The ability of wheat to adapt to a wide range of environmental conditions is determined mostly by allelic diversity among genes regulating vernalization requirement.Vrn-1 is a major regulator of this requirement.In th...The ability of wheat to adapt to a wide range of environmental conditions is determined mostly by allelic diversity among genes regulating vernalization requirement.Vrn-1 is a major regulator of this requirement.In this study,two novel alleles of Vrn-A1 were discovered in Chinese cultivars:vrn-A1n was identified in two landraces,Jiunong 2 and Ganchun 16,and Vrn-A1o was detected in Duanhongmangmai.Both novel alleles showed a linked duplication in the promoter region.The common copy of these two alleles was identical to the recessive allele vrn-A1.Compared with the recessive allele vrn-A1,the other copy of vrn-A1n contained a 54-bp deletion in the promoter region and the distinct copy of Vrn-A1o contained an11-bp deletion in the promoter region.In segregating populations in the greenhouse under nonvernalizing(20–25°C)and long-day(16 h light)conditions,plants with the novel vrn-A1n allele did not head earlier than those with the recessive vrn-A1 allele.However,plants that were either homozygous or heterozygous for the novel Vrn-A1o allele headed earlier than those with the recessive vrn-A1 allele.To identify the novel allele with the small-sized product and facilitate screening,a DNA marker for the novel dominant allele Vrn-A1o was designed.Analysis of the novel-allele distribution showed that two cultivars carrying the vrn-A1n allele were dispersed in the northwestern spring wheat zone,and 12 cultivars carrying the dominant Vrn-A1o allele were widely distributed in the northwestern spring wheat zone,Xinjiang winter and spring wheat zone,Yellow and Huai River valley winter wheat zone,and Qinghai-Tibetan Plateau spring and winter wheat zone.Our study identifies useful germplasm and a DNA marker for wheat breeding.展开更多
Dear Editor,Common wheat(Triticum aestivum L.)is an important food crop worldwide(Xiao et al.,2022).Understanding how heading date and plant architecture are synergistically regulated in wheat is critical for breeding...Dear Editor,Common wheat(Triticum aestivum L.)is an important food crop worldwide(Xiao et al.,2022).Understanding how heading date and plant architecture are synergistically regulated in wheat is critical for breeding elite varieties(Koppolu et al.,2022).The vernalization gene VERNALIZATION1(VRN1)was cloned more than two decades ago in winter wheat and encodes a MADS-box transcription factor that affects flowering time in response to vernalization(Yan et al.,2003;Xu and Chong,2018).VRN1 and its closest homologs are also involved in the regulation of plant height in the tetraploid wheat cultivar Kronos(Chen and Dubcovsky,2012;Li et al.,2019).However,the molecular mechanisms by which VRN1 regulates heading date and plant height in wheat remain unclear.展开更多
基金funded by the National Key Research and Development Program of China(2016YFD0101802)the Key Research and Development Project of Shaanxi Province(2019ZDLNY04-05)+1 种基金the National Basic Research Program of China(2014CB138102)the National Natural Science Foundation of China(30971770 and 31671693)。
文摘The ability of wheat to adapt to a wide range of environmental conditions is determined mostly by allelic diversity among genes regulating vernalization requirement.Vrn-1 is a major regulator of this requirement.In this study,two novel alleles of Vrn-A1 were discovered in Chinese cultivars:vrn-A1n was identified in two landraces,Jiunong 2 and Ganchun 16,and Vrn-A1o was detected in Duanhongmangmai.Both novel alleles showed a linked duplication in the promoter region.The common copy of these two alleles was identical to the recessive allele vrn-A1.Compared with the recessive allele vrn-A1,the other copy of vrn-A1n contained a 54-bp deletion in the promoter region and the distinct copy of Vrn-A1o contained an11-bp deletion in the promoter region.In segregating populations in the greenhouse under nonvernalizing(20–25°C)and long-day(16 h light)conditions,plants with the novel vrn-A1n allele did not head earlier than those with the recessive vrn-A1 allele.However,plants that were either homozygous or heterozygous for the novel Vrn-A1o allele headed earlier than those with the recessive vrn-A1 allele.To identify the novel allele with the small-sized product and facilitate screening,a DNA marker for the novel dominant allele Vrn-A1o was designed.Analysis of the novel-allele distribution showed that two cultivars carrying the vrn-A1n allele were dispersed in the northwestern spring wheat zone,and 12 cultivars carrying the dominant Vrn-A1o allele were widely distributed in the northwestern spring wheat zone,Xinjiang winter and spring wheat zone,Yellow and Huai River valley winter wheat zone,and Qinghai-Tibetan Plateau spring and winter wheat zone.Our study identifies useful germplasm and a DNA marker for wheat breeding.
基金supported by the National Key Research and Development Program of China(2022YFF1002902)the Biological Breeding-National Science and Technology Major Project(2023ZD0406802)+1 种基金the Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-CSCB-202401,CAAS-ZDRW202403)the Taishan Scholars Program(tstp20240843).
文摘Dear Editor,Common wheat(Triticum aestivum L.)is an important food crop worldwide(Xiao et al.,2022).Understanding how heading date and plant architecture are synergistically regulated in wheat is critical for breeding elite varieties(Koppolu et al.,2022).The vernalization gene VERNALIZATION1(VRN1)was cloned more than two decades ago in winter wheat and encodes a MADS-box transcription factor that affects flowering time in response to vernalization(Yan et al.,2003;Xu and Chong,2018).VRN1 and its closest homologs are also involved in the regulation of plant height in the tetraploid wheat cultivar Kronos(Chen and Dubcovsky,2012;Li et al.,2019).However,the molecular mechanisms by which VRN1 regulates heading date and plant height in wheat remain unclear.
