Endophytic fungi associated with medicinal plants are reservoirs of compounds with therapeutic properties.The present study focused on the isolation and identification of endophytic fungi from an ethnomedicinal orchid...Endophytic fungi associated with medicinal plants are reservoirs of compounds with therapeutic properties.The present study focused on the isolation and identification of endophytic fungi from an ethnomedicinal orchid,Vanda cristata Wall.ex Lindl.,and the assessment of their bioactive potential through screening their phytochemical content,antioxidant property,antimicrobial activity,and cytotoxicity,followed by metabolite profiling of the promising isolate extract.Colletotrichum taiwanense BPSRJ3 extract showed the highest total phenolic and flavonoid content.Although all isolate extracts showed broad spectrum antimicrobial activity,C.taiwanense BPSRJ3 extract showed the widest zone of inhibition and lowest MIC against the test pathogens.It also exhibited cytotoxicity against MCF-7 and A549 human cancer cell lines while having no discernible cytotoxic impact on the HEK-293 human normal cell line.Metabolites of C.taiwanense BPSRJ3 extract were characterized using FT-IR spectroscopy and GC-MS analysis,providing insights into the biologically active compounds con-tributing to its bioactivity.It is the first study to report C.taiwanense isolated from V.cristata as having bioactive potential.The findings of this study provide opportunities for more investigation into using the advantages of this fungal endophyte in the pharmaceutical sector.展开更多
Advancement in field of GIS and Information Technology has taken conservation works and strategies a step further as most conservation works are now dependent on these technologies. The present study explores the pred...Advancement in field of GIS and Information Technology has taken conservation works and strategies a step further as most conservation works are now dependent on these technologies. The present study explores the prediction ability of MAXENT using a very low sample size by applying jackknife analysis over a well defined smaller region and using only climate data. Vanda bicolor is a horticulture important orchid grown in certain patches of North Eastern region of India and the species considered to be “Vulnerable”. Present study reports a distribution prediction model using different geo-climatic parameters for a small area. Model validation by ground truthing gives a significant successful result which clearly defines the ability of MAXENT prediction model to give high success rate (71%) with low training samples. Use of the low sample size over a larger area results in unstable models however application of these samples in smaller radius around the occurrence points could provide good working models.展开更多
We used standard isolation protocols to explore the endophytic fungal communities in three tissue types of two dominant orchids （Bulbophyllum neilgherrense and Vanda testacea） of the Kaiga forest of the Western Ghat...We used standard isolation protocols to explore the endophytic fungal communities in three tissue types of two dominant orchids （Bulbophyllum neilgherrense and Vanda testacea） of the Kaiga forest of the Western Ghats. We surface sterilized and assessed 90 segments of each orchid for the occurrence and diversity of endophytic fungal taxa. The 118 fungal isolates were obtained from root, bulb and leaves of B. neilgherrense, consisting of 17 anamorphic taxa （range, 10-15 taxa） with 1.3 fungal taxa per segment （range, 1.2-1.4 taxa）. Four taxa （Aspergillus flavus, A. niger, Penicillium sp. and morpho sp. 1） belonged to the core group （11.1%--32.2%）. The relative abundance of A. flavus and morpho sp. 1 was more than 10%. A total of 130 fungal isolates from roots, stems and leaves of V. testacea yielded 20 anamorphic taxa （range, 11-15 taxa） with 1.4 fungal taxa per segment （range, 1.4-1.5 taxa）. Aspergillusflavus, A. niger, A. ochraceus, Gliocladium viride, Penicillium sp. and morpho sp. 1 belonged to the core group. Relative abundance exceeded 10% for A. flavus, A. niger, and morpho sp. 1. The Simpson and Shannon diversity indices were higher in leaf than root or bulb/stem of both orchids. Jaccard＇s similarity coefficient was higher between root and leaf in both orchids （56.3%-60%） than between other pairs. Our study revealed that the endophytic fungal assemblage and diversity orB. neilgherrense and E testacea of Kaiga forest of the Western Ghats were relatively similar between orchids and their tissues.展开更多
基金Bishal Pun acknowledges the financial assistance received in the form of a fellowship under DBT-JRF Program(DBT/2020/NEHU/1333)from Department of Biotechnology(DBT),Government of India.
