目的从分子水平研究中等浓度气态甲醛环境暴露对呼吸系统中类香草素受体(van illo id recep-tor sub type 1,VR 1)基因表达的影响。方法用WH-2型环境气候舱释放的甲醛和净空气,分别通入2只各放有5只幼年大鼠的染毒缸中连续灌流72 h后,...目的从分子水平研究中等浓度气态甲醛环境暴露对呼吸系统中类香草素受体(van illo id recep-tor sub type 1,VR 1)基因表达的影响。方法用WH-2型环境气候舱释放的甲醛和净空气,分别通入2只各放有5只幼年大鼠的染毒缸中连续灌流72 h后,迅速取肺,用RT-PCR方法检测大鼠在中等浓度甲醛环境暴露下肺VR 1和管家基因G 3PD表达水平。结果中等浓度甲醛3.0 m g/m3灌流比清洁空气灌流导致幼年大鼠肺VR 1-mRNA表达增加。结论中等浓度甲醛灌流导致幼年大鼠肺部VR 1-mRNA表达量增加。展开更多
Improved yield potential is the goal of barley domestication and cultivation.During this process,two-and six-rowed barley types emerged and have been utilised in breeding and production.The six-rowed type could produc...Improved yield potential is the goal of barley domestication and cultivation.During this process,two-and six-rowed barley types emerged and have been utilised in breeding and production.The six-rowed type could produce three times as many grains as its ancestral two-rowed forms,thus dominating barley cultivation for thousands of years.The deficiens form of the two-rowed type,characterised by extremely suppressed lateral spikelets,has gained dominance over the past few decades in barley-growing regions worldwide.We hypothesised that the absence of lateral spikelets in deficiens barley affects spike architecture and spike-related traits,contributing to its superior yield potential of deficiens barley cultivation.Currently,a deficiens barley variety,RGT Planet,is the most popular barley variety in the world.In this study,we used two F_(2) populations derived from crossing RGT Planet with two canonical two-rowed barley and identified the functional allele Vrs1.t1 associated with deficiens morphology.We observed that the Vrs1.t1 allele may contribute to high yield potential by optimising spike architecture through increased spikelet length,grain number,and grain size.Phylogenetic analysis suggests that the deficiens mutation was likely present from the early stages of barley cultivation in the Fertile Crescent and spread to Ethiopia and beyond with agricultural expansion.We conclude that the ancient deficiens allele Vrs1.t1 has been a critical driver for the recent success of modern barley improvement by optimising spike architecture.展开更多
最近,有两种接受伤害性热刺激的受体被克隆,分别命名为VR1(Vanilloid receptor subtype 1)和VRL1(Vanilloid receptor like protein 1)。本研究探讨了VR1作为原发性感觉神经元和伤害感受神经元可认识的肽P物质(SP)与降钙素基因关联肽(CG...最近,有两种接受伤害性热刺激的受体被克隆,分别命名为VR1(Vanilloid receptor subtype 1)和VRL1(Vanilloid receptor like protein 1)。本研究探讨了VR1作为原发性感觉神经元和伤害感受神经元可认识的肽P物质(SP)与降钙素基因关联肽(CGRP)能神经元的关系。展开更多
目的:探索非致炎浓度的福尔马林对小鼠皮肤辣椒素受体(vanilloid receptor type 1,VR1)的激活作用。方法:选用KM小鼠随机分为3组:福尔马林(formalin,FM)组、辣椒素(capsaicin,CAP)组和Capsazepine(CPZ)组。采用注射后5 m in内小鼠舔舐...目的:探索非致炎浓度的福尔马林对小鼠皮肤辣椒素受体(vanilloid receptor type 1,VR1)的激活作用。方法:选用KM小鼠随机分为3组:福尔马林(formalin,FM)组、辣椒素(capsaicin,CAP)组和Capsazepine(CPZ)组。采用注射后5 m in内小鼠舔舐注射脚掌的时间作为评价疼痛反应的指标,对各组小鼠的痛反应进行观测。结果:0.025%FM和200μmol/L CAP是理想的实验浓度,既能引起显著疼痛反应,又不引起炎性损伤的致痛浓度,适于研究受体拮抗剂的抗疼痛作用;1μmol/L CPZ分别与200μmol/L CAP和0.025%FM共注射能有效的拮抗CAP和FM引起的疼痛反应。结论:非致炎浓度FM所致的疼痛反应在外周可能由VR1介导。展开更多
