This editorial discusses a case report recently published in the World Journal of Clinical Cases.The report describes the clinical presentation,imaging,diagnosis,and treatment of a patient with tuberous sclerosis comp...This editorial discusses a case report recently published in the World Journal of Clinical Cases.The report describes the clinical presentation,imaging,diagnosis,and treatment of a patient with tuberous sclerosis complex(TSC)combined with primary lymphedema(PLE).Additionally,it retrospectively analyzes the data of 16 previously reported cases of children with TSC combined with PLE to summarize the epidemiology,genetic diagnosis,and current main treatments of these patients.The report also speculates on the pathological and physiological mechanisms underlying TSC combined with PLE.TSC combined with PLE is rare;therefore,the report provides a theoretical basis for understanding the pathophysiological mechanisms and treatment options for patients with TSC and PLE.Comprehensive clinical management of TSC is essential due to the diverse and multiorgan nature of its manifestations,often requiring a multidisciplinary approach for newly diagnosed cases.展开更多
The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for lit...The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for literature that brought the observed aspects to the structuring of the technical sheets, i.e., literature that contains data related to composition, active ingredients, interaction (when described in the consulted literature), health condition in which they may be useful in helath care by various health professionals and anyone who may be of interest. The data obtained shows that there is a need for a careful evaluation of the foods (that contain roots, rhizomes, or tubers) used by polymedicated people due to possible interactions and incompatibilities that may exist.展开更多
Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breedin...Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breeding.In this study,based on the primary mapping of the tuber eyedepth locus using a small primary-segregating population,a large secondary-segregating population with 2100 individuals was used to map the eye-depth locus further.A major quantitative trait locus for eye-depth on chromosome 10 was identified(designated qEyd10.1)using BSAseq and traditional QTL mapping methods.The qEyd10.1 could explain 55.0%of the eye depth phenotypic variation and was further narrowed to a 309.10 kb interval using recombinant analysis.To predict candidate genes,tissue sectioning and RNA-seq of the specific tuber tissues were performed.Genes encoding members of the peroxidase superfamily with likely roles in indole acetic acid regulation were considered the most promising candidates.These results will facilitate marker-assisted selection for the shallow-eye trait in potato breeding and provide a solid basis for eye-depth gene cloning and the analysis of tuber eye-depth regulatory mechanisms.展开更多
An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) ...An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) was carried out during 2004 to assess the effect of saline aquaculture effluent on plant growth and soil properties in the Laizhou region, Shandong Province, China and to determine an optimal salinity threshold for aquaculture effluent. Cumulative evapotranspiration for the saline aquaculture effluent irrigation and non-irrigation treatments was lower than that for the freshwater irrigation treatment. Soil electrical conductivity was higher with respect to saline aquaculture effluent irrigation treatment compared to that with respect to non-irrigation or freshwater irrigation treatment. For Jerusalem artichoke (Helianthus tuberosus L.), in comparison to the freshwater treatment, plant height and aboveground biomass for the 1:3 and 1:4 treatments were constrained, whereas stem width and root biomass were enhanced. Concomitantly, higher tuber yield was obtained for the 1:3 and 1:4 treatments compared to that for CK1 and 1:1 treatments. Nitrogen and phosphorus were higher in tubers of the 1:4 treatment. This study demonstrated that saline aquaculture effluent could be used successfully to irrigate Jerusalem artichoke with higher tuber yield and nutrient removal.展开更多
