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A Pragmatic Approach to Product Costing Based on Standard Time Estimation
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作者 JIAO Yuan-yuan, HAN Wen-xiu, DU JunSchool of Management, Tianjin University, Tianjin 300072, China 《Journal of Systems Science and Systems Engineering》 SCIE EI CSCD 2001年第2期197-202,共6页
The major drawbacks of traditional approaches to product costing include lack of manufacturing knowledge, reliance on the detailed design description, poor cost function approximation and inability to update estimatio... The major drawbacks of traditional approaches to product costing include lack of manufacturing knowledge, reliance on the detailed design description, poor cost function approximation and inability to update estimation algorithms by using actual cost data. Adopting the Activity-Based Costing (ABC) concept and based on estimated processing time, this paper proposes a pragmatic approach to product costing. The approach involves two stages, namely the preparatory stage and the estimation stage. A case study conducted in a refrigerator company is reported. 展开更多
关键词 product costing activity-based costing standard time
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The time and frequency standard system for FAST receivers 被引量:2
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作者 Kai Zhu Jin Fan +16 位作者 Kun Liang Wei Tang Zhi-Sheng Gao Yan Zhu Yi Feng Wei-Wei Zhu Lei Qian You-Ling Yue Jin-You Song Xiang-Wei Shi Xing-Yi Wang Ming-Lei Guo Hang Zhang Heng-Qian Gan Hong-Fei Liu Cheng-Jin Jin Peng Jiang 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2020年第5期148-154,共7页
This paper reports on the time and frequency standard system for the Five-hundred meter Aperture Spherical radio Telescope(FAST),including the system design,stability measurements and pulsar timing observations.The st... This paper reports on the time and frequency standard system for the Five-hundred meter Aperture Spherical radio Telescope(FAST),including the system design,stability measurements and pulsar timing observations.The stability and drift rate of the frequency standard are calculated using 1-year monitoring data.The UTC-NIM Disciplined Oscillator(NIMDO)system improves the system time accuracy and stability to the level of 5 ns.Pulsar timing observations were carried out for several months.The weighted RMS of timing residuals reaches the level of less than 3.0μs. 展开更多
关键词 FAST:radio telescope time frequency standard NIMDO:UTC-NIM
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Noether symmetry and conserved quantity for dynamical system with non-standard Lagrangians on time scales 被引量:12
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作者 宋静 张毅 《Chinese Physics B》 SCIE EI CAS CSCD 2017年第8期201-209,共9页
