Sensitivity analysis confirmed the stability of these results,and no significant publication bias was detected.CONCLUSION UAWD is a promising adjunctive treatment for DFUs,significantly reducing healing times and increasing healing rates.These findings advocate for the integration of UAWD into standard DFU care protocols.
基金supported in part by the National Nature Science Foundation of China(92268206,81830064)the CAMS Innovation Fund for Medical Sciences(CIFMS,2019-I2M-5-059)+4 种基金the Military Medical Research Projects(145AKJ260015000X,2022-JCJQ-ZB-09600)the Military Key Basic Research of Foundational Strengthening Program(2020-JCJQ-ZD-256-021)the Science Foundation of National Defense Science and Technology for Excellent Young(2022-JCJQ-ZQ-017)the Military Medical Research and Development Projects(AWS17J005,2019-126)the Specific Research Fund of The Innovation Platform for Academicians of Hainan Province(YSPTZX202317).
文摘Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages,ultimately impairing its normal physiological function.Accumulating evidence underscores the potential of targeted modulation of mechanical cues to enhance skin regeneration,promoting scarless repair by influencing the extracellular microenvironment and driving the phenotypic transitions.The field of skin repair and skin appendage regeneration has witnessed remarkable advancements in the utilization of biomaterials with distinct physical properties.However,a comprehensive understanding of the underlying mechanisms remains somewhat elusive,limiting the broader application of these innovations.In this review,we present two promising biomaterial-based mechanical approaches aimed at bolstering the regenerative capacity of compromised skin.The first approach involves leveraging biomaterials with specific biophysical properties to create an optimal scarless environment that supports cellular activities essential for regeneration.The second approach centers on harnessing mechanical forces exerted by biomaterials to enhance cellular plasticity,facilitating efficient cellular reprogramming and,consequently,promoting the regeneration of skin appendages.In summary,the manipulation of mechanical cues using biomaterial-based strategies holds significant promise as a supplementary approach for achieving scarless wound healing,coupled with the restoration of multiple skin appendage functions.
基金the Talent Management Project of Prince of Songkla University
文摘Wearable sensing systems have been designed to monitor health conditions in real-time by detecting analytes in human biofluids.Wound diagnosis remains challenging,necessitating suitable materials for high-performance wearable sensors to offer prompt feedback.Existing devices have limitations in measuring pH and the concentration of pH-dependent electroactive species simultaneously,which is crucial for obtaining a comprehensive understanding of wound status and optimizing biosensors.Therefore,improving materials and analysis system accuracy is essential.This article introduces the first example of a flexible array capable of detecting pyocyanin,a bacterial virulence factor,while correcting dynamic pH fluctuations.We demonstrate that this combined sensor enhances accuracy by mitigating the impact of pH variability on pyocyanin sensor response.Customized screen-printable inks were developed to enhance analytical performance.The analytical performances of two sensitive sensor systems(i.e.,fully-printed porous graphene/multiwalled carbon nanotube(CNT)and polyaniline/CNT composites for pyocyanin and pH sensors)are evaluated.Partial least square regression is employed to analyze nonzero-order data arrays from square wave voltammetric and potentiometric measurements of pyocyanin and pH sensors to establish a predictive model for pyocyanin concentration in complex fluids.This sensitive and effective strategy shows potential for personalized applications due to its affordability,ease of use,and ability to adjust for dynamic pH changes.
基金supported by the National Natural Science Foundation of China(No.82272297).
文摘Abnormal wound scarring often leads to functional impairments and cosmetic deformities,primarily driven by the prolonged activation of the TGF-β/Smad signaling pathway.Addressing this challenge,we developed a biomimetic scaffold aimed at facilitating rapid and scarless wound healing.This highly in-tegrated 3D-printed dermal scaffold comprised modified recombinant human type III collagen(rhCOLIII-MA),gelatin methacrylate(GelMA),and liposomes encapsulating SB431542 to target TGF-β1(Lip@SB).The rhCOLIII-MA/GelMA(CG)scaffold retained inherent biomaterial characteristics,exhibited tailored physicochemical properties,and demonstrated favorable biocompatibility.Moreover,the Lip@SB-loaded CG scaffold(CGL)effectively promoted in vitro wound healing,while enabling controlled release of SB431542 to inhibit pathological collagen deposition.In a full-thickness skin defect rat model,the CGL dermal scaffold combined with split-thickness skin graft(STSG)minimized scar contraction,stimulated functional neovascularization,and enhanced graft aesthetics comparable to normal skin.Remarkably,the performance of the CGL scaffold surpassed that of commercially available anti-scarring alternatives.This innovative strategy presents a straightforward approach toward scarless skin regeneration and holds promise in alleviating the prolonged,painful postoperative rehabilitation.
