An experimental method for calibration of optical trap force upon cells by use of electrokinetic phenomena is demonstrated. An electronkinetic sample chamber system （ESCS） is designed instead of a common sample cham...An experimental method for calibration of optical trap force upon cells by use of electrokinetic phenomena is demonstrated. An electronkinetic sample chamber system （ESCS） is designed instead of a common sample chamber and a costly automatism stage, thus the experimental setup is simpler and cheaper. Experiments indicate that the range of the trap force measured by this method is piconewton and sub-piconewton, which makes it fit for study on non-damage interaction between light and biological particles with optical tweezers especially. Since this method is relevant to particle electric charge, by applying an alternating electric field, the new method may overcome the problem of correcting drag force and allow us to measure simultaneously optical trap stiffness and particle electric charge.展开更多
In this paper, we combine the direct-forcing fictitious domain （DF/FD） method and the sharp interface method to resolve the problem of particle dielectrophoresis in two dimensions. The flow field and the motion of p...In this paper, we combine the direct-forcing fictitious domain （DF/FD） method and the sharp interface method to resolve the problem of particle dielectrophoresis in two dimensions. The flow field and the motion of particles are solved with the DF/FD method, the electric field is solved with the sharp inter- face method, and the electrostatic force on the particles is computed using the Maxwell stress tensor method. The proposed method is validated via three problems： effective conductivity of particle compos- ite between two planar plates, cell trapping in a channel, and motion of particles due to both conventional and traveling wave dielectrophoretic forces.展开更多
基金This work was supported by the National Natural Science Foundation of China under Grant No. 60378018 and 60578026.
文摘An experimental method for calibration of optical trap force upon cells by use of electrokinetic phenomena is demonstrated. An electronkinetic sample chamber system （ESCS） is designed instead of a common sample chamber and a costly automatism stage, thus the experimental setup is simpler and cheaper. Experiments indicate that the range of the trap force measured by this method is piconewton and sub-piconewton, which makes it fit for study on non-damage interaction between light and biological particles with optical tweezers especially. Since this method is relevant to particle electric charge, by applying an alternating electric field, the new method may overcome the problem of correcting drag force and allow us to measure simultaneously optical trap stiffness and particle electric charge.
基金support from the National Natural Science Foundation of China(no.10872181)the National Basic Research Program of China(no.2006CB705400)+1 种基金Chinese Universities Scientific Fundthe Major Program of the National Natural Science Foundation of China(no.10632070)
文摘In this paper, we combine the direct-forcing fictitious domain （DF/FD） method and the sharp interface method to resolve the problem of particle dielectrophoresis in two dimensions. The flow field and the motion of particles are solved with the DF/FD method, the electric field is solved with the sharp inter- face method, and the electrostatic force on the particles is computed using the Maxwell stress tensor method. The proposed method is validated via three problems： effective conductivity of particle compos- ite between two planar plates, cell trapping in a channel, and motion of particles due to both conventional and traveling wave dielectrophoretic forces.