This paper focuses on studying the Noether symmetry and the conserved quantity with non-standard Lagrangians, namely exponential Lagrangians and power-law Lagrangians on time scales. Firstly, for each case, the Hamilt... This paper focuses on studying the Noether symmetry and the conserved quantity with non-standard Lagrangians, namely exponential Lagrangians and power-law Lagrangians on time scales. Firstly, for each case, the Hamilton prin- ciple based on the action with non-standard Lagrangians on time scales is established, with which the corresponding Euler-Lagrange equation is given. Secondly, according to the invariance of the Hamilton action under the infinitesimal transformation, the Noether theorem for the dynamical system with non-standard Lagrangians on time scales is established. The proof of the theorem consists of two steps. First, it is proved under the infinitesimal transformations of a special one-parameter group without transforming time. Second, utilizing the technique of time-re-parameterization, the Noether theorem in a general form is obtained. The Noether-type conserved quantities with non-standard Lagrangians in both clas- sical and discrete cases are given. Finally, an example in Friedmann-Robertson-Walker spacetime and an example about second order Duffing equation are given to illustrate the application of the results. 展开更多
关键词 time scale non-standard Lagrangian Noether symmetry conserved quantity
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Estimation of Standard Operation Time of Flight Legs Based on Clustering and Probability Analysis
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作者 Yuan Ligang Hu Minghua +1 位作者 Xie Hua Li Yinfeng 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI CSCD 2016年第4期491-501,共11页
A clustering algorithm and a probability statistics method were applied to different phases of a flight to analyze operation time during aircraft ground taxiing and airborne flight.And the clustering pattern,distribut... A clustering algorithm and a probability statistics method were applied to different phases of a flight to analyze operation time during aircraft ground taxiing and airborne flight.And the clustering pattern,distribution characteristics and dynamically changing rules of the two phases were identified.Further,an estimate method was established to measure operation time of flight legs,with creative steps of calculating individual segment separately and then integrating them accordingly.The method can both objectively and dynamically measure operation time,and accurately reflect real situation.It helps to better utilize airport slot resources and provides a strong support for air traffic flow management when scheduling flight plan in strategic and pre-tactic phases. 展开更多
关键词 flight leg standard operation time CLUSTERING probability analysis
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Generation and Application of A Standardized Load-Time History to Tubular T-joints in Offshore Platforms 被引量:1
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作者 李珊珊 崔维成 《China Ocean Engineering》 SCIE EI CSCD 2015年第5期633-648,共16页
Marine structures are mostly made of metals and always experience complex random loading during their service periods. The fatigue crack growth behaviors of metal materials have been proved from laboratory tests to be... Marine structures are mostly made of metals and always experience complex random loading during their service periods. The fatigue crack growth behaviors of metal materials have been proved from laboratory tests to be sensitive to the loading sequence encountered. In order to take account of the loading sequence effect, fatigue life prediction should be based on fatigue crack