CAMS&PUMC,Beijing 100005,China;3 Medical Genetic Laboratory,Institute of Basic Medical Science,CAMS&PUMC,Kunming 650118,ChinaThis paper investigated the correlation between polymorphisms and haplotypes in the ...CAMS&PUMC,Beijing 100005,China;3 Medical Genetic Laboratory,Institute of Basic Medical Science,CAMS&PUMC,Kunming 650118,ChinaThis paper investigated the correlation between polymorphisms and haplotypes in the apolipoprotein B(apoB)gene(SP-I/D,XbaI-RFLP,VNTR)and natural longevity persons among the Uygur people in Xin-jiang.For this purpose,191 healthy Uygur individuals aged above 90 from Hetian area of Xinjiang were recruited,and another 53 persons aged 65—70 from the same nationality,the same region and with the same gender ratio,served as the control group.Genotyping was performed by PCR-SSP,PCR-RFLP and PCR-sequencing methods.Logistic regression analyses revealed that the frequencies of X+X+genotype,M and L alleles and the genetypes composed of M and L were significantly higher in the longevity group than in the control group.In haplotype analyses,we found that,in the long-lived people,the frequency of haplotypes composed of the X+and M alleles was significantly higher whereas the frequency of haplotypes composed of the X-and S alleles was significantly lower(both P<0.05)I than those of their controls.These results indicated that the S allele,SS genotype and X+-S,D-S,D-X+-S haplotypes were the possible adverse factors,whereas the M,L alleles,X+X+,MM,ML,LL genotypes and I-X+-M,X+-M haplotypes were the possibe protective factors for the naturally long-lived Uygur people in China.展开更多
文摘CAMS&PUMC,Beijing 100005,China;3 Medical Genetic Laboratory,Institute of Basic Medical Science,CAMS&PUMC,Kunming 650118,ChinaThis paper investigated the correlation between polymorphisms and haplotypes in the apolipoprotein B(apoB)gene(SP-I/D,XbaI-RFLP,VNTR)and natural longevity persons among the Uygur people in Xin-jiang.For this purpose,191 healthy Uygur individuals aged above 90 from Hetian area of Xinjiang were recruited,and another 53 persons aged 65—70 from the same nationality,the same region and with the same gender ratio,served as the control group.Genotyping was performed by PCR-SSP,PCR-RFLP and PCR-sequencing methods.Logistic regression analyses revealed that the frequencies of X+X+genotype,M and L alleles and the genetypes composed of M and L were significantly higher in the longevity group than in the control group.In haplotype analyses,we found that,in the long-lived people,the frequency of haplotypes composed of the X+and M alleles was significantly higher whereas the frequency of haplotypes composed of the X-and S alleles was significantly lower(both P<0.05)I than those of their controls.These results indicated that the S allele,SS genotype and X+-S,D-S,D-X+-S haplotypes were the possible adverse factors,whereas the M,L alleles,X+X+,MM,ML,LL genotypes and I-X+-M,X+-M haplotypes were the possibe protective factors for the naturally long-lived Uygur people in China.
