Mahalanobis-Taguchi system(MTS)is a kind of data mining and pattern recognition method which can identify the attribute characteristics of multidimensional data by constructing Mahalanobis distance(MD)measurement scal...Mahalanobis-Taguchi system(MTS)is a kind of data mining and pattern recognition method which can identify the attribute characteristics of multidimensional data by constructing Mahalanobis distance(MD)measurement scale.In this paper,considering the influence of irregular distribution of the sample data and abnormal variation of the normal data on accuracy of MTS,a feature recognition and selection model of the equipment state based on the improved MTS is proposed,and two aspects of the model namely construction of the original Mahalanobis space(MS)and determination of the threshold are studied.Firstly,the original training sample space is statistically controlled by the X-bar-S control chart,and extreme data of the single characteristic attribute is filtered to reduce the impact of extreme condition on the accuracy of the model,so as to construct a more robust MS.Furthermore,the box plot method is used to determine the threshold of the model.And the stability of the model and the tolerance to the extreme condition are improved by leaving sufficient range of the variation for the extreme condition which is identified as in the normal range.Finally,the improved model is compared with the traditional one based on the unimproved MTS by using the data from the literature.The result shows that compared with the traditional model,the accuracy and sensitivity of the improved model for state identification can be greatly enhanced.展开更多
Validating a method of analysis goes through different steps, which aims at testing the normality of measurements distribution, estimating the uncertainty of the components of a measurement （i.e., accuracy and correc...Validating a method of analysis goes through different steps, which aims at testing the normality of measurements distribution, estimating the uncertainty of the components of a measurement （i.e., accuracy and correctness）, and finally, define the control tests of non degradation of the method performances. This paper outlines the steps for validating a biological method of analysis. It involves the construction of an experimental design, a statistical model, and the preparation of an interne laboratory reference material （pilot vaccine）. The latter is used to study the impact of deviation and variation factors, in order to, optimize the analytical method, to evaluate the bias （random error）, and to calculate the uncertainty of measurement, and make the control charts. This method is applied in the titration of live viral vaccines of Gumboro disease on chicken＇s embryos fibroblasts. The experimental results show that potential influence factors related to the titration method had no significant influence on the obtained results. Taking into account these results, an operating mode has been elaborated. The finalized method proved to be faithful to standard deviation of repeatability and reproducibility of 0.21 and 0.22, respectively, with a confidence level of 95%. The calculated uncertainty of measurement is equal to 0.2, which represents the average error level of a titer. A homogeneous stock of interne laboratory reference vaccine （MRIL）, with an average titer of 5.9 log DIT 50, was produced and the control chart set in away to provide the laboratory with an important tool of control and monitoring of the viral titers evolution in time, as well as, the mastery of the validated titration method performances.展开更多
基金the National Natural Science Foundation of China(No.71401016)the Shaanxi Provincial Natural Science Foundation of China(No.2019JM-495)the Fundamental Research Funds for Central Universities of Chang'an University(Nos.300102228110 and 300102228402)。
文摘Mahalanobis-Taguchi system(MTS)is a kind of data mining and pattern recognition method which can identify the attribute characteristics of multidimensional data by constructing Mahalanobis distance(MD)measurement scale.In this paper,considering the influence of irregular distribution of the sample data and abnormal variation of the normal data on accuracy of MTS,a feature recognition and selection model of the equipment state based on the improved MTS is proposed,and two aspects of the model namely construction of the original Mahalanobis space(MS)and determination of the threshold are studied.Firstly,the original training sample space is statistically controlled by the X-bar-S control chart,and extreme data of the single characteristic attribute is filtered to reduce the impact of extreme condition on the accuracy of the model,so as to construct a more robust MS.Furthermore,the box plot method is used to determine the threshold of the model.And the stability of the model and the tolerance to the extreme condition are improved by leaving sufficient range of the variation for the extreme condition which is identified as in the normal range.Finally,the improved model is compared with the traditional one based on the unimproved MTS by using the data from the literature.The result shows that compared with the traditional model,the accuracy and sensitivity of the improved model for state identification can be greatly enhanced.
文摘Validating a method of analysis goes through different steps, which aims at testing the normality of measurements distribution, estimating the uncertainty of the components of a measurement （i.e., accuracy and correctness）, and finally, define the control tests of non degradation of the method performances. This paper outlines the steps for validating a biological method of analysis. It involves the construction of an experimental design, a statistical model, and the preparation of an interne laboratory reference material （pilot vaccine）. The latter is used to study the impact of deviation and variation factors, in order to, optimize the analytical method, to evaluate the bias （random error）, and to calculate the uncertainty of measurement, and make the control charts. This method is applied in the titration of live viral vaccines of Gumboro disease on chicken＇s embryos fibroblasts. The experimental results show that potential influence factors related to the titration method had no significant influence on the obtained results. Taking into account these results, an operating mode has been elaborated. The finalized method proved to be faithful to standard deviation of repeatability and reproducibility of 0.21 and 0.22, respectively, with a confidence level of 95%. The calculated uncertainty of measurement is equal to 0.2, which represents the average error level of a titer. A homogeneous stock of interne laboratory reference vaccine （MRIL）, with an average titer of 5.9 log DIT 50, was produced and the control chart set in away to provide the laboratory with an important tool of control and monitoring of the viral titers evolution in time, as well as, the mastery of the validated titration method performances.
