A method for producing size- and shape-con-trolled calcium alginate beads with immobilized proteins was developed. Unlike previous cal-cium alginate bead production methods, pro-tein-immobilized alginate beads with un...A method for producing size- and shape-con-trolled calcium alginate beads with immobilized proteins was developed. Unlike previous cal-cium alginate bead production methods, pro-tein-immobilized alginate beads with uniform shape and sizes less then 20 micrometers in diameter could successfully be produced by using sonic vibration. BSA and FITC-conjugated anti-BSA antibodies were used to confirm pro-tein immobilization in the alginate beads. Pro-tein diffusion from the beads could be reduced to less than 10% by cross-linking the proteins to the alginate with 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC) and N-hydroxysul-fosuccinimide (NHSS). The calcium alginate beads could also be arranged freely on a slide glass by using a femtosecond laser.展开更多
This study investigated multi-response optimization of the pulse metal active gas-tungsten inert gas( PMAG-TIG) twin arc hybrid root welding process for an optimal parametric combination to yield favorable back bead g...This study investigated multi-response optimization of the pulse metal active gas-tungsten inert gas( PMAG-TIG) twin arc hybrid root welding process for an optimal parametric combination to yield favorable back bead geometry of welded joints using grey relational analysis and Taguchi method.Eighteen experimental runs based on an orthogonal array following the Taguchi method were performed to derive objective functions to be optimized within the experimental domain.The objective functions were selected in relation to parameters of PMAG-TIG twin arc root welding back bead geometry: back bead width to root reinforcement ratio and deposited metal height.The Taguchi approach was followed by grey relational analysis to solve the multi-response optimization problem.The significance of factors on overall quality characteristics of the weld joint was also evaluated quantitatively using analysis of variance.Optimal results were verified through additional experiments,and showed to feasibility of applying grey relation analysis in combination with Taguchi technique for continuous improvement of product quality in the manufacturing industry.展开更多
Laser welding (LW) becomes one of the most economical high quality joining processes. LW offers the advantage of very controlled heat input resulting in low distortion and the ability to weld heat sensitive components...Laser welding (LW) becomes one of the most economical high quality joining processes. LW offers the advantage of very controlled heat input resulting in low distortion and the ability to weld heat sensitive components. To exploit efficiently the benefits presented by LW, it is necessary to develop an integrated approach to identify and control the welding process variables in order to produce the desired weld characteristics without being forced to use the traditional and fastidious trial and error procedures. The paper presents a study of weld bead geometry characteristics prediction for laser overlap welding of low carbon galvanized steel using 3D numerical modelling and experimental validation. The temperature dependent material properties, metallurgical transformations and enthalpy method constitute the foundation of the proposed modelling approach. An adaptive 3D heat source is adopted to simulate both keyhole and conduction mode of the LW process. The simulations are performed using 3D finite element model on commercial software. The model is used to estimate the weld bead geometry characteristics for various LW parameters, such as laser power, welding speed and laser beam diameter. The calibration and validation of the 3D numerical model are based on experimental data achieved using a 3 kW Nd:Yag laser system, a structured experimental design and confirmed statistical analysis tools. The results reveal that the modelling approach can provide not only a consistent and accurate prediction of the weld characteristics under variable welding parameters and conditions but also a comprehensive and quantitative analysis of process parameters effects on the weld quality. The results show great concordance between predicted and measured values for weld bead geometry characteristics, such as depth of penetration, bead width at the top surface and bead width at the interface between sheets, with an average accuracy greater than 95%.展开更多
This project work focuses on the reduction of weld undercuts using the Taguchi method. The phenomenon of weld undercuts constitutes a major problem for the welding industry. When undercuts occur, and particularly when...This project work focuses on the reduction of weld undercuts using the Taguchi method. The phenomenon of weld undercuts constitutes a major problem for the welding industry. When undercuts occur, and particularly when such cuts are deep, it has a negative impact on the weld as it lowers the integrity and quality of the weldment. Therefore, efforts are made globally to reduce the depth of such weld undercuts to the barest minimum. Several optimization methods have been adopted;however, in this study, the Taguchi method is applied. “The smaller the better components” of the Taguchi method is applied. From the results obtained from applying this Taguchi method, the optimum process parameters obtained are A2-B1-C2, which are a voltage of 20 V, a current of 180 A, and a welding speed of 130 mm/s, required to form an undercut of 0.03 mm. Whereas the existing process parameters used by the company are A1-B3-C, which make an undercut to a depth of 0.09 mm. It is concluded that the use of Taguchi method has been able to reduce the depth of undercut as shown in this study. A step-by-step approach is presented in the study.展开更多
文摘A method for producing size- and shape-con-trolled calcium alginate beads with immobilized proteins was developed. Unlike previous cal-cium alginate bead production methods, pro-tein-immobilized alginate beads with uniform shape and sizes less then 20 micrometers in diameter could successfully be produced by using sonic vibration. BSA and FITC-conjugated anti-BSA antibodies were used to confirm pro-tein immobilization in the alginate beads. Pro-tein diffusion from the beads could be reduced to less than 10% by cross-linking the proteins to the alginate with 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC) and N-hydroxysul-fosuccinimide (NHSS). The calcium alginate beads could also be arranged freely on a slide glass by using a femtosecond laser.