According to previous analysis, some properties bounding up with tuber yield were investigated. The results showed that tuber average weight, plastid Mg2 + -ATPase activity, plastid Ca2 + -ATPase activity, mitochondri...According to previous analysis, some properties bounding up with tuber yield were investigated. The results showed that tuber average weight, plastid Mg2 + -ATPase activity, plastid Ca2 + -ATPase activity, mitochondria Mg2 + -ATPase activity, total soluble protein content, tuber average diameter, and Q-enzyme activity were important factors determining the tuber yield. The linear regression equation was:Y = 0.5211 + 0.0595X(1)+0.8389X(2) +0.0882X(3) -0.0073X(4) +0.1449X(5) +0.3510X(6) +0.0031X(7) -0.00003X(8) + 0.3412X(9) + 0.0127X(10) + 0.2904X(ll) + 0.0570X(12) + 0.0159X(13) + 0.3585X(14) + 0.0134X(15) - 0.1012X(16). At the same time, the relation between several important properties and soluble protein fractions were analyzed.展开更多
[Objective] This study aimed to investigate the appropriate dose of 60^Co-γ irradiation for tubers of purple sweet potato.[Methods] The tubers of two purple sweet potato varieties Xuzi L-7 and Yuenanzi were used as e...[Objective] This study aimed to investigate the appropriate dose of 60^Co-γ irradiation for tubers of purple sweet potato.[Methods] The tubers of two purple sweet potato varieties Xuzi L-7 and Yuenanzi were used as experimental materials,and treated with 60^Co-γ irradiation at different doses（0,25,50,75,100,125 and 150 Gy） to determine the emergence rate,number of emerged seedlings per tuber and fresh weight per seedling.[Results] After the 60^Co-γ irradiation at different doses,the emergence period of tubers was postponed while the emergence rate,number of emerged seedlings per tuber and fresh weight per plant were decreased,and the differences between different varieties and different irradiation doses reached significance level.Compared with the control,the emergence period of tubers at irradiation doses above 25 Gy were postponed by 2,3,5,6 and 12 d,respectively.[Conclusion] The half lethal dose（LD50） of Xuzi L-7 was 66.73 Gy,and that of Yuenanzi was 74.96 Gy.展开更多
Wild-type potato (Solanum tuberosum L.) plants and their transformants harboring agrobacterial rolB or rolC genes under control of the patatin class I promoter were cultured in vitro. These plants were used as a sourc...Wild-type potato (Solanum tuberosum L.) plants and their transformants harboring agrobacterial rolB or rolC genes under control of the patatin class I promoter were cultured in vitro. These plants were used as a source of single-node stem cuttings. The structure of native starch in tubers formed on cuttings was determined using methods of X-ray scattering and differential scanning microcalorimetry (DSC). It was found that in starch from tubers of rolB plants the melting temperature of crystalline lamella was lower and their thickness was less than that in wild-type potato. In tubers of rolC plants starch differed from starch in wild-type plants by a higher melting temperature, reduced melting enthalpy, and a greater thickness of crystalline lamellae. The melting of starch from tubers of rolC plants proceeded as the melting of two independent crystalline structures with melting temperatures of 338.0°K and 342.8°K. Overall data show that starches of different structure can be obtained by using transgenic approach.展开更多
Dehydration,viability,proline,sucrose and glucose concentrations were evaluated on green and white microtubers during storage at two different temperatures.After 10 months at 4℃,the green and white microtubers showed...Dehydration,viability,proline,sucrose and glucose concentrations were evaluated on green and white microtubers during storage at two different temperatures.After 10 months at 4℃,the green and white microtubers showed shrinkage with a dry weight loss of 3.91%and 3.15%.Both,the green and white microtubers at 4℃ presented an enhanced sprouting after storage.At 23℃,the green microtubers lost the lowest quantity in dry weight(0.8%)and white microtubers lost 2.2%.This behavior is possibly related to the increase in the thickness of the peridermis observed in green microtubers or to the osmotic regulation mediated principally by the observed concentrations of proline and glucose but not sucrose.The best storage conditions for potato microtubers obtained in vitro were at 4℃ for green or white microtubers for up to 10 months with little loss of viability.展开更多