propagation(FCP) theory rather than the currently used cumulative fatigue damage(CFD) theory. A unified fatigue life prediction(UFLP) method for marine structures has been proposed by the authors' group. In order to apply the UFLP method for newly designed structures, authorities such as the classification societies should provide a standardized load-time history(SLH) such as the TWIST and FALSTAFF sequences for transport and fighter aircraft. This paper mainly aims at proposing a procedure to generate the SLHs for marine structures based on a short-term loading sample and to provide an illustration on how to use the presented SLH to a typical tubular T-joint in an offshore platform based on the UFLP method. 展开更多
关键词 standardized load-time history(SLH) unified fatigue life prediction(UFLP) method fatigue crack propagation(FCP) theory tubular T-joint
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An improved formula for standard hypoxia tolerance time to evaluate hypoxic tolerance in mice
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作者 Gang Xu Yu-Qi Gao +3 位作者 Yi-Xing Gao Gang Wu Jian-Yang Zhang Wen-Xiang Gao 《Military Medical Research》 SCIE CAS CSCD 2019年第2期111-116,共6页
Background: Hypoxia is a primary cause of mountain sickness and a common pathological condition in patients with heart failure, shock, stroke, and chronic obstructive pulmonary disease(COPD). Thus far, little advancem... Background: Hypoxia is a primary cause of mountain sickness and a common pathological condition in patients with heart failure, shock, stroke, and chronic obstructive pulmonary disease(COPD). Thus far, little advancement in countering hypoxic damage has been achieved, and one of the main reasons is the absence of an ideal algorithm or calculation method to normalize hypoxia tolerance scores when evaluating an animal model. In this study, we improved a traditional calculation formula for assessment of hypoxia tolerance.Methods: We used a sealed bottle model in which the oxygen is gradually consumed by a mouse inside. To evaluate the hypoxia tolerance of mice, the survival time(ST) of the mouse is recorded and was used to calculate standard hypoxia tolerance time(STT) and adjusted standard hypoxia tolerance time(ASTT). Mice administered with methazolamide and saline were used as positive and negative controls, respectively.Results: Since mice were grouped according to either body weight(BW) or bottle volume, we found a strongly negative correlation between STT and BW instead of between STT and bottle volume, suggesting that different BWs could cause false positive or negative errors in the STT results. Furthermore, both false positive and negative errors could be rectified when ASTT was used as the evaluation index. Screening for anti-hypoxic medicines by using mice as the experimental subjects would provide more credible results with the improved ASTT method than with the STT method.Conclusions: ASTT could be a better index than STT for the evaluation of hypoxia tolerance abilities as it could eliminate the impact of animal BW. 展开更多
关键词 HYPOXIA standard HYPOXIC TOLERANCE time MICE model Body weight
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Investigating the Use of <i>Jatropha</i>Biodiesel in Compression Ignition Engines by Comparing Effects of Storage Time on Its Properties with the Standard Properties of Fossil-Diesel and Properties of Quality Biodiesel
