Cervical cancer is a prevalent gynecological malignancy,with approximately 90%of cases attributed to human papillomavirus(HPV)infection.Rapid and accurate nucleic acid detection is one of the leading methods to improv...Cervical cancer is a prevalent gynecological malignancy,with approximately 90%of cases attributed to human papillomavirus(HPV)infection.Rapid and accurate nucleic acid detection is one of the leading methods to improving screening coverage for early cervical cancer diagnosis.However,most existing techniques are usually complex and require expensive instrumentation.Clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated systems have great advantages in nucleic acid detection.We herein combined the CRISPR-Cas12a with a universal dual-mode fluorescent nanoparticles(FNPs)platform to construct a highly sensitive signal-offassay for HPV high-risk subtypes detection.The signal readout module uses a single-stranded DNA linker,which forms a sandwich structure with DNA-functionalized magnetic beads and DNA-functionalized FNPs to generate signals.If trans-cleavage activity was activated by the targets,the linker was consumed and therefore could not form the sandwich structure to produce signals.HPV16 and HPV18 as model targets,the limits of detection as low as 5 pmol/L were successfully achieved without amplification.We validated the feasibility of the real-sample detection using HPV16 and HPV18 pseudo viruses.The proposed method can be easily adapted for other virus or bacterial assays by modifying the CRISPR-derived RNA(crRNA),which shows great potential for clinical diagnosis.展开更多
The dynamic coupling effects on fusion cross sections for reactions^(32)S + ^(94,96)Zr and ^(40)Ca + ^(94,96)Zr are studied with the universal fusion function formalism and an empirical coupled channel(ECC) model. An ...The dynamic coupling effects on fusion cross sections for reactions^(32)S + ^(94,96)Zr and ^(40)Ca + ^(94,96)Zr are studied with the universal fusion function formalism and an empirical coupled channel(ECC) model. An examination of the reduced fusion functions shows that the total effect of couplings to inelastic excitations and neutron transfer channels on fusion in ^(32)S +^(94)Zr(^(40)Ca +^(94)Zr) is almost the same as that in ^(32)S +^(96)Zr(^(40)Ca +^(96)Zr). The enhancements of the fusion cross section at sub-barrier energies due to inelastic channel coupling and neutron transfer channel coupling are evaluated separately by using the ECC model. The results show that effect of couplings to inelastic excitations channels in the reactions with94 Zr as target should be similar as that in the reactions with ^(96) Zr as target. This implies that the quadrupole deformation parameters β_2of ^(94)Zr and^(96) Zr should be similar to each other.However, β_2 's predicted from the finite-range droplet model, which are used in the ECC model, are quite different. Experiments on^(48) Ca +^(94)Zr or^(36) S +^(94)Zr are suggested to solve the puzzling issue concerning β_2for^(94)Zr.展开更多
基金financially supported by the National Natural Science Foundation of China(22004005)Special Project on Biomedical Innovation(223777118D)。
文摘Cervical cancer is a prevalent gynecological malignancy,with approximately 90%of cases attributed to human papillomavirus(HPV)infection.Rapid and accurate nucleic acid detection is one of the leading methods to improving screening coverage for early cervical cancer diagnosis.However,most existing techniques are usually complex and require expensive instrumentation.Clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated systems have great advantages in nucleic acid detection.We herein combined the CRISPR-Cas12a with a universal dual-mode fluorescent nanoparticles(FNPs)platform to construct a highly sensitive signal-offassay for HPV high-risk subtypes detection.The signal readout module uses a single-stranded DNA linker,which forms a sandwich structure with DNA-functionalized magnetic beads and DNA-functionalized FNPs to generate signals.If trans-cleavage activity was activated by the targets,the linker was consumed and therefore could not form the sandwich structure to produce signals.HPV16 and HPV18 as model targets,the limits of detection as low as 5 pmol/L were successfully achieved without amplification.We validated the feasibility of the real-sample detection using HPV16 and HPV18 pseudo viruses.The proposed method can be easily adapted for other virus or bacterial assays by modifying the CRISPR-derived RNA(crRNA),which shows great potential for clinical diagnosis.
基金supported by the National Key Basic Research Program of China(Grant No.2013CB834400)the National Natural Science Foundation of China(Grant Nos.11175252+4 种基金111201010051127524811475115and 11525524)the Knowledge Innovation Project of the Chinese Academy of Sciences(Grant No.KJCX2-EW-N01)
文摘The dynamic coupling effects on fusion cross sections for reactions^(32)S + ^(94,96)Zr and ^(40)Ca + ^(94,96)Zr are studied with the universal fusion function formalism and an empirical coupled channel(ECC) model. An examination of the reduced fusion functions shows that the total effect of couplings to inelastic excitations and neutron transfer channels on fusion in ^(32)S +^(94)Zr(^(40)Ca +^(94)Zr) is almost the same as that in ^(32)S +^(96)Zr(^(40)Ca +^(96)Zr). The enhancements of the fusion cross section at sub-barrier energies due to inelastic channel coupling and neutron transfer channel coupling are evaluated separately by using the ECC model. The results show that effect of couplings to inelastic excitations channels in the reactions with94 Zr as target should be similar as that in the reactions with ^(96) Zr as target. This implies that the quadrupole deformation parameters β_2of ^(94)Zr and^(96) Zr should be similar to each other.However, β_2 's predicted from the finite-range droplet model, which are used in the ECC model, are quite different. Experiments on^(48) Ca +^(94)Zr or^(36) S +^(94)Zr are suggested to solve the puzzling issue concerning β_2for^(94)Zr.