Field trials to determine the yield response of potatoes to Umostart Super Zn （USZ） fertilizer （11.46.0 ＋ 2 Zn ＋ Humates） were carried out in 2004 using the cultivar Tigoni. During the long rains season, the tre...Field trials to determine the yield response of potatoes to Umostart Super Zn （USZ） fertilizer （11.46.0 ＋ 2 Zn ＋ Humates） were carried out in 2004 using the cultivar Tigoni. During the long rains season, the treatments included USZ at 15 kg ha^-1 at planting; 30 kg ha^-1 split into 15 kg ha^-1 at planting and 15 kg ha^-1 during final earthing-up; 30 kg ha^-1 at planting; and 45 kg ha^-1 at planting. Treatments during the short rains included USZ at 30 kg ha^-1 split into 15 kg ha^-1 at planting and 15 kg ha^-1 during final earthing-up; 30 kg ha^-1 at planting; 40 kg ha^-1 at planting; 50 kg ha^-1 at planting; 50 kg ha^-1 split into 25 kg ha^-1 at planting and 25 kg ha^-1 during final earthing-up; and 60 kg ha^-1 at planting. USZ was contrasted with Diammonium phosphate （DAP） fertilizer （18：46：0） at 300 kg ha^-1 and 500 kg ha^-1 applied at planting and a control in which no fertilizer was applied. The DAP 300 kg ha^-1 treatment was however, not included during the long rains season. The highest tuber yields among the USZ treatments were obtained in plots treated with 45 kg ha^-1 （27.11 t ha^-1） during the long rains and 60 kg ha^-1 （55.17 t ha^-1） during the short rains season. Application of USZ resulted in a yield increase of at least 3.90 and 19.06 t ha^-1 during the long and short rains seasons respectively.展开更多
Starch degradation in cells is closely associated with cereal seed germination, photosynthesis in leaves, carbohydrate storage in tuber and tuberous roots, and fleshy fruit development. Based on previously reported ...Starch degradation in cells is closely associated with cereal seed germination, photosynthesis in leaves, carbohydrate storage in tuber and tuberous roots, and fleshy fruit development. Based on previously reported in vitro assays, β amylase is considered as one of the key enzymes catalyzing starch breakdown, but up to date its role in starch breakdown in living cells remains unclear because the enzyme was shown often extrachloroplastic in living cells. Recently we have shown for the first time that β_amylase is predominantly immuno_localized to plastids in living cells of developing apple fruit. But it remains to know whether this model of β_amylase compartmentation is more widespread in plant living cells. The present experiment, conducted in tuberous root of sweet potato ( Ipomea batatas Lam. cv. Xushu 18) and via immunogold electron_microscopy technique, showed that β amylase visualized by gold particles was predominantly localized in plastids especially at periphery of starch granules, but the gold particles were scarcely found in other subcellular compartments, indicating that the enzyme is subcellularly compartmented in the same zone as its starch substrates. The density of gold particles (β amylase) in plastids was increasing during growing season, but the predominantly plastid_distributed pattern of β amylase in cells was shown unchanged throughout the tuberous root development. These data prove that the enzyme is compartmented in its functional sites, and so provide evidence to support the possible widespread biological function of the enzyme in catalyzing starch breakdown in plant living cells or at least in living cells of plant storage organs.展开更多
In order to know the cross reaction betweenBCG and Atypical Myeobaeteria(AM)anti-.genieity,1150 BCG immunized babies aged 12—24 weeks,undertook a bilateral arm control testwith H-PPD type 11 AM-PPD and BCG-PPD.The re...In order to know the cross reaction betweenBCG and Atypical Myeobaeteria(AM)anti-.genieity,1150 BCG immunized babies aged 12—24 weeks,undertook a bilateral arm control testwith H-PPD type 11 AM-PPD and BCG-PPD.The results show that all the AM—PPDtested can bring about a delayed allergy in thesebabies,but the total positive rate(42.87%)and mean reactive diameter(3.87mm)are thelowest and smallest among the above mentionedthree types of PPD.展开更多
According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac effica...According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac efficacy of alkaloids in Aconitum tubers. First, the chemical ingredients in unprocessed and processed Aconitum tubers were identified and compared by using high performance liquid chromatography time-of-flight mass spectrometry(HPLC-TOF/MS) and multivariate pattern recognition methods. Then the representative alkaloids in Aconitum tubers, aconitine, benzoylaconine, and aconine, which belong to diester-diterpenoid alkaloids,monoester-diterpenoid alkaloids, and amine-diterpenoid alkaloids, respectively, were selected for further validation of attenuated mechanism. Subsequent pharmacological experiments