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作者 Gershom Mwandila 《Energy and Power Engineering》 2012年第5期349-352,共4页
Effects of storage time on some properties of jatropha biodiesel were investigated over the storage time of 0 to 8 weeks at the temperature of 25℃. Such properties as water content and density were found to increase ... Effects of storage time on some properties of jatropha biodiesel were investigated over the storage time of 0 to 8 weeks at the temperature of 25℃. Such properties as water content and density were found to increase at the rates of 5 ppm and 14 kg/m3 per week respectively. These rates translate into property values which compare closely with the standard properties of fossil-diesel and properties of quality biodiesel. As a result, the jatropha biodiesel can be used as alternative fuel to fossil-diesel in compression ignition engines within 8 weeks of its production. However, the calorific value and flash point of the biodiesel decreased at the rate of 1.4℃ and 2.5 MJ/kg per week respectively. Although the flash point was within the acceptable level for quality biodiesel, the calorific values were abnormally higher than the values for quality biodiesel of about 38.7 MJ/kg. With the exception of the calorific values, the other properties were not reasonably affected. 展开更多
关键词 PROPERTIES Fossil-Diesel QUALITY BIODIESEL standard PROPERTIES Storage time
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China National Standards on Nano-Materials Issued for the First Time
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《China Standardization》 2005年第2期4-5,共2页
On February 28th, 2005, AQSIQ (the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China), and SAC (the Standardization Administration of P. R. China)jointly ho... On February 28th, 2005, AQSIQ (the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China), and SAC (the Standardization Administration of P. R. China)jointly hosted the press conference on seven items of national standards on nano-materials in Beijing. The seven items of national standards were approved on Sept. 29th and Dec. 27th, 2004 respectively by SAC, and publicized in Jan. 2005. They will be formally put into practice from April 1st, 2005. 展开更多
关键词 NANO China National standards on Nano-Materials Issued for the First time
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基于OSEKTime的共存模型的改进与实现 被引量:3
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作者 谢昊飞 陈家佳 舒强 《重庆邮电大学学报(自然科学版)》 北大核心 2009年第5期658-661,共4页
OSEKTime规范提出了基于时间触发操作系统与基于OSEK/VDX标准实现的操作系统的共存模型。在分析OSEKTime规范的基础上,针对该规范的消息触发任务存在着非实时性问题,提出并实现了一种合理的、能与OS-EK OS共存的实时时间触发操作系统,... OSEKTime规范提出了基于时间触发操作系统与基于OSEK/VDX标准实现的操作系统的共存模型。在分析OSEKTime规范的基础上,针对该规范的消息触发任务存在着非实时性问题,提出并实现了一种合理的、能与OS-EK OS共存的实时时间触发操作系统,确保了时间触发任务的实时性和对消息触发任务的死限时间内的及时处理。 展开更多
关键词 OSEK/VDX标准 OSEKtime规范 时间触发 消息触发
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Real-time PCR检测风疹病毒方法学建立及临床应用 被引量:2
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作者 蒲小蓉 张德纯 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2007年第6期1352-1356,共5页