文摘目的从分子水平研究中等浓度气态甲醛环境暴露对呼吸系统中类香草素受体(van illo id recep-tor sub type 1,VR 1)基因表达的影响。方法用WH-2型环境气候舱释放的甲醛和净空气,分别通入2只各放有5只幼年大鼠的染毒缸中连续灌流72 h后,迅速取肺,用RT-PCR方法检测大鼠在中等浓度甲醛环境暴露下肺VR 1和管家基因G 3PD表达水平。结果中等浓度甲醛3.0 m g/m3灌流比清洁空气灌流导致幼年大鼠肺VR 1-mRNA表达增加。结论中等浓度甲醛灌流导致幼年大鼠肺部VR 1-mRNA表达量增加。
基金Funding for this research was provided by the Australia Grain Research and Development Corporation(9176507)the Western Crop Genetics Alliance.Jingye Cheng thanks The University of Tasmania,Australia for the scholarship(495802)。
文摘Improved yield potential is the goal of barley domestication and cultivation.During this process,two-and six-rowed barley types emerged and have been utilised in breeding and production.The six-rowed type could produce three times as many grains as its ancestral two-rowed forms,thus dominating barley cultivation for thousands of years.The deficiens form of the two-rowed type,characterised by extremely suppressed lateral spikelets,has gained dominance over the past few decades in barley-growing regions worldwide.We hypothesised that the absence of lateral spikelets in deficiens barley affects spike architecture and spike-related traits,contributing to its superior yield potential of deficiens barley cultivation.Currently,a deficiens barley variety,RGT Planet,is the most popular barley variety in the world.In this study,we used two F_(2) populations derived from crossing RGT Planet with two canonical two-rowed barley and identified the functional allele Vrs1.t1 associated with deficiens morphology.We observed that the Vrs1.t1 allele may contribute to high yield potential by optimising spike architecture through increased spikelet length,grain number,and grain size.Phylogenetic analysis suggests that the deficiens mutation was likely present from the early stages of barley cultivation in the Fertile Crescent and spread to Ethiopia and beyond with agricultural expansion.We conclude that the ancient deficiens allele Vrs1.t1 has been a critical driver for the recent success of modern barley improvement by optimising spike architecture.
文摘最近,有两种接受伤害性热刺激的受体被克隆,分别命名为VR1(Vanilloid receptor subtype 1)和VRL1(Vanilloid receptor like protein 1)。本研究探讨了VR1作为原发性感觉神经元和伤害感受神经元可认识的肽P物质(SP)与降钙素基因关联肽(CGRP)能神经元的关系。
文摘目的:探索非致炎浓度的福尔马林对小鼠皮肤辣椒素受体(vanilloid receptor type 1,VR1)的激活作用。方法:选用KM小鼠随机分为3组:福尔马林(formalin,FM)组、辣椒素(capsaicin,CAP)组和Capsazepine(CPZ)组。采用注射后5 m in内小鼠舔舐注射脚掌的时间作为评价疼痛反应的指标,对各组小鼠的痛反应进行观测。结果:0.025%FM和200μmol/L CAP是理想的实验浓度,既能引起显著疼痛反应,又不引起炎性损伤的致痛浓度,适于研究受体拮抗剂的抗疼痛作用;1μmol/L CPZ分别与200μmol/L CAP和0.025%FM共注射能有效的拮抗CAP和FM引起的疼痛反应。结论:非致炎浓度FM所致的疼痛反应在外周可能由VR1介导。