文摘Endophytic fungi associated with medicinal plants are reservoirs of compounds with therapeutic properties.The present study focused on the isolation and identification of endophytic fungi from an ethnomedicinal orchid,Vanda cristata Wall.ex Lindl.,and the assessment of their bioactive potential through screening their phytochemical content,antioxidant property,antimicrobial activity,and cytotoxicity,followed by metabolite profiling of the promising isolate extract.Colletotrichum taiwanense BPSRJ3 extract showed the highest total phenolic and flavonoid content.Although all isolate extracts showed broad spectrum antimicrobial activity,C.taiwanense BPSRJ3 extract showed the widest zone of inhibition and lowest MIC against the test pathogens.It also exhibited cytotoxicity against MCF-7 and A549 human cancer cell lines while having no discernible cytotoxic impact on the HEK-293 human normal cell line.Metabolites of C.taiwanense BPSRJ3 extract were characterized using FT-IR spectroscopy and GC-MS analysis,providing insights into the biologically active compounds con-tributing to its bioactivity.It is the first study to report C.taiwanense isolated from V.cristata as having bioactive potential.The findings of this study provide opportunities for more investigation into using the advantages of this fungal endophyte in the pharmaceutical sector.
文摘Advancement in field of GIS and Information Technology has taken conservation works and strategies a step further as most conservation works are now dependent on these technologies. The present study explores the prediction ability of MAXENT using a very low sample size by applying jackknife analysis over a well defined smaller region and using only climate data. Vanda bicolor is a horticulture important orchid grown in certain patches of North Eastern region of India and the species considered to be “Vulnerable”. Present study reports a distribution prediction model using different geo-climatic parameters for a small area. Model validation by ground truthing gives a significant successful result which clearly defines the ability of MAXENT prediction model to give high success rate (71%) with low training samples. Use of the low sample size over a larger area results in unstable models however application of these samples in smaller radius around the occurrence points could provide good working models.
文摘We used standard isolation protocols to explore the endophytic fungal communities in three tissue types of two dominant orchids （Bulbophyllum neilgherrense and Vanda testacea） of the Kaiga forest of the Western Ghats. We surface sterilized and assessed 90 segments of each orchid for the occurrence and diversity of endophytic fungal taxa. The 118 fungal isolates were obtained from root, bulb and leaves of B. neilgherrense, consisting of 17 anamorphic taxa （range, 10-15 taxa） with 1.3 fungal taxa per segment （range, 1.2-1.4 taxa）. Four taxa （Aspergillus flavus, A. niger, Penicillium sp. and morpho sp. 1） belonged to the core group （11.1%--32.2%）. The relative abundance of A. flavus and morpho sp. 1 was more than 10%. A total of 130 fungal isolates from roots, stems and leaves of V. testacea yielded 20 anamorphic taxa （range, 11-15 taxa） with 1.4 fungal taxa per segment （range, 1.4-1.5 taxa）. Aspergillusflavus, A. niger, A. ochraceus, Gliocladium viride, Penicillium sp. and morpho sp. 1 belonged to the core group. Relative abundance exceeded 10% for A. flavus, A. niger, and morpho sp. 1. The Simpson and Shannon diversity indices were higher in leaf than root or bulb/stem of both orchids. Jaccard＇s similarity coefficient was higher between root and leaf in both orchids （56.3%-60%） than between other pairs. Our study revealed that the endophytic fungal assemblage and diversity orB. neilgherrense and E testacea of Kaiga forest of the Western Ghats were relatively similar between orchids and their tissues.