为了建立一种能广泛应用于临床快速、简便、灵敏度和特异性较好的风疹病毒(RV)定量诊断方法。针对RV基因保守序列设计两对引物和一条荧光双标记探针。将PCR扩增所得到的产物片段克隆,作为定量检测的标准品,进行Real—timePCR检测,... 为了建立一种能广泛应用于临床快速、简便、灵敏度和特异性较好的风疹病毒(RV)定量诊断方法。针对RV基因保守序列设计两对引物和一条荧光双标记探针。将PCR扩增所得到的产物片段克隆,作为定量检测的标准品,进行Real—timePCR检测,绘制标准曲线。将此方法和ELISA试剂盒平行检测50份孕妇血清,评估两种方法检出阳性率的差异显著性。Real—timePCR法能较好地检出RVcDNA载量。曲线的相关系数(r)为0.998,可检测线性范围大约在10^3~10^9copies/μl,灵敏度接近10^3copies/μl;批间、批内CV值分别为3.36%、0.94%;也有较好的特异性。与经典的ELISA方法相比,有显著性差异。Real—timePCR方法操作简单、快速,并能避免PCR后处理导致的假阳性污染,实现实时定量。此方法对RV感染的临床诊断和疗效判断等方面有较大的指导意义。 展开更多
关键词 风疹病毒 实时荧光定量PCR 标准曲线
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A Computer Vision-Based Model for Automatic Motion Time Study
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作者 Jirasak Ji Warut Pannakkong Jirachai Buddhakulsomsiri 《Computers, Materials & Continua》 SCIE EI 2022年第11期3557-3574,共18页
Motion time study is employed by manufacturing industries to determine operation time.An accurate estimate of operation time is crucial for effective process improvement and production planning.Traditional motion time... Motion time study is employed by manufacturing industries to determine operation time.An accurate estimate of operation time is crucial for effective process improvement and production planning.Traditional motion time study is conducted by human analysts with stopwatches,which may be exposed to human errors.In this paper,an automated time study model based on computer vision is proposed.The model integrates a convolutional neural network,which analyzes a video of a manual operation to classify work elements in each video frame,with a time study model that automatically estimates the work element times.An experiment is conducted using a grayscale video and a color video of a manual assembly operation.The work element times from the model are statistically compared to the reference work element time values.The result shows no statistical difference among the time data,which clearly demonstrates the effectiveness of the proposed model. 展开更多
关键词 Motion time study computer vision convolutional neural network manual operation standard time
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金黄色葡萄球菌基因表达的DNA扣除法Real-time RT PCR相对定量分析 被引量:4
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作者 相丽 姜毓君 +3 位作者 刘伟 毕宇涵 赵凤 霍贵成 《微生物学报》 CAS CSCD 北大核心 2008年第4期526-531,共6页
【目的】金黄色葡萄球菌作为一种分布广泛的致病微生物和研究革兰氏阳性菌遗传背景的模式菌株,利用real-time RT PCR对相关毒素及调控基因进行表达定量分析,在生物、医学、食品检测等领域具有较大研究价值。【方法】对制备好的反转录(RT... 【目的】金黄色葡萄球菌作为一种分布广泛的致病微生物和研究革兰氏阳性菌遗传背景的模式菌株,利用real-time RT PCR对相关毒素及调控基因进行表达定量分析,在生物、医学、食品检测等领域具有较大研究价值。【方法】对制备好的反转录(RT,含有cDNA和DNA)和非反转录(RT—,仅含DNA)样品进行Real-time PCR检测,根据经典(1+E)—△△Ct相对定量算法并结合PCR效率公式建立一种基因表达相对定量分析的DNA扣除法,将得到的Ct值转换为各样品含量,从RT样品中扣除RT—样品的量,无需DNaseⅠ酶解处理就可以去除DNA的影响,RT—样品的检测结果还可同时作为稳定的DNA内参。【结果】采用以上方法分析金黄色葡萄球菌肠毒素A基因(sea)、16S rRNA和RNAⅢ的表达情况,在含有葡萄糖的NB培养基中sea的相对转录水平随着葡萄糖浓度的增大而升高,RNAⅢ的相对转录水平随葡萄糖浓度的变化而产生小幅度的波动,16S rRNA在菌体生长初期时的表达量较为稳定;与绝对定量法比较,结果差异较小(均小于15%),且差异不显著(p>0.05)。【结论】这种基于DNA扣除法的Real-time RT PCR相对定量方法可以有效的对金黄色葡萄球菌的基因表达进行分析。 展开更多
关键词 金黄色葡萄球菌 Real—time RT PCR 相对定量 内参 DNA扣除法
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升降机导轨架中标准节磨损程度的检测系统
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作者 陈晓峰 史宏旻 +2 位作者 周俊林 王海峰 衡铮 《建筑机械》 2026年第1期206-209,共4页