基金supported by the National Natural Science Foundation of China(Grant No.11375038)Science Fund for Creative Research Groups of NSFC(Grant No.51621064)
文摘This study investigated multi-response optimization of the pulse metal active gas-tungsten inert gas( PMAG-TIG) twin arc hybrid root welding process for an optimal parametric combination to yield favorable back bead geometry of welded joints using grey relational analysis and Taguchi method.Eighteen experimental runs based on an orthogonal array following the Taguchi method were performed to derive objective functions to be optimized within the experimental domain.The objective functions were selected in relation to parameters of PMAG-TIG twin arc root welding back bead geometry: back bead width to root reinforcement ratio and deposited metal height.The Taguchi approach was followed by grey relational analysis to solve the multi-response optimization problem.The significance of factors on overall quality characteristics of the weld joint was also evaluated quantitatively using analysis of variance.Optimal results were verified through additional experiments,and showed to feasibility of applying grey relation analysis in combination with Taguchi technique for continuous improvement of product quality in the manufacturing industry.
文摘Laser welding (LW) becomes one of the most economical high quality joining processes. LW offers the advantage of very controlled heat input resulting in low distortion and the ability to weld heat sensitive components. To exploit efficiently the benefits presented by LW, it is necessary to develop an integrated approach to identify and control the welding process variables in order to produce the desired weld characteristics without being forced to use the traditional and fastidious trial and error procedures. The paper presents a study of weld bead geometry characteristics prediction for laser overlap welding of low carbon galvanized steel using 3D numerical modelling and experimental validation. The temperature dependent material properties, metallurgical transformations and enthalpy method constitute the foundation of the proposed modelling approach. An adaptive 3D heat source is adopted to simulate both keyhole and conduction mode of the LW process. The simulations are performed using 3D finite element model on commercial software. The model is used to estimate the weld bead geometry characteristics for various LW parameters, such as laser power, welding speed and laser beam diameter. The calibration and validation of the 3D numerical model are based on experimental data achieved using a 3 kW Nd:Yag laser system, a structured experimental design and confirmed statistical analysis tools. The results reveal that the modelling approach can provide not only a consistent and accurate prediction of the weld characteristics under variable welding parameters and conditions but also a comprehensive and quantitative analysis of process parameters effects on the weld quality. The results show great concordance between predicted and measured values for weld bead geometry characteristics, such as depth of penetration, bead width at the top surface and bead width at the interface between sheets, with an average accuracy greater than 95%.
文摘This project work focuses on the reduction of weld undercuts using the Taguchi method. The phenomenon of weld undercuts constitutes a major problem for the welding industry. When undercuts occur, and particularly when such cuts are deep, it has a negative impact on the weld as it lowers the integrity and quality of the weldment. Therefore, efforts are made globally to reduce the depth of such weld undercuts to the barest minimum. Several optimization methods have been adopted;however, in this study, the Taguchi method is applied. “The smaller the better components” of the Taguchi method is applied. From the results obtained from applying this Taguchi method, the optimum process parameters obtained are A2-B1-C2, which are a voltage of 20 V, a current of 180 A, and a welding speed of 130 mm/s, required to form an undercut of 0.03 mm. Whereas the existing process parameters used by the company are A1-B3-C, which make an undercut to a depth of 0.09 mm. It is concluded that the use of Taguchi method has been able to reduce the depth of undercut as shown in this study. A step-by-step approach is presented in the study.