with aconitine, benzoylaconine,and aconine in SD rats were used to validate the effect of processing on cardiac functions. After processing the Aconitum tubers, it was found that the contents of diester-diterpenoid alkaloids were reduced, and those of monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids were increased, suggesting that diesterditerpenoid alkaloids were transformed into monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids.Through further decocting the aconitine in boiling water, it was confirmed that the three alkaloids could be progressively transformed. Pharmacological experiments with aconitine, benzoylaconine, and aconine in SD rats showed that aconitine at a dose of 0.01 mg/kg and aconine at a dose of 10 mg/kg enhanced the cardiac function, while benzoylaconine at a dose of 2 mg/kg weakened the cardiac function. The effect of processing is attributed to the transformation of the most toxic diester-diterpenoid alkaloids into less toxic monoesterditerpenoid alkaloids and amine-diterpenoid alkaloids.展开更多
The turnip(Brassica rapa var. rapa) is a biennial crop that is planted in late summer/early fall and forms fleshy tubers for food in temperate regions. The harvested tubers then overwinter and are planted again the ne...The turnip(Brassica rapa var. rapa) is a biennial crop that is planted in late summer/early fall and forms fleshy tubers for food in temperate regions. The harvested tubers then overwinter and are planted again the next spring for flowering and seeds. FLOWERING LOCUS C(FLC) is a MADS-box transcription factor that acts as a major repressor of floral transition by suppressing the flowering promoters FT and SOC1. Here we show that vernalization effectively represses tuber formation and promotes flowering in Tibetan turnip. We functionally characterized four FLC homologues(BrrFLC1,FLC2, FLC3, and FLC5), and found that BrrFLC2 and BrrFLC1 play a major role in repressing flowering in turnip and in transgenic Arabidopsis. In contrast, tuber formation was correlated with BrrFLC1 expression in the hypocotyl and was repressed under cold treatment following the quantitative downregulation of BrrFLC1. Grafting experiments of non-vernalized and vernalized turnips revealed that vernalization independently suppressed tuberization in the tuber or hypocotyl of the rootstock or scion, which occurred in parallel with the reduction in BrrFLC1 activity. Together, our results demonstrate that the Tibetan turnip is highly responsive to cold exposure, which is associated with the expression levels of BrrFLC genes.展开更多
A new C-18 phytosphingosine derivative (1) was isolated from the fruiting bodies of Tuber indicum. Its structure was established as (2S, 3S, 4R, 2R)-2-N-(2-hydroxytricosano- yl)-octadecan-1, 3, 4-triol by spectral a...A new C-18 phytosphingosine derivative (1) was isolated from the fruiting bodies of Tuber indicum. Its structure was established as (2S, 3S, 4R, 2R)-2-N-(2-hydroxytricosano- yl)-octadecan-1, 3, 4-triol by spectral and chemical methods.展开更多
China is an important domestication center of yams,and two main yam species of Dioscorea opposita and D.alata are commonly cultivated in China.However,the differences of nutritional and medicinal characteristics betwe...China is an important domestication center of yams,and two main yam species of Dioscorea opposita and D.alata are commonly cultivated in China.However,the differences of nutritional and medicinal characteristics between the two species and their subgroups remain unclear,which would greatly affect the resource conservation and commercial utilization of yams.In this study,typical yam resources including the species of D.opposita(wild and cultivated Ruichang yam from southern China,and Tiegun yam from northern China)and two landraces of D.alata(Longyan yam and Anyuan yam from southern China)were selected as materials.Nutritional traits and medicinal characteristics were determined and analyzed respectively.The results showed that there was no significant differences in the content of most nutrients between D.opposita and D.alata,but most cultivated Ruichang yam of D.opposita showed higher levels of starch,soluble sugar,sucrose,and ascorbate in tuber than that in yam from D.alata.Moreover,an UPLC-MS method was developed for identification and determination of medicinal characteristics in the two species.The results showed that allantoin can be detected in all selected samples.Cultivated Ruichang yam of D.opposita possessed the highest allantoin content among the tested materials,and was significantly different with that in Tiegun yam and D.alata.Dioscin was not detected in D.alata.Overall,there was little difference in nutritional composition between D.opposita and D.alata,but the medicinal quality of D.opposita was better than that of D.alata.Due to the outstanding comprehensive quality,the local variety of cultivated Ruichang yam can be further developed and utilized.展开更多
