Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production.In order to get more informations on defense related EST sequences and help breeding program,we constructed and ...Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production.In order to get more informations on defense related EST sequences and help breeding program,we constructed and characterized a suppression subtractive hybridization cDNA library with artificially inoculated leaves and control Chinese wild Vitis pseudoreticulata clone Baihe-35-1,which is highly resistant to powdery mildew.In the library,the length of 58 EST fragments known as putative functions varied from 130 to 800 bp,and 60%of the ESTs exhibited high similarity to known sequences in database of GenBank with BLASTX analysis.These genes were involved in stress/defense response,detoxification,signal transduction,disease defense,and etc.,and 14 ESTs remained unknown or hypothetical proteins,which may be new genes.The experiment provided an important basis for studying the disease-resistance mechanism and obtaining the genes for the aim of improving grapevine powdery mildew resistance.展开更多
基金funded by the National Natural Science Foundation of China(30571280,30771493)the Transcentury Talent-Training Program of Ministry of Education of China and the National 863 Project of China(2007AA10Z182)
文摘Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production.In order to get more informations on defense related EST sequences and help breeding program,we constructed and characterized a suppression subtractive hybridization cDNA library with artificially inoculated leaves and control Chinese wild Vitis pseudoreticulata clone Baihe-35-1,which is highly resistant to powdery mildew.In the library,the length of 58 EST fragments known as putative functions varied from 130 to 800 bp,and 60%of the ESTs exhibited high similarity to known sequences in database of GenBank with BLASTX analysis.These genes were involved in stress/defense response,detoxification,signal transduction,disease defense,and etc.,and 14 ESTs remained unknown or hypothetical proteins,which may be new genes.The experiment provided an important basis for studying the disease-resistance mechanism and obtaining the genes for the aim of improving grapevine powdery mildew resistance.
