In thermoelastic martensitic transformation, it is well established that the first martensite plate appearing upon cooling becomes the final one during reverse transformation to austenite upon heating. The results obt...In thermoelastic martensitic transformation, it is well established that the first martensite plate appearing upon cooling becomes the final one during reverse transformation to austenite upon heating. The results obtained from this work show that the transformation sequence of the martensite appears to be random. Newly formed martensite plates can modify the elastic strain energy level stored in the already existing martensite. Additionally, the elastic strain energy stored in newly formed martensite is not necessarily to be higher than the remaining martensite. The obtained results may assist in understanding phenomena related to partial transformation of shape memory alloys, such as temperature memory effect.展开更多
In this work, the in situ optical observation was carried out in complete and incomplete transformation cycles of Ni–Ti alloys. In complete transformation cycles, initial martensite plates nucleate randomly in austen...In this work, the in situ optical observation was carried out in complete and incomplete transformation cycles of Ni–Ti alloys. In complete transformation cycles, initial martensite plates nucleate randomly in austenite. However, in a partial transformation cycle, the existing martensite plates have an influence on guiding the formation of subsequent martensite plates. And the randomness decreases with the decrease in transformation volume involved in the partial cycle. It is suggested that the randomness of transformations contributes to the introduction of defects, and the irreversibility associates with transformation randomness of martensite plates. For instance, a higher randomness in transformations could introduce more defects and more obvious irreversibility. On the other hand, defects generated in thermoelastic martensitic transformation are responsible for the hysteresis of transformations. Therefore, the randomness of transformations also contributes to the transformation hysteresis. These results could help further understanding on some martensitic transformation phenomena of shape memory alloys, such as the nonlinear and history-dependent characteristic.展开更多
Objective:Chronic kidney disease(CKD)is a complex disorder resulting from a combination of various environmental and genetic factors.Considerable efforts have been dedicated to elucidating its etiological mechanisms.N...Objective:Chronic kidney disease(CKD)is a complex disorder resulting from a combination of various environmental and genetic factors.Considerable efforts have been dedicated to elucidating its etiological mechanisms.Nevertheless,the pathogenic mechanism of CKD remains poorly understood,which hinders the development of effective therapeutic strategies.In this study,we aimed to identify novel mediators that could contribute to the development of CKD.Methods:The ClinVar,STRING,MEME Suite,TRRUST,bedtools,GEO,and R Studio databases and software were used to analyze their common features and investigate potential CKD disease genes.Transcriptomic analysis,immunohistochemistry,qRT-PCR,and Western blotting were utilized to further validate the role of ECT2 in kidney fibrosis.Results:In total,26 CKD disease genes were obtained from the ClinVar database,and the STRING,MEME Suite,TRRUST,bedtools,and GEO databases and software were used to analyze their common properties and explore potential CKD disease genes.Epithelial cell transforming sequence 2(ECT2),cyclin B 1,caspase 7 and collagen alpha-1(Ⅳ)were identified as potential candidates for CKD progression.Weighted correlation network analysis(WGCNA)subsequently revealed the relationships between potential genes and CKD.The results of the transcriptomic analysis further confirmed that ECT2 expression was greater in the kidney tissue of CKD patients than in that of healthy controls.Next,immunohistochemistry and Western blotting demonstrated that ECT2 was predominantly expressed in the renal tubules of a unilateral ureteral obstruction(UUO)mouse model.Consistently,in vitro experiments revealed that ECT2 was upregulated in TGF-β1-treated HK-2 cells.Moreover,ECT2 overexpression or knockdown in HK-2 cells altered the intensity of fibrosis markers.Conclusion:ECT2 significantly affects the development and progression of CKD,particularly in association with tubulointerstitial fibrosis.展开更多