文摘This editorial discusses a case report recently published in the World Journal of Clinical Cases.The report describes the clinical presentation,imaging,diagnosis,and treatment of a patient with tuberous sclerosis complex(TSC)combined with primary lymphedema(PLE).Additionally,it retrospectively analyzes the data of 16 previously reported cases of children with TSC combined with PLE to summarize the epidemiology,genetic diagnosis,and current main treatments of these patients.The report also speculates on the pathological and physiological mechanisms underlying TSC combined with PLE.TSC combined with PLE is rare;therefore,the report provides a theoretical basis for understanding the pathophysiological mechanisms and treatment options for patients with TSC and PLE.Comprehensive clinical management of TSC is essential due to the diverse and multiorgan nature of its manifestations,often requiring a multidisciplinary approach for newly diagnosed cases.
文摘The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for literature that brought the observed aspects to the structuring of the technical sheets, i.e., literature that contains data related to composition, active ingredients, interaction (when described in the consulted literature), health condition in which they may be useful in helath care by various health professionals and anyone who may be of interest. The data obtained shows that there is a need for a careful evaluation of the foods (that contain roots, rhizomes, or tubers) used by polymedicated people due to possible interactions and incompatibilities that may exist.
基金funded by the National Natural Science Foundation of China(Grant No.31801421)the Chinese Academy of Agricultural Sciences Innovation Project(Grant No.CAAS-ASTIPIVFCAAS).
文摘Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breeding.In this study,based on the primary mapping of the tuber eyedepth locus using a small primary-segregating population,a large secondary-segregating population with 2100 individuals was used to map the eye-depth locus further.A major quantitative trait locus for eye-depth on chromosome 10 was identified(designated qEyd10.1)using BSAseq and traditional QTL mapping methods.The qEyd10.1 could explain 55.0%of the eye depth phenotypic variation and was further narrowed to a 309.10 kb interval using recombinant analysis.To predict candidate genes,tissue sectioning and RNA-seq of the specific tuber tissues were performed.Genes encoding members of the peroxidase superfamily with likely roles in indole acetic acid regulation were considered the most promising candidates.These results will facilitate marker-assisted selection for the shallow-eye trait in potato breeding and provide a solid basis for eye-depth gene cloning and the analysis of tuber eye-depth regulatory mechanisms.
基金Project supported by the National Natural Science Foundation of China (No. 30470331)
文摘An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) was carried out during 2004 to assess the effect of saline aquaculture effluent on plant growth and soil properties in the Laizhou region, Shandong Province, China and to determine an optimal salinity threshold for aquaculture effluent. Cumulative evapotranspiration for the saline aquaculture effluent irrigation and non-irrigation treatments was lower than that for the freshwater irrigation treatment. Soil electrical conductivity was higher with respect to saline aquaculture effluent irrigation treatment compared to that with respect to non-irrigation or freshwater irrigation treatment. For Jerusalem artichoke (Helianthus tuberosus L.), in comparison to the freshwater treatment, plant height and aboveground biomass for the 1:3 and 1:4 treatments were constrained, whereas stem width and root biomass were enhanced. Concomitantly, higher tuber yield was obtained for the 1:3 and 1:4 treatments compared to that for CK1 and 1:1 treatments. Nitrogen and phosphorus were higher in tubers of the 1:4 treatment. This study demonstrated that saline aquaculture effluent could be used successfully to irrigate Jerusalem artichoke with higher tuber yield and nutrient removal.