基金supported by the Fundamental Research Funds for the Central Universities(No.20720230037)the National Natural Science Foundation of China(No.52273305)+2 种基金Natural Science Foundation of Fujian Province of China(No.2023J05012)State Key Laboratory of Vaccines for Infectious Diseases,Xiang An Biomedicine Laboratory(Nos.2023XAKJ0103071,2023XAKJ0102061)Natural Science Foundation of Xiamen,China(No.3502Z20227010).
文摘Histopathological analysis of chronic wounds is crucial for clinicians to accurately assess wound healing progress and detect potential malignancy.However,traditional pathological tissue sections require specific staining procedures involving carcinogenic chemicals.This study proposes an interdisciplinary approach merging materials science,medicine,and artificial intelligence(AI)to develop a virtual staining technique and intelligent evaluation model based on deep learning for chronic wound tissue pathology.This innovation aims to enhance clinical diagnosis and treatment by offering personalized AI-driven therapeutic strategies.By establishing a mouse model of chronic wounds and using a series of hydrogel wound dressings,tissue pathology sections were periodically collected for manual staining and healing assessment.We focused on leveraging the pix2pix image translation framework within deep learning networks.Through CNN models implemented in Python using PyTorch,our study involves learning and feature extraction for region segmentation of pathological slides.Comparative analysis between virtual staining and manual staining results,along with healing diagnosis conclusions,aims to optimize AI models.Ultimately,this approach integrates new metrics such as image recognition,quantitative analysis,and digital diagnostics to formulate an intelligent wound assessment model,facilitating smart monitoring and personalized treatment of wounds.In blind evaluation by pathologists,minimal disparities were found between virtual and conventional histologically stained images of murine wound tissue.The evaluation used pathologists’average scores on real stained images as a benchmark.The scores for virtual stained images were 71.1%for cellular features,75.4%for tissue structures,and 77.8%for overall assessment.Metrics such as PSNR(20.265)and SSIM(0.634)demonstrated our algorithms’superior performance over existing networks.Eight pathological features such as epidermis,hair follicles,and granulation tissue can be accurately identified,and the images were found to be more faithful to the actual tissue feature distribution when compared to manually annotated data.
基金supported by European Regional Development Funds RE0022527 ZEBRATOX(EU-Région Réunion-French State national counterpart,to Nicolas Diotel and Jean-Loup Bascands).
文摘After brain damage,regenerative angiogenesis and neurogenesis have been shown to occur simultaneously in mammals,suggesting a close link between these processes.However,the mechanisms by which these processes interact are not well understood.In this work,we aimed to study the correlation between angiogenesis and neurogenesis after a telencephalic stab wound injury.To this end,we used zebrafish as a relevant model of neuroplasticity and brain repair mechanisms.First,using the Tg(fli1:EGFP×mpeg1.1:mCherry)zebrafish line,which enables visualization of blood vessels and microglia respectively,we analyzed regenerative angiogenesis from 1 to 21 days post-lesion.In parallel,we monitored brain cell proliferation in neurogenic niches localized in the ventricular zone by using immunohistochemistry.We found that after brain damage,the blood vessel area and width as well as expression of the fli1 transgene and vascular endothelial growth factor(vegfaa and vegfbb)were increased.At the same time,neural stem cell proliferation was also increased,peaking between 3 and 5 days post-lesion in a manner similar to angiogenesis,along with the recruitment of microglia.Then,through pharmacological manipulation by injecting an anti-angiogenic drug(Tivozanib)or Vegf at the lesion site,we demonstrated that blocking or activating Vegf signaling modulated both angiogenic and neurogenic processes,as well as microglial recruitment.Finally,we showed that inhibition of microglia by clodronate-containing liposome injection or dexamethasone treatment impairs regenerative neurogenesis,as previously described,as well as injury-induced angiogenesis.In conclusion,we have described regenerative angiogenesis in zebrafish for the first time and have highlighted the role of inflammation in this process.In addition,we have shown that both angiogenesis and neurogenesis are involved in brain repair and that microglia and inflammation-dependent mechanisms activated by Vegf signaling are important contributors to these processes.This study paves the way for a better understanding of the effect of Vegf on microglia and for studies aimed at promoting angiogenesis to improve brain plasticity after brain injury.
