Due to its technological advantages,single-cell sequencing has become an increasingly utilized tool to unravel the heterogeneity and complexity of individuals.The preparation of a high-quality single-cell suspension f...Due to its technological advantages,single-cell sequencing has become an increasingly utilized tool to unravel the heterogeneity and complexity of individuals.The preparation of a high-quality single-cell suspension from solid tissues is a critical step prior to single-cell RNA sequencing.Based on a brief overview of the steps involved in preparing cell suspensions from solid tissues for single-cell RNA sequencing,we present a detailed analysis of the pivotal steps in the preparation process,including enzymatic digestion,mechanical dissociation,and cell viability evaluation.The clarification of these experimental details will greatly help us obtain high-quality single-cell sequencing data from solid tissues.展开更多
The production of plastics has exponentially increased over time such that the observation of microplastics in the oceans has become a major concern given how frequently these particles and marine biota may interact.M...The production of plastics has exponentially increased over time such that the observation of microplastics in the oceans has become a major concern given how frequently these particles and marine biota may interact.Microplastics can be ingested by diverse species,including invertebrates,which can lead to physical damage due to their small size and potentially chemical damage related to the ingestion of monomers,polymeric additives,or adsorbed chemicals.This study reports on the first evidence of microplastics in Mnemiopsis leidyi.Microplastics were described by type,quantified,and had their chemical composition identified.In total,328 samples of microplastic(MP)candidates were observed:blue fibers were the most abundant,followed by black,red,and green fibers as well as blue fragments.Three types of microplastics were chemically identified as polyurethane,unplasticized polyvinylchloride,and polyethylene polylauryllactam.Overall,this study demonstrated that Mnemiopsis leidyi have the capacity to incorporate microplastics.Although more studies are needed,Mnemiopsis leidyi is relatively abundant on the Atlantic coast and should be recommended for use as a bioindicator for microplastics.展开更多
基金supported by the National Key R&D Program of China(2023YFC2308300,2023YFC2306800).
文摘Due to its technological advantages,single-cell sequencing has become an increasingly utilized tool to unravel the heterogeneity and complexity of individuals.The preparation of a high-quality single-cell suspension from solid tissues is a critical step prior to single-cell RNA sequencing.Based on a brief overview of the steps involved in preparing cell suspensions from solid tissues for single-cell RNA sequencing,we present a detailed analysis of the pivotal steps in the preparation process,including enzymatic digestion,mechanical dissociation,and cell viability evaluation.The clarification of these experimental details will greatly help us obtain high-quality single-cell sequencing data from solid tissues.
基金supported by FAPES/VALE/FAPERJ[grant number 01/2015].
文摘The production of plastics has exponentially increased over time such that the observation of microplastics in the oceans has become a major concern given how frequently these particles and marine biota may interact.Microplastics can be ingested by diverse species,including invertebrates,which can lead to physical damage due to their small size and potentially chemical damage related to the ingestion of monomers,polymeric additives,or adsorbed chemicals.This study reports on the first evidence of microplastics in Mnemiopsis leidyi.Microplastics were described by type,quantified,and had their chemical composition identified.In total,328 samples of microplastic(MP)candidates were observed:blue fibers were the most abundant,followed by black,red,and green fibers as well as blue fragments.Three types of microplastics were chemically identified as polyurethane,unplasticized polyvinylchloride,and polyethylene polylauryllactam.Overall,this study demonstrated that Mnemiopsis leidyi have the capacity to incorporate microplastics.Although more studies are needed,Mnemiopsis leidyi is relatively abundant on the Atlantic coast and should be recommended for use as a bioindicator for microplastics.