基金the National Natural Science Foundation(39370486) the Laboratory of Vegetative Genetics and Physiology inthe Agricultural Ministry of P.R.China.
文摘According to previous analysis, some properties bounding up with tuber yield were investigated. The results showed that tuber average weight, plastid Mg2 + -ATPase activity, plastid Ca2 + -ATPase activity, mitochondria Mg2 + -ATPase activity, total soluble protein content, tuber average diameter, and Q-enzyme activity were important factors determining the tuber yield. The linear regression equation was:Y = 0.5211 + 0.0595X(1)+0.8389X(2) +0.0882X(3) -0.0073X(4) +0.1449X(5) +0.3510X(6) +0.0031X(7) -0.00003X(8) + 0.3412X(9) + 0.0127X(10) + 0.2904X(ll) + 0.0570X(12) + 0.0159X(13) + 0.3585X(14) + 0.0134X(15) - 0.1012X(16). At the same time, the relation between several important properties and soluble protein fractions were analyzed.
基金Supported by "12th Five-Year Plan" Breeding Project of Crops and Live stock of Sichuan Province~~
文摘[Objective] This study aimed to investigate the appropriate dose of 60^Co-γ irradiation for tubers of purple sweet potato.[Methods] The tubers of two purple sweet potato varieties Xuzi L-7 and Yuenanzi were used as experimental materials,and treated with 60^Co-γ irradiation at different doses（0,25,50,75,100,125 and 150 Gy） to determine the emergence rate,number of emerged seedlings per tuber and fresh weight per seedling.[Results] After the 60^Co-γ irradiation at different doses,the emergence period of tubers was postponed while the emergence rate,number of emerged seedlings per tuber and fresh weight per plant were decreased,and the differences between different varieties and different irradiation doses reached significance level.Compared with the control,the emergence period of tubers at irradiation doses above 25 Gy were postponed by 2,3,5,6 and 12 d,respectively.[Conclusion] The half lethal dose（LD50） of Xuzi L-7 was 66.73 Gy,and that of Yuenanzi was 74.96 Gy.
文摘Wild-type potato (Solanum tuberosum L.) plants and their transformants harboring agrobacterial rolB or rolC genes under control of the patatin class I promoter were cultured in vitro. These plants were used as a source of single-node stem cuttings. The structure of native starch in tubers formed on cuttings was determined using methods of X-ray scattering and differential scanning microcalorimetry (DSC). It was found that in starch from tubers of rolB plants the melting temperature of crystalline lamella was lower and their thickness was less than that in wild-type potato. In tubers of rolC plants starch differed from starch in wild-type plants by a higher melting temperature, reduced melting enthalpy, and a greater thickness of crystalline lamellae. The melting of starch from tubers of rolC plants proceeded as the melting of two independent crystalline structures with melting temperatures of 338.0°K and 342.8°K. Overall data show that starches of different structure can be obtained by using transgenic approach.
基金Authors thank CONACYT for a grant to JGOM(No.169550,DEPI:A020026).
文摘Dehydration,viability,proline,sucrose and glucose concentrations were evaluated on green and white microtubers during storage at two different temperatures.After 10 months at 4℃,the green and white microtubers showed shrinkage with a dry weight loss of 3.91%and 3.15%.Both,the green and white microtubers at 4℃ presented an enhanced sprouting after storage.At 23℃,the green microtubers lost the lowest quantity in dry weight(0.8%)and white microtubers lost 2.2%.This behavior is possibly related to the increase in the thickness of the peridermis observed in green microtubers or to the osmotic regulation mediated principally by the observed concentrations of proline and glucose but not sucrose.The best storage conditions for potato microtubers obtained in vitro were at 4℃ for green or white microtubers for up to 10 months with little loss of viability.