基金financially supported by the National Natural Science Foundation of China(No.82030056)Postdoctoral Innovation Talents Support Program of China(No.BX20230489)。
文摘The treatment of skin wounds,especially chronic wounds,remains a critical clinical challenge and places a heavy burden on patients and healthcare systems.In recent years,the engineering strategy of using biomaterial-assisted exosomes has emerged as a powerful tool for skin repair.Compared to treatments such as debridement and regular dressing changes,the design of biomaterial-assisted exosomes not only maintains the bioactivity of exosomes at the wound site but also provides an appropriate microenvironment for the repair of complex tissues,thereby accelerating wound healing.This review systematically introduces the general characteristics of exosomes and their functions in skin wound healing,highlights recent advances in classification of natural exosomes and engineering methods which enriching their functions in intercellular communication.Then,various emerging and innovative approaches based on biomaterials delivery of exosomes are comprehensively discussed.The review seeks to bring an in-depth understanding of bioactive dressings based on exosomes therapeutic strategies,aiming to facilitate new clinical application value.
基金supported by Shanghai Special Project on Artificial Intelligence Facilitating the Reform of Scientific Research Paradigms to Empower Disciplinary Advancement(No.Z-2024-114-022)the Horizontal Project of Fuzhou Water Quality Monitoring Co.,Ltd.(No.H-2025-312-012)+2 种基金the General Program of the National Natural Science Foundation of China(No.82073266)Science and Technology Research Project of Jiangxi Provincial Department of Education(No.GJJ2201639)the Doctoral Research Start-up Fund Project of Jinggangshan University(No.JZB2117).
文摘Wound management remains significant challenges,encompassing numerous risks such as infection,antibiotic resistance,tissue adhesion,ischemia,hypoxia,and inflammatory responses.These complications are particularly acute in cases of bacterial infection,where conventional treatment modalities may fail to provide adequate efficacy.To address these challenges,this study introduces phytic acid(PA)@ε-polylysine(Ply)@curcumin(Cur)@Zn@Gauze(PPCZ@Gauze),an innovative multifunctional gauze tailored to enhance wound healing.PPCZ@Gauze is designed and prepared using biosafe materials(phytic acid,ε-polylysine,curcumin,and zinc ions)through advanced surface modification techniques.Both in vitro and in vivo experiments demonstrate remarkable antibacterial properties,reduced tissue adhesion,and accelerated wound healing capabilities of PPCZ@Gauze compared to conventional medical gauze.Notably,PPCZ@Gauze also exhibits ease of preparation,cost-effectiveness,and scalability for large-scale production.Overall,this innovative multifunctional gauze offers a promising solution for managing bacterial infection wounds,leading to enhanced therapeutic outcomes and patient care.
文摘The management of chronic wounds remains a substantial challenge for healthcare providers. Inadequate wound care can result in serious complications, including infection, which may ultimately lead to amputation or even death. While traditional excipients exhibit some efficacy in promoting wound healing, they are not sufficiently effective in preventing wound infections. As an antimicrobial metal, copper has a long history in the antimicrobial field, and at the same time, wound auxiliaries with copper ions have also been used in the treatment of chronic wounds. To address the limitations of conventional wound dressings, including insufficient antimicrobial properties and limited capacity to promote wound healing, this study introduces a highly adhesive hydrogel with superior mechanical stability for non-invasive wound treatment. The hydrogel was composed of carboxymethyl chitosan, tannic acid and copper ions. The tannic acid solution was subjected to dropwise addition of CuCl2 solution to produce precipitation, and tannic acid/copper ions (TA/Cu2+) composite nanoparticles were prepared. Through topological adhesion, the CMCS with pH sensitivity has the ability to establish adhesive connections with a wide range of materials. The benefits of CMCS/TA/Cu2+ hydrogel, as a kind of wound closure and repair material, include efficient wound closure, and resistance against bacterial invasion while maintaining cleanliness. Additionally, it exhibits excellent tensile and mechanical stability that can facilitate effective closure and repair in dynamic areas like joint wounds. This promising hydrogel adhesive has demonstrated potential as a material for wound closure and restoration.