BACKGROUND: Pancreatic cancer is closely related to epigenetic abnormality. The epithelial cell transforming sequence 2 gene (ECT2) plays a critical role in Rho activation during cytokinesis, and thus may play a role ...BACKGROUND: Pancreatic cancer is closely related to epigenetic abnormality. The epithelial cell transforming sequence 2 gene (ECT2) plays a critical role in Rho activation during cytokinesis, and thus may play a role in the pathogenesis of pancreatic cancer. In this study, we investigated the relationships between aberrant expression and epigenetic changes of the ECT2 gene in pancreatic cancer. METHODS: Four cell lines (PANC-1, Colo357, T3M-4 and PancTu I) and pancreatic ductal adenocarcinoma (PDAC) tissues were used for mRNA detection. After restriction isoschizomer endonucleases (Msp I/Hpa II) were used to digest the DNA sequence (5'-CCGG-3'), PCR was made to amplify the product. And RT-PCR was applied to determine the expression of the gene. RESULTS: The mRNA expression of the ECT2 gene was higher in pancreatic tumor tissue than in normal tissue. The gene was also expressed in the 4 PDAC cell lines. The methylation states of the upstream regions of the ECT2 gene were almost identical in normal, tumor pancreatic tissues, and the 4 PDAC cell lines. Some of the 5'-CCGG-3' areas in the upstream region of ECT2 were methylated, while others were unmethylated. CONCLUSIONS: The oncogene ECT2 is overexpressed in pancreatic tumor tissues as verified by RT-PCR detection. The methylation status of DNA in promoter areas is involved in the gene expression, along with other factors, in pancreatic cancer.展开更多
Speedometer identification has been researched for many years.The common approaches to that problem are usually based on image subtraction,which does not adapt to image offsets caused by camera vibration.To cope with ...Speedometer identification has been researched for many years.The common approaches to that problem are usually based on image subtraction,which does not adapt to image offsets caused by camera vibration.To cope with the rapidity,robust and accurate requirements of this kind of work in dynamic scene,a fast speedometer identification algorithm is proposed,it utilizes phase correlation method based on regional entire template translation to estimate the offset between images.In order to effectively reduce unnecessary computation and false detection rate,an improved linear Hough transform method with two optimization strategies is presented for pointer line detection.Based on VC＋＋ 6.0 software platform with OpenCV library,the algorithm performance under experiments has shown that it celerity and precision.展开更多
基金supported by Science Foundation of China University of Petroleum,Beijing(No.KYJJ2012-06-25)
文摘In thermoelastic martensitic transformation, it is well established that the first martensite plate appearing upon cooling becomes the final one during reverse transformation to austenite upon heating. The results obtained from this work show that the transformation sequence of the martensite appears to be random. Newly formed martensite plates can modify the elastic strain energy level stored in the already existing martensite. Additionally, the elastic strain energy stored in newly formed martensite is not necessarily to be higher than the remaining martensite. The obtained results may assist in understanding phenomena related to partial transformation of shape memory alloys, such as temperature memory effect.
基金financially supported by the Science Foundation of China University of PetroleumBeijing(No.KYJJ2012-06-25)
文摘In this work, the in situ optical observation was carried out in complete and incomplete transformation cycles of Ni–Ti alloys. In complete transformation cycles, initial martensite plates nucleate randomly in austenite. However, in a partial transformation cycle, the existing martensite plates have an influence on guiding the formation of subsequent martensite plates. And the randomness decreases with the decrease in transformation volume involved in the partial cycle. It is suggested that the randomness of transformations contributes to the introduction of defects, and the irreversibility associates with transformation randomness of martensite plates. For instance, a higher randomness in transformations could introduce more defects and more obvious irreversibility. On the other hand, defects generated in thermoelastic martensitic transformation are responsible for the hysteresis of transformations. Therefore, the randomness of transformations also contributes to the transformation hysteresis. These results could help further understanding on some martensitic transformation phenomena of shape memory alloys, such as the nonlinear and history-dependent characteristic.
基金Supported by Natural Science Foundations of China (11101108, 11171301, 10771191 and 10471124)Natural Science Foundation of Zhejiang Province of China (Y6090105)
文摘Let X and Y be Banach spaces, 0 〈 q 〈 ＋∞, i ≤ p 〈 ＋∞. In this paper, we characterize matrix transformations of lq （ X ） to lp （ Y ）.
基金supported by grants from the Fundamental Research Funds for the Central Universities(No.YCJJ202201036)National Natural Science Foundation of China(No.81873602 and No.82170773).