高层建筑施工过程中,施工升降机导轨架的安全性至关重要,直接影响施工人员和物料垂直运输效率与安全性。由于吊笼在沿导轨架往复运行时与标准节产生持续摩擦,标准节在长期服役中不可避免地出现磨损,当磨损程度达到失效阈值便会极大地增... 高层建筑施工过程中,施工升降机导轨架的安全性至关重要,直接影响施工人员和物料垂直运输效率与安全性。由于吊笼在沿导轨架往复运行时与标准节产生持续摩擦,标准节在长期服役中不可避免地出现磨损,当磨损程度达到失效阈值便会极大地增加施工事故风险。文章针对目前缺乏对标准节磨损程度的实时、准确评估手段的现状,在已有研究与专利技术基础上,提出了1种以标准节通过次数与速度影响参数为核心的检测系统。该系统通过在每个标准节上贴附具有唯一ID的标签,再结合装设于吊笼上的检测装置与速度传感器,实时监测吊笼对各标准节的实际经过次数及实际运行速度,与预存的失效总次数相比较,利用速度影响因子对磨损程度做动态修正,从而实现对标准节磨损程度的在线监测及预警。试验结果与应用示范表明,该系统具有部署成本低、检测精度高、实时性和可扩展性良好的优点,可有效保障升降机在高层建筑施工中的安全与效率。 展开更多
关键词 升降机 导轨架 标准节 磨损检测 速度影响 实时监测
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低丰度基因Real-time PCR相对定量方法的建立
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作者 黄聪琳 王晓琳 +1 位作者 郭敏 姜华 《药物生物技术》 CAS 2014年第6期534-537,共4页
低丰度基因通常难以通过标准曲线法进行Real-time PCR。通过使用特异引物,对拟南芥低丰度表达基因CIPK7进行了转录水平上的定量分析,成功建立了可检测低丰度基因表达变化的Real-time PCR标准曲线定量方法。扩增CIPK7基因CDS全长,并对PC... 低丰度基因通常难以通过标准曲线法进行Real-time PCR。通过使用特异引物,对拟南芥低丰度表达基因CIPK7进行了转录水平上的定量分析,成功建立了可检测低丰度基因表达变化的Real-time PCR标准曲线定量方法。扩增CIPK7基因CDS全长,并对PCR产物进行纯化,使用纯化的PCR产物作为标准品,绘制标准曲线,对CIPK7基因的表达进行Real-time PCR分析。与常规Real-time PCR方法进行比较,结果表明,该方法得到的标准曲线,浓度区间范围广,线性关系好,能够很好地适用于低丰度基因的Real-time PCR标准曲线法定量。实验测得低温胁迫下拟南芥CIPK7基因的表达量,随低温处理时间增长,有明显的先升高后降低的变化。 展开更多
关键词 实时定量荧光PCR 低丰度表达基因 低温胁迫 基因表达 标准曲线 相对定量
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Spatio-Temporal Characteristics of Standardized Precipitation Index in the Taihu Basin during 1951-2000
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作者 LIU Xingcai XU Zongxue LIU Bo 《Wuhan University Journal of Natural Sciences》 CAS 2009年第6期518-524,共7页
Spatial and temporal characteristics of standardized precipitation index (SPI), which is widely used for drought/flood monitoring, are investigated in this study. The purpose is to obtain a reasonable primary scheme... Spatial and temporal characteristics of standardized precipitation index (SPI), which is widely used for drought/flood monitoring, are investigated in this study. The purpose is to obtain a reasonable primary scheme of zoning on the basis of drought/ wetness conditions in the study area. Spatio-temporal distributions of SPI with the time scales of 3 months and 12 months are investigated with the datasets of precipitation in the Taihu basin during past decades (1951-2000). Results indicate that SPI series of 3 months show random fluctuation while that of 12 months behaves like I/f noise. SPI series of 3 months show little trend while that of 12 months show significant trend at several stations. Drought magnitude (DM) is also estimated on the basis of SPI values to assess drought condition. No trend is detected in DMs with time scales of both 3 months and 12 months. Spatial variability of DM is analyzed by mapping the DM with 12 months for extreme drought and wetness, and regional characteristics are analyzed for DM. 展开更多
关键词 spatio-temporal distribution Taihu basin standardized precipitation index time scale ZONING
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不同采收期‘威海金’苹果品质及耐贮性研究 被引量:3
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作者 魏雯雯 贾连文 +4 位作者 任紫烟 连欢 杨相政 姚延琴 张黎军 《中国果菜》 2025年第4期1-6,40,共7页
本研究以不同采收期‘威海金’苹果为研究对象,通过测定其淀粉指数、硬度、可溶性固形物含量、呼吸速率、乙烯释放速率等指标,同时利用GC-MS分析挥发性香气成分的变化,以确定不同贮运时间的‘威海金’苹果采收标准。结果表明,采收期I(... 本研究以不同采收期‘威海金’苹果为研究对象,通过测定其淀粉指数、硬度、可溶性固形物含量、呼吸速率、乙烯释放速率等指标,同时利用GC-MS分析挥发性香气成分的变化,以确定不同贮运时间的‘威海金’苹果采收标准。结果表明,采收期I(盛花后165 d)的‘威海金’苹果硬度高达7.50 kg/cm^(2),但可溶性固形物和挥发性香气含量较低,对低温敏感,-0.5℃贮藏120 d开始出现冻害现象;采收期Ⅲ(盛花后195 d)果实可溶性固形物含量15.0%,风味、香气浓郁,但硬度下降至6.8 kg/cm^(2),贮藏至120 d,硬度下降至5.6 kg/cm^(2),开始出现果肉变绵,组织衰老、褐变现象,此阶段采收的果实适宜鲜销或短期贮藏;采收期II(盛花后180 d)的果实硬度7.40 kg/cm^(2),可溶性固形物含量15.40%,可滴定酸含量0.18%,淀粉染色指数7.82,此阶段采收的果实适合中长期贮藏,贮藏时间180~240 d。 展开更多