文摘Field trials to determine the yield response of potatoes to Umostart Super Zn （USZ） fertilizer （11.46.0 ＋ 2 Zn ＋ Humates） were carried out in 2004 using the cultivar Tigoni. During the long rains season, the treatments included USZ at 15 kg ha^-1 at planting; 30 kg ha^-1 split into 15 kg ha^-1 at planting and 15 kg ha^-1 during final earthing-up; 30 kg ha^-1 at planting; and 45 kg ha^-1 at planting. Treatments during the short rains included USZ at 30 kg ha^-1 split into 15 kg ha^-1 at planting and 15 kg ha^-1 during final earthing-up; 30 kg ha^-1 at planting; 40 kg ha^-1 at planting; 50 kg ha^-1 at planting; 50 kg ha^-1 split into 25 kg ha^-1 at planting and 25 kg ha^-1 during final earthing-up; and 60 kg ha^-1 at planting. USZ was contrasted with Diammonium phosphate （DAP） fertilizer （18：46：0） at 300 kg ha^-1 and 500 kg ha^-1 applied at planting and a control in which no fertilizer was applied. The DAP 300 kg ha^-1 treatment was however, not included during the long rains season. The highest tuber yields among the USZ treatments were obtained in plots treated with 45 kg ha^-1 （27.11 t ha^-1） during the long rains and 60 kg ha^-1 （55.17 t ha^-1） during the short rains season. Application of USZ resulted in a yield increase of at least 3.90 and 19.06 t ha^-1 during the long and short rains seasons respectively.
文摘Starch degradation in cells is closely associated with cereal seed germination, photosynthesis in leaves, carbohydrate storage in tuber and tuberous roots, and fleshy fruit development. Based on previously reported in vitro assays, β amylase is considered as one of the key enzymes catalyzing starch breakdown, but up to date its role in starch breakdown in living cells remains unclear because the enzyme was shown often extrachloroplastic in living cells. Recently we have shown for the first time that β_amylase is predominantly immuno_localized to plastids in living cells of developing apple fruit. But it remains to know whether this model of β_amylase compartmentation is more widespread in plant living cells. The present experiment, conducted in tuberous root of sweet potato ( Ipomea batatas Lam. cv. Xushu 18) and via immunogold electron_microscopy technique, showed that β amylase visualized by gold particles was predominantly localized in plastids especially at periphery of starch granules, but the gold particles were scarcely found in other subcellular compartments, indicating that the enzyme is subcellularly compartmented in the same zone as its starch substrates. The density of gold particles (β amylase) in plastids was increasing during growing season, but the predominantly plastid_distributed pattern of β amylase in cells was shown unchanged throughout the tuberous root development. These data prove that the enzyme is compartmented in its functional sites, and so provide evidence to support the possible widespread biological function of the enzyme in catalyzing starch breakdown in plant living cells or at least in living cells of plant storage organs.
文摘In order to know the cross reaction betweenBCG and Atypical Myeobaeteria(AM)anti-.genieity,1150 BCG immunized babies aged 12—24 weeks,undertook a bilateral arm control testwith H-PPD type 11 AM-PPD and BCG-PPD.The results show that all the AM—PPDtested can bring about a delayed allergy in thesebabies,but the total positive rate(42.87%)and mean reactive diameter(3.87mm)are thelowest and smallest among the above mentionedthree types of PPD.
基金financially supported by the National Natural Science Foundation of China (81573396)Military Innovation Funding (16CXZ012)
文摘According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac efficacy of alkaloids in Aconitum tubers. First, the chemical ingredients in unprocessed and processed Aconitum tubers were identified and compared by using high performance liquid chromatography time-of-flight mass spectrometry(HPLC-TOF/MS) and multivariate pattern recognition methods. Then the representative alkaloids in Aconitum tubers, aconitine, benzoylaconine, and aconine, which belong to diester-diterpenoid alkaloids,monoester-diterpenoid alkaloids, and amine-diterpenoid alkaloids, respectively, were selected for further validation of attenuated mechanism. Subsequent pharmacological experiments with aconitine, benzoylaconine,and aconine in SD rats were used to validate the effect of processing on cardiac functions. After processing the Aconitum tubers, it was found that the contents of diester-diterpenoid alkaloids were reduced, and those of monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids were increased, suggesting that diesterditerpenoid alkaloids were transformed into monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids.Through further decocting the aconitine in boiling water, it was confirmed that the three alkaloids could be progressively transformed. Pharmacological experiments with aconitine, benzoylaconine, and aconine in SD rats showed that aconitine at a dose of 0.01 mg/kg and aconine at a dose of 10 mg/kg enhanced the cardiac function, while benzoylaconine at a dose of 2 mg/kg weakened the cardiac function. The effect of processing is attributed to the transformation of the most toxic diester-diterpenoid alkaloids into less toxic monoesterditerpenoid alkaloids and amine-diterpenoid alkaloids.