文摘Objective:Chronic kidney disease(CKD)is a complex disorder resulting from a combination of various environmental and genetic factors.Considerable efforts have been dedicated to elucidating its etiological mechanisms.Nevertheless,the pathogenic mechanism of CKD remains poorly understood,which hinders the development of effective therapeutic strategies.In this study,we aimed to identify novel mediators that could contribute to the development of CKD.Methods:The ClinVar,STRING,MEME Suite,TRRUST,bedtools,GEO,and R Studio databases and software were used to analyze their common features and investigate potential CKD disease genes.Transcriptomic analysis,immunohistochemistry,qRT-PCR,and Western blotting were utilized to further validate the role of ECT2 in kidney fibrosis.Results:In total,26 CKD disease genes were obtained from the ClinVar database,and the STRING,MEME Suite,TRRUST,bedtools,and GEO databases and software were used to analyze their common properties and explore potential CKD disease genes.Epithelial cell transforming sequence 2(ECT2),cyclin B 1,caspase 7 and collagen alpha-1(Ⅳ)were identified as potential candidates for CKD progression.Weighted correlation network analysis(WGCNA)subsequently revealed the relationships between potential genes and CKD.The results of the transcriptomic analysis further confirmed that ECT2 expression was greater in the kidney tissue of CKD patients than in that of healthy controls.Next,immunohistochemistry and Western blotting demonstrated that ECT2 was predominantly expressed in the renal tubules of a unilateral ureteral obstruction(UUO)mouse model.Consistently,in vitro experiments revealed that ECT2 was upregulated in TGF-β1-treated HK-2 cells.Moreover,ECT2 overexpression or knockdown in HK-2 cells altered the intensity of fibrosis markers.Conclusion:ECT2 significantly affects the development and progression of CKD,particularly in association with tubulointerstitial fibrosis.
文摘BACKGROUND: Pancreatic cancer is closely related to epigenetic abnormality. The epithelial cell transforming sequence 2 gene (ECT2) plays a critical role in Rho activation during cytokinesis, and thus may play a role in the pathogenesis of pancreatic cancer. In this study, we investigated the relationships between aberrant expression and epigenetic changes of the ECT2 gene in pancreatic cancer. METHODS: Four cell lines (PANC-1, Colo357, T3M-4 and PancTu I) and pancreatic ductal adenocarcinoma (PDAC) tissues were used for mRNA detection. After restriction isoschizomer endonucleases (Msp I/Hpa II) were used to digest the DNA sequence (5'-CCGG-3'), PCR was made to amplify the product. And RT-PCR was applied to determine the expression of the gene. RESULTS: The mRNA expression of the ECT2 gene was higher in pancreatic tumor tissue than in normal tissue. The gene was also expressed in the 4 PDAC cell lines. The methylation states of the upstream regions of the ECT2 gene were almost identical in normal, tumor pancreatic tissues, and the 4 PDAC cell lines. Some of the 5'-CCGG-3' areas in the upstream region of ECT2 were methylated, while others were unmethylated. CONCLUSIONS: The oncogene ECT2 is overexpressed in pancreatic tumor tissues as verified by RT-PCR detection. The methylation status of DNA in promoter areas is involved in the gene expression, along with other factors, in pancreatic cancer.
基金Supported by the National Natural Science Foundation of China (61004139)Beijing Municipal Natural Science Foundation(4101001)2008 Yangtze Fund Scholar and Innovative Research Team Development Schemes of Ministry of Education
文摘Speedometer identification has been researched for many years.The common approaches to that problem are usually based on image subtraction,which does not adapt to image offsets caused by camera vibration.To cope with the rapidity,robust and accurate requirements of this kind of work in dynamic scene,a fast speedometer identification algorithm is proposed,it utilizes phase correlation method based on regional entire template translation to estimate the offset between images.In order to effectively reduce unnecessary computation and false detection rate,an improved linear Hough transform method with two optimization strategies is presented for pointer line detection.Based on VC＋＋ 6.0 software platform with OpenCV library,the algorithm performance under experiments has shown that it celerity and precision.