关键词 ‘威海金’苹果 贮藏时间 采收期 品质 标准
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Including Space-Time in the Extended Group Cl3* of Relativistic Form-Invariance
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作者 Claude Daviau Jacques Bertrand 《Journal of Modern Physics》 CAS 2022年第8期1147-1156,共10页
The inclusion of space-time in the extended group of relativistic form-invariance, Cl<sub>3</sub>*</sup>, is specified as the inclusion of the whole space-time manifold in this multiplicative Lie gro... The inclusion of space-time in the extended group of relativistic form-invariance, Cl<sub>3</sub>*</sup>, is specified as the inclusion of the whole space-time manifold in this multiplicative Lie group. First physical results presented here are: the geometric origin of the time arrow, a better understanding of the non-simultaneity in optics and a mainly geometric origin for the universe expansion, and its recent acceleration. 展开更多
关键词 Space-time Manifold Invariance Group standard Model Acceleration of Expansion
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Three-dimensional Simulation of Reverberation Chamber Using Finite-element Time-domain Method 被引量:1
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作者 DU Lei WANG Song +2 位作者 CUI Yaozhong WANG Qingguo PAN Yun 《高电压技术》 EI CAS CSCD 北大核心 2013年第12期2889-2893,共5页
关键词 三维模拟 时域法 有限元 混响室 几何建模 标准偏差 雷达散射截面 低频响应
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我国新质生产力发展的区域差异、时空演进及驱动因素识别
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作者 周恩宇 赵浪 肖秋香 《上海对外经贸大学学报》 北大核心 2025年第5期41-58,共18页
评价我国新质生产力发展水平,探析区域差异与时空特征,并对驱动因素进行识别,对实现我国新质生产力因地制宜发展有着重要现实意义。本文基于2012~2022年中国30个省级面板数据,从生产力技术化、绿色化、数字化维度出发构建评价指标体系,... 评价我国新质生产力发展水平,探析区域差异与时空特征,并对驱动因素进行识别,对实现我国新质生产力因地制宜发展有着重要现实意义。本文基于2012~2022年中国30个省级面板数据,从生产力技术化、绿色化、数字化维度出发构建评价指标体系,并运用熵值法、Dagum基尼系数及分解法、标准差椭圆、地理探测器分析我国新质生产力发展水平的区域差异、时空演进及驱动因素。研究发现:(1)从发展水平来看,我国新质生产力总体发展水平呈缓慢上升趋势,区域发展上呈现出东部>中部>东北>西部的发展格局。(2)从区域差异来看,我国新质生产力发展水平的总体差异在缓慢扩大,区域间差异是造成总体差异的主要来源。(3)从时空演进来看,我国新质生产力的多极化现象正在逐渐减少,空间分布呈现出总体向外扩张的特征,空间集聚态势有所减弱,重心迁移上呈现出向东南方向移动的趋势。(4)从驱动因素识别来看,政府支持力度、地区经济发展水平、居民数字化服务消费水平的影响力度强于其他驱动因子,且地区经济发展水平和政府支持力度、其他因子的交互影响是推动我国新质生产力发展的关键。 展开更多
关键词 新质生产力 时空演进 标准差椭圆 驱动因素 地理探测器
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人甲胎蛋白第2代国际标准品协作标定研究
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作者 于婷 曲守方 《标记免疫分析与临床》 2025年第3期599-603,共5页
目的对人甲胎蛋白(alpha-fetoprotein,AFP)第2代国际标准品(编号:22/216)的含量进行协作标定研究。方法中国食品药品检定研究院代表中国实验室,组织了13家AFP试剂生产企业,采用13种化学发光免疫分析法、2种时间分辨荧光免疫法和1种酶联... 目的对人甲胎蛋白(alpha-fetoprotein,AFP)第2代国际标准品(编号:22/216)的含量进行协作标定研究。方法中国食品药品检定研究院代表中国实验室,组织了13家AFP试剂生产企业,采用13种化学发光免疫分析法、2种时间分辨荧光免疫法和1种酶联免疫法的试剂盒,按照英国药品和健康产品管理局提供的统一研究方案,以AFP第1代国际标准品为标准标定22/216。结果中国实验室提交的22/216含量的几何平均值(geometric mean,GM)为7828.78IU/安瓿(95%CI:7462.49~8213.05IU/安瓿),其几何变异系数(geometric coefficient of variation,GCV)为9.77%(n=16)。全球4个国家9个实验室参加了本研究,全部数据得到的22/216含量GM为7756.21IU/安瓿(95%CI:7486.81~8035.30IU/安瓿),GCV为8.73%(n=24)。结论经世界卫生组织生物标准专家委员会审核,确定22/216可作为AFP第2代国际标准品使用,效价为7800IU/安瓿。 展开更多
关键词 甲胎蛋白 国际标准品 协作标定 化学发光免疫分析法 时间分辨荧光免疫法 酶联免疫法
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