基金supported by Key Scientific and Technological Project of Henan Province(No.242102231060)Doctoral Scientific Research Foundation of Zhoukou Normal University(No.ZKNUC2021041)the Program of Innovative Research Team(in Science and Technology)in University of Henan Province(No.23IRTSTHN008)。
文摘Bacterial infection,insufficient angiogenesis,and oxidative damage are generally regarded as key issues that impede wound healing,making it necessary to prepare new biomaterials to simultaneously address these problems.In this work,monodispersed CeO_(2)@CuS nanocomposites(NCs)were successfully prepared with tannin(TA)as the reductant and linker.Due to abundant oxygen vacancies in CeO_(2)and the polyphenolic structure of TA,the TA-CeO_(2)@CuS NCs exhibited a remarkable antioxidant ability to scavenge excessive reactive oxygen species(ROS),which would likely induce serious inflammation.In addition,the TA-CeO_(2)@CuS NCs demonstrated excellent antibacterial capability with near-infrared ray(NIR)irradiation,and the released copper ions could promote the regeneration of blood vessels.These synergistic effects indicated that the synthesized TA-CeO_(2)@CuS NCs could serve as a promising biomaterial for multimodal wound therapy.
基金supported by the Key Research&Development Program of Guangxi(No.GuiKeAB22080088)the National Natural Science Foundation of China(Nos.52173150,U22A20315 and 82360372)+1 种基金the National Traditional Chinese Medicine Advantageous Specialty Construction Project(Intensive Care Unit)Guangzhou Science and Technology Program City-University Joint Funding Project(Nos.2023A03J0001 and 2024A03J0604).
文摘Diabetes wounds present a complex microenvironment characterized by persistent inflammation,elevated reactive oxygen species(ROS),and compromised vascular conditions,all of which contribute to delayed or incomplete healing.Although advances in wound care have been made,effective strategies to address these multifaceted challenges remain limited.In this study,we proposed a self-assembled metal-polyphenol nanoparticle(TA-CU-CCM)loaded with natural products to reshape the local microenvironment and accelerate the healing process in diabetic wounds.The TA-CU-CCM nanoparticles were synthesized through the spontaneous coordination of tannic acid(TA)with copper ions(Cu^(2+)),followed by the incorporation of curcumin(CCM),which enhanced the aqueous dispersibility and stability of CCM.Our results demonstrated that this nanosystem significantly improved the biocompatibility and stability of CCM,ensured its sustainable release,and effectively inhibited the inflammatory response via the nuclear factor kappa-light-chain-enhancer of activated B cells(NF-κB)signaling pathway.Additionally,the nanoparticles exhibited notable antioxidant properties,efficiently scavenging ROS to alleviate oxidative stress in fibroblasts,while promoting endothelial cell migration and angiogenesis.In vivo studies further confirmed that the sprayable TA-CU-CCM remodeled the wound microenvironment by facilitating the transition from the pro-inflammatory M1 phenotype to the regenerative M2 phenotype,increasing superoxide dismutase(SOD)activity,and enhancing blood vessel density to promote wound healing in streptozotocin-induced diabetic mice.In conclusion,our findings demonstrate that this multifunctional drug-loaded metal polyphenol nanomedicine holds significant potential for enhancing diabetic wound healing.
文摘Objective:To explore the wound healing potential and chemical components of Lindernia ruellioides from Mizoram,India.Methods:Plant extraction was conducted by cold maceration using chloroform,ethanol,and distilled water as solvents.To guarantee the safety of the plant extract,an acute dermal toxicity test was conducted before the experiment.Antioxidant assays were performed.Excision and incision wound models were used to assess the wound healing activities,including wound closure rate,epithelialization period,and tensile strength.A histopathological examination was carried out.Results:Lindernia ruellioides contained bioactive compounds,such as mycolactone F,1-O-octadec-9-enyl glycerol,reserpine,tetracosanoic acid,2-O-caffeoylglucarate and several others which are found to possess various pharmacological activities.Acute dermal toxicity evaluation showed that the doses were deemed safe.Ethanol extract of Lindernia ruellioides(LREE)treatment significantly elevated glutathione,glutathione-S-transferase,and superoxide dismutase,and decreased malondialdehyde in a dose-dependent manner.It showed increased wound contraction rate,shorter epithelialization time,and elevated wound breaking strength in the LREE-treated group when compared with the control.In addition,the histological examination showed enhanced neovascularization,fibroblasts,and collagen in LREE-treated animals.Conclusions:Lindenia ruellioides exhibits remarkable effects on wound healing.The study validates the traditional use of this plant in Mizoram region as a wound-healing agent.