基金supported by the National Science Foundation of China(No.31500221,31590823 and 31601999)the West Light Foundation of the Chinese Academy of Sciences by XXK
文摘The turnip(Brassica rapa var. rapa) is a biennial crop that is planted in late summer/early fall and forms fleshy tubers for food in temperate regions. The harvested tubers then overwinter and are planted again the next spring for flowering and seeds. FLOWERING LOCUS C(FLC) is a MADS-box transcription factor that acts as a major repressor of floral transition by suppressing the flowering promoters FT and SOC1. Here we show that vernalization effectively represses tuber formation and promotes flowering in Tibetan turnip. We functionally characterized four FLC homologues(BrrFLC1,FLC2, FLC3, and FLC5), and found that BrrFLC2 and BrrFLC1 play a major role in repressing flowering in turnip and in transgenic Arabidopsis. In contrast, tuber formation was correlated with BrrFLC1 expression in the hypocotyl and was repressed under cold treatment following the quantitative downregulation of BrrFLC1. Grafting experiments of non-vernalized and vernalized turnips revealed that vernalization independently suppressed tuberization in the tuber or hypocotyl of the rootstock or scion, which occurred in parallel with the reduction in BrrFLC1 activity. Together, our results demonstrate that the Tibetan turnip is highly responsive to cold exposure, which is associated with the expression levels of BrrFLC genes.
基金supported by the Natural Science Foundation of Yunnan Province(2000B0066M)
文摘A new C-18 phytosphingosine derivative (1) was isolated from the fruiting bodies of Tuber indicum. Its structure was established as (2S, 3S, 4R, 2R)-2-N-(2-hydroxytricosano- yl)-octadecan-1, 3, 4-triol by spectral and chemical methods.
基金This work was supported by the earmarked fund for Jiangxi Agriculture Research System,China(JXARS-19)the Jiangxi Provincial Key Research and Development Project,China(20192BBF60005).
文摘China is an important domestication center of yams,and two main yam species of Dioscorea opposita and D.alata are commonly cultivated in China.However,the differences of nutritional and medicinal characteristics between the two species and their subgroups remain unclear,which would greatly affect the resource conservation and commercial utilization of yams.In this study,typical yam resources including the species of D.opposita(wild and cultivated Ruichang yam from southern China,and Tiegun yam from northern China)and two landraces of D.alata(Longyan yam and Anyuan yam from southern China)were selected as materials.Nutritional traits and medicinal characteristics were determined and analyzed respectively.The results showed that there was no significant differences in the content of most nutrients between D.opposita and D.alata,but most cultivated Ruichang yam of D.opposita showed higher levels of starch,soluble sugar,sucrose,and ascorbate in tuber than that in yam from D.alata.Moreover,an UPLC-MS method was developed for identification and determination of medicinal characteristics in the two species.The results showed that allantoin can be detected in all selected samples.Cultivated Ruichang yam of D.opposita possessed the highest allantoin content among the tested materials,and was significantly different with that in Tiegun yam and D.alata.Dioscin was not detected in D.alata.Overall,there was little difference in nutritional composition between D.opposita and D.alata,but the medicinal quality of D.opposita was better than that of D.alata.Due to the outstanding comprehensive quality,the local variety of cultivated Ruichang yam can be further developed and utilized